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The map label for this gene is purB [H]

Identifier: 82703359

GI number: 82703359

Start: 2546609

End: 2547991

Strand: Reverse

Name: purB [H]

Synonym: Nmul_A2241

Alternate gene names: 82703359

Gene position: 2547991-2546609 (Counterclockwise)

Preceding gene: 82703361

Following gene: 82703358

Centisome position: 80.02

GC content: 53.58

Gene sequence:

>1383_bases
ATGAATTCATCCTTTCTCACCGCACTCTCTCCTCTTGATGGCCGCTATCACGGCAAAGTCGATGCACTGAGACCCTATTT
CAGCGAACTCGGGCTGATCCGTTACCGCGTCCAGCTTGAGATCGAATGGCTCAAGGCATTGAGCCGGGCTTCCGCCATTG
CTGAAGCACTGCCGTTATCTCCGGATACGCTTGCACAGCTTGATGCTCTGATAACGGATTTTTCCGAAAGGGATGGCGAA
GCGGTCAAACTCATCGAGGCGCGCACCAATCATGACGTGAAAGCCGTGGAATACTGGCTTCGCAAGCAGTTAACGGAAAA
TGCCGAGATCGGCAGAATCGAACAATTCATTCATTTTGCCTGCACTTCAGAGGACATCAACAATCTTTCCCATGGATTGA
TGCTGATGCACAGCCGCGACGACATCATGTTGCCGGCGCTGGATAACATCATTGGCAGGCTCGTCCGGCTTGCGCACGAG
CTGGCCGCAGTGCCCATGCTTGCACGCACGCACGGGCAGGCTGCCACGCCGACCACGGTGGGCAAGGAGCTCGCCAATTT
CGCTTATCGTCTGCAACGGGGACGCCGGCGCTTGGCGCAGGTGGCTATTCTCGGAAAAATCAATGGAGCTGTGGGTAATT
ACAACGCCCACCTGGCGGCCTATCCCGACTTCGGATGGGAGAAATTCGCGCAGGATTTTGTGGAAAAGCTCGGCCTGCAA
TTCAATCCTTATACTACCCAGATTGAACCGCACGACACCGTAGCTGAACTGTTCGATGCCTATGCCAGGATCAATACGAT
TCTGCTGGATTTCAACCGGGATATATGGGGATACATCTCGCTAGCCTACTTCAAGCAAAAAACCCGAAAAGATGAAGTCG
GTTCCTCAACCATGCCCCACAAGGTCAATCCGATAGATTTTGAAAATTCCGAAGGTAATCTGGGGATTGCCAACACGCTC
CTGCGGCACTTGAGCGAGAAGCTGCCGATATCGCGCTGGCAGCGAGACCTTACCGACTCCACTGCACTGCGTAACATGGG
TGTCGCATTAGGCCATACTTTATTGGCATATGACTCCTGCAGCAGGGGCCTGGACAAACTGGAAATCAACCCCGGCCGTC
TGGAGGAGGATTTGAGAAATGCCTGGGAAGTGCTGGCCGAGCCCATTCAGACGGTGATGCGTCGCCACGGCATGCCGGAT
TCATACGAGCGGTTGAAAGAACTGACCCGGGGCAAGGGCGGTATCACCCAAGACGCGCTCCATCAGTTTATTGACAGTCT
CGCCCTTCCTGATGTGGAAAAAAAACGATTGCGTGAAATGAGGCCGGAAACATACCTGGGGAATGCTGCTGCATTAGCGA
GAAAAATCAGTACGGAATACTGA

Upstream 100 bases:

>100_bases
TTGAAGAGAGTGGTCAGCGCGCATTATATCGGCTTTCCGCACTGCATGGCATAACGGGGCCGAAGCCTACCTGTGTTAAA
ATTTCGCCGGAAACCAAGCT

Downstream 100 bases:

>100_bases
TTCTCGCGTCCTTTCCTTTTCTATCAAGCTGTCCCCTCACATTATGGAGCCCTGAAAATTTTGGGCTTGAAATTCGAGAA
ACCATCCCGATATGTTTAGG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNSSFLTALSPLDGRYHGKVDALRPYFSELGLIRYRVQLEIEWLKALSRASAIAEALPLSPDTLAQLDALITDFSERDGE
AVKLIEARTNHDVKAVEYWLRKQLTENAEIGRIEQFIHFACTSEDINNLSHGLMLMHSRDDIMLPALDNIIGRLVRLAHE
LAAVPMLARTHGQAATPTTVGKELANFAYRLQRGRRRLAQVAILGKINGAVGNYNAHLAAYPDFGWEKFAQDFVEKLGLQ
FNPYTTQIEPHDTVAELFDAYARINTILLDFNRDIWGYISLAYFKQKTRKDEVGSSTMPHKVNPIDFENSEGNLGIANTL
LRHLSEKLPISRWQRDLTDSTALRNMGVALGHTLLAYDSCSRGLDKLEINPGRLEEDLRNAWEVLAEPIQTVMRRHGMPD
SYERLKELTRGKGGITQDALHQFIDSLALPDVEKKRLREMRPETYLGNAAALARKISTEY

Sequences:

>Translated_460_residues
MNSSFLTALSPLDGRYHGKVDALRPYFSELGLIRYRVQLEIEWLKALSRASAIAEALPLSPDTLAQLDALITDFSERDGE
AVKLIEARTNHDVKAVEYWLRKQLTENAEIGRIEQFIHFACTSEDINNLSHGLMLMHSRDDIMLPALDNIIGRLVRLAHE
LAAVPMLARTHGQAATPTTVGKELANFAYRLQRGRRRLAQVAILGKINGAVGNYNAHLAAYPDFGWEKFAQDFVEKLGLQ
FNPYTTQIEPHDTVAELFDAYARINTILLDFNRDIWGYISLAYFKQKTRKDEVGSSTMPHKVNPIDFENSEGNLGIANTL
LRHLSEKLPISRWQRDLTDSTALRNMGVALGHTLLAYDSCSRGLDKLEINPGRLEEDLRNAWEVLAEPIQTVMRRHGMPD
SYERLKELTRGKGGITQDALHQFIDSLALPDVEKKRLREMRPETYLGNAAALARKISTEY
>Mature_460_residues
MNSSFLTALSPLDGRYHGKVDALRPYFSELGLIRYRVQLEIEWLKALSRASAIAEALPLSPDTLAQLDALITDFSERDGE
AVKLIEARTNHDVKAVEYWLRKQLTENAEIGRIEQFIHFACTSEDINNLSHGLMLMHSRDDIMLPALDNIIGRLVRLAHE
LAAVPMLARTHGQAATPTTVGKELANFAYRLQRGRRRLAQVAILGKINGAVGNYNAHLAAYPDFGWEKFAQDFVEKLGLQ
FNPYTTQIEPHDTVAELFDAYARINTILLDFNRDIWGYISLAYFKQKTRKDEVGSSTMPHKVNPIDFENSEGNLGIANTL
LRHLSEKLPISRWQRDLTDSTALRNMGVALGHTLLAYDSCSRGLDKLEINPGRLEEDLRNAWEVLAEPIQTVMRRHGMPD
SYERLKELTRGKGGITQDALHQFIDSLALPDVEKKRLREMRPETYLGNAAALARKISTEY

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=374, Percent_Identity=24.5989304812834, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI183227688, Length=362, Percent_Identity=25.1381215469613, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1787376, Length=456, Percent_Identity=58.5526315789474, Blast_Score=572, Evalue=1e-164,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51992; Mature: 51992

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSFLTALSPLDGRYHGKVDALRPYFSELGLIRYRVQLEIEWLKALSRASAIAEALPLS
CCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEHEEEHHHHHHHHHHHHHHHHHCCCC
PDTLAQLDALITDFSERDGEAVKLIEARTNHDVKAVEYWLRKQLTENAEIGRIEQFIHFA
CHHHHHHHHHHHHHHHCCCCEEEEEHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
CTSEDINNLSHGLMLMHSRDDIMLPALDNIIGRLVRLAHELAAVPMLARTHGQAATPTTV
HCHHHHHHHHHCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GKELANFAYRLQRGRRRLAQVAILGKINGAVGNYNAHLAAYPDFGWEKFAQDFVEKLGLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCC
FNPYTTQIEPHDTVAELFDAYARINTILLDFNRDIWGYISLAYFKQKTRKDEVGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGIANTLLRHLSEKLPISRWQRDLTDSTALRNMGVALGHTLLAYDSC
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRGLDKLEINPGRLEEDLRNAWEVLAEPIQTVMRRHGMPDSYERLKELTRGKGGITQDAL
HCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHH
HQFIDSLALPDVEKKRLREMRPETYLGNAAALARKISTEY
HHHHHHHCCCCHHHHHHHHCCCCHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNSSFLTALSPLDGRYHGKVDALRPYFSELGLIRYRVQLEIEWLKALSRASAIAEALPLS
CCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEHEEEHHHHHHHHHHHHHHHHHCCCC
PDTLAQLDALITDFSERDGEAVKLIEARTNHDVKAVEYWLRKQLTENAEIGRIEQFIHFA
CHHHHHHHHHHHHHHHCCCCEEEEEHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
CTSEDINNLSHGLMLMHSRDDIMLPALDNIIGRLVRLAHELAAVPMLARTHGQAATPTTV
HCHHHHHHHHHCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GKELANFAYRLQRGRRRLAQVAILGKINGAVGNYNAHLAAYPDFGWEKFAQDFVEKLGLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCC
FNPYTTQIEPHDTVAELFDAYARINTILLDFNRDIWGYISLAYFKQKTRKDEVGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGIANTLLRHLSEKLPISRWQRDLTDSTALRNMGVALGHTLLAYDSC
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRGLDKLEINPGRLEEDLRNAWEVLAEPIQTVMRRHGMPDSYERLKELTRGKGGITQDAL
HCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHH
HQFIDSLALPDVEKKRLREMRPETYLGNAAALARKISTEY
HHHHHHHCCCCHHHHHHHHCCCCHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]