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The map label for this gene is vraA [H]

Identifier: 82750280

GI number: 82750280

Start: 591323

End: 592699

Strand: Direct

Name: vraA [H]

Synonym: SAB0525

Alternate gene names: 82750280

Gene position: 591323-592699 (Clockwise)

Preceding gene: 82750279

Following gene: 82750281

Centisome position: 21.56

GC content: 32.03

Gene sequence:

>1377_bases
ATGAACGTAATTTTAGAACAGTTGAAAATACATACTCAAAATAAACCTAATGACATAGCATTACATATAGATGATGAAAT
AATTACATATAGTCAACTAAATGCCCGCATCACTAGCGCAGTTGAATCTTTGCAGAAATATTCACTTAACCCTGTCGTTG
CTATTAATATGAAATCACCGGTTCAAAGTATTATTTGTTATTTAGCTTTGCATCGTTTACATAAAGTGCCTATGATGATG
GAAGGTAAATGGCAAAGTACTATACATCGTCAATTGATTGAGAAATATGGTATTAAAGATGTAATTGAAGATACAGGTCT
CATGCAGAGTATAGACTCACCGATGTTTATTGATTCAACGCAATTACAGCACTACCCCAATTTATTACATATTGGTTTTA
CTTCAGGGACAACTGGACTGCCAAAAGCATATTATCGTGATGAAGATTCATGGTTGGCTTCTTTTGAAGTTAATGAAATG
TTGATGTTAAAAAATGAAAATGCCATAGCAGCCCCCGGACCACTATCGTACTCGTTAACATTATATGCGTTATTGTTTGC
TTTAAGTTCTGGTCGTACTTTTATAGGACAGACCACTTTTCATCCTGAAAAGTTACTTAATCAATGTCGTAAAATATCAT
CATACAAAGTTGCTATGTTTCTTGTTCCAACGATGATTAAATCATTATTGTTAGTTTACAACAATGAACATACAATCCAA
TCATTTTTTAGCAGTGGAGATAAGCTGCATTCTTCTATTTTTAAAAAGATAAAAAATCAAGCAAATGACATAAATTTGAT
TGAATTTTTTGGTACATCGGAAACCAGTTTTATCAGCTATAACTTGAATCAGCAAGCACCAGTTGAATCAGTAGGTGTGC
TATTTCCAAATGTGGAATTGAAAACAACGAATCACGATCACAATGGTATAGGAACTATTTGTATAAAAAGTAATATGATG
TTTAGTGGCTATGTAAGCGAACAATGTATAAATAATGATGAATGGTTTGTTACTAATGATAATGGCTATGTAAAAGAGCA
GTATTTATATTTAACGGGACGACAACATGATATGTTAATTATTGGTGGTCAAAATATATATCCAGCACATGTTGAACGCC
TTTTAACGCAATCGTCGAGCATTGATGAAGCAATTATAATCGGTATTCCAAATAAGCGTTTTGGTCAAATAGGTGTATTG
CTTTATTTTGGTGATGTGACACTTACACATAAAAATGTAAAACAATTTTTAAATAAGAAAGTGAAACGCTATGAAATTCC
ATCGATGATTCATCATGTAGAAAAGATGTATTACACTGCAAGTGGTAAAATTGCTAGAGAAAAAATGATATCGATGTATT
TGAGAGGTGAATTATAA

Upstream 100 bases:

>100_bases
TTGATTTTTATCATAGGTAGTATGACCTGATAAAAATGCTTGTTCATTATATAATGTTAACAAGATGTATTTTAAAGTTT
ACATTGAGTGAGGGATATTG

Downstream 100 bases:

>100_bases
TATGAATCAAGCAGTCATAGTTGCAGCTAAACGAACTGCATTTGGGAAATATGGAGGCACTTTAAAACATATAGAGCCAG
AACAATTGCTCAAACCTTTA

Product: long chain fatty acid CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MNVILEQLKIHTQNKPNDIALHIDDEIITYSQLNARITSAVESLQKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMM
EGKWQSTIHRQLIEKYGIKDVIEDTGLMQSIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
LMLKNENAIAAPGPLSYSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCRKISSYKVAMFLVPTMIKSLLLVYNNEHTIQ
SFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETSFISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMM
FSGYVSEQCINNDEWFVTNDNGYVKEQYLYLTGRQHDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNKRFGQIGVL
LYFGDVTLTHKNVKQFLNKKVKRYEIPSMIHHVEKMYYTASGKIAREKMISMYLRGEL

Sequences:

>Translated_458_residues
MNVILEQLKIHTQNKPNDIALHIDDEIITYSQLNARITSAVESLQKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMM
EGKWQSTIHRQLIEKYGIKDVIEDTGLMQSIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
LMLKNENAIAAPGPLSYSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCRKISSYKVAMFLVPTMIKSLLLVYNNEHTIQ
SFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETSFISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMM
FSGYVSEQCINNDEWFVTNDNGYVKEQYLYLTGRQHDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNKRFGQIGVL
LYFGDVTLTHKNVKQFLNKKVKRYEIPSMIHHVEKMYYTASGKIAREKMISMYLRGEL
>Mature_458_residues
MNVILEQLKIHTQNKPNDIALHIDDEIITYSQLNARITSAVESLQKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMM
EGKWQSTIHRQLIEKYGIKDVIEDTGLMQSIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
LMLKNENAIAAPGPLSYSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCRKISSYKVAMFLVPTMIKSLLLVYNNEHTIQ
SFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETSFISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMM
FSGYVSEQCINNDEWFVTNDNGYVKEQYLYLTGRQHDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNKRFGQIGVL
LYFGDVTLTHKNVKQFLNKKVKRYEIPSMIHHVEKMYYTASGKIAREKMISMYLRGEL

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=354, Percent_Identity=26.8361581920904, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1788107, Length=392, Percent_Identity=22.9591836734694, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693145, Length=352, Percent_Identity=26.1363636363636, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788595, Length=333, Percent_Identity=23.1231231231231, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71994703, Length=355, Percent_Identity=24.5070422535211, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71994694, Length=355, Percent_Identity=24.7887323943662, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71994690, Length=360, Percent_Identity=25, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI32563687, Length=319, Percent_Identity=27.5862068965517, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17558820, Length=355, Percent_Identity=25.9154929577465, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=361, Percent_Identity=24.6537396121884, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17531443, Length=357, Percent_Identity=23.249299719888, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=350, Percent_Identity=22.2857142857143, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17557194, Length=396, Percent_Identity=22.2222222222222, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17538037, Length=390, Percent_Identity=21.025641025641, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71996755, Length=339, Percent_Identity=21.2389380530973, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=156, Percent_Identity=28.2051282051282, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24581924, Length=361, Percent_Identity=24.6537396121884, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356947, Length=392, Percent_Identity=21.9387755102041, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24648257, Length=343, Percent_Identity=25.3644314868805, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24648260, Length=367, Percent_Identity=24.2506811989101, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17933690, Length=135, Percent_Identity=31.8518518518519, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922652, Length=316, Percent_Identity=23.7341772151899, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24656500, Length=345, Percent_Identity=23.768115942029, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355181, Length=430, Percent_Identity=23.2558139534884, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21358303, Length=287, Percent_Identity=25.7839721254355, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281365686, Length=284, Percent_Identity=25.3521126760563, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 52175; Mature: 52175

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVILEQLKIHTQNKPNDIALHIDDEIITYSQLNARITSAVESLQKYSLNPVVAINMKSP
CCCHHHHHHHHCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
VQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVIEDTGLMQSIDSPMFIDST
HHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECH
QLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEMLMLKNENAIAAPGPLSYSLT
HHHHCCCEEEEEECCCCCCCCHHHCCCCCCEEEEEECCEEEEEECCCCEECCCCCCHHHH
LYALLFALSSGRTFIGQTTFHPEKLLNQCRKISSYKVAMFLVPTMIKSLLLVYNNEHTIQ
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHH
SFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETSFISYNLNQQAPVESVGVLFPNVEL
HHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHCEEECCEEE
KTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDEWFVTNDNGYVKEQYLYLTGRQHDMLI
EECCCCCCCEEEEEEECCEEEECCHHHHHCCCCCEEEECCCCEEEEEEEEEECCCCCEEE
IGGQNIYPAHVERLLTQSSSIDEAIIIGIPNKRFGQIGVLLYFGDVTLTHKNVKQFLNKK
ECCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEECCEEEEHHHHHHHHHHH
VKRYEIPSMIHHVEKMYYTASGKIAREKMISMYLRGEL
HHHHCHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNVILEQLKIHTQNKPNDIALHIDDEIITYSQLNARITSAVESLQKYSLNPVVAINMKSP
CCCHHHHHHHHCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
VQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVIEDTGLMQSIDSPMFIDST
HHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECH
QLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEMLMLKNENAIAAPGPLSYSLT
HHHHCCCEEEEEECCCCCCCCHHHCCCCCCEEEEEECCEEEEEECCCCEECCCCCCHHHH
LYALLFALSSGRTFIGQTTFHPEKLLNQCRKISSYKVAMFLVPTMIKSLLLVYNNEHTIQ
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHH
SFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETSFISYNLNQQAPVESVGVLFPNVEL
HHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHCEEECCEEE
KTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDEWFVTNDNGYVKEQYLYLTGRQHDMLI
EECCCCCCCEEEEEEECCEEEECCHHHHHCCCCCEEEECCCCEEEEEEEEEECCCCCEEE
IGGQNIYPAHVERLLTQSSSIDEAIIIGIPNKRFGQIGVLLYFGDVTLTHKNVKQFLNKK
ECCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEECCEEEEHHHHHHHHHHH
VKRYEIPSMIHHVEKMYYTASGKIAREKMISMYLRGEL
HHHHCHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]