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The map label for this gene is vraB [H]

Identifier: 82750281

GI number: 82750281

Start: 592701

End: 593840

Strand: Direct

Name: vraB [H]

Synonym: SAB0526

Alternate gene names: 82750281

Gene position: 592701-593840 (Clockwise)

Preceding gene: 82750280

Following gene: 82750282

Centisome position: 21.61

GC content: 38.25

Gene sequence:

>1140_bases
ATGAATCAAGCAGTCATAGTTGCAGCTAAACGAACTGCATTTGGGAAATATGGAGGCACTTTAAAACATATAGAGCCAGA
ACAATTGCTCAAACCTTTATTCCAACATTTTAAAGAGAAGTATCCAGAAGTAATATCTAAAATAGATGATGTAGTTTTAG
GTAATGTTGTTGGGAATGGTGGCAATATTGCAAGAAAAGCATTGCTTGAAGCGGGGCTTAAAGATTCAATACCTGGCGTC
ACAATCGATCGGCAATGTGGGTCTGGACTTGAAAGTGTTCAATATTCATGTCGCATGATTCAAGCCGGAGCTGGCAAGGT
ATATATTGCAGGCGGTGTTGAAAGTACAAGTCGAGCACCTTGGAAAATCAAACGACCGCATTCTGTGTACGAAACAGCAT
TACCTGAGTTTTATGAGCGTGCATCATTTGCACCTGAAATGAGCGACCCATCAATGATTCAAGGTGCTGAAAATGCAGCC
AAGATGTATGATGTTTCAAGAGAATTACAAGATGAATTTGCTTATCGAAGTCATCAATTGACAGCAGAAAATGTAAAGAA
TGGAAATATTTCTCAGGAAATATTACCTATAACCGTTAAAGGAGAAATATTCAACACTGATGAAAGTCTAAAATCACATA
TTCCGAAAGATAACTTTGGCCGATTTAAGCCCGTGATCAAAGGTGGGACCGTTACCGCTGCGAATAGTTGTATGAAAAAT
GATGGTGCAGTTTTATTGCTTATTATGGAAAAAGATATGGCATACGAATTAGATTTCGAGCATGGTTTATTATTTAAAGA
TGGTGTTACGGTAGGTGTTGATTCTAATTTTCCTGGCATTGGTCCAGTACCAGCCATTTCCAACTTACTAAAAAGAAATC
AATTAACGATAGAAAATATTGAAGTCATTGAAATTAACGAAGCGTTCAGTGCACAGGTGGTTGCCTGCCAACAAGCTTTA
AATATTTCAAATACGCAATTAAATATATGGGGTGGTGCATTAGCATCAGGTCATCCATACGGTGCAAGCGGTGCCCAATT
AGTGACGCGATTATTTTATATGTTTGACAAAGAGACTATGATTGCATCTATGGGGATTGGGGGAGGTCTAGGAAATGCAG
CATTATTTACTCGATTCTAA

Upstream 100 bases:

>100_bases
CCATCGATGATTCATCATGTAGAAAAGATGTATTACACTGCAAGTGGTAAAATTGCTAGAGAAAAAATGATATCGATGTA
TTTGAGAGGTGAATTATAAT

Downstream 100 bases:

>100_bases
CCAGCGATTAAATGTGTCATTTTCTAAGGATAGTGTGGCTGCATATTATCAGTGTTTTAACCAACCTTATAGAAAAGAAG
TACCACCATTAATGTGTGCG

Product: acetyl-CoA c-acetyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MNQAVIVAAKRTAFGKYGGTLKHIEPEQLLKPLFQHFKEKYPEVISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGV
TIDRQCGSGLESVQYSCRMIQAGAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENAA
KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDNFGRFKPVIKGGTVTAANSCMKN
DGAVLLLIMEKDMAYELDFEHGLLFKDGVTVGVDSNFPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQAL
NISNTQLNIWGGALASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF

Sequences:

>Translated_379_residues
MNQAVIVAAKRTAFGKYGGTLKHIEPEQLLKPLFQHFKEKYPEVISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGV
TIDRQCGSGLESVQYSCRMIQAGAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENAA
KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDNFGRFKPVIKGGTVTAANSCMKN
DGAVLLLIMEKDMAYELDFEHGLLFKDGVTVGVDSNFPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQAL
NISNTQLNIWGGALASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF
>Mature_379_residues
MNQAVIVAAKRTAFGKYGGTLKHIEPEQLLKPLFQHFKEKYPEVISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGV
TIDRQCGSGLESVQYSCRMIQAGAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENAA
KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDNFGRFKPVIKGGTVTAANSCMKN
DGAVLLLIMEKDMAYELDFEHGLLFKDGVTVGVDSNFPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQAL
NISNTQLNIWGGALASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=392, Percent_Identity=31.8877551020408, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI148539872, Length=388, Percent_Identity=31.9587628865979, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI4501853, Length=389, Percent_Identity=31.3624678663239, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI4557237, Length=397, Percent_Identity=33.2493702770781, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4504327, Length=413, Percent_Identity=27.8450363196126, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1787663, Length=400, Percent_Identity=35, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788554, Length=393, Percent_Identity=35.8778625954198, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI48994986, Length=396, Percent_Identity=33.0808080808081, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI87082165, Length=391, Percent_Identity=31.2020460358056, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788683, Length=413, Percent_Identity=24.6973365617433, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI133906874, Length=389, Percent_Identity=34.7043701799486, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI25147385, Length=388, Percent_Identity=29.3814432989691, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=30.0236406619385, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17535921, Length=397, Percent_Identity=27.2040302267003, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17535917, Length=368, Percent_Identity=25.8152173913043, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322031, Length=385, Percent_Identity=33.7662337662338, Blast_Score=182, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6325229, Length=392, Percent_Identity=29.3367346938776, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24655093, Length=391, Percent_Identity=31.4578005115089, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI17648125, Length=388, Percent_Identity=34.2783505154639, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24640423, Length=390, Percent_Identity=31.5384615384615, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=29.5081967213115, Blast_Score=144, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 41141; Mature: 41141

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQAVIVAAKRTAFGKYGGTLKHIEPEQLLKPLFQHFKEKYPEVISKIDDVVLGNVVGNG
CCCEEEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
GNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYSCRMIQAGAGKVYIAGGVESTSRAP
CHHHHHHHHHCCCCCCCCCCEEEHHHCCCHHHHHHHHHHEECCCCEEEEECCCCCCCCCC
WKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENAAKMYDVSRELQDEFAYRSHQL
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
TAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDNFGRFKPVIKGGTVTAANSCMKN
CHHHCCCCCCCCEEEEEEEECEEECCCHHHHHCCCCCCCCCCCCEECCCCEEHHHHHHCC
DGAVLLLIMEKDMAYELDFEHGLLFKDGVTVGVDSNFPGIGPVPAISNLLKRNQLTIENI
CCCEEEEEEECCCEEEEECCCCEEEECCEEEEECCCCCCCCCHHHHHHHHHHCCCEECCE
EVIEINEAFSAQVVACQQALNISNTQLNIWGGALASGHPYGASGAQLVTRLFYMFDKETM
EEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCHHH
IASMGIGGGLGNAALFTRF
EEECCCCCCCCCCCCEECC
>Mature Secondary Structure
MNQAVIVAAKRTAFGKYGGTLKHIEPEQLLKPLFQHFKEKYPEVISKIDDVVLGNVVGNG
CCCEEEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
GNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYSCRMIQAGAGKVYIAGGVESTSRAP
CHHHHHHHHHCCCCCCCCCCEEEHHHCCCHHHHHHHHHHEECCCCEEEEECCCCCCCCCC
WKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENAAKMYDVSRELQDEFAYRSHQL
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
TAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDNFGRFKPVIKGGTVTAANSCMKN
CHHHCCCCCCCCEEEEEEEECEEECCCHHHHHCCCCCCCCCCCCEECCCCEEHHHHHHCC
DGAVLLLIMEKDMAYELDFEHGLLFKDGVTVGVDSNFPGIGPVPAISNLLKRNQLTIENI
CCCEEEEEEECCCEEEEECCCCEEEECCEEEEECCCCCCCCCHHHHHHHHHHCCCEECCE
EVIEINEAFSAQVVACQQALNISNTQLNIWGGALASGHPYGASGAQLVTRLFYMFDKETM
EEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCHHH
IASMGIGGGLGNAALFTRF
EEECCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]