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The map label for this gene is pyk [H]

Identifier: 83593800

GI number: 83593800

Start: 2864811

End: 2866247

Strand: Direct

Name: pyk [H]

Synonym: Rru_A2465

Alternate gene names: 83593800

Gene position: 2864811-2866247 (Clockwise)

Preceding gene: 83593799

Following gene: 83593801

Centisome position: 65.81

GC content: 65.55

Gene sequence:

>1437_bases
ATGAAACGAAACCGTTGCGCCAAGATTCTGGCCACCCTCGGCCCGGCCACGTCCTCGGAAAGCACCCTCGAGACCCTGGT
TCGGGCCGGTGCGGACATGGTTCGCCTGAATTTCAGCCACGGCGCCCACGAAGAACACCGGCGCCGCTACGACGTGGTCC
GCGCCGTTGAAAACAAGCTCGGCCGACCGATCGGCATCCTGATGGACCTCCAGGGTCCGAAGCTGCGGGTCGGTGATTTC
GTCGGCGGCAAGGTCAAGCTGGAAACCGGCGCCACCTTCCGTCTCGACATGGAAAAGGACCTGGGCACCATCCAGCGTGT
CCGCCTGCCCCACCCCGAAATCTTCGCCGCCATCGGCGAAGGCACCGATCTGTTGCTTGACGACGGCAAACTGCGGCTGC
GGGTGGAAACCTTCGGCGCCGATTACGCCATTACCCGGGTGATCACCGGCGGCGTGCTGTCGGACCACAAGGGCCTCAAT
GTGCCCAATGCCGTTCTGCCGATCTCGGCGCTGACCGAAAAGGACCGCCGCGACCTGCAGTTCGGCCTCGACCTCGGGGT
CGATTGGGTTGGCCTCAGCTTCGTGCAGCGCCCCGAGGACGTGGCCGAGGCGCGCAAGCTGATCGGCGGTCGCGCCGCCA
TCATCAGCAAGATCGAAAAGCCCGCCGCCCTCGACCATCTCACCCATATCATCGAATTGTCCGACGCCCTGATGGTCGCC
CGCGGCGATCTGGGCGTGGAAGTGCCGCCCGAGCGCGTGCCGATCTTGCAAAAGCGCATCCTGGCCGCCTGCCACCTCGC
CGGCAAGCCGGTGGTGGTCGCCACCCAGATGCTTGAAAGCATGATCAACGCCCCGACGCCGACCCGCGCCGAGGCCTCGG
ACGTGGCGACGGCCATCTATGATGGCGCCGATGCGGTGATGCTGTCGGCGGAAACCGCCGTCGGCCAGTATCCGGTCGAA
GCCGTGGCGATGATGGACAAGATCATCCAGCAGGTGGAAACCGACGAGCATTATCAGTATCTGCGCAATGCCCGCCGCGA
AAGCCCCGAGGGCACGGCCGCCGACGCCATCAGCGCCGCCGCCAGTCAGGTGGCCGAGACCATCAAGGCCGCCGCCATCG
TCACCTATACCTCCTCGGGATCGACGACGCTGCGCGCCGCCCGCCAGCGCCCCGGCGTGCCGATCCTTTGCCTGACCACC
AACCCCCGCATCGCCCGACGGATGGCGATGGTCTGGGGCGTCCATTCGGTGCTCGACAAGGACATCTCCAACTTCCAGGA
AATGGTCGAACTGGCGGTCAAGGCCGCCTATCGCGAAGGCTTCGCCAATATCGGCGAGCGCATCGTGATGACGGCGGGCG
TGCCCTTTGGCACCCCGGGCACGACCAATGTTCTGCGAATCGCCTGGGTCGAAGGCGATGACAGCTGGGCGGAATAA

Upstream 100 bases:

>100_bases
TGATGTTAAAAAATACAACGGTGGCCCCATGCCGCCACATCCCCCCTCCCTAGCTCGGTGACGCGCCCCGTCACAGGGCC
CAAGCCCGTGTGAACGCTCC

Downstream 100 bases:

>100_bases
GCCCGCCTGTTTACCGACTCACTCCCGGGCGGGCGATGCCCTCGCCCGTCCGGGACCATGACCCGCGCGAGGCCCCCCTC
GCGCCCAGTCCGGGACTTTT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKRNRCAKILATLGPATSSESTLETLVRAGADMVRLNFSHGAHEEHRRRYDVVRAVENKLGRPIGILMDLQGPKLRVGDF
VGGKVKLETGATFRLDMEKDLGTIQRVRLPHPEIFAAIGEGTDLLLDDGKLRLRVETFGADYAITRVITGGVLSDHKGLN
VPNAVLPISALTEKDRRDLQFGLDLGVDWVGLSFVQRPEDVAEARKLIGGRAAIISKIEKPAALDHLTHIIELSDALMVA
RGDLGVEVPPERVPILQKRILAACHLAGKPVVVATQMLESMINAPTPTRAEASDVATAIYDGADAVMLSAETAVGQYPVE
AVAMMDKIIQQVETDEHYQYLRNARRESPEGTAADAISAAASQVAETIKAAAIVTYTSSGSTTLRAARQRPGVPILCLTT
NPRIARRMAMVWGVHSVLDKDISNFQEMVELAVKAAYREGFANIGERIVMTAGVPFGTPGTTNVLRIAWVEGDDSWAE

Sequences:

>Translated_478_residues
MKRNRCAKILATLGPATSSESTLETLVRAGADMVRLNFSHGAHEEHRRRYDVVRAVENKLGRPIGILMDLQGPKLRVGDF
VGGKVKLETGATFRLDMEKDLGTIQRVRLPHPEIFAAIGEGTDLLLDDGKLRLRVETFGADYAITRVITGGVLSDHKGLN
VPNAVLPISALTEKDRRDLQFGLDLGVDWVGLSFVQRPEDVAEARKLIGGRAAIISKIEKPAALDHLTHIIELSDALMVA
RGDLGVEVPPERVPILQKRILAACHLAGKPVVVATQMLESMINAPTPTRAEASDVATAIYDGADAVMLSAETAVGQYPVE
AVAMMDKIIQQVETDEHYQYLRNARRESPEGTAADAISAAASQVAETIKAAAIVTYTSSGSTTLRAARQRPGVPILCLTT
NPRIARRMAMVWGVHSVLDKDISNFQEMVELAVKAAYREGFANIGERIVMTAGVPFGTPGTTNVLRIAWVEGDDSWAE
>Mature_478_residues
MKRNRCAKILATLGPATSSESTLETLVRAGADMVRLNFSHGAHEEHRRRYDVVRAVENKLGRPIGILMDLQGPKLRVGDF
VGGKVKLETGATFRLDMEKDLGTIQRVRLPHPEIFAAIGEGTDLLLDDGKLRLRVETFGADYAITRVITGGVLSDHKGLN
VPNAVLPISALTEKDRRDLQFGLDLGVDWVGLSFVQRPEDVAEARKLIGGRAAIISKIEKPAALDHLTHIIELSDALMVA
RGDLGVEVPPERVPILQKRILAACHLAGKPVVVATQMLESMINAPTPTRAEASDVATAIYDGADAVMLSAETAVGQYPVE
AVAMMDKIIQQVETDEHYQYLRNARRESPEGTAADAISAAASQVAETIKAAAIVTYTSSGSTTLRAARQRPGVPILCLTT
NPRIARRMAMVWGVHSVLDKDISNFQEMVELAVKAAYREGFANIGERIVMTAGVPFGTPGTTNVLRIAWVEGDDSWAE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=491, Percent_Identity=39.3075356415479, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=38.4928716904277, Blast_Score=312, Evalue=5e-85,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=38.4928716904277, Blast_Score=312, Evalue=5e-85,
Organism=Homo sapiens, GI10835121, Length=486, Percent_Identity=38.2716049382716, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI32967597, Length=486, Percent_Identity=38.2716049382716, Blast_Score=309, Evalue=3e-84,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=39.1003460207612, Blast_Score=195, Evalue=8e-50,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=39.1003460207612, Blast_Score=195, Evalue=8e-50,
Organism=Homo sapiens, GI310128736, Length=232, Percent_Identity=37.5, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI310128734, Length=232, Percent_Identity=37.5, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI310128738, Length=211, Percent_Identity=36.9668246445498, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1787965, Length=465, Percent_Identity=38.9247311827957, Blast_Score=307, Evalue=8e-85,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=40.625, Blast_Score=298, Evalue=7e-82,
Organism=Caenorhabditis elegans, GI17544584, Length=484, Percent_Identity=35.9504132231405, Blast_Score=306, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI71984413, Length=489, Percent_Identity=36.8098159509202, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI71984406, Length=492, Percent_Identity=36.7886178861789, Blast_Score=279, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17506829, Length=489, Percent_Identity=36.8098159509202, Blast_Score=279, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17506831, Length=488, Percent_Identity=36.8852459016393, Blast_Score=278, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=35.6701030927835, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6324923, Length=486, Percent_Identity=36.0082304526749, Blast_Score=256, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24648964, Length=490, Percent_Identity=37.9591836734694, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=37.9591836734694, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24648966, Length=430, Percent_Identity=38.1395348837209, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24581235, Length=491, Percent_Identity=32.3828920570265, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24646914, Length=263, Percent_Identity=41.06463878327, Blast_Score=188, Evalue=6e-48,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51760; Mature: 51760

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNRCAKILATLGPATSSESTLETLVRAGADMVRLNFSHGAHEEHRRRYDVVRAVENKL
CCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
GRPIGILMDLQGPKLRVGDFVGGKVKLETGATFRLDMEKDLGTIQRVRLPHPEIFAAIGE
CCCEEEEEECCCCCEEECCCCCCEEEEECCCEEEEECHHCCCHHHHHCCCCHHHHHHHCC
GTDLLLDDGKLRLRVETFGADYAITRVITGGVLSDHKGLNVPNAVLPISALTEKDRRDLQ
CCEEEEECCEEEEEEEECCCCCEEHHHHHCCCCCCCCCCCCCCHHCCHHHHHHCHHHHHH
FGLDLGVDWVGLSFVQRPEDVAEARKLIGGRAAIISKIEKPAALDHLTHIIELSDALMVA
HHHCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEEE
RGDLGVEVPPERVPILQKRILAACHLAGKPVVVATQMLESMINAPTPTRAEASDVATAIY
ECCCCCCCCCHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
DGADAVMLSAETAVGQYPVEAVAMMDKIIQQVETDEHYQYLRNARRESPEGTAADAISAA
CCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHH
ASQVAETIKAAAIVTYTSSGSTTLRAARQRPGVPILCLTTNPRIARRMAMVWGVHSVLDK
HHHHHHHHHHEEEEEEECCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
DISNFQEMVELAVKAAYREGFANIGERIVMTAGVPFGTPGTTNVLRIAWVEGDDSWAE
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MKRNRCAKILATLGPATSSESTLETLVRAGADMVRLNFSHGAHEEHRRRYDVVRAVENKL
CCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
GRPIGILMDLQGPKLRVGDFVGGKVKLETGATFRLDMEKDLGTIQRVRLPHPEIFAAIGE
CCCEEEEEECCCCCEEECCCCCCEEEEECCCEEEEECHHCCCHHHHHCCCCHHHHHHHCC
GTDLLLDDGKLRLRVETFGADYAITRVITGGVLSDHKGLNVPNAVLPISALTEKDRRDLQ
CCEEEEECCEEEEEEEECCCCCEEHHHHHCCCCCCCCCCCCCCHHCCHHHHHHCHHHHHH
FGLDLGVDWVGLSFVQRPEDVAEARKLIGGRAAIISKIEKPAALDHLTHIIELSDALMVA
HHHCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEEE
RGDLGVEVPPERVPILQKRILAACHLAGKPVVVATQMLESMINAPTPTRAEASDVATAIY
ECCCCCCCCCHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
DGADAVMLSAETAVGQYPVEAVAMMDKIIQQVETDEHYQYLRNARRESPEGTAADAISAA
CCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHH
ASQVAETIKAAAIVTYTSSGSTTLRAARQRPGVPILCLTTNPRIARRMAMVWGVHSVLDK
HHHHHHHHHHEEEEEEECCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
DISNFQEMVELAVKAAYREGFANIGERIVMTAGVPFGTPGTTNVLRIAWVEGDDSWAE
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA