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The map label for this gene is rfaF [H]

Identifier: 82702914

GI number: 82702914

Start: 2042221

End: 2043447

Strand: Reverse

Name: rfaF [H]

Synonym: Nmul_A1791

Alternate gene names: 82702914

Gene position: 2043447-2042221 (Counterclockwise)

Preceding gene: 82702915

Following gene: 82702913

Centisome position: 64.17

GC content: 55.83

Gene sequence:

>1227_bases
ATGTTTGCCGGAGCCTGGAAAAATTACAGGAACATATTATGCGTTCGCCCGGACAACATGGGCGATGTGCTGATGAGCCA
GCCGGCGATGCGGGCGCTCAAGGAATCGGTTCCGGGCAGAAAAATCACTCTGTTGACTTCCACCGCGGGAGCATGCATTG
CTCCCTTCATTCCGGAAGTGGACGAAACGATTCCGTTCGATGTTCCCTGGGTAAAGACGACCGAAACCAATGGAGCGCAA
CGGTTGCTGGCGCTTGCCGGCGAATTGCTAGCCCTTCAGCTTGATGCCGCAGTAATCTTTACAGTCTATAGCCAGAACCC
GTTGCCGGCTGGCATGTTGTGCTATCTGGCGGGTATCAAAGCCGTTCTGGGCTATTGCCGCGAGAATCCTTACCAGCTTA
TCAATCAGTGGGTGCCGGACAGGGAACCGCTGGACTACGTCGTCCACGAGGTCGAACGGCAGCTTCGCCTGGTCGAGATG
GCCGGCGCAAAAACCTCCGATACCCGGCTCCTGCTGAAAATTCCAGAGGAAACGCGGAAAGAAGCAACGGACAGGGTATG
GGAGATATTAAATGTAGCCGGCATCCGGGCAGGTATGGGCTGGCTGGTGCTGCATGCGGGCGTCAGTGAAGAAAAGCGTC
TTTATCCGGCCAGAGATTATATAACGGCCTGCCAATCGCTCATCAGGCAAGGCTATAAGATCCTGCTCACAGGCAGCGGC
AGCGAGCGCGATTATGTAGACCAGATCGCTCGCCAGCTCAGTGATGCCGCGATAAATGTCGCAGGCGAGCTATCCATTGC
AGAACTGATTGTCCTGGTCGAAGCGGCACCCGTCTTGATCTCGAATAATACCGGTCCTGTCCATATTGCCACCGCCGTCG
GCACCCCCGTGGTGGTACTGTACGCGATGACAAATCCCCAGCACACGCCATGGCAAGTGCCAAGCAAAGTGCTCTACTTC
GAGGTGCTGCCGGAACTGAGGACAAAGAACCAGCTGCTTCAGTCCTTCCCCGGATCCTCCACACCCAGGGCTTCACCGGA
GGCAATAGTGGCGGCTGTAGGTGAGCTTGTTTCACCACGGAGAAAACACGGATCCTTCATTCCAAGCCCCCTCCGGAATC
CAGTCCCCACCTGCAATAGCCTTCCTGACACTGCACTGGCTGCCTGTTTCACTCCGCATAATGAATATGGCATGTGCCCG
CCTATTCGATATGGCGAGGATGTATGA

Upstream 100 bases:

>100_bases
AAGTGGAAATCATGCCTTTTGTTCAGGACCGATCCACGACAAACATCATATCCAGGATCAAAAGCATGGACAAACAGGTT
GCCTAGGCCGGAAAATCAAA

Downstream 100 bases:

>100_bases
AGCCAGCACAAACCCCGGTTGTATTTATATTGCATCGTTCCGGCTCTGGTCTGCAATCCAGGGCGGAAACGACAACGAAG
CCGGGCTGCTACTGCATTCC

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MFAGAWKNYRNILCVRPDNMGDVLMSQPAMRALKESVPGRKITLLTSTAGACIAPFIPEVDETIPFDVPWVKTTETNGAQ
RLLALAGELLALQLDAAVIFTVYSQNPLPAGMLCYLAGIKAVLGYCRENPYQLINQWVPDREPLDYVVHEVERQLRLVEM
AGAKTSDTRLLLKIPEETRKEATDRVWEILNVAGIRAGMGWLVLHAGVSEEKRLYPARDYITACQSLIRQGYKILLTGSG
SERDYVDQIARQLSDAAINVAGELSIAELIVLVEAAPVLISNNTGPVHIATAVGTPVVVLYAMTNPQHTPWQVPSKVLYF
EVLPELRTKNQLLQSFPGSSTPRASPEAIVAAVGELVSPRRKHGSFIPSPLRNPVPTCNSLPDTALAACFTPHNEYGMCP
PIRYGEDV

Sequences:

>Translated_408_residues
MFAGAWKNYRNILCVRPDNMGDVLMSQPAMRALKESVPGRKITLLTSTAGACIAPFIPEVDETIPFDVPWVKTTETNGAQ
RLLALAGELLALQLDAAVIFTVYSQNPLPAGMLCYLAGIKAVLGYCRENPYQLINQWVPDREPLDYVVHEVERQLRLVEM
AGAKTSDTRLLLKIPEETRKEATDRVWEILNVAGIRAGMGWLVLHAGVSEEKRLYPARDYITACQSLIRQGYKILLTGSG
SERDYVDQIARQLSDAAINVAGELSIAELIVLVEAAPVLISNNTGPVHIATAVGTPVVVLYAMTNPQHTPWQVPSKVLYF
EVLPELRTKNQLLQSFPGSSTPRASPEAIVAAVGELVSPRRKHGSFIPSPLRNPVPTCNSLPDTALAACFTPHNEYGMCP
PIRYGEDV
>Mature_408_residues
MFAGAWKNYRNILCVRPDNMGDVLMSQPAMRALKESVPGRKITLLTSTAGACIAPFIPEVDETIPFDVPWVKTTETNGAQ
RLLALAGELLALQLDAAVIFTVYSQNPLPAGMLCYLAGIKAVLGYCRENPYQLINQWVPDREPLDYVVHEVERQLRLVEM
AGAKTSDTRLLLKIPEETRKEATDRVWEILNVAGIRAGMGWLVLHAGVSEEKRLYPARDYITACQSLIRQGYKILLTGSG
SERDYVDQIARQLSDAAINVAGELSIAELIVLVEAAPVLISNNTGPVHIATAVGTPVVVLYAMTNPQHTPWQVPSKVLYF
EVLPELRTKNQLLQSFPGSSTPRASPEAIVAAVGELVSPRRKHGSFIPSPLRNPVPTCNSLPDTALAACFTPHNEYGMCP
PIRYGEDV

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=312, Percent_Identity=25.3205128205128, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 44736; Mature: 44736

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAGAWKNYRNILCVRPDNMGDVLMSQPAMRALKESVPGRKITLLTSTAGACIAPFIPEV
CCCCCCCCCCCEEEECCCCCCHHHHCCHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCCC
DETIPFDVPWVKTTETNGAQRLLALAGELLALQLDAAVIFTVYSQNPLPAGMLCYLAGIK
CCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHEECCEEEEEEECCCCCCHHHHHHHHHHH
AVLGYCRENPYQLINQWVPDREPLDYVVHEVERQLRLVEMAGAKTSDTRLLLKIPEETRK
HHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHH
EATDRVWEILNVAGIRAGMGWLVLHAGVSEEKRLYPARDYITACQSLIRQGYKILLTGSG
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCCCHHHHHHHHHHHHHCCCEEEEECCC
SERDYVDQIARQLSDAAINVAGELSIAELIVLVEAAPVLISNNTGPVHIATAVGTPVVVL
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCEEEEEECCCCEEEE
YAMTNPQHTPWQVPSKVLYFEVLPELRTKNQLLQSFPGSSTPRASPEAIVAAVGELVSPR
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCH
RKHGSFIPSPLRNPVPTCNSLPDTALAACFTPHNEYGMCPPIRYGEDV
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFAGAWKNYRNILCVRPDNMGDVLMSQPAMRALKESVPGRKITLLTSTAGACIAPFIPEV
CCCCCCCCCCCEEEECCCCCCHHHHCCHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCCC
DETIPFDVPWVKTTETNGAQRLLALAGELLALQLDAAVIFTVYSQNPLPAGMLCYLAGIK
CCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHEECCEEEEEEECCCCCCHHHHHHHHHHH
AVLGYCRENPYQLINQWVPDREPLDYVVHEVERQLRLVEMAGAKTSDTRLLLKIPEETRK
HHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHH
EATDRVWEILNVAGIRAGMGWLVLHAGVSEEKRLYPARDYITACQSLIRQGYKILLTGSG
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCCCHHHHHHHHHHHHHCCCEEEEECCC
SERDYVDQIARQLSDAAINVAGELSIAELIVLVEAAPVLISNNTGPVHIATAVGTPVVVL
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCEEEEEECCCCEEEE
YAMTNPQHTPWQVPSKVLYFEVLPELRTKNQLLQSFPGSSTPRASPEAIVAAVGELVSPR
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCH
RKHGSFIPSPLRNPVPTCNSLPDTALAACFTPHNEYGMCPPIRYGEDV
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]