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The map label for this gene is aglA [H]

Identifier: 83593629

GI number: 83593629

Start: 2653823

End: 2655451

Strand: Direct

Name: aglA [H]

Synonym: Rru_A2294

Alternate gene names: 83593629

Gene position: 2653823-2655451 (Clockwise)

Preceding gene: 83593626

Following gene: 83593630

Centisome position: 60.97

GC content: 66.48

Gene sequence:

>1629_bases
ATGTCGGGTCAGGAATGGTGGAAACGGGCCGTTATCTATCAAATCTATCCGCGCAGTTTCCAGGATGCCGATGGCGATGG
CATCGGCGATTTGGCCGGCATCGCCCAACGCCTGGATTACCTGTCGTGGCTGGGGGTGGACGCGATCTGGATCTCGCCCT
TCTATACCTCGCCGATGAAGGATTTCGGCTACGACATTCAAGACTACCGCGATGTCGATCCGATCTTCGGCACCCTGGCC
GATTTCGACGCCCTGGCGGCGCGGGCCCGCGCGCTGGGGCTGAAGCTGATCTTGGATTTCGTTCCCAACCACACCTCTGA
TCGGCATCCGTGGTTTCTCGAATCCCGCCAATCGCCCGCCAGCGCCAAGCGCGACTGGTACATCTGGCGCGACCCGGCCG
CCGATGGCGGCCCGCCGACCAACTGGCTGGCCGAATTCGGCGGCAGCGCCTGGGAATACGACGAAGCCAGCGGCCAGTAT
TACTACCACGCCTTCCTCAAGGAGCAGCCCGACCTCAATTGGCGCAATCCAGAAGTCCGCGCGGCGATGTATGACGCCAT
GCGCTTTTGGCTCGAGCGCGGGGTGGATGGCTTCCGCGTCGATGTGATGTGGCATCTGATCAAGGACGACCAGTTCCGCG
ACAATCCGCCCAATCCGGCCCGCCCGCCCAATGCCGGTCCGGCCAATGCCCTGATCCCGCTCTACACCGCCGATCGCCCC
GAAGTGCACGACATCGTGCGCGAGATGCGCGCCGTGGTCGACGCCTATCCCGAACGCGTGCTGATCGGCGAGATCTGGCT
GCCCACCGATCGCCTGGTCACCTATTACGGCCATGATCTGCGCGGTGCCCATCTGCCCTTTAATTTCCAATTGATCGGCG
CCGCCTGGGACGCCGGCGTCATCAAAGAGATCGTCGGCGACTACGAAAAGGCCCTGCCCCGGGGCGGCTGGCCCAATTGG
GTGATGGGCAATCACGACAAGGGCCGCATCGCCAGCCGCCTGGGCCCCCGGCAGGCCCGGCTGGCCATGCTGCTGCTGCT
CACCCTGCGCGGCACCCCCACCCTTTACTATGGCGACGAGATCGCCATGCGCGACGTGCCGATCCCCCCCGACCGGGTCT
GCGATCCTTACGAAAAGAACGTGCCCGGCCTGGGTCTGGGGCGCGACCCCCAGCGCACGCCGATGCAATGGGAGGACGCT
CCCCAGGCCGGCTTCACCCGCGCTTCCCGATCCTGGTTGCCGATCGCCGCCGATGCCTCAACGGTCAATGTCGCCGCCCA
ACGCGCGGCGCCGGCCTCGATGCTGACCCTCGTCCACCGCCTGATCGCCCTGCGCAAAGCCGAAGCGGCCCTAACTATCG
GCGATTATGGGGCGCTCGACGCTCCCGAGCGGGTGGTGATGTTCCTGCGCCGCGTTCCCGGGCGCCGCCTGCTCATCGCC
CTCAATCTGGGGGAAAGCGCCCAAAGCCTGAGCCTGCCCGGCGACAGCCGCCAAGCCACCACCCTGCTGTCCACCGACCC
GGAGCGCCCGCTGGGCGATCTGGCCCCGGCCTTCACCCTTGGCGGGGGCGAGGGCATCGTCGTTTTGCTGGCCGACCGGG
AAACGGCCCCCCCGTCCCCGGCGGCGTAA

Upstream 100 bases:

>100_bases
CGTTGCCTCCCGCACCCGAGTAATTTCCCAAGACAACGGGAAAAATCCACCTCGGCGTTGTTTGGTCACTCAAAGACGGC
GTTGAAGGAGAACCACCCCG

Downstream 100 bases:

>100_bases
TGGGAGGAAAAAACAATGGAGAGCCCCCTGACCCCGCCCGTGGCGCCGGCGCCACGCCCCCCCCTTTCCTCCCTCGTCGC
GGCGCCCCCCGCCGAAGATC

Product: alpha amylase, catalytic region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MSGQEWWKRAVIYQIYPRSFQDADGDGIGDLAGIAQRLDYLSWLGVDAIWISPFYTSPMKDFGYDIQDYRDVDPIFGTLA
DFDALAARARALGLKLILDFVPNHTSDRHPWFLESRQSPASAKRDWYIWRDPAADGGPPTNWLAEFGGSAWEYDEASGQY
YYHAFLKEQPDLNWRNPEVRAAMYDAMRFWLERGVDGFRVDVMWHLIKDDQFRDNPPNPARPPNAGPANALIPLYTADRP
EVHDIVREMRAVVDAYPERVLIGEIWLPTDRLVTYYGHDLRGAHLPFNFQLIGAAWDAGVIKEIVGDYEKALPRGGWPNW
VMGNHDKGRIASRLGPRQARLAMLLLLTLRGTPTLYYGDEIAMRDVPIPPDRVCDPYEKNVPGLGLGRDPQRTPMQWEDA
PQAGFTRASRSWLPIAADASTVNVAAQRAAPASMLTLVHRLIALRKAEAALTIGDYGALDAPERVVMFLRRVPGRRLLIA
LNLGESAQSLSLPGDSRQATTLLSTDPERPLGDLAPAFTLGGGEGIVVLLADRETAPPSPAA

Sequences:

>Translated_542_residues
MSGQEWWKRAVIYQIYPRSFQDADGDGIGDLAGIAQRLDYLSWLGVDAIWISPFYTSPMKDFGYDIQDYRDVDPIFGTLA
DFDALAARARALGLKLILDFVPNHTSDRHPWFLESRQSPASAKRDWYIWRDPAADGGPPTNWLAEFGGSAWEYDEASGQY
YYHAFLKEQPDLNWRNPEVRAAMYDAMRFWLERGVDGFRVDVMWHLIKDDQFRDNPPNPARPPNAGPANALIPLYTADRP
EVHDIVREMRAVVDAYPERVLIGEIWLPTDRLVTYYGHDLRGAHLPFNFQLIGAAWDAGVIKEIVGDYEKALPRGGWPNW
VMGNHDKGRIASRLGPRQARLAMLLLLTLRGTPTLYYGDEIAMRDVPIPPDRVCDPYEKNVPGLGLGRDPQRTPMQWEDA
PQAGFTRASRSWLPIAADASTVNVAAQRAAPASMLTLVHRLIALRKAEAALTIGDYGALDAPERVVMFLRRVPGRRLLIA
LNLGESAQSLSLPGDSRQATTLLSTDPERPLGDLAPAFTLGGGEGIVVLLADRETAPPSPAA
>Mature_541_residues
SGQEWWKRAVIYQIYPRSFQDADGDGIGDLAGIAQRLDYLSWLGVDAIWISPFYTSPMKDFGYDIQDYRDVDPIFGTLAD
FDALAARARALGLKLILDFVPNHTSDRHPWFLESRQSPASAKRDWYIWRDPAADGGPPTNWLAEFGGSAWEYDEASGQYY
YHAFLKEQPDLNWRNPEVRAAMYDAMRFWLERGVDGFRVDVMWHLIKDDQFRDNPPNPARPPNAGPANALIPLYTADRPE
VHDIVREMRAVVDAYPERVLIGEIWLPTDRLVTYYGHDLRGAHLPFNFQLIGAAWDAGVIKEIVGDYEKALPRGGWPNWV
MGNHDKGRIASRLGPRQARLAMLLLLTLRGTPTLYYGDEIAMRDVPIPPDRVCDPYEKNVPGLGLGRDPQRTPMQWEDAP
QAGFTRASRSWLPIAADASTVNVAAQRAAPASMLTLVHRLIALRKAEAALTIGDYGALDAPERVVMFLRRVPGRRLLIAL
NLGESAQSLSLPGDSRQATTLLSTDPERPLGDLAPAFTLGGGEGIVVLLADRETAPPSPAA

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=498, Percent_Identity=39.9598393574297, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI65506891, Length=134, Percent_Identity=32.8358208955224, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI61744479, Length=134, Percent_Identity=32.8358208955224, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI61744475, Length=134, Percent_Identity=32.8358208955224, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI61744477, Length=134, Percent_Identity=32.8358208955224, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI61744481, Length=134, Percent_Identity=32.8358208955224, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI61744483, Length=134, Percent_Identity=32.8358208955224, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790687, Length=525, Percent_Identity=37.1428571428571, Blast_Score=311, Evalue=1e-85,
Organism=Escherichia coli, GI1786604, Length=494, Percent_Identity=26.5182186234818, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI87081873, Length=255, Percent_Identity=26.2745098039216, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI25147709, Length=537, Percent_Identity=29.7951582867784, Blast_Score=177, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI32565753, Length=507, Percent_Identity=26.0355029585799, Blast_Score=150, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322245, Length=587, Percent_Identity=31.8568994889268, Blast_Score=282, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6321726, Length=582, Percent_Identity=32.4742268041237, Blast_Score=275, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6322241, Length=582, Percent_Identity=32.8178694158076, Blast_Score=268, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6322021, Length=582, Percent_Identity=32.8178694158076, Blast_Score=268, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6324416, Length=592, Percent_Identity=32.2635135135135, Blast_Score=267, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6321731, Length=554, Percent_Identity=33.2129963898917, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6319776, Length=554, Percent_Identity=33.2129963898917, Blast_Score=261, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24586591, Length=556, Percent_Identity=40.2877697841727, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24586593, Length=513, Percent_Identity=41.9103313840156, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24586589, Length=520, Percent_Identity=41.5384615384615, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45549022, Length=519, Percent_Identity=40.2697495183044, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24586599, Length=527, Percent_Identity=40.0379506641366, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24583749, Length=520, Percent_Identity=39.0384615384615, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24583747, Length=520, Percent_Identity=39.0384615384615, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24586597, Length=500, Percent_Identity=42.4, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24583745, Length=557, Percent_Identity=37.8815080789946, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI221330053, Length=521, Percent_Identity=38.7715930902111, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24586587, Length=511, Percent_Identity=38.747553816047, Blast_Score=349, Evalue=3e-96,
Organism=Drosophila melanogaster, GI281360393, Length=459, Percent_Identity=39.6514161220044, Blast_Score=315, Evalue=6e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 60479; Mature: 60348

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGQEWWKRAVIYQIYPRSFQDADGDGIGDLAGIAQRLDYLSWLGVDAIWISPFYTSPMK
CCCHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHH
DFGYDIQDYRDVDPIFGTLADFDALAARARALGLKLILDFVPNHTSDRHPWFLESRQSPA
HHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCC
SAKRDWYIWRDPAADGGPPTNWLAEFGGSAWEYDEASGQYYYHAFLKEQPDLNWRNPEVR
CCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHH
AAMYDAMRFWLERGVDGFRVDVMWHLIKDDQFRDNPPNPARPPNAGPANALIPLYTADRP
HHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
EVHDIVREMRAVVDAYPERVLIGEIWLPTDRLVTYYGHDLRGAHLPFNFQLIGAAWDAGV
HHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHEECCCCCCCCCCCEEEEEEECCCCHHH
IKEIVGDYEKALPRGGWPNWVMGNHDKGRIASRLGPRQARLAMLLLLTLRGTPTLYYGDE
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCE
IAMRDVPIPPDRVCDPYEKNVPGLGLGRDPQRTPMQWEDAPQAGFTRASRSWLPIAADAS
EEECCCCCCCHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCEEECCCC
TVNVAAQRAAPASMLTLVHRLIALRKAEAALTIGDYGALDAPERVVMFLRRVPGRRLLIA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCEEEEE
LNLGESAQSLSLPGDSRQATTLLSTDPERPLGDLAPAFTLGGGEGIVVLLADRETAPPSP
EECCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCCEEECCCCEEEEEEECCCCCCCCC
AA
CC
>Mature Secondary Structure 
SGQEWWKRAVIYQIYPRSFQDADGDGIGDLAGIAQRLDYLSWLGVDAIWISPFYTSPMK
CCHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHH
DFGYDIQDYRDVDPIFGTLADFDALAARARALGLKLILDFVPNHTSDRHPWFLESRQSPA
HHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCC
SAKRDWYIWRDPAADGGPPTNWLAEFGGSAWEYDEASGQYYYHAFLKEQPDLNWRNPEVR
CCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHH
AAMYDAMRFWLERGVDGFRVDVMWHLIKDDQFRDNPPNPARPPNAGPANALIPLYTADRP
HHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
EVHDIVREMRAVVDAYPERVLIGEIWLPTDRLVTYYGHDLRGAHLPFNFQLIGAAWDAGV
HHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHEECCCCCCCCCCCEEEEEEECCCCHHH
IKEIVGDYEKALPRGGWPNWVMGNHDKGRIASRLGPRQARLAMLLLLTLRGTPTLYYGDE
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCE
IAMRDVPIPPDRVCDPYEKNVPGLGLGRDPQRTPMQWEDAPQAGFTRASRSWLPIAADAS
EEECCCCCCCHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCEEECCCC
TVNVAAQRAAPASMLTLVHRLIALRKAEAALTIGDYGALDAPERVVMFLRRVPGRRLLIA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCEEEEE
LNLGESAQSLSLPGDSRQATTLLSTDPERPLGDLAPAFTLGGGEGIVVLLADRETAPPSP
EECCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCCEEECCCCEEEEEEECCCCCCCCC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]