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The map label for this gene is glgB

Identifier: 83593908

GI number: 83593908

Start: 2995103

End: 2997325

Strand: Direct

Name: glgB

Synonym: Rru_A2576

Alternate gene names: 83593908

Gene position: 2995103-2997325 (Clockwise)

Preceding gene: 83593907

Following gene: 83593909

Centisome position: 68.81

GC content: 63.47

Gene sequence:

>2223_bases
ATGGACCCGAAAGCTTATTCCGAAGCCGTGGCCGCCCTTGTCGGGGCCCGCCACAGCAATCCCTTCGCCTTCCTCGGCCC
CCATGAGGGCCGGGCCGAAGGGGGACGCGGCGGCTTCTGCATCCGCTGTTTCCGCCCCAAGGCGACGGCGGTCTCGTTGA
TCTCGGCCGCTGATGACACGGTTCTTGGACGGATGAAGCGCCTTCATCCCGACGGACTGTTCGGCATCGACCTGCCGGAG
GCTCCCGGAGCTCTGGCCTATCGTCTGCGCGTCAGCGAAGGCGACGAGGATCGCGATATCGAAGATCCCTATCGCTTCGG
ACCGGTTCTTGGCGAACTCGACCGCCATCTTCTGGGCGAGGGCACCCACCTCGACATCTATCGCAAGATGGGCGCCCATC
CGATGACCCGCGACGGCGTTCGCGGCACCGGCTTCGCGCTCTGGGCGCCCAATGCCACCCGGGTCAGCGTCATCGGCGAT
TTCAACGGCTGGGATGGCCGGCTTCACCCGATGCGCGCCCATCCGGGCAGCGGGGTGTGGGATATCTTTCTGCCCGGCGT
GGTCGAAGGCCATCTCTATAAGTACGAATTGCTGGGCCCCGACGGCTCCTTGCTGCCGGCCAAGGCCGATCCTTACGCCT
TCCAGGCGGAAAAGCCGCCCCATACCGCTTCGGTGGTGCGCGGCCTGGGAACCTATGCGTGGAACGACGGTCGCTGGATG
AGCGAGCGGGCCGACCGCGTCGCCACGAGCGCGCCGGTGTCGATCTACGAAGTTCACCTCGGCTCGTGGCGCCATGGCGG
CGGCGACCGGTCGCTGAGCTACCGGGAAATGGCCGAGCAGCTGATCCCCTATGTCAAGGAGATGGGGTTCACCCATATCG
AACTGCTGCCGGTCAGCGAGTTCCCCTTCGATGGCTCCTGGGGCTATCAGCCGATCGGCCTGTTCGCGCCGACCAGCCGC
TTTGGCGAACCCGATGATTTCCGCCATTTCGTCGACCGCTGCCATCAGGAAGGCGTCGGCGTCATCCTGGATTGGGTGGC
CGGCCACTTCCCCGAAGACGCCCACGGCCTGTCGTGGTTCGATGGCACCCATCTTTATGAGCATTCCGACCCGCGCCAGG
GCCGGCATATGGATTGGGGCACCTATATCTTCAATTACGGCCGCAACGAGGTTCGCAACTTCCTGCTGGCCAACGCCCTG
TTCTGGCTTGAGCAATTCCACATCGACGGCCTGCGCGTCGATGCCGTCGCCTCGATGCTCTATCTTGATTATTCGCGCAA
GGCCGGCGAATGGGTCCCCAATAAGTTCGGCGGCCGCGAAAACCTGGAAGCCATCGATTTCCTGCGCCGAATGAACGAGC
TGGTCTATGGCCGCTTCCCCGGCGCCGTCACCATCGCCGAGGAAAGCACCGCTTGGCCGATGGTCAGCCGTCCCGTTCAT
CTGGGCGGTCTGGGCTTTGGCTATAAGTGGAACATGGGCTGGATGAACGACACGCTCAGCTACATGTCGCAAGATCCGAT
CTATCGCCGTTTCCATCAGCATGATCTGAGCTTCGGCCTGCTCTACGCCTTCACCGAGAACTTCGTTCTGCCGCTTTCCC
ACGACGAGGTCGTCCACGGCAAGCGCTCGATCCTCGGACGGATGCCCGGCGACGCGTGGCAACAGTTCGCCAATCTGCGG
GCCTATTACGGCTTCATGTGGACCCATCCGGGCAAGAAACTGCTGTTCATGGGCTGCGAATTCGCCCAGGGGCGCGAGTG
GAACCACAACGCCAGTCTCGATTGGCATCTGCTCGATATCGACTGGCATAAGGGCGTTCAGGCGCTGGTGCGCGATCTCA
ACGGGCTGTATGCCGGGGTGCCCGCCCTCCACGACCGCGATACCGAAGGCTACGGGTTCTCCTGGATCGACTGCACCGAT
GCCGATCAATCGGTTCTGGCCTTCCTGCGCTTTGGCGAAACGGCCGAGGACGTGGTGATGGTGGTTTGCAATTTCACCCC
CAACCCGCGCCATGGCTATCGCCTGGGCGCGCCGATCGCCGGCCGCTGGCGCGAGATCTTCAATACCGACAGCGCCCATT
ACGGCGGCTCCAATATGGGAAATTCGGTGGTGGAGACCGAGGAGACCAAAAGCCACGGTCACGCCCAGTCGGTGGTGCTA
ACCCTGCCGCCGCTGGCGACCATCGTGCTTCGCCCCGATGGTCCCATCACCCGCATCGCCTGA

Upstream 100 bases:

>100_bases
TGGCGGCGCGGGTTCGGGAACGATGGGGAACCTGTCCCCCCTTCCGCGCGTTGGGTCGATACACCGTGGACACATCCTGA
TACCCCAAGCGGGAGTCCCA

Downstream 100 bases:

>100_bases
CCCGACGATGAGTATCCGCGCCCGGCTGGACGCCGGCGAGCCATGGCCCCTGGGCGCCATGGCTCGCGAGGGCGGCGTCA
ATATCGCCGTCTTTTCCGAG

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 740; Mature: 740

Protein sequence:

>740_residues
MDPKAYSEAVAALVGARHSNPFAFLGPHEGRAEGGRGGFCIRCFRPKATAVSLISAADDTVLGRMKRLHPDGLFGIDLPE
APGALAYRLRVSEGDEDRDIEDPYRFGPVLGELDRHLLGEGTHLDIYRKMGAHPMTRDGVRGTGFALWAPNATRVSVIGD
FNGWDGRLHPMRAHPGSGVWDIFLPGVVEGHLYKYELLGPDGSLLPAKADPYAFQAEKPPHTASVVRGLGTYAWNDGRWM
SERADRVATSAPVSIYEVHLGSWRHGGGDRSLSYREMAEQLIPYVKEMGFTHIELLPVSEFPFDGSWGYQPIGLFAPTSR
FGEPDDFRHFVDRCHQEGVGVILDWVAGHFPEDAHGLSWFDGTHLYEHSDPRQGRHMDWGTYIFNYGRNEVRNFLLANAL
FWLEQFHIDGLRVDAVASMLYLDYSRKAGEWVPNKFGGRENLEAIDFLRRMNELVYGRFPGAVTIAEESTAWPMVSRPVH
LGGLGFGYKWNMGWMNDTLSYMSQDPIYRRFHQHDLSFGLLYAFTENFVLPLSHDEVVHGKRSILGRMPGDAWQQFANLR
AYYGFMWTHPGKKLLFMGCEFAQGREWNHNASLDWHLLDIDWHKGVQALVRDLNGLYAGVPALHDRDTEGYGFSWIDCTD
ADQSVLAFLRFGETAEDVVMVVCNFTPNPRHGYRLGAPIAGRWREIFNTDSAHYGGSNMGNSVVETEETKSHGHAQSVVL
TLPPLATIVLRPDGPITRIA

Sequences:

>Translated_740_residues
MDPKAYSEAVAALVGARHSNPFAFLGPHEGRAEGGRGGFCIRCFRPKATAVSLISAADDTVLGRMKRLHPDGLFGIDLPE
APGALAYRLRVSEGDEDRDIEDPYRFGPVLGELDRHLLGEGTHLDIYRKMGAHPMTRDGVRGTGFALWAPNATRVSVIGD
FNGWDGRLHPMRAHPGSGVWDIFLPGVVEGHLYKYELLGPDGSLLPAKADPYAFQAEKPPHTASVVRGLGTYAWNDGRWM
SERADRVATSAPVSIYEVHLGSWRHGGGDRSLSYREMAEQLIPYVKEMGFTHIELLPVSEFPFDGSWGYQPIGLFAPTSR
FGEPDDFRHFVDRCHQEGVGVILDWVAGHFPEDAHGLSWFDGTHLYEHSDPRQGRHMDWGTYIFNYGRNEVRNFLLANAL
FWLEQFHIDGLRVDAVASMLYLDYSRKAGEWVPNKFGGRENLEAIDFLRRMNELVYGRFPGAVTIAEESTAWPMVSRPVH
LGGLGFGYKWNMGWMNDTLSYMSQDPIYRRFHQHDLSFGLLYAFTENFVLPLSHDEVVHGKRSILGRMPGDAWQQFANLR
AYYGFMWTHPGKKLLFMGCEFAQGREWNHNASLDWHLLDIDWHKGVQALVRDLNGLYAGVPALHDRDTEGYGFSWIDCTD
ADQSVLAFLRFGETAEDVVMVVCNFTPNPRHGYRLGAPIAGRWREIFNTDSAHYGGSNMGNSVVETEETKSHGHAQSVVL
TLPPLATIVLRPDGPITRIA
>Mature_740_residues
MDPKAYSEAVAALVGARHSNPFAFLGPHEGRAEGGRGGFCIRCFRPKATAVSLISAADDTVLGRMKRLHPDGLFGIDLPE
APGALAYRLRVSEGDEDRDIEDPYRFGPVLGELDRHLLGEGTHLDIYRKMGAHPMTRDGVRGTGFALWAPNATRVSVIGD
FNGWDGRLHPMRAHPGSGVWDIFLPGVVEGHLYKYELLGPDGSLLPAKADPYAFQAEKPPHTASVVRGLGTYAWNDGRWM
SERADRVATSAPVSIYEVHLGSWRHGGGDRSLSYREMAEQLIPYVKEMGFTHIELLPVSEFPFDGSWGYQPIGLFAPTSR
FGEPDDFRHFVDRCHQEGVGVILDWVAGHFPEDAHGLSWFDGTHLYEHSDPRQGRHMDWGTYIFNYGRNEVRNFLLANAL
FWLEQFHIDGLRVDAVASMLYLDYSRKAGEWVPNKFGGRENLEAIDFLRRMNELVYGRFPGAVTIAEESTAWPMVSRPVH
LGGLGFGYKWNMGWMNDTLSYMSQDPIYRRFHQHDLSFGLLYAFTENFVLPLSHDEVVHGKRSILGRMPGDAWQQFANLR
AYYGFMWTHPGKKLLFMGCEFAQGREWNHNASLDWHLLDIDWHKGVQALVRDLNGLYAGVPALHDRDTEGYGFSWIDCTD
ADQSVLAFLRFGETAEDVVMVVCNFTPNPRHGYRLGAPIAGRWREIFNTDSAHYGGSNMGNSVVETEETKSHGHAQSVVL
TLPPLATIVLRPDGPITRIA

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=661, Percent_Identity=26.928895612708, Blast_Score=197, Evalue=3e-50,
Organism=Escherichia coli, GI1789839, Length=734, Percent_Identity=56.9482288828338, Blast_Score=826, Evalue=0.0,
Organism=Escherichia coli, GI2367229, Length=344, Percent_Identity=27.3255813953488, Blast_Score=69, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17554896, Length=664, Percent_Identity=26.355421686747, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI32564391, Length=548, Percent_Identity=26.8248175182482, Blast_Score=158, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6320826, Length=397, Percent_Identity=32.2418136020151, Blast_Score=180, Evalue=7e-46,
Organism=Drosophila melanogaster, GI28573410, Length=607, Percent_Identity=28.995057660626, Blast_Score=190, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_RHORT (Q2RR72)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_427660.1
- ProteinModelPortal:   Q2RR72
- SMR:   Q2RR72
- STRING:   Q2RR72
- GeneID:   3836011
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A2576
- NMPDR:   fig|1085.1.peg.227
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   DGTCLYE
- PhylomeDB:   Q2RR72
- ProtClustDB:   PRK05402
- BioCyc:   RRUB269796:RRU_A2576-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 83076; Mature: 83076

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: ACT_SITE 309-309 ACT_SITE 344-344 ACT_SITE 349-349 ACT_SITE 412-412 ACT_SITE 414-414 ACT_SITE 467-467 ACT_SITE 534-534 ACT_SITE 535-535

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPKAYSEAVAALVGARHSNPFAFLGPHEGRAEGGRGGFCIRCFRPKATAVSLISAADDT
CCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCHH
VLGRMKRLHPDGLFGIDLPEAPGALAYRLRVSEGDEDRDIEDPYRFGPVLGELDRHLLGE
HHHHHHHCCCCCEEECCCCCCCCCEEEEEEECCCCCCCCCCCCHHHCHHHHHHHHHHHCC
GTHLDIYRKMGAHPMTRDGVRGTGFALWAPNATRVSVIGDFNGWDGRLHPMRAHPGSGVW
CCEEHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCEEEEEECCCCCCEE
DIFLPGVVEGHLYKYELLGPDGSLLPAKADPYAFQAEKPPHTASVVRGLGTYAWNDGRWM
EEECCCEECCCEEEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCEEECCCCCH
SERADRVATSAPVSIYEVHLGSWRHGGGDRSLSYREMAEQLIPYVKEMGFTHIELLPVSE
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
FPFDGSWGYQPIGLFAPTSRFGEPDDFRHFVDRCHQEGVGVILDWVAGHFPEDAHGLSWF
CCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCCC
DGTHLYEHSDPRQGRHMDWGTYIFNYGRNEVRNFLLANALFWLEQFHIDGLRVDAVASML
CCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHH
YLDYSRKAGEWVPNKFGGRENLEAIDFLRRMNELVYGRFPGAVTIAEESTAWPMVSRPVH
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCEEEEECCCCCCCCCCCCEE
LGGLGFGYKWNMGWMNDTLSYMSQDPIYRRFHQHDLSFGLLYAFTENFVLPLSHDEVVHG
ECCCCCCEEECCCCHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCEEEECCCCHHHHC
KRSILGRMPGDAWQQFANLRAYYGFMWTHPGKKLLFMGCEFAQGREWNHNASLDWHLLDI
HHHHHCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEEEE
DWHKGVQALVRDLNGLYAGVPALHDRDTEGYGFSWIDCTDADQSVLAFLRFGETAEDVVM
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEE
VVCNFTPNPRHGYRLGAPIAGRWREIFNTDSAHYGGSNMGNSVVETEETKSHGHAQSVVL
EEEECCCCCCCCEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCEECCHHHCCCCCEEEEE
TLPPLATIVLRPDGPITRIA
EECCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MDPKAYSEAVAALVGARHSNPFAFLGPHEGRAEGGRGGFCIRCFRPKATAVSLISAADDT
CCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCHH
VLGRMKRLHPDGLFGIDLPEAPGALAYRLRVSEGDEDRDIEDPYRFGPVLGELDRHLLGE
HHHHHHHCCCCCEEECCCCCCCCCEEEEEEECCCCCCCCCCCCHHHCHHHHHHHHHHHCC
GTHLDIYRKMGAHPMTRDGVRGTGFALWAPNATRVSVIGDFNGWDGRLHPMRAHPGSGVW
CCEEHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCEEEEEECCCCCCEE
DIFLPGVVEGHLYKYELLGPDGSLLPAKADPYAFQAEKPPHTASVVRGLGTYAWNDGRWM
EEECCCEECCCEEEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCEEECCCCCH
SERADRVATSAPVSIYEVHLGSWRHGGGDRSLSYREMAEQLIPYVKEMGFTHIELLPVSE
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
FPFDGSWGYQPIGLFAPTSRFGEPDDFRHFVDRCHQEGVGVILDWVAGHFPEDAHGLSWF
CCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCCC
DGTHLYEHSDPRQGRHMDWGTYIFNYGRNEVRNFLLANALFWLEQFHIDGLRVDAVASML
CCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHH
YLDYSRKAGEWVPNKFGGRENLEAIDFLRRMNELVYGRFPGAVTIAEESTAWPMVSRPVH
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCEEEEECCCCCCCCCCCCEE
LGGLGFGYKWNMGWMNDTLSYMSQDPIYRRFHQHDLSFGLLYAFTENFVLPLSHDEVVHG
ECCCCCCEEECCCCHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCEEEECCCCHHHHC
KRSILGRMPGDAWQQFANLRAYYGFMWTHPGKKLLFMGCEFAQGREWNHNASLDWHLLDI
HHHHHCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEEEE
DWHKGVQALVRDLNGLYAGVPALHDRDTEGYGFSWIDCTDADQSVLAFLRFGETAEDVVM
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEE
VVCNFTPNPRHGYRLGAPIAGRWREIFNTDSAHYGGSNMGNSVVETEETKSHGHAQSVVL
EEEECCCCCCCCEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCEECCHHHCCCCCEEEEE
TLPPLATIVLRPDGPITRIA
EECCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA