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The map label for this gene is rpoD [H]

Identifier: 82703177

GI number: 82703177

Start: 2348764

End: 2350887

Strand: Reverse

Name: rpoD [H]

Synonym: Nmul_A2057

Alternate gene names: 82703177

Gene position: 2350887-2348764 (Counterclockwise)

Preceding gene: 82703178

Following gene: 82703176

Centisome position: 73.83

GC content: 53.81

Gene sequence:

>2124_bases
ATGGCAATCAAGGAATCTGAAGATATGAAGAAGCTGTTGAGAGCGCAGGCGAAAGAAGCGGCAGCAAAAGAACTGACGAA
GGTGGATGAAGAAATGTTCGATAATGACAGTGAAACGGAAACGGATTCAATGGCTAAAGCTCCGGAGCAGGTGACCGAGA
TTGCGAAGGCAGTGATGGCGGCGAAAGAGCCCACCCTCGCCAATGGCAAGATCGCAAGATCAAGAGCGGCCCGGGAAGGA
AAAAACTCGCACGCCAAGGATCCGGAAAAAGCTGCCGCCTCCCAGGAGCTCGAGGCGCGCCGCATGCGTCTCAAGAATCT
GATCGTGCAGGGCAAGGAACGCGGCTATCTCACTTACGCCGAAATCAATGATCATCTCCCCGACGATATGCTCGACGCGG
AGCAGATCGAGAACATCATCAGCATGATCAATGACGTCGGGATTTCCGTTTATGACGAGGCGCCCGACGCGGAAACGCTG
CTCATGTCTGAAACTGCCCCTACCGTGGCCGATGAGGATGTGGTGGAGGAAGCGGAAGCCGCGCTTTCCACTGTGGATTC
GGAGTTCGGGCGCACAACCGACCCTGTCCGGATGTATATGCGCGAAATGGGTTCCGTGGAACTGCTGACGCGTGAGAGCG
AAATCGAAATCGCAAAACGTATCGAGGACGGCTTGAAGCACATGATACAGGCGATTTCCGCCTGTCCGACAACCATAGCC
GGAATTCTCGAATTCGCCGACAGAGTGTCGAAAGACGAGATGCGTGTGGATGAGCTGGTGGATGGGTTGCTCGATCCCAG
CACAGAGGAAATTATCAGCGAAGAGATTTCCGATGAATCCCTGGAACAGGAATTGAACTCGGATGCGGAAGATGAGGATG
TCACCGCAGTCGCAAATGCCAACCTCCTCAAGCTGAAAAATGACGCGCTGGAGCGTTTTGCAGTGGTTCAAAAGGCTTAT
GACGAGATGCAGAAGGTGCTCGAAAAGAAAGGATCGGGCAACAAGGCCTATAAAGACATCCAGGAGCAGATTTCGTCCGA
GCTGATGGCTATCCGTTTCTCTGCCAAAATGGTTGAACGGTTGTGCGATACGCAGCGGGCACTGGTGGATGAAATGCGCG
GTTACGAGCGAAAAATAATGGAGCTTTGCGTAAGCAAGGTGGGAATGTCGCGTAACCACTTCATCAAGACCTTCCCCGGT
AACGAGAGTAACCTGAACTGGGTGGATGAGGAGATTGCACTCGGCAAACCCTACAGCGCAGCCCTGGAACGTTATCGTCC
CGAGATTGTGGAACAGCAACAGAATCTGCTGGCACTGCAAAAGCAGGTAGGCATTCCCTTGAAGGAACTCAAGGAAATCA
ACCGCCGCATGTCCACGGGTGAGGCGAAGGCGCGCCGGGCCAAACGTGAAATGACCGAAGCAAATTTGCGACTGGTGATT
TCCATCGCTAAAAAATATACCAATCGGGGATTGCAGTTCCTCGATCTCATTCAGGAAGGCAACATCGGCCTGATGAAGGC
AGTCGATAAATTCGAATACCGGCGGGGATACAAGTTTTCCACCTACGCAACCTGGTGGATTCGTCAGGCCATCACACGTT
CCATTGCGGATCAGGCGCGTACCATCCGTATCCCGGTGCACATGATCGAAACGATTAACAAGATGAACCGCATTTCCCGC
CAGATCCTGCAGGAAACCGGGCAGGAGCCGGAGCCCGCCGTCCTCGCACAGAAAATGGAAATGCCGGAAGAGAAGATTCG
TAAAATCCTCAAGATTTCCAAGGAACCAATTTCCATGGAGACCCCGATCGGAGACGACGAAGATTCTCATCTCGGGGATT
TCATCGAGGATTCAGCTACCATGGCTCCTGCGGATGCGGCAGTTTATGCCAGCCTGCGCGATGTTACGAAAGATATACTG
GATTCGCTGACTCCGCGCGAAGCAAAAGTACTGCGCATGCGCTTCGGCATCGAAATGAATACCGACCACACGCTGGAGGA
AGTCGGCAAGCAGTTCGACGTAACGCGCGAGCGCATCCGCCAGATCGAGGCCAAGGCACTGCGCAAGCTGCGCCATCCGT
CCCGTTCCGAGCGCCTGCGCAGCTTCCTGGATACTGAAGGCTGA

Upstream 100 bases:

>100_bases
AAAAGAATGTGGTCAAACCCGGACACAAGTCCAAACAGCGGTAAAAGCAAAGATGGCTGTGTTTGCCGGAGTTTCGGGTC
AACCTGAGGGCGAGGTTCGG

Downstream 100 bases:

>100_bases
CTAGCCTTCAGTATCCAGTTCAATACAGCTCCAAGTTAAGAGTTTTAAAGTTGTAAAGGGTCTGTAGCTCAGTCGGTTAG
AGCAGGGGACTCATAATCCC

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 707; Mature: 706

Protein sequence:

>707_residues
MAIKESEDMKKLLRAQAKEAAAKELTKVDEEMFDNDSETETDSMAKAPEQVTEIAKAVMAAKEPTLANGKIARSRAAREG
KNSHAKDPEKAAASQELEARRMRLKNLIVQGKERGYLTYAEINDHLPDDMLDAEQIENIISMINDVGISVYDEAPDAETL
LMSETAPTVADEDVVEEAEAALSTVDSEFGRTTDPVRMYMREMGSVELLTRESEIEIAKRIEDGLKHMIQAISACPTTIA
GILEFADRVSKDEMRVDELVDGLLDPSTEEIISEEISDESLEQELNSDAEDEDVTAVANANLLKLKNDALERFAVVQKAY
DEMQKVLEKKGSGNKAYKDIQEQISSELMAIRFSAKMVERLCDTQRALVDEMRGYERKIMELCVSKVGMSRNHFIKTFPG
NESNLNWVDEEIALGKPYSAALERYRPEIVEQQQNLLALQKQVGIPLKELKEINRRMSTGEAKARRAKREMTEANLRLVI
SIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISR
QILQETGQEPEPAVLAQKMEMPEEKIRKILKISKEPISMETPIGDDEDSHLGDFIEDSATMAPADAAVYASLRDVTKDIL
DSLTPREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSERLRSFLDTEG

Sequences:

>Translated_707_residues
MAIKESEDMKKLLRAQAKEAAAKELTKVDEEMFDNDSETETDSMAKAPEQVTEIAKAVMAAKEPTLANGKIARSRAAREG
KNSHAKDPEKAAASQELEARRMRLKNLIVQGKERGYLTYAEINDHLPDDMLDAEQIENIISMINDVGISVYDEAPDAETL
LMSETAPTVADEDVVEEAEAALSTVDSEFGRTTDPVRMYMREMGSVELLTRESEIEIAKRIEDGLKHMIQAISACPTTIA
GILEFADRVSKDEMRVDELVDGLLDPSTEEIISEEISDESLEQELNSDAEDEDVTAVANANLLKLKNDALERFAVVQKAY
DEMQKVLEKKGSGNKAYKDIQEQISSELMAIRFSAKMVERLCDTQRALVDEMRGYERKIMELCVSKVGMSRNHFIKTFPG
NESNLNWVDEEIALGKPYSAALERYRPEIVEQQQNLLALQKQVGIPLKELKEINRRMSTGEAKARRAKREMTEANLRLVI
SIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISR
QILQETGQEPEPAVLAQKMEMPEEKIRKILKISKEPISMETPIGDDEDSHLGDFIEDSATMAPADAAVYASLRDVTKDIL
DSLTPREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSERLRSFLDTEG
>Mature_706_residues
AIKESEDMKKLLRAQAKEAAAKELTKVDEEMFDNDSETETDSMAKAPEQVTEIAKAVMAAKEPTLANGKIARSRAAREGK
NSHAKDPEKAAASQELEARRMRLKNLIVQGKERGYLTYAEINDHLPDDMLDAEQIENIISMINDVGISVYDEAPDAETLL
MSETAPTVADEDVVEEAEAALSTVDSEFGRTTDPVRMYMREMGSVELLTRESEIEIAKRIEDGLKHMIQAISACPTTIAG
ILEFADRVSKDEMRVDELVDGLLDPSTEEIISEEISDESLEQELNSDAEDEDVTAVANANLLKLKNDALERFAVVQKAYD
EMQKVLEKKGSGNKAYKDIQEQISSELMAIRFSAKMVERLCDTQRALVDEMRGYERKIMELCVSKVGMSRNHFIKTFPGN
ESNLNWVDEEIALGKPYSAALERYRPEIVEQQQNLLALQKQVGIPLKELKEINRRMSTGEAKARRAKREMTEANLRLVIS
IAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQ
ILQETGQEPEPAVLAQKMEMPEEKIRKILKISKEPISMETPIGDDEDSHLGDFIEDSATMAPADAAVYASLRDVTKDILD
SLTPREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSERLRSFLDTEG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=609, Percent_Identity=58.4564860426929, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=237, Percent_Identity=40.5063291139241, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1789871, Length=241, Percent_Identity=27.3858921161826, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 80103; Mature: 79972

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKESEDMKKLLRAQAKEAAAKELTKVDEEMFDNDSETETDSMAKAPEQVTEIAKAVMA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCHHHHHHHHHHHHH
AKEPTLANGKIARSRAAREGKNSHAKDPEKAAASQELEARRMRLKNLIVQGKERGYLTYA
HCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
EINDHLPDDMLDAEQIENIISMINDVGISVYDEAPDAETLLMSETAPTVADEDVVEEAEA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHCCCCCCCCCCHHHHHHHHH
ALSTVDSEFGRTTDPVRMYMREMGSVELLTRESEIEIAKRIEDGLKHMIQAISACPTTIA
HHHHHHHHHCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GILEFADRVSKDEMRVDELVDGLLDPSTEEIISEEISDESLEQELNSDAEDEDVTAVANA
HHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHCC
NLLKLKNDALERFAVVQKAYDEMQKVLEKKGSGNKAYKDIQEQISSELMAIRFSAKMVER
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LCDTQRALVDEMRGYERKIMELCVSKVGMSRNHFIKTFPGNESNLNWVDEEIALGKPYSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHCCCCHHH
ALERYRPEIVEQQQNLLALQKQVGIPLKELKEINRRMSTGEAKARRAKREMTEANLRLVI
HHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
SIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQAR
HHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
TIRIPVHMIETINKMNRISRQILQETGQEPEPAVLAQKMEMPEEKIRKILKISKEPISME
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
TPIGDDEDSHLGDFIEDSATMAPADAAVYASLRDVTKDILDSLTPREAKVLRMRFGIEMN
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
TDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSERLRSFLDTEG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
AIKESEDMKKLLRAQAKEAAAKELTKVDEEMFDNDSETETDSMAKAPEQVTEIAKAVMA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCHHHHHHHHHHHHH
AKEPTLANGKIARSRAAREGKNSHAKDPEKAAASQELEARRMRLKNLIVQGKERGYLTYA
HCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
EINDHLPDDMLDAEQIENIISMINDVGISVYDEAPDAETLLMSETAPTVADEDVVEEAEA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHCCCCCCCCCCHHHHHHHHH
ALSTVDSEFGRTTDPVRMYMREMGSVELLTRESEIEIAKRIEDGLKHMIQAISACPTTIA
HHHHHHHHHCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GILEFADRVSKDEMRVDELVDGLLDPSTEEIISEEISDESLEQELNSDAEDEDVTAVANA
HHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHCC
NLLKLKNDALERFAVVQKAYDEMQKVLEKKGSGNKAYKDIQEQISSELMAIRFSAKMVER
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LCDTQRALVDEMRGYERKIMELCVSKVGMSRNHFIKTFPGNESNLNWVDEEIALGKPYSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHCCCCHHH
ALERYRPEIVEQQQNLLALQKQVGIPLKELKEINRRMSTGEAKARRAKREMTEANLRLVI
HHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
SIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQAR
HHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
TIRIPVHMIETINKMNRISRQILQETGQEPEPAVLAQKMEMPEEKIRKILKISKEPISME
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
TPIGDDEDSHLGDFIEDSATMAPADAAVYASLRDVTKDILDSLTPREAKVLRMRFGIEMN
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
TDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSERLRSFLDTEG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA