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The map label for this gene is glgX [H]

Identifier: 83593909

GI number: 83593909

Start: 2997333

End: 2999354

Strand: Direct

Name: glgX [H]

Synonym: Rru_A2577

Alternate gene names: 83593909

Gene position: 2997333-2999354 (Clockwise)

Preceding gene: 83593908

Following gene: 83593924

Centisome position: 68.86

GC content: 68.1

Gene sequence:

>2022_bases
ATGAGTATCCGCGCCCGGCTGGACGCCGGCGAGCCATGGCCCCTGGGCGCCATGGCTCGCGAGGGCGGCGTCAATATCGC
CGTCTTTTCCGAGGCGGCGACGCAAATCGATCTTTGCCTGTTCGACGGCGACCAGGAAACCCGCCTGCCTTTGCCCGCGC
GCACCGGCGACATCTGGCATGGCTTCCTGCCCGGTGGCGGGCCGGGCTTGCGCTATGGCCTGCGCGCCCACGGCCCCTAT
GATCCGGGCCGGGGTCAGCGCTTCAATCCCCACAAGCTGCTGATCGATCCCGCCGCCCGCCGCCTGGACGGACCGGTGGT
CTGGGACAAGGCGCTTTTTGGCTTCGATCCCGACCATCCGGAACGCGATCTGTCGTTTTCCATCCGCGACAGCGCGGCCT
TCGTGCCCAAGGCGGTGGTCGAGGCCGACCCCGCCACGCGCGCCTTGCGGTCCCCCCCCGATCCCGGCCGCCTGCTGATC
TATGAGGCCCATGTCAAAGGCCTGACCCGCACCCATCCCCAGGTCGCCCCGGAGGCGCGCGGCACCTTTGATGGCGTCGC
CGGATCGGCGGTGATCGCCCATCTCAAGCACATGGGCGTCACCGCCCTGGAACTGCTGCCGGTCTGGGCCTTCGCCGACG
AACCCCACCTGCCGCCGCTCGGGCTGACCAACTATTGGGGCTATAACCCGGTCTGCCTGACCGCCCCCCACGCCGCCTAT
GGCCCGCCCGACTCCTTCCGGCGCATGATCGATTGCCTCCATGGCGCCGATATCGCCGTGATCCTCGATGTCGTTCTCAA
CCATTCGGGCGAGGGCGACGAATACGGACCGACGCTCAGCTTCCGTGGTCTCGACAACGCCGCCTATTACCGCCTCGATC
CCACCGATCCCCGGCGCTACCTCAACGACAGCGCCTGCGGCAACACCCTGCGCATGGAGCATCCCCAGGTCTTGCGGCTG
GCGATGGACGCCTTGCGCTATTGGGTGGGGGTGATGGGGGTGGATGGCTTCCGCTTCGATCTCGCCGTCACTCCGGCCCG
CCACAAGGGGGCTTTCGATCCCAGCGGTCCCTTCCTCAGCGCCATCGCCCAGGATCCGCTGCTCTCGCGCGCCCTGGTGA
TCGCCGAGCCCTGGGATGTCGGCCCCGGTGGCTATCAGGCCGGCCGTTTCCCCAAGTCCTGGATGGAATGGAACGATCTG
GCCCGCGACGGCTTGCGCCGCTTCTGGCTGGCCAATGGCCGATCGGGGGATCTGGCCGCGGTGCTGGCCGGCTGCGCCGC
GCGCTATGCCCCCTCGGGTCGCGGTCCAAGGGCCGGGGTTTCCTATATCAGCGCCCACGACGGCTTCACCCTGGCCGATA
TGGTCAGCTATGGCCATCGCCACAACAACGCCAATGGCGAGAGCAACCGCGACGGATCGGCGATCGAGCTTTCGGCCAAT
CACGGGGTCGAAGGCCCGACCAACGATTTGGCCATCCGCTCGTTACGCGATCGCCAACGGCGCAATCTGCTGGCCTGCCT
GCTGTTGTCGCTGGGCGTGCCGATGATCCGCGGCGGCGATGAACTGGGCCAAACCCAGCAGGGCAACAACAACGTCTATT
GTCAGGATAACCCGCTCAATTGGCTTGATTGGGGCGGCGCCGATGCGAATTTCGCCGCCTTCGCCGCCCGCGCCGGCGCC
CTGCGGGCAAGACTGCCCCAATTGGGCCGGGCGAGTTTTCTCAACGGCTTCCCCGACGCCAAGGGGAAACGCGACGTGGT
CTGGCTGCGCCCCGATGGCGGCGAAATGAGCCCCGCCGATTGGGACGCCCTGCCCCGCTTGCTGGCCATGCGGCTGACCG
GCGCCGAGAAGGCTTCCGACCTGCTGGTGCTGATCAATGGCCAGCCCCACGAGCAATCCTTCCGCCTGCCCCCCGGTCCG
GCCTGGACGATCGCCCTGCGCAGCGCCGGCGACGACGCGCCCGCCGCCGCCGGGTCCAGTCTGGTGGTGCCGGAAATGTC
GCTGGTCGCCCTCACCAGTTAA

Upstream 100 bases:

>100_bases
AGCCACGGTCACGCCCAGTCGGTGGTGCTAACCCTGCCGCCGCTGGCGACCATCGTGCTTCGCCCCGATGGTCCCATCAC
CCGCATCGCCTGACCCGACG

Downstream 100 bases:

>100_bases
AAAGGCTCGCCGATCAGCCCATGGCCCTGGAGCAGCAAGCGCAGGGTTTCCAGGCGGGTCGCCAGTTCCTTGATGTTGCG
GTCATGGGCGGCCAGGGCGG

Product: glycogen debranching protein GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MSIRARLDAGEPWPLGAMAREGGVNIAVFSEAATQIDLCLFDGDQETRLPLPARTGDIWHGFLPGGGPGLRYGLRAHGPY
DPGRGQRFNPHKLLIDPAARRLDGPVVWDKALFGFDPDHPERDLSFSIRDSAAFVPKAVVEADPATRALRSPPDPGRLLI
YEAHVKGLTRTHPQVAPEARGTFDGVAGSAVIAHLKHMGVTALELLPVWAFADEPHLPPLGLTNYWGYNPVCLTAPHAAY
GPPDSFRRMIDCLHGADIAVILDVVLNHSGEGDEYGPTLSFRGLDNAAYYRLDPTDPRRYLNDSACGNTLRMEHPQVLRL
AMDALRYWVGVMGVDGFRFDLAVTPARHKGAFDPSGPFLSAIAQDPLLSRALVIAEPWDVGPGGYQAGRFPKSWMEWNDL
ARDGLRRFWLANGRSGDLAAVLAGCAARYAPSGRGPRAGVSYISAHDGFTLADMVSYGHRHNNANGESNRDGSAIELSAN
HGVEGPTNDLAIRSLRDRQRRNLLACLLLSLGVPMIRGGDELGQTQQGNNNVYCQDNPLNWLDWGGADANFAAFAARAGA
LRARLPQLGRASFLNGFPDAKGKRDVVWLRPDGGEMSPADWDALPRLLAMRLTGAEKASDLLVLINGQPHEQSFRLPPGP
AWTIALRSAGDDAPAAAGSSLVVPEMSLVALTS

Sequences:

>Translated_673_residues
MSIRARLDAGEPWPLGAMAREGGVNIAVFSEAATQIDLCLFDGDQETRLPLPARTGDIWHGFLPGGGPGLRYGLRAHGPY
DPGRGQRFNPHKLLIDPAARRLDGPVVWDKALFGFDPDHPERDLSFSIRDSAAFVPKAVVEADPATRALRSPPDPGRLLI
YEAHVKGLTRTHPQVAPEARGTFDGVAGSAVIAHLKHMGVTALELLPVWAFADEPHLPPLGLTNYWGYNPVCLTAPHAAY
GPPDSFRRMIDCLHGADIAVILDVVLNHSGEGDEYGPTLSFRGLDNAAYYRLDPTDPRRYLNDSACGNTLRMEHPQVLRL
AMDALRYWVGVMGVDGFRFDLAVTPARHKGAFDPSGPFLSAIAQDPLLSRALVIAEPWDVGPGGYQAGRFPKSWMEWNDL
ARDGLRRFWLANGRSGDLAAVLAGCAARYAPSGRGPRAGVSYISAHDGFTLADMVSYGHRHNNANGESNRDGSAIELSAN
HGVEGPTNDLAIRSLRDRQRRNLLACLLLSLGVPMIRGGDELGQTQQGNNNVYCQDNPLNWLDWGGADANFAAFAARAGA
LRARLPQLGRASFLNGFPDAKGKRDVVWLRPDGGEMSPADWDALPRLLAMRLTGAEKASDLLVLINGQPHEQSFRLPPGP
AWTIALRSAGDDAPAAAGSSLVVPEMSLVALTS
>Mature_672_residues
SIRARLDAGEPWPLGAMAREGGVNIAVFSEAATQIDLCLFDGDQETRLPLPARTGDIWHGFLPGGGPGLRYGLRAHGPYD
PGRGQRFNPHKLLIDPAARRLDGPVVWDKALFGFDPDHPERDLSFSIRDSAAFVPKAVVEADPATRALRSPPDPGRLLIY
EAHVKGLTRTHPQVAPEARGTFDGVAGSAVIAHLKHMGVTALELLPVWAFADEPHLPPLGLTNYWGYNPVCLTAPHAAYG
PPDSFRRMIDCLHGADIAVILDVVLNHSGEGDEYGPTLSFRGLDNAAYYRLDPTDPRRYLNDSACGNTLRMEHPQVLRLA
MDALRYWVGVMGVDGFRFDLAVTPARHKGAFDPSGPFLSAIAQDPLLSRALVIAEPWDVGPGGYQAGRFPKSWMEWNDLA
RDGLRRFWLANGRSGDLAAVLAGCAARYAPSGRGPRAGVSYISAHDGFTLADMVSYGHRHNNANGESNRDGSAIELSANH
GVEGPTNDLAIRSLRDRQRRNLLACLLLSLGVPMIRGGDELGQTQQGNNNVYCQDNPLNWLDWGGADANFAAFAARAGAL
RARLPQLGRASFLNGFPDAKGKRDVVWLRPDGGEMSPADWDALPRLLAMRLTGAEKASDLLVLINGQPHEQSFRLPPGPA
WTIALRSAGDDAPAAAGSSLVVPEMSLVALTS

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=660, Percent_Identity=44.8484848484849, Blast_Score=513, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6320826, Length=183, Percent_Identity=28.4153005464481, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28573410, Length=197, Percent_Identity=28.4263959390863, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 72651; Mature: 72520

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRARLDAGEPWPLGAMAREGGVNIAVFSEAATQIDLCLFDGDQETRLPLPARTGDIWH
CCEEEECCCCCCCCCCHHHHCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCEE
GFLPGGGPGLRYGLRAHGPYDPGRGQRFNPHKLLIDPAARRLDGPVVWDKALFGFDPDHP
CCCCCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECHHHHHCCCCEEEHHHHCCCCCCCC
ERDLSFSIRDSAAFVPKAVVEADPATRALRSPPDPGRLLIYEAHVKGLTRTHPQVAPEAR
CCCCEEEECCCCHHCCHHHHCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
GTFDGVAGSAVIAHLKHMGVTALELLPVWAFADEPHLPPLGLTNYWGYNPVCLTAPHAAY
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEECCCCCC
GPPDSFRRMIDCLHGADIAVILDVVLNHSGEGDEYGPTLSFRGLDNAAYYRLDPTDPRRY
CCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEECCCCHHHH
LNDSACGNTLRMEHPQVLRLAMDALRYWVGVMGVDGFRFDLAVTPARHKGAFDPSGPFLS
CCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHCCCCCCCCHHHH
AIAQDPLLSRALVIAEPWDVGPGGYQAGRFPKSWMEWNDLARDGLRRFWLANGRSGDLAA
HHHCCHHHHCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
VLAGCAARYAPSGRGPRAGVSYISAHDGFTLADMVSYGHRHNNANGESNRDGSAIELSAN
HHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECC
HGVEGPTNDLAIRSLRDRQRRNLLACLLLSLGVPMIRGGDELGQTQQGNNNVYCQDNPLN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCEEEECCCCCC
WLDWGGADANFAAFAARAGALRARLPQLGRASFLNGFPDAKGKRDVVWLRPDGGEMSPAD
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCEEEECCCCCCCCCCC
WDALPRLLAMRLTGAEKASDLLVLINGQPHEQSFRLPPGPAWTIALRSAGDDAPAAAGSS
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCE
LVVPEMSLVALTS
EECCCCEEEEECC
>Mature Secondary Structure 
SIRARLDAGEPWPLGAMAREGGVNIAVFSEAATQIDLCLFDGDQETRLPLPARTGDIWH
CEEEECCCCCCCCCCHHHHCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCEE
GFLPGGGPGLRYGLRAHGPYDPGRGQRFNPHKLLIDPAARRLDGPVVWDKALFGFDPDHP
CCCCCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECHHHHHCCCCEEEHHHHCCCCCCCC
ERDLSFSIRDSAAFVPKAVVEADPATRALRSPPDPGRLLIYEAHVKGLTRTHPQVAPEAR
CCCCEEEECCCCHHCCHHHHCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
GTFDGVAGSAVIAHLKHMGVTALELLPVWAFADEPHLPPLGLTNYWGYNPVCLTAPHAAY
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEECCCCCC
GPPDSFRRMIDCLHGADIAVILDVVLNHSGEGDEYGPTLSFRGLDNAAYYRLDPTDPRRY
CCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEECCCCHHHH
LNDSACGNTLRMEHPQVLRLAMDALRYWVGVMGVDGFRFDLAVTPARHKGAFDPSGPFLS
CCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHCCCCCCCCHHHH
AIAQDPLLSRALVIAEPWDVGPGGYQAGRFPKSWMEWNDLARDGLRRFWLANGRSGDLAA
HHHCCHHHHCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
VLAGCAARYAPSGRGPRAGVSYISAHDGFTLADMVSYGHRHNNANGESNRDGSAIELSAN
HHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECC
HGVEGPTNDLAIRSLRDRQRRNLLACLLLSLGVPMIRGGDELGQTQQGNNNVYCQDNPLN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCEEEECCCCCC
WLDWGGADANFAAFAARAGALRARLPQLGRASFLNGFPDAKGKRDVVWLRPDGGEMSPAD
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCEEEECCCCCCCCCCC
WDALPRLLAMRLTGAEKASDLLVLINGQPHEQSFRLPPGPAWTIALRSAGDDAPAAAGSS
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCE
LVVPEMSLVALTS
EECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]