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The map label for this gene is nocM [H]

Identifier: 83593588

GI number: 83593588

Start: 2615209

End: 2615928

Strand: Direct

Name: nocM [H]

Synonym: Rru_A2253

Alternate gene names: 83593588

Gene position: 2615209-2615928 (Clockwise)

Preceding gene: 83593587

Following gene: 83593589

Centisome position: 60.08

GC content: 65.56

Gene sequence:

>720_bases
ATGAACCTGGATCTGGTCGCCACCTTCCTGCCCAAGCTGCTGTTCGAAGGGCTTCCCGTCACCCTCAAGCTGGTGACCCT
GGCCGTCGCCATCGGCGTTTTCATCGCCATCCCAACGGCGTTGCTGCGCGTTCACCCCAGCCCGCTGGTCCGCGCCTTCC
CTTACGCCTTCATCTTCTTTTTCCGCGGCACGCCGCTGCTGATCCAGATCGCCCTGGTCTATTACGGCCTGTCCACCCTG
CCGTGGATCCGTGAGAACGAGGTGCTGTGGCCGATCCTGCGCGAGCCGTTCTGGTGCGCGCTGATCGCTTTCGCCCTGAA
TACCGGCGCCTATTCGGCCGAGATCCTGCGCGGCGCCATCCAGGCCATTCCGCGCGGCGAGGTCGAGGCGGCGCGGGCCT
ATGGCATGCGCGGCCTGCTGATCGTGCGGCGGATCATCCTGCCGCGCGCCTTCCAGATCGGCCTGCCGGCCTATGGCAAC
GAGATCATCCTGATCCTCAAGGGCAGCGCCCTGGCCTCAAGCATCACCTTGCTCGATATCACCGGGGTCGCCCGCCTTCT
CTATGCCCGCTATTACACGCCGGTTGAGGCCTTCGTCACCGCCGGGGTGATCTATCTGATCCTGGTTTACCTGCTGACCC
GCGGCCTGCGGCTGATCGAACGCCGGCTGTCGCGCCACCTGCTCGGCCCGCCGGAAACCGAGCTTGCCGAAGACCGCTAG

Upstream 100 bases:

>100_bases
TCTGGCGATGACAATGGTCAGCATGGCCGTTATCGCCCTTCTTGAACGCCGGGCCAATCGCGGCCATGTCGGCGCGGCCG
GGCACTGAGGAACGCGATCG

Downstream 100 bases:

>100_bases
GGGCCGCTGACGGTGGCCGTCCCCCCTCCCCCCGCCCGGCTCGGCCGGCTCGAGCCCCTGGTCATCTATGGGCGACGACG
GGCGATGTGGGCCTATGGCA

Product: amino acid ABC transporter permease

Products: ADP; phosphate; L-histidine [Cytoplasm]; ADP; L-ornithine [Cytoplasm]; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNLDLVATFLPKLLFEGLPVTLKLVTLAVAIGVFIAIPTALLRVHPSPLVRAFPYAFIFFFRGTPLLIQIALVYYGLSTL
PWIRENEVLWPILREPFWCALIAFALNTGAYSAEILRGAIQAIPRGEVEAARAYGMRGLLIVRRIILPRAFQIGLPAYGN
EIILILKGSALASSITLLDITGVARLLYARYYTPVEAFVTAGVIYLILVYLLTRGLRLIERRLSRHLLGPPETELAEDR

Sequences:

>Translated_239_residues
MNLDLVATFLPKLLFEGLPVTLKLVTLAVAIGVFIAIPTALLRVHPSPLVRAFPYAFIFFFRGTPLLIQIALVYYGLSTL
PWIRENEVLWPILREPFWCALIAFALNTGAYSAEILRGAIQAIPRGEVEAARAYGMRGLLIVRRIILPRAFQIGLPAYGN
EIILILKGSALASSITLLDITGVARLLYARYYTPVEAFVTAGVIYLILVYLLTRGLRLIERRLSRHLLGPPETELAEDR
>Mature_239_residues
MNLDLVATFLPKLLFEGLPVTLKLVTLAVAIGVFIAIPTALLRVHPSPLVRAFPYAFIFFFRGTPLLIQIALVYYGLSTL
PWIRENEVLWPILREPFWCALIAFALNTGAYSAEILRGAIQAIPRGEVEAARAYGMRGLLIVRRIILPRAFQIGLPAYGN
EIILILKGSALASSITLLDITGVARLLYARYYTPVEAFVTAGVIYLILVYLLTRGLRLIERRLSRHLLGPPETELAEDR

Specific function: Component of the nopaline active transport system probably consisting of four subunits:Q, M, P and T. This system is also capable of transporting octopine provided that catabolic functions are induced with nopaline [H]

COG id: COG4160

COG function: function code E; ABC-type arginine/histidine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788645, Length=221, Percent_Identity=42.0814479638009, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787086, Length=214, Percent_Identity=39.7196261682243, Blast_Score=152, Evalue=1e-38,
Organism=Escherichia coli, GI1788226, Length=225, Percent_Identity=33.7777777777778, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1788646, Length=216, Percent_Identity=30.5555555555556, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787030, Length=213, Percent_Identity=31.924882629108, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787087, Length=131, Percent_Identity=32.824427480916, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1786873, Length=200, Percent_Identity=32.5, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI87082239, Length=214, Percent_Identity=29.9065420560748, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786874, Length=216, Percent_Identity=26.3888888888889, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI48994930, Length=124, Percent_Identity=29.0322580645161, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 26628; Mature: 26628

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDLVATFLPKLLFEGLPVTLKLVTLAVAIGVFIAIPTALLRVHPSPLVRAFPYAFIFF
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FRGTPLLIQIALVYYGLSTLPWIRENEVLWPILREPFWCALIAFALNTGAYSAEILRGAI
HCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHH
QAIPRGEVEAARAYGMRGLLIVRRIILPRAFQIGLPAYGNEIILILKGSALASSITLLDI
HHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHH
TGVARLLYARYYTPVEAFVTAGVIYLILVYLLTRGLRLIERRLSRHLLGPPETELAEDR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNLDLVATFLPKLLFEGLPVTLKLVTLAVAIGVFIAIPTALLRVHPSPLVRAFPYAFIFF
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FRGTPLLIQIALVYYGLSTLPWIRENEVLWPILREPFWCALIAFALNTGAYSAEILRGAI
HCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHH
QAIPRGEVEAARAYGMRGLLIVRRIILPRAFQIGLPAYGNEIILILKGSALASSITLLDI
HHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHH
TGVARLLYARYYTPVEAFVTAGVIYLILVYLLTRGLRLIERRLSRHLLGPPETELAEDR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O; ATP; L-ornithine [Periplasm]; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [P

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1732218; 11743193; 11743194 [H]