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The map label for this gene is dnaG [H]

Identifier: 82703178

GI number: 82703178

Start: 2351070

End: 2352848

Strand: Reverse

Name: dnaG [H]

Synonym: Nmul_A2058

Alternate gene names: 82703178

Gene position: 2352848-2351070 (Counterclockwise)

Preceding gene: 82703179

Following gene: 82703177

Centisome position: 73.89

GC content: 54.69

Gene sequence:

>1779_bases
ATGATTTCACAATCATTCATTCAGGAACTGCTCGACCGGACAGATATTGTCGATGTCATCGAGCGCGATGTGCCCCTCAG
GAGAGCAGGCGCGAACTATAGCGCCTGCTGCCCATTTCATAGTGAAAAAACTCCCTCCTTCACGGTAAGTCCCATCAAGC
AGTTTTATCATTGTTTCGGTTGCGGGGCCCACGGTAATGCCATTGGTTTCGTGATGGAGTACGATGGCATGCACTTCGTG
GAGGCTGTGAGTGAACTTGCGGCCCGAATCGGGCTCCAGGTGCCTGCGCAACAAGCTGAAACCGGATCAGGGTCTCAATC
AGATTCTGCACGGGTAAAGCCGAACGGAACAGAATACTCCCCGGGTGCGTTGCTGGAGGCAATGAAGATTGCCGCGCGTT
TTTACCGGGAGCAACTCAAGCTTTCGAAAAATGCGATCGACTACTTGAAGCAGCGGGGCTTGACGGGTGCAACCGCTGCG
CGCTTTGGCATCGGATATGCTCCCGCAGGTTGGCAGAACCTGCACACTTCATTCCCCGGGCAGCCGGATAATGCCCAAAA
ACGCCTGCTGGTACAGGCCGGATTGATGGTCGAGGGTGACGGAGGCAAGTGTTATGACCGGTTTCGTGATCGCATCATGT
TTCCCATCGTCAACCTCAAAGGGATGATTGTCGGATTTGGTGGCAGAGTGCTGGAGAAAGGAGAGCCCAAGTATCTCAAT
TCCCCGGAAACACCCCTATTCCAGAAAGGCCGCGAGCTCTATAATCTTTTTGGCGCCCGGCGGGCGATCCGGGAAGCGGG
AAAGGTTATCGTGGTGGAGGGCTACATGGATGTAGTGGCTCTTTCCCAGCATGGAATCGAATACGCGGTGGCTACCCTGG
GCACTGCCACCACCCCCTACCATATCCAGAAGCTGCTGCGCCAGACCGATAATATCGTCTTTTGCTTTGATGCAGACAGC
GCTGGAAAAAAAGCGGCTTGGCGCGCGCTGGAGGACAGCCTCGCCCAGCTTGCCGACGGCAAGAGCATCGGTTTTCTTTT
TCTGCCGGAGGGGGAGGATCCTGACAGTTACATCCGCAATCATGGCAGAAGCGCCTTCGAAAAGCTGCTTGAGCAAACAT
TGCCTCTGTCTGTTTTTCTTTTCAGGGAACTGTCGGCGCGAACCGATCTCAAAAGTAGCGAGGGGCGTGCCAAGCTGGTG
CAGGATGCCAAACCCTTGCTGGCGCGAGTCGCCGCACCCGGACTTGCACTGATGCTGGTGAAGCGATTGGCGGAGATCAG
TGGTCTGAGCGAGCGGGAGTTGGAAGGTATTTTGAAGATCAGACGCGTTACCGCTTTTCCGGTTCGCGAAAAGCCTTCCC
GTCCGAAGCCTGTTTCTCCCTACGAGTGGCTGCTCCAGATATTGTTGTACCACCCTGCTTATATCCAGAAGCTTGACAGG
GAACTGCTTGCATATGACCAGGAATATGAGAAAGAAGTAGCAGCGCTAGCGGCGCTCGTTGAATTTATCGACGTCCACCC
CCATATGGGAGAAGGGACGTTGATACCTTTGATGACTGTTCATTTCCGTGCTAGTCCCTACCGCGCAATCCTTGAACAGG
CGGCAAGCAAAACACTTGGCTGGGACGGCGAACTCGACCTGGAAGCGGAATTTGCCGGAGCGATGGCGCGATTACATGAG
ATGAAACGCAAGCAACGCATGGCAGTGCTGCACAATAAATCCCTGAGTGCCCTTACCCCCGAGGAAAGGGAGGAGCTTCA
GCGGATGGCAATGCCATAA

Upstream 100 bases:

>100_bases
CAAGTACTGCATGTATCAAAAAAGTGGAGTGCTCTTTTCTCGCACAAGTGAATTTTCTGCCCGCGCTCCACTCTCCCCAT
ACTTGCCTGATCTGTCATAA

Downstream 100 bases:

>100_bases
AGCAGAATTGTCAGCTGTGGTATGAATGCAAGCATGTTCTGATATAATAGAAAAATTTTTCCTTTCAACGGAAAGAACTC
AGAAAAGAATGTGGTCAAAC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MISQSFIQELLDRTDIVDVIERDVPLRRAGANYSACCPFHSEKTPSFTVSPIKQFYHCFGCGAHGNAIGFVMEYDGMHFV
EAVSELAARIGLQVPAQQAETGSGSQSDSARVKPNGTEYSPGALLEAMKIAARFYREQLKLSKNAIDYLKQRGLTGATAA
RFGIGYAPAGWQNLHTSFPGQPDNAQKRLLVQAGLMVEGDGGKCYDRFRDRIMFPIVNLKGMIVGFGGRVLEKGEPKYLN
SPETPLFQKGRELYNLFGARRAIREAGKVIVVEGYMDVVALSQHGIEYAVATLGTATTPYHIQKLLRQTDNIVFCFDADS
AGKKAAWRALEDSLAQLADGKSIGFLFLPEGEDPDSYIRNHGRSAFEKLLEQTLPLSVFLFRELSARTDLKSSEGRAKLV
QDAKPLLARVAAPGLALMLVKRLAEISGLSERELEGILKIRRVTAFPVREKPSRPKPVSPYEWLLQILLYHPAYIQKLDR
ELLAYDQEYEKEVAALAALVEFIDVHPHMGEGTLIPLMTVHFRASPYRAILEQAASKTLGWDGELDLEAEFAGAMARLHE
MKRKQRMAVLHNKSLSALTPEEREELQRMAMP

Sequences:

>Translated_592_residues
MISQSFIQELLDRTDIVDVIERDVPLRRAGANYSACCPFHSEKTPSFTVSPIKQFYHCFGCGAHGNAIGFVMEYDGMHFV
EAVSELAARIGLQVPAQQAETGSGSQSDSARVKPNGTEYSPGALLEAMKIAARFYREQLKLSKNAIDYLKQRGLTGATAA
RFGIGYAPAGWQNLHTSFPGQPDNAQKRLLVQAGLMVEGDGGKCYDRFRDRIMFPIVNLKGMIVGFGGRVLEKGEPKYLN
SPETPLFQKGRELYNLFGARRAIREAGKVIVVEGYMDVVALSQHGIEYAVATLGTATTPYHIQKLLRQTDNIVFCFDADS
AGKKAAWRALEDSLAQLADGKSIGFLFLPEGEDPDSYIRNHGRSAFEKLLEQTLPLSVFLFRELSARTDLKSSEGRAKLV
QDAKPLLARVAAPGLALMLVKRLAEISGLSERELEGILKIRRVTAFPVREKPSRPKPVSPYEWLLQILLYHPAYIQKLDR
ELLAYDQEYEKEVAALAALVEFIDVHPHMGEGTLIPLMTVHFRASPYRAILEQAASKTLGWDGELDLEAEFAGAMARLHE
MKRKQRMAVLHNKSLSALTPEEREELQRMAMP
>Mature_592_residues
MISQSFIQELLDRTDIVDVIERDVPLRRAGANYSACCPFHSEKTPSFTVSPIKQFYHCFGCGAHGNAIGFVMEYDGMHFV
EAVSELAARIGLQVPAQQAETGSGSQSDSARVKPNGTEYSPGALLEAMKIAARFYREQLKLSKNAIDYLKQRGLTGATAA
RFGIGYAPAGWQNLHTSFPGQPDNAQKRLLVQAGLMVEGDGGKCYDRFRDRIMFPIVNLKGMIVGFGGRVLEKGEPKYLN
SPETPLFQKGRELYNLFGARRAIREAGKVIVVEGYMDVVALSQHGIEYAVATLGTATTPYHIQKLLRQTDNIVFCFDADS
AGKKAAWRALEDSLAQLADGKSIGFLFLPEGEDPDSYIRNHGRSAFEKLLEQTLPLSVFLFRELSARTDLKSSEGRAKLV
QDAKPLLARVAAPGLALMLVKRLAEISGLSERELEGILKIRRVTAFPVREKPSRPKPVSPYEWLLQILLYHPAYIQKLDR
ELLAYDQEYEKEVAALAALVEFIDVHPHMGEGTLIPLMTVHFRASPYRAILEQAASKTLGWDGELDLEAEFAGAMARLHE
MKRKQRMAVLHNKSLSALTPEEREELQRMAMP

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=597, Percent_Identity=42.3785594639866, Blast_Score=443, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65832; Mature: 65832

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISQSFIQELLDRTDIVDVIERDVPLRRAGANYSACCPFHSEKTPSFTVSPIKQFYHCFG
CCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEECCCCCCCCCCEEHHHHHHHHHHHC
CGAHGNAIGFVMEYDGMHFVEAVSELAARIGLQVPAQQAETGSGSQSDSARVKPNGTEYS
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCC
PGALLEAMKIAARFYREQLKLSKNAIDYLKQRGLTGATAARFGIGYAPAGWQNLHTSFPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHCCCCCC
QPDNAQKRLLVQAGLMVEGDGGKCYDRFRDRIMFPIVNLKGMIVGFGGRVLEKGEPKYLN
CCCCHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCCCC
SPETPLFQKGRELYNLFGARRAIREAGKVIVVEGYMDVVALSQHGIEYAVATLGTATTPY
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCCHHHHHHHHCCCCCCH
HIQKLLRQTDNIVFCFDADSAGKKAAWRALEDSLAQLADGKSIGFLFLPEGEDPDSYIRN
HHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
HGRSAFEKLLEQTLPLSVFLFRELSARTDLKSSEGRAKLVQDAKPLLARVAAPGLALMLV
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
KRLAEISGLSERELEGILKIRRVTAFPVREKPSRPKPVSPYEWLLQILLYHPAYIQKLDR
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
ELLAYDQEYEKEVAALAALVEFIDVHPHMGEGTLIPLMTVHFRASPYRAILEQAASKTLG
HHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCC
WDGELDLEAEFAGAMARLHEMKRKQRMAVLHNKSLSALTPEEREELQRMAMP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MISQSFIQELLDRTDIVDVIERDVPLRRAGANYSACCPFHSEKTPSFTVSPIKQFYHCFG
CCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEECCCCCCCCCCEEHHHHHHHHHHHC
CGAHGNAIGFVMEYDGMHFVEAVSELAARIGLQVPAQQAETGSGSQSDSARVKPNGTEYS
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCC
PGALLEAMKIAARFYREQLKLSKNAIDYLKQRGLTGATAARFGIGYAPAGWQNLHTSFPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHCCCCCC
QPDNAQKRLLVQAGLMVEGDGGKCYDRFRDRIMFPIVNLKGMIVGFGGRVLEKGEPKYLN
CCCCHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCCCC
SPETPLFQKGRELYNLFGARRAIREAGKVIVVEGYMDVVALSQHGIEYAVATLGTATTPY
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCCHHHHHHHHCCCCCCH
HIQKLLRQTDNIVFCFDADSAGKKAAWRALEDSLAQLADGKSIGFLFLPEGEDPDSYIRN
HHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
HGRSAFEKLLEQTLPLSVFLFRELSARTDLKSSEGRAKLVQDAKPLLARVAAPGLALMLV
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
KRLAEISGLSERELEGILKIRRVTAFPVREKPSRPKPVSPYEWLLQILLYHPAYIQKLDR
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
ELLAYDQEYEKEVAALAALVEFIDVHPHMGEGTLIPLMTVHFRASPYRAILEQAASKTLG
HHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCC
WDGELDLEAEFAGAMARLHEMKRKQRMAVLHNKSLSALTPEEREELQRMAMP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]