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The map label for this gene is ligT [H]

Identifier: 82703244

GI number: 82703244

Start: 2419751

End: 2420338

Strand: Reverse

Name: ligT [H]

Synonym: Nmul_A2125

Alternate gene names: 82703244

Gene position: 2420338-2419751 (Counterclockwise)

Preceding gene: 82703245

Following gene: 82703242

Centisome position: 76.01

GC content: 52.38

Gene sequence:

>588_bases
ATGGCTGAAGAGACCCGGCAAAAAGCAGTACGCCTGTTTTTTGCGATATGGCCCGACAAGGAAACCTGCATGCAGCTGGA
TCGGCTGACCGGGCGATTGGCATCGGTTTGCGAGGGACGGAAAACAAAAGCGGAGAACATTCATCTTACATTGGTGTTTG
TAGGAGAGGTGAATGCAAGCCAGGTGGAGGCCCTGTGCCGGGCGGCGGATGAAATTGAAGGTCATGGCGTGAGAGCCTTC
GACCTCGTTATCGAAAGAATCTGCGTGTGGAAACGCAAGAATATCGTTTATGCGGAAATCAACGAGGTTCCCCGGCCACT
CATGGATCTGGTCGAGGCTTTGCAGAGCAGCCTTTCATTGGTAGGGTTTTCATTGGAGGAGCGGTCTTATAAGCCCCATA
TCACGCTCATGAGGAATGCATCCTGTAAAACGCTGCCCGAGCGGGCGGAACCAATGGTGTGGCGGGCGCGGGAATGGGTA
CTGGTTAAATCGGACCAGACCAGCGACGGTCCGGTTTATACACCGATTGGCCGCTGGTTCCTGAAAGGTGATCAAAGGAT
GATCAACTCTTTAAATCATCAAAGATAA

Upstream 100 bases:

>100_bases
GATTACCCACATGAATCAAACGACAGACTGAGGATGGAAACGGAGCGGAAAGCAAGCGGCGAAGGGACGGTAGATGACTG
GGGGATGAGCGGAAACCCGG

Downstream 100 bases:

>100_bases
ATGCAGCTCTCCAGTTGCTTTACCAGGTTTCTTTCATTGGCCCTGGTGTAATCCTTTTCAAAAGTATCGCTTACGAGGTT
GCGCACCTGGCTTCCCAGAT

Product: 2',5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MAEETRQKAVRLFFAIWPDKETCMQLDRLTGRLASVCEGRKTKAENIHLTLVFVGEVNASQVEALCRAADEIEGHGVRAF
DLVIERICVWKRKNIVYAEINEVPRPLMDLVEALQSSLSLVGFSLEERSYKPHITLMRNASCKTLPERAEPMVWRAREWV
LVKSDQTSDGPVYTPIGRWFLKGDQRMINSLNHQR

Sequences:

>Translated_195_residues
MAEETRQKAVRLFFAIWPDKETCMQLDRLTGRLASVCEGRKTKAENIHLTLVFVGEVNASQVEALCRAADEIEGHGVRAF
DLVIERICVWKRKNIVYAEINEVPRPLMDLVEALQSSLSLVGFSLEERSYKPHITLMRNASCKTLPERAEPMVWRAREWV
LVKSDQTSDGPVYTPIGRWFLKGDQRMINSLNHQR
>Mature_194_residues
AEETRQKAVRLFFAIWPDKETCMQLDRLTGRLASVCEGRKTKAENIHLTLVFVGEVNASQVEALCRAADEIEGHGVRAFD
LVIERICVWKRKNIVYAEINEVPRPLMDLVEALQSSLSLVGFSLEERSYKPHITLMRNASCKTLPERAEPMVWRAREWVL
VKSDQTSDGPVYTPIGRWFLKGDQRMINSLNHQR

Specific function: Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage [H]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081692, Length=172, Percent_Identity=33.7209302325581, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 22398; Mature: 22267

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEETRQKAVRLFFAIWPDKETCMQLDRLTGRLASVCEGRKTKAENIHLTLVFVGEVNAS
CCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCHH
QVEALCRAADEIEGHGVRAFDLVIERICVWKRKNIVYAEINEVPRPLMDLVEALQSSLSL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHCCHHHHHHHHHHHHHHHH
VGFSLEERSYKPHITLMRNASCKTLPERAEPMVWRAREWVLVKSDQTSDGPVYTPIGRWF
HCCCHHHCCCCCCEEEEECCCCCCCCCCCCCCEEECEEEEEEECCCCCCCCEECCCHHHH
LKGDQRMINSLNHQR
HCCHHHHHHHHCCCC
>Mature Secondary Structure 
AEETRQKAVRLFFAIWPDKETCMQLDRLTGRLASVCEGRKTKAENIHLTLVFVGEVNAS
CCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCHH
QVEALCRAADEIEGHGVRAFDLVIERICVWKRKNIVYAEINEVPRPLMDLVEALQSSLSL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHCCHHHHHHHHHHHHHHHH
VGFSLEERSYKPHITLMRNASCKTLPERAEPMVWRAREWVLVKSDQTSDGPVYTPIGRWF
HCCCHHHCCCCCCEEEEECCCCCCCCCCCCCCEEECEEEEEEECCCCCCCCEECCCHHHH
LKGDQRMINSLNHQR
HCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 2013578; 8940112 [H]