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The map label for this gene is graS [H]

Identifier: 82750362

GI number: 82750362

Start: 674235

End: 675275

Strand: Direct

Name: graS [H]

Synonym: SAB0609

Alternate gene names: 82750362

Gene position: 674235-675275 (Clockwise)

Preceding gene: 82750361

Following gene: 82750363

Centisome position: 24.58

GC content: 30.36

Gene sequence:

>1041_bases
ATGAATAATTTGAAATGGGTAGCTTATTTTTTGAAATCTCGCATGAACTGGATATTTTGGATATTGTTTTTAAACTTGCT
TATGTTAGGCATTAGTCTAATCGATTATGATTTTCCAATAGACAGTTTATTTTATATTGTTTCTTTGAATTTAAGTTTAA
CAATGATTTTTCTTATATTGACATATTTTAAAGAAGTAAAATTATATAAGCATTTTGACAAAGATAAAGAAATAGAAGAA
ATTAAACATAAAGATTTTGCGGAAACGCCATTTCAACGTCATACAGTTGATTATTTATATCGTCAAATCTCAGCTCACAA
AGAAAAGGTTGTTGAGCAACAGTTACAATTGAATATGCATGAACAGACCATTACAGAATTTGTGCACGACATAAAAACAC
CTGTGACAGCCATGAAATTATTAATTGATCAAGAAAAAAATCAAGAAAGAAAACAGGCATTACTATATGAATGGTCTCGT
ATAAACTCGATGCTGGATACACAGCTGTATATTACTAGATTAGAATCTCAACGTAAAGATATGTATTTTGATTACGTGTC
ACTTAAACGCATGGTCATTGATGAAATACAATTAACAAGACATATTAGTCAGGTTAAAGGTATTGGTTTTGATGTTGACT
TTAAAGTGGATGATTATGTTTATACAGATATAAAATGGTGTCGTATGATTATTAGACAAATTTTGTCAAACGCATTGAAA
TATAGTGAGAATTTTAATATTGAAATTGGGACAGAATTAAATGATCAACATGTTTCGTTATATATTAAAGACTATGGCAG
AGGTATTAGTAAAAAAGATATGCCGCGAATATTTGAACGAGGATTTACGTCAACGGCTAACAGAAATGAAACGACGTCTT
CAGGTATGGGTCTATATTTAGTAAATAGTGTAAAGGATCAATTAGGTATTCACCTGCAAGTCACGTCGACTGTTGGTAAG
GGGACAACTGTCAGATTGATTTTCCCATTACAAAATGAAATTGTTGAACGCATGTCGGAAGTGACAAATTTGTCATTTTA
A

Upstream 100 bases:

>100_bases
AATACGTTAACAGTAAATGTGAATCGTTTACGAAAAAAATTATCTGAAATAAGTATGGATAGTGCAATCGAAACAAAAGT
AGGAAAAGGATATATGGCTC

Downstream 100 bases:

>100_bases
ACATGCGTTTTGTTACTTAGAATTGATACATCAATGCAGCTTCAACGTTATAATAAGATAGATGTTAGTCATATGTTAAA
TTGAAGATACAAGTGCCAAA

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: Glycopeptide resistance-associated protein S [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLFYIVSLNLSLTMIFLILTYFKEVKLYKHFDKDKEIEE
IKHKDFAETPFQRHTVDYLYRQISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDDYVYTDIKWCRMIIRQILSNALK
YSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPRIFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGK
GTTVRLIFPLQNEIVERMSEVTNLSF

Sequences:

>Translated_346_residues
MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLFYIVSLNLSLTMIFLILTYFKEVKLYKHFDKDKEIEE
IKHKDFAETPFQRHTVDYLYRQISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDDYVYTDIKWCRMIIRQILSNALK
YSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPRIFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGK
GTTVRLIFPLQNEIVERMSEVTNLSF
>Mature_346_residues
MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLFYIVSLNLSLTMIFLILTYFKEVKLYKHFDKDKEIEE
IKHKDFAETPFQRHTVDYLYRQISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDDYVYTDIKWCRMIIRQILSNALK
YSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPRIFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGK
GTTVRLIFPLQNEIVERMSEVTNLSF

Specific function: Member of the two-component regulatory system graR/graS involved in resistance against cationic antimicrobial peptides (CAMPs). GraS probably functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phospha

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=224, Percent_Identity=20.5357142857143, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788713, Length=235, Percent_Identity=24.2553191489362, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41079; Mature: 41079

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLFYIVSLNLSLTMIFLIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
TYFKEVKLYKHFDKDKEIEEIKHKDFAETPFQRHTVDYLYRQISAHKEKVVEQQLQLNMH
HHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
EQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSRINSMLDTQLYITRLESQRKD
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDDYVYTDIKWCRMIIRQILSNALK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEHHHHHHHHHHHHHHHHHHH
YSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPRIFERGFTSTANRNETTSSGMGLYL
CCCCCCEEECCCCCCCCEEEEEEHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEE
VNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEIVERMSEVTNLSF
HHHHHHHHCEEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLFYIVSLNLSLTMIFLIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
TYFKEVKLYKHFDKDKEIEEIKHKDFAETPFQRHTVDYLYRQISAHKEKVVEQQLQLNMH
HHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
EQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSRINSMLDTQLYITRLESQRKD
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDDYVYTDIKWCRMIIRQILSNALK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEHHHHHHHHHHHHHHHHHHH
YSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPRIFERGFTSTANRNETTSSGMGLYL
CCCCCCEEECCCCCCCCEEEEEEHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEE
VNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEIVERMSEVTNLSF
HHHHHHHHCEEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA