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The map label for this gene is graR [H]

Identifier: 82750361

GI number: 82750361

Start: 673568

End: 674242

Strand: Direct

Name: graR [H]

Synonym: SAB0608

Alternate gene names: 82750361

Gene position: 673568-674242 (Clockwise)

Preceding gene: 82750360

Following gene: 82750362

Centisome position: 24.56

GC content: 30.22

Gene sequence:

>675_bases
ATGCAAATACTACTAGTAGAAGATGACAATACTTTGTTTCAAGAATTGAAAAAAGAATTAGAACAATGGGATTTTAATGT
TGCTGGTATTGAAGATTTCGGCAAAGTAATGGATACATTTGAAAGTTTTAATCCTGAAATTGTTATATTGGATGTTCAAT
TACCTAAATATGATGGGTTTTATTGGTGCAGAAAAATGAGAGAAGTTTCCAACGTACCAATATTATTTTTATCATCTCGT
GATAATCCAATGGATCAAGTGATGAGTATGGAACTTGGCGCAGATGATTATATGCAAAAGCCTTTTTATACAAATGTATT
AATTGCTAAATTACAAGCGATTTATCGACGAGTGTATGAATTTACTGCTGAAGAAAAACGCACGTTAACATGGCAAGATG
CCATTATAGATCTATCAAAAGATAGTATACAAAAAGGTGACGATACGATTTTTCTATCCAAAACAGAAATGATTATATTA
GAAATACTTATTACCAAAAAAAATCAAATCGTTTCGAGAGATACAATTATCACTGCATTATGGGATGATGAAGCATTTGT
TAGTGATAATACGTTAACAGTAAATGTGAATCGTTTACGAAAAAAATTATCTGAAATAAGTATGGATAGTGCAATCGAAA
CAAAAGTAGGAAAAGGATATATGGCTCATGAATAA

Upstream 100 bases:

>100_bases
GATTCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACTA
AATGATATTGGGTGATATGG

Downstream 100 bases:

>100_bases
TTTGAAATGGGTAGCTTATTTTTTGAAATCTCGCATGAACTGGATATTTTGGATATTGTTTTTAAACTTGCTTATGTTAG
GCATTAGTCTAATCGATTAT

Product: two-component response regulator

Products: NA

Alternate protein names: Glycopeptide resistance-associated protein R [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFNPEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSR
DNPMDQVMSMELGADDYMQKPFYTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAIIDLSKDSIQKGDDTIFLSKTEMIIL
EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGKGYMAHE

Sequences:

>Translated_224_residues
MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFNPEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSR
DNPMDQVMSMELGADDYMQKPFYTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAIIDLSKDSIQKGDDTIFLSKTEMIIL
EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGKGYMAHE
>Mature_224_residues
MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFNPEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSR
DNPMDQVMSMELGADDYMQKPFYTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAIIDLSKDSIQKGDDTIFLSKTEMIIL
EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGKGYMAHE

Specific function: Member of the two-component regulatory system graR/graS involved in resistance against cationic antimicrobial peptides (CAMPs) [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=222, Percent_Identity=28.3783783783784, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI1787375, Length=222, Percent_Identity=31.5315315315315, Blast_Score=113, Evalue=9e-27,
Organism=Escherichia coli, GI1790860, Length=222, Percent_Identity=30.6306306306306, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=29.4642857142857, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1786911, Length=219, Percent_Identity=27.8538812785388, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=28.3842794759825, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1789402, Length=227, Percent_Identity=32.15859030837, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790863, Length=229, Percent_Identity=27.5109170305677, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI2367329, Length=226, Percent_Identity=28.7610619469027, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=28.7610619469027, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI87082012, Length=220, Percent_Identity=26.3636363636364, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1787229, Length=224, Percent_Identity=24.1071428571429, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI145693140, Length=230, Percent_Identity=29.1304347826087, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26094; Mature: 26094

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFNPEIVILDVQLPKYDGF
CEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCH
YWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPFYTNVLIAKLQAIYRRVYE
HHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
FTAEEKRTLTWQDAIIDLSKDSIQKGDDTIFLSKTEMIILEILITKKNQIVSRDTIITAL
HHHHHCCEEEHHHHEEECCHHHCCCCCCEEEEECHHEEEEEEEHHCCCCEEECCEEEEEE
WDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGKGYMAHE
ECCCEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFNPEIVILDVQLPKYDGF
CEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCH
YWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPFYTNVLIAKLQAIYRRVYE
HHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
FTAEEKRTLTWQDAIIDLSKDSIQKGDDTIFLSKTEMIILEILITKKNQIVSRDTIITAL
HHHHHCCEEEHHHHEEECCHHHCCCCCCEEEEECHHEEEEEEEHHCCCCEEECCEEEEEE
WDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGKGYMAHE
ECCCEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA