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The map label for this gene is ispF

Identifier: 82703245

GI number: 82703245

Start: 2420408

End: 2420941

Strand: Reverse

Name: ispF

Synonym: Nmul_A2126

Alternate gene names: 82703245

Gene position: 2420941-2420408 (Counterclockwise)

Preceding gene: 82703246

Following gene: 82703244

Centisome position: 76.03

GC content: 55.81

Gene sequence:

>534_bases
ATGTTTTTCGCGGAAATTAAACAAGAAGAGGTGATAGTGGGCGGATTCAGAATCGGGCAGGGTTTTGATGTGCACCAGTT
GGTCGAGGGGCGCAAGCTTGTTATCGGCGGAGTGACTATTCCCTACGAGAAAGGTTTGCTGGGGCATTCCGATGCGGATG
TGCTGCTGCATGCCATATGCGACGCGGTGCTGGGAGCCGCGGCACTGGGGGATATCGGCCGGCATTTTTCTGACACTGAT
CCGCGTTACAGGAATATCGACAGCCGCGTGCTACTGCAGAATGTAGGTAACCTCCTTGCGGAGCGTGGCTATAGGGTAGT
GAATGTTGATGCCACTATCATTGCGCAGGCACCCAGGATGGCCTCCCATATTCCCGCAATGGTCGCAAATATTGCGCAGG
ACCTGCGCATGCAGCCCGGAGATGTCAACGTCAAGGCCAAGACGGCCGAACGTCTTGGGCCGGTGGGGCGGGGTGAGGGA
ATAGAGGCGGAAGCGGTATGCCTGATTACCCACATGAATCAAACGACAGACTGA

Upstream 100 bases:

>100_bases
CCCGCTGAAAGCAGTTGTAGATCCAGGCGTCAGGTGAAAGATGTCGGGCAAAGCCCGAGAGTCGCGCAAAGCACCGATTA
CTGGAGGCTACTGTTTTCTC

Downstream 100 bases:

>100_bases
GGATGGAAACGGAGCGGAAAGCAAGCGGCGAAGGGACGGTAGATGACTGGGGGATGAGCGGAAACCCGGATGGCTGAAGA
GACCCGGCAAAAAGCAGTAC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MFFAEIKQEEVIVGGFRIGQGFDVHQLVEGRKLVIGGVTIPYEKGLLGHSDADVLLHAICDAVLGAAALGDIGRHFSDTD
PRYRNIDSRVLLQNVGNLLAERGYRVVNVDATIIAQAPRMASHIPAMVANIAQDLRMQPGDVNVKAKTAERLGPVGRGEG
IEAEAVCLITHMNQTTD

Sequences:

>Translated_177_residues
MFFAEIKQEEVIVGGFRIGQGFDVHQLVEGRKLVIGGVTIPYEKGLLGHSDADVLLHAICDAVLGAAALGDIGRHFSDTD
PRYRNIDSRVLLQNVGNLLAERGYRVVNVDATIIAQAPRMASHIPAMVANIAQDLRMQPGDVNVKAKTAERLGPVGRGEG
IEAEAVCLITHMNQTTD
>Mature_177_residues
MFFAEIKQEEVIVGGFRIGQGFDVHQLVEGRKLVIGGVTIPYEKGLLGHSDADVLLHAICDAVLGAAALGDIGRHFSDTD
PRYRNIDSRVLLQNVGNLLAERGYRVVNVDATIIAQAPRMASHIPAMVANIAQDLRMQPGDVNVKAKTAERLGPVGRGEG
IEAEAVCLITHMNQTTD

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=59.0909090909091, Blast_Score=169, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_NITMU (Q2Y752)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412811.1
- ProteinModelPortal:   Q2Y752
- SMR:   Q2Y752
- STRING:   Q2Y752
- GeneID:   3786664
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2126
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   Q2Y752
- ProtClustDB:   PRK00084
- BioCyc:   NMUL323848:NMUL_A2126-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 19079; Mature: 19079

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFAEIKQEEVIVGGFRIGQGFDVHQLVEGRKLVIGGVTIPYEKGLLGHSDADVLLHAIC
CCCCCCCCCEEEEEEEEECCCCCHHHHHCCCEEEEECEECCCCCCCCCCCHHHHHHHHHH
DAVLGAAALGDIGRHFSDTDPRYRNIDSRVLLQNVGNLLAERGYRVVNVDATIIAQAPRM
HHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCEEEECCHHEEECCCHH
ASHIPAMVANIAQDLRMQPGDVNVKAKTAERLGPVGRGEGIEAEAVCLITHMNQTTD
HHHHHHHHHHHHHHHCCCCCCCEEEECCHHHCCCCCCCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MFFAEIKQEEVIVGGFRIGQGFDVHQLVEGRKLVIGGVTIPYEKGLLGHSDADVLLHAIC
CCCCCCCCCEEEEEEEEECCCCCHHHHHCCCEEEEECEECCCCCCCCCCCHHHHHHHHHH
DAVLGAAALGDIGRHFSDTDPRYRNIDSRVLLQNVGNLLAERGYRVVNVDATIIAQAPRM
HHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCEEEECCHHEEECCCHH
ASHIPAMVANIAQDLRMQPGDVNVKAKTAERLGPVGRGEGIEAEAVCLITHMNQTTD
HHHHHHHHHHHHHHHCCCCCCCEEEECCHHHCCCCCCCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA