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The map label for this gene is aprF [H]

Identifier: 82703401

GI number: 82703401

Start: 2597227

End: 2598669

Strand: Reverse

Name: aprF [H]

Synonym: Nmul_A2283

Alternate gene names: 82703401

Gene position: 2598669-2597227 (Counterclockwise)

Preceding gene: 82703402

Following gene: 82703400

Centisome position: 81.61

GC content: 57.24

Gene sequence:

>1443_bases
ATGTTCCTTTCACGCAGCGCAAGGCAATCCGGAACTAAATCCAAGATTGCGCCGTACGTCCATGCGCTCCTGTTTATCGG
AGCGCTGAGCACTCCGCTGCGAGCTGCGGACATGATGGACATCTATCATGAAGCGTTGGAAAATGACGCGCAGTTTCGCG
CAGCCCGCTTCACGCACCAGGCTGCCCAGGAAAAGCTGCCCCAGGGCCGGGCGGGTCTGCTGCCCACGGTAACGCTGGCG
GGTGTGCGCCGCCGGCAGTGGATCGATATAGAAAGAATCGGCGGAACTGGAACCGCGACTGGAACGGGGGTGGCCGTCAG
GCCTTCCGAAGTTGTCATCGATAACCAGAGCCTGACCATTACCGCCACTCAGCCGATCTATCGCAAGGAGAATTTCGCGA
TCTACGAACAATCGAAGCTTCAGGTTGCACAGGCCGATTCGGAGTTCATCATGGCGGCGCAGGATCTGATCCTGCGGGTA
GCGCAGGCATATCTGGATATTCTGTTGGCGGAAGTAAACGTGGAGGTTGCAGAGGCGCAGAAAAAAGCCATCAGCGAGCA
ACTTGCACAGGCAAAGCGCAACTTCGAAGTCGGGACTGCTACCATCGTCGATACTCATGAAGCTCAGGCGCGTTTCGATC
TGACGGCTGCGCTGGAAATAGGCGCACTGAATGAACTTGAAGTGCGTAAAAGAACGCTCGAACAGATCATCGGGCGGATA
CCGGAAGATCTGGCCCATCCCACGGAGACACCCTCCGACCTGCTTACGCTGAAGTACCCCAGGATGCAGGATTGGATCAG
CGTGGCCGAACAGAACAATCTCGCGCTGAAGGTCCAGGAGGCGGTGTACGAGATTGCAAAGAAGGACGTTGAACGGGCTC
AGGCGGGGCATTATCCGACGCTCGATCTCGTTGCGATATACAGCGACCAGAAAGGGGTGGGGGGTACGATCACAGGGCGT
CCCATTGATCTGACCTCAAAAGAAATCGGGGTACAGCTCAGCTTTCCGCTGTTTCAGGGTTTTGCCGTGCAGTCGCGCGT
GCGCGAAGCAGTGGCTATCCAGGAAAAGGTGTTGCAGGACCTGAACAATACCCGCCGCAACAGCGTACTGCAGGTAAGCC
AGCAGTATCTCAATGTCACCAACGGCATTGCACAGGTAACGGCGCTGAAGCAGGCGCTGGTATCCAGTCAGAGCCAGGTC
GATTCCACCAAACTTGGCCAGGAAGTAGGCGTCAGAACCGAGGTGGACGTGCTGAATGCCCAGCAATTGTATTATTCCGC
GCGGCGGGATCTCGCGCAGGCGCGCTACAATTTTCTCATGTCACGTTTGCGGTTGAAGGCAGAGGCGGGAGAACTGGACG
AGGAGGATTTGAAGGAGGTGAACGATGCGCTGTTTCGACCCCAATCCCCGGGTCCCCTCCAGTCACCGCTCGCCGCGCAT
TGA

Upstream 100 bases:

>100_bases
TCTGATCTTTCGGCCCGCACTTCAGGATTGCCGTCCACGCGACGTATCCAGGGGTACTCTCCTATAGCTATCCGTCTATC
TTTAACTGTAGTCAACGAAG

Downstream 100 bases:

>100_bases
CGGGCGGCTGCCCCGGTGGTAGATTTTCAGTGGAAGATATCGCCTTTTCTCACTGAAACGTCAAAGGTGGCCATGCACAG
CGATGGGCACGCAGTCGAAG

Product: Type I secretion outer membrane protein TolC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MFLSRSARQSGTKSKIAPYVHALLFIGALSTPLRAADMMDIYHEALENDAQFRAARFTHQAAQEKLPQGRAGLLPTVTLA
GVRRRQWIDIERIGGTGTATGTGVAVRPSEVVIDNQSLTITATQPIYRKENFAIYEQSKLQVAQADSEFIMAAQDLILRV
AQAYLDILLAEVNVEVAEAQKKAISEQLAQAKRNFEVGTATIVDTHEAQARFDLTAALEIGALNELEVRKRTLEQIIGRI
PEDLAHPTETPSDLLTLKYPRMQDWISVAEQNNLALKVQEAVYEIAKKDVERAQAGHYPTLDLVAIYSDQKGVGGTITGR
PIDLTSKEIGVQLSFPLFQGFAVQSRVREAVAIQEKVLQDLNNTRRNSVLQVSQQYLNVTNGIAQVTALKQALVSSQSQV
DSTKLGQEVGVRTEVDVLNAQQLYYSARRDLAQARYNFLMSRLRLKAEAGELDEEDLKEVNDALFRPQSPGPLQSPLAAH

Sequences:

>Translated_480_residues
MFLSRSARQSGTKSKIAPYVHALLFIGALSTPLRAADMMDIYHEALENDAQFRAARFTHQAAQEKLPQGRAGLLPTVTLA
GVRRRQWIDIERIGGTGTATGTGVAVRPSEVVIDNQSLTITATQPIYRKENFAIYEQSKLQVAQADSEFIMAAQDLILRV
AQAYLDILLAEVNVEVAEAQKKAISEQLAQAKRNFEVGTATIVDTHEAQARFDLTAALEIGALNELEVRKRTLEQIIGRI
PEDLAHPTETPSDLLTLKYPRMQDWISVAEQNNLALKVQEAVYEIAKKDVERAQAGHYPTLDLVAIYSDQKGVGGTITGR
PIDLTSKEIGVQLSFPLFQGFAVQSRVREAVAIQEKVLQDLNNTRRNSVLQVSQQYLNVTNGIAQVTALKQALVSSQSQV
DSTKLGQEVGVRTEVDVLNAQQLYYSARRDLAQARYNFLMSRLRLKAEAGELDEEDLKEVNDALFRPQSPGPLQSPLAAH
>Mature_480_residues
MFLSRSARQSGTKSKIAPYVHALLFIGALSTPLRAADMMDIYHEALENDAQFRAARFTHQAAQEKLPQGRAGLLPTVTLA
GVRRRQWIDIERIGGTGTATGTGVAVRPSEVVIDNQSLTITATQPIYRKENFAIYEQSKLQVAQADSEFIMAAQDLILRV
AQAYLDILLAEVNVEVAEAQKKAISEQLAQAKRNFEVGTATIVDTHEAQARFDLTAALEIGALNELEVRKRTLEQIIGRI
PEDLAHPTETPSDLLTLKYPRMQDWISVAEQNNLALKVQEAVYEIAKKDVERAQAGHYPTLDLVAIYSDQKGVGGTITGR
PIDLTSKEIGVQLSFPLFQGFAVQSRVREAVAIQEKVLQDLNNTRRNSVLQVSQQYLNVTNGIAQVTALKQALVSSQSQV
DSTKLGQEVGVRTEVDVLNAQQLYYSARRDLAQARYNFLMSRLRLKAEAGELDEEDLKEVNDALFRPQSPGPLQSPLAAH

Specific function: Involved in the secretion of alkaline protease [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=450, Percent_Identity=32.2222222222222, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53040; Mature: 53040

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLSRSARQSGTKSKIAPYVHALLFIGALSTPLRAADMMDIYHEALENDAQFRAARFTHQ
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AAQEKLPQGRAGLLPTVTLAGVRRRQWIDIERIGGTGTATGTGVAVRPSEVVIDNQSLTI
HHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCEEECCCEEEEECCEEEE
TATQPIYRKENFAIYEQSKLQVAQADSEFIMAAQDLILRVAQAYLDILLAEVNVEVAEAQ
EECCCCCCCCCEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
KKAISEQLAQAKRNFEVGTATIVDTHEAQARFDLTAALEIGALNELEVRKRTLEQIIGRI
HHHHHHHHHHHHHCCCCCEEEEEECCHHHHHEEEHEEEECCCCCHHHHHHHHHHHHHHHC
PEDLAHPTETPSDLLTLKYPRMQDWISVAEQNNLALKVQEAVYEIAKKDVERAQAGHYPT
CHHHCCCCCCCCHHEEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCC
LDLVAIYSDQKGVGGTITGRPIDLTSKEIGVQLSFPLFQGFAVQSRVREAVAIQEKVLQD
EEEEEEECCCCCCCCCCCCCCCCCCHHHHCEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
LNNTRRNSVLQVSQQYLNVTNGIAQVTALKQALVSSQSQVDSTKLGQEVGVRTEVDVLNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
QQLYYSARRDLAQARYNFLMSRLRLKAEAGELDEEDLKEVNDALFRPQSPGPLQSPLAAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFLSRSARQSGTKSKIAPYVHALLFIGALSTPLRAADMMDIYHEALENDAQFRAARFTHQ
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AAQEKLPQGRAGLLPTVTLAGVRRRQWIDIERIGGTGTATGTGVAVRPSEVVIDNQSLTI
HHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCEEECCCEEEEECCEEEE
TATQPIYRKENFAIYEQSKLQVAQADSEFIMAAQDLILRVAQAYLDILLAEVNVEVAEAQ
EECCCCCCCCCEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
KKAISEQLAQAKRNFEVGTATIVDTHEAQARFDLTAALEIGALNELEVRKRTLEQIIGRI
HHHHHHHHHHHHHCCCCCEEEEEECCHHHHHEEEHEEEECCCCCHHHHHHHHHHHHHHHC
PEDLAHPTETPSDLLTLKYPRMQDWISVAEQNNLALKVQEAVYEIAKKDVERAQAGHYPT
CHHHCCCCCCCCHHEEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCC
LDLVAIYSDQKGVGGTITGRPIDLTSKEIGVQLSFPLFQGFAVQSRVREAVAIQEKVLQD
EEEEEEECCCCCCCCCCCCCCCCCCHHHHCEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
LNNTRRNSVLQVSQQYLNVTNGIAQVTALKQALVSSQSQVDSTKLGQEVGVRTEVDVLNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
QQLYYSARRDLAQARYNFLMSRLRLKAEAGELDEEDLKEVNDALFRPQSPGPLQSPLAAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1427098; 10984043 [H]