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The map label for this gene is aphA [H]

Identifier: 82703416

GI number: 82703416

Start: 2615085

End: 2617097

Strand: Reverse

Name: aphA [H]

Synonym: Nmul_A2299

Alternate gene names: 82703416

Gene position: 2617097-2615085 (Counterclockwise)

Preceding gene: 82703420

Following gene: 82703415

Centisome position: 82.19

GC content: 53.55

Gene sequence:

>2013_bases
ATGATATCCCTAAACCAAAACTACACAGGGAGTGTTGCGTGCGCTTCTGAGAACGCTCGCGATATGACCCGTCTTACCAC
TGTGGAGAATGTTCGTACCCCGATTGCAGAAATTCTCGATCAGAGTCTGAACGAAATTTATCTGCTGAATGCTCAGACAC
TGCATTTCGAGTACGCCAGCCGGGGAGCCCGGCGCAATCTTGGCTACAGCATGGATTCGCTGCAAAAGATGACGCTGCTG
GAAGTCGAACGCGAGTTCGACGAGACATCGTTCCGGGCTTTGATTGATCCACTCATGAAGGGTGGGAAAGAAATCCTGGT
GTTCGAGGCGACCCATCTGCGCGCGGACGGTACGCATTATCCGGTCGAGGTTCACCTGGAGCTGTCCACCCGCCCCGAGC
AAGTGCAATTTCTTGTAATGGCGTTCGATCTCACCGCCCGCCGCCGCGCAGAGGCGAAGTTGCGTGCAAATGAGGCGCGC
TTCCGGACAATGGTCAATACTATCCCGCAGCTTGCATGGATAGGGGAGACGGACGGCTCCAGATCCTGGTACAACCAGCG
CTGGTACGACTATACCGGCACCACTCCCGAAGAGATGATGGGCTGGGGCTGGCAAAAGGTTCATGATCCCCAGCTGCTGC
CGGAGGTAATAAAACGTTGGAGCGAAGCTACTGCTGCTGAGCAGCCGCTCGATATGGAAATTCCACTGCGGGGTGCAGAC
GGGGAACTCCGGGTATTTCTGACCCGCGCCGAGCCGTTAAGAAACGAGGACGGTCAGGTTGTGCAGTGGTTCGGGACCAA
TACCGACGTCCACGATCAGAGACTCCGGGAAGAGGCGCAGCGTGAGACAGAAAAGCGGCTTCAGGTGGTGATGGAGCATA
TGAGCGAGGGGCTTATCATCTCCGATTTCAATCGTAATCTTCTCCGCTGGAATCCTGCCGCTTTGCGAATGCATGAGATC
GAGAATGCGGAAGAAGTTTTTGGCAGTCTTCATAATTTTGCCCGGATCTATGAGTTATCGACAACCGAGGGCAACGTCGT
GCCTATAGATCAATGGCCTTTGACGCGTATTCTCCGGGGCGAACACCTGCATGATCTGGAACTGCGCATCAGGCGGCTGG
ATAAAGAGTGGTTGAGAATCTTCAGTTATTCCGGAACGGTTTTGCGCTACGAGAACAGTAAAGGGCTGGCTTTTCTTACC
ATTAAGGATATCACCGAGCGCAAACAGGCGGAGGAAGCCTTGCGTGACGCCAAGGTTAATCTTGAATACAAGGTGAATGA
GCGTACTGCCCAGCTTCTTGCCAAGAGCAAGGAACTGGAAAATTTCTGTTATTCGGTATCGCATGATCTGAGGGCGCCCC
TGCGTGGGATAGCCGGGTACAGCCGGCTGTTGCTGGAAAACTACTACGAACGCCTCGACGAGGAAGGTCGCAGTTTTCTG
ACAAATGTCCGTTCAGCCACCAAGCATATGACGGATTTAATCGAAGATCTGCTCTCCTACTCGAAACTTGAGCGGCGAAA
ACTGTCTTCGATGGTCATCAGGCTTCCCGCTTTCGTATCAAAGGTGTTGGCCCAGTTTGGGGATAATCTGCAGAACGTGC
GCCTCACTGTCAGTATGGATGATTTCCACGTTCGCGCTGATCCCGATGGTCTTGCGATCGCTCTACGCAACCTGGTCGAT
AACGCTGTCAAGTTCTCCATCCATGCTCCAGAGCCCACCATCGAAATCCGTGCCTGGACTTCGGATAGCAATTGCATTCT
TTGCGTGCGCGACAACGGTATTGGATTCGATATGCGTTTTTACGACAAAATTTTTGAGATATTCCAGCGCTTGCAACGGG
CCGAGGAATATCCGGGGACGGGCATCGGGCTTGCAATGGTGCAAAAAGCGATGGAGCGTATGGGAGGACGGGTGTGGGCG
GAAAGCCAGGTAGGTGCCGGTGCTGTCTTTTACCTGCAACTGCCACTGTCTGGCCTTATGCCATTCGAGCAGTTGCAGGA
ACAGGCCTTATAG

Upstream 100 bases:

>100_bases
AGCTATTCACCCGGCCCGTTTTAGGGTTTCATAGCTCTGTTTTTTCCAAGTTTTTTCCGAGTCGAATCGTTCTTGCAAAA
GAGCATCAGGTTTTTCCGTA

Downstream 100 bases:

>100_bases
GGGTGTGTTTTCATGAACACTGCGTTGCCGATTCTGCTTATTGAAGACAACCCGATGGATGTCGATCTCACGCGGCGTGC
TTTTGTGCGGCACAACCTGA

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MISLNQNYTGSVACASENARDMTRLTTVENVRTPIAEILDQSLNEIYLLNAQTLHFEYASRGARRNLGYSMDSLQKMTLL
EVEREFDETSFRALIDPLMKGGKEILVFEATHLRADGTHYPVEVHLELSTRPEQVQFLVMAFDLTARRRAEAKLRANEAR
FRTMVNTIPQLAWIGETDGSRSWYNQRWYDYTGTTPEEMMGWGWQKVHDPQLLPEVIKRWSEATAAEQPLDMEIPLRGAD
GELRVFLTRAEPLRNEDGQVVQWFGTNTDVHDQRLREEAQRETEKRLQVVMEHMSEGLIISDFNRNLLRWNPAALRMHEI
ENAEEVFGSLHNFARIYELSTTEGNVVPIDQWPLTRILRGEHLHDLELRIRRLDKEWLRIFSYSGTVLRYENSKGLAFLT
IKDITERKQAEEALRDAKVNLEYKVNERTAQLLAKSKELENFCYSVSHDLRAPLRGIAGYSRLLLENYYERLDEEGRSFL
TNVRSATKHMTDLIEDLLSYSKLERRKLSSMVIRLPAFVSKVLAQFGDNLQNVRLTVSMDDFHVRADPDGLAIALRNLVD
NAVKFSIHAPEPTIEIRAWTSDSNCILCVRDNGIGFDMRFYDKIFEIFQRLQRAEEYPGTGIGLAMVQKAMERMGGRVWA
ESQVGAGAVFYLQLPLSGLMPFEQLQEQAL

Sequences:

>Translated_670_residues
MISLNQNYTGSVACASENARDMTRLTTVENVRTPIAEILDQSLNEIYLLNAQTLHFEYASRGARRNLGYSMDSLQKMTLL
EVEREFDETSFRALIDPLMKGGKEILVFEATHLRADGTHYPVEVHLELSTRPEQVQFLVMAFDLTARRRAEAKLRANEAR
FRTMVNTIPQLAWIGETDGSRSWYNQRWYDYTGTTPEEMMGWGWQKVHDPQLLPEVIKRWSEATAAEQPLDMEIPLRGAD
GELRVFLTRAEPLRNEDGQVVQWFGTNTDVHDQRLREEAQRETEKRLQVVMEHMSEGLIISDFNRNLLRWNPAALRMHEI
ENAEEVFGSLHNFARIYELSTTEGNVVPIDQWPLTRILRGEHLHDLELRIRRLDKEWLRIFSYSGTVLRYENSKGLAFLT
IKDITERKQAEEALRDAKVNLEYKVNERTAQLLAKSKELENFCYSVSHDLRAPLRGIAGYSRLLLENYYERLDEEGRSFL
TNVRSATKHMTDLIEDLLSYSKLERRKLSSMVIRLPAFVSKVLAQFGDNLQNVRLTVSMDDFHVRADPDGLAIALRNLVD
NAVKFSIHAPEPTIEIRAWTSDSNCILCVRDNGIGFDMRFYDKIFEIFQRLQRAEEYPGTGIGLAMVQKAMERMGGRVWA
ESQVGAGAVFYLQLPLSGLMPFEQLQEQAL
>Mature_670_residues
MISLNQNYTGSVACASENARDMTRLTTVENVRTPIAEILDQSLNEIYLLNAQTLHFEYASRGARRNLGYSMDSLQKMTLL
EVEREFDETSFRALIDPLMKGGKEILVFEATHLRADGTHYPVEVHLELSTRPEQVQFLVMAFDLTARRRAEAKLRANEAR
FRTMVNTIPQLAWIGETDGSRSWYNQRWYDYTGTTPEEMMGWGWQKVHDPQLLPEVIKRWSEATAAEQPLDMEIPLRGAD
GELRVFLTRAEPLRNEDGQVVQWFGTNTDVHDQRLREEAQRETEKRLQVVMEHMSEGLIISDFNRNLLRWNPAALRMHEI
ENAEEVFGSLHNFARIYELSTTEGNVVPIDQWPLTRILRGEHLHDLELRIRRLDKEWLRIFSYSGTVLRYENSKGLAFLT
IKDITERKQAEEALRDAKVNLEYKVNERTAQLLAKSKELENFCYSVSHDLRAPLRGIAGYSRLLLENYYERLDEEGRSFL
TNVRSATKHMTDLIEDLLSYSKLERRKLSSMVIRLPAFVSKVLAQFGDNLQNVRLTVSMDDFHVRADPDGLAIALRNLVD
NAVKFSIHAPEPTIEIRAWTSDSNCILCVRDNGIGFDMRFYDKIFEIFQRLQRAEEYPGTGIGLAMVQKAMERMGGRVWA
ESQVGAGAVFYLQLPLSGLMPFEQLQEQAL

Specific function: Photoreceptor which exists in two forms that are reversibly interconvertible by light:the R form that absorbs maximally in the red region of the spectrum and the FR form that absorbs maximally in the far-red region [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=264, Percent_Identity=29.1666666666667, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789149, Length=248, Percent_Identity=29.0322580645161, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI145693157, Length=388, Percent_Identity=24.4845360824742, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788549, Length=421, Percent_Identity=25.8907363420428, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=30.9417040358744, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788713, Length=265, Percent_Identity=24.1509433962264, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1790861, Length=240, Percent_Identity=28.3333333333333, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI87081816, Length=225, Percent_Identity=28.4444444444444, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1786912, Length=253, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1790346, Length=224, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1790436, Length=247, Percent_Identity=28.7449392712551, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003018
- InterPro:   IPR013654
- InterPro:   IPR016132
- InterPro:   IPR001294
- InterPro:   IPR013515
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF01590 GAF; PF02518 HATPase_c; PF00512 HisKA; PF08446 PAS_2; PF00360 Phytochrome [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 77308; Mature: 77308

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLNQNYTGSVACASENARDMTRLTTVENVRTPIAEILDQSLNEIYLLNAQTLHFEYAS
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEEEEHHHH
RGARRNLGYSMDSLQKMTLLEVEREFDETSFRALIDPLMKGGKEILVFEATHLRADGTHY
CCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCC
PVEVHLELSTRPEQVQFLVMAFDLTARRRAEAKLRANEARFRTMVNTIPQLAWIGETDGS
CEEEEEEECCCCCHHEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCC
RSWYNQRWYDYTGTTPEEMMGWGWQKVHDPQLLPEVIKRWSEATAAEQPLDMEIPLRGAD
CCHHCCCEECCCCCCHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
GELRVFLTRAEPLRNEDGQVVQWFGTNTDVHDQRLREEAQRETEKRLQVVMEHMSEGLII
CCEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
SDFNRNLLRWNPAALRMHEIENAEEVFGSLHNFARIYELSTTEGNVVPIDQWPLTRILRG
EECCCCCEEECHHHHHHHHHCCHHHHHHHHHHHHHEEEEECCCCCEEECCCCCHHHHHCC
EHLHDLELRIRRLDKEWLRIFSYSGTVLRYENSKGLAFLTIKDITERKQAEEALRDAKVN
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCEEE
LEYKVNERTAQLLAKSKELENFCYSVSHDLRAPLRGIAGYSRLLLENYYERLDEEGRSFL
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNVRSATKHMTDLIEDLLSYSKLERRKLSSMVIRLPAFVSKVLAQFGDNLQNVRLTVSMD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEC
DFHVRADPDGLAIALRNLVDNAVKFSIHAPEPTIEIRAWTSDSNCILCVRDNGIGFDMRF
CEEEEECCCHHHHHHHHHHHHHEEEEEECCCCEEEEEEEECCCCEEEEEECCCCCCCHHH
YDKIFEIFQRLQRAEEYPGTGIGLAMVQKAMERMGGRVWAESQVGAGAVFYLQLPLSGLM
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEECCHHHCC
PFEQLQEQAL
CHHHHHHHCC
>Mature Secondary Structure
MISLNQNYTGSVACASENARDMTRLTTVENVRTPIAEILDQSLNEIYLLNAQTLHFEYAS
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEEEEHHHH
RGARRNLGYSMDSLQKMTLLEVEREFDETSFRALIDPLMKGGKEILVFEATHLRADGTHY
CCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCC
PVEVHLELSTRPEQVQFLVMAFDLTARRRAEAKLRANEARFRTMVNTIPQLAWIGETDGS
CEEEEEEECCCCCHHEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCC
RSWYNQRWYDYTGTTPEEMMGWGWQKVHDPQLLPEVIKRWSEATAAEQPLDMEIPLRGAD
CCHHCCCEECCCCCCHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
GELRVFLTRAEPLRNEDGQVVQWFGTNTDVHDQRLREEAQRETEKRLQVVMEHMSEGLII
CCEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
SDFNRNLLRWNPAALRMHEIENAEEVFGSLHNFARIYELSTTEGNVVPIDQWPLTRILRG
EECCCCCEEECHHHHHHHHHCCHHHHHHHHHHHHHEEEEECCCCCEEECCCCCHHHHHCC
EHLHDLELRIRRLDKEWLRIFSYSGTVLRYENSKGLAFLTIKDITERKQAEEALRDAKVN
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCEEE
LEYKVNERTAQLLAKSKELENFCYSVSHDLRAPLRGIAGYSRLLLENYYERLDEEGRSFL
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNVRSATKHMTDLIEDLLSYSKLERRKLSSMVIRLPAFVSKVLAQFGDNLQNVRLTVSMD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEC
DFHVRADPDGLAIALRNLVDNAVKFSIHAPEPTIEIRAWTSDSNCILCVRDNGIGFDMRF
CEEEEECCCHHHHHHHHHHHHHEEEEEECCCCEEEEEEEECCCCEEEEEECCCCCCCHHH
YDKIFEIFQRLQRAEEYPGTGIGLAMVQKAMERMGGRVWAESQVGAGAVFYLQLPLSGLM
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEECCHHHCC
PFEQLQEQAL
CHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]