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The map label for this gene is grpE

Identifier: 82703358

GI number: 82703358

Start: 2545905

End: 2546486

Strand: Reverse

Name: grpE

Synonym: Nmul_A2240

Alternate gene names: 82703358

Gene position: 2546486-2545905 (Counterclockwise)

Preceding gene: 82703359

Following gene: 82703357

Centisome position: 79.97

GC content: 53.09

Gene sequence:

>582_bases
ATGACAGAAGAAAACCGACCTCAACCAGACCAGCCCGAGCTGACCGTAACAAGCGAAAGCAGTGTACAGGAAACCGGGGA
AAACAAGGCCAGGACGCCCGAGCAGGAGGGCGAAGCCATGCCCAGCCTGGAGCAGTTATTGAAAAAAGCCGAACTCGATG
CAGCGGAACATTATGATGCATGGCTTCGCGCCAAGGCTGAAGGCGAGAACATAAGAAAGCGCGCCCAGATGGACGTCACC
AACGCGCACAAATACGCTATTGAAAATTTTTCCACTGAACTGCTCTCGGTCATGGACAGCCTGGAAGCTGCGTTGGCGGT
GGAGAATGCAACAGTCGAGAATTTCAAAAGCGGCATGGAATTGACGCTCAAGCAGCTCACTGCGACTTTCGCCAAGTTCA
ATATCAAGCAGCTCAGTCCCCAGGGTGAAAAATTCGATCCGCATTTGCACCAGGCCATGTGCATGGTGGAGTCCGAGCTT
CCGCACAACACAGTCGTGCAGGTTATGCAGAAAGGCTATGTGCTGAATGATCGGGTTATCCGGCCGGCGCTGGTTTCGGT
TTCGAAGGGAAAAGAGTCTTGA

Upstream 100 bases:

>100_bases
ATCAAGCTGTCCCCTCACATTATGGAGCCCTGAAAATTTTGGGCTTGAAATTCGAGAAACCATCCCGATATGTTTAGGGA
TAGAAAATCAGGAGAAAGCC

Downstream 100 bases:

>100_bases
AATCGCGATCTTTATCCCCACTTCAGCGGTAACAAGCGAATTATTCAACGGATATCGAGAATTAACTAATACAAGGATCA
ATTATGGGAAAAATTATCGG

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MTEENRPQPDQPELTVTSESSVQETGENKARTPEQEGEAMPSLEQLLKKAELDAAEHYDAWLRAKAEGENIRKRAQMDVT
NAHKYAIENFSTELLSVMDSLEAALAVENATVENFKSGMELTLKQLTATFAKFNIKQLSPQGEKFDPHLHQAMCMVESEL
PHNTVVQVMQKGYVLNDRVIRPALVSVSKGKES

Sequences:

>Translated_193_residues
MTEENRPQPDQPELTVTSESSVQETGENKARTPEQEGEAMPSLEQLLKKAELDAAEHYDAWLRAKAEGENIRKRAQMDVT
NAHKYAIENFSTELLSVMDSLEAALAVENATVENFKSGMELTLKQLTATFAKFNIKQLSPQGEKFDPHLHQAMCMVESEL
PHNTVVQVMQKGYVLNDRVIRPALVSVSKGKES
>Mature_192_residues
TEENRPQPDQPELTVTSESSVQETGENKARTPEQEGEAMPSLEQLLKKAELDAAEHYDAWLRAKAEGENIRKRAQMDVTN
AHKYAIENFSTELLSVMDSLEAALAVENATVENFKSGMELTLKQLTATFAKFNIKQLSPQGEKFDPHLHQAMCMVESELP
HNTVVQVMQKGYVLNDRVIRPALVSVSKGKES

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=144, Percent_Identity=37.5, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI40255109, Length=137, Percent_Identity=35.7664233576642, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1788967, Length=138, Percent_Identity=45.6521739130435, Blast_Score=119, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17552458, Length=191, Percent_Identity=31.9371727748691, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324806, Length=189, Percent_Identity=31.7460317460317, Blast_Score=90, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653432, Length=169, Percent_Identity=36.094674556213, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_NITMU (Q2Y6T9)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412924.1
- ProteinModelPortal:   Q2Y6T9
- SMR:   Q2Y6T9
- STRING:   Q2Y6T9
- GeneID:   3784941
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2240
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- PhylomeDB:   Q2Y6T9
- BioCyc:   NMUL323848:NMUL_A2240-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21586; Mature: 21454

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEENRPQPDQPELTVTSESSVQETGENKARTPEQEGEAMPSLEQLLKKAELDAAEHYDA
CCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHCCHHHHHHH
WLRAKAEGENIRKRAQMDVTNAHKYAIENFSTELLSVMDSLEAALAVENATVENFKSGME
HHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTLKQLTATFAKFNIKQLSPQGEKFDPHLHQAMCMVESELPHNTVVQVMQKGYVLNDRVI
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHH
RPALVSVSKGKES
HHHHHHHCCCCCC
>Mature Secondary Structure 
TEENRPQPDQPELTVTSESSVQETGENKARTPEQEGEAMPSLEQLLKKAELDAAEHYDA
CCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHCCHHHHHHH
WLRAKAEGENIRKRAQMDVTNAHKYAIENFSTELLSVMDSLEAALAVENATVENFKSGME
HHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTLKQLTATFAKFNIKQLSPQGEKFDPHLHQAMCMVESELPHNTVVQVMQKGYVLNDRVI
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHH
RPALVSVSKGKES
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA