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The map label for this gene is hldE [H]

Identifier: 82702915

GI number: 82702915

Start: 2043462

End: 2044937

Strand: Reverse

Name: hldE [H]

Synonym: Nmul_A1792

Alternate gene names: 82702915

Gene position: 2044937-2043462 (Counterclockwise)

Preceding gene: 82702916

Following gene: 82702914

Centisome position: 64.22

GC content: 53.46

Gene sequence:

>1476_bases
ATGAACAGCAGTTATCTTGCCACTATCGATCGCTTTAGCGGTTTGAATGTACTTGTAATCGGCGATGCGATGCTCGATGT
TTATCTCGACGGGGCGGCAAACCGTATCTGCAGGGAAGCGCCTGTTCCCATCGTGGATATCAGGGATGTCAAGGCAGTGC
CGGGAGGAGCGGCTAATACGGCAGTAAATCTGGCACAACTGGGTGCCACAGTCCACTATCTTTCGGTTGTCGGATGTGAT
CACGAGGCCGAGTTGCTGAAAGAATCGCTCGTCAGTCATGGCCTCGACATTTCCCTGGTTATCTGCGATCCGGCCCGTCG
AACGCTTACCAAACAGCGTGTCGCCGCAGGTACCCAACTGCTGGTGCGTTTTGATGCCGGCACGACAGAGCGGGTATGCA
ACAAGCACGAGCAGCAGATGATCGATACCCTGAACGAAAAGTTTCATGAGATGGATGCTGTCGTTGTTTCCGATTATGGT
TATGGCATTCTCACGGACCCAGTGATCGATGCTCTGCGTGCTTTGCAGAAAAAAAAGGAAAACACTCTGGTAATCGACGC
AAAATCGCTCGATAAATACAGCCGGGTTGGGGCGACCGTGGCCAAGCCGAACTATCAGGAACTCGTCACGCTGCTTGCCA
TCACGGAACCGGCGCCTGTGGGCCAGCGTTCGGAGCAGATCAGGCTCTACGGACAAAAGCTGCTCAAAAAAACCGGAACG
AAATATGTTGCGGCTACCATCGATGTCGAAGGCGCATTGCTGTTTCAGCACGGCAAGGAGCCTTATCGCACCTTCTCCAA
ACCGGTAGAGAACACCCGGGCAGCCGGAGCAGGGGATACCTATGCAGGCGCGCTCACGCTGGCTCTCGCCAGCGGATCAT
CTATTGAGGTGGCGGGTGAGATTGCCAAGTCTGCCGCGATGGTGATACTTCAAAAATCCCACACCGCCACCTGCACTCAG
AACGAACTGCGGCATTACCTGGGAAGCAGCAGTAAATATGTCGCGGACTGGCAAGCCCTGGATAATCGTCTGGATGCGTT
CAGAAAAGAAGGCAAGCGCATCGTGTTTACCAACGGGTGCTTCGACCTCCTTCATAGCGGCCATGTCAGCTATCTCGAGC
AAGCACGGGCTCTGGGTGACGTTCTGGTGCTCGGCCTCAATTCCGATGCGAGTATAAAACGTCTGAAGGGGAGCAATCGC
CCCATCAACAACCTGTATGAACGTATCCGCATCCTCTCGGGATTGGAGTCGGTAACACTCATGACGTCATTCGAAGAAGA
CACGCCGGTCAATCTTCTCGAAGTCATTCAGCCAAGTTTGTATGTAAAAGGCGGTGACTATACGATCGAAACGCTTCCCG
AAGCGCCGGTTGTCCGGGCATACGGCGGAAAAGTGGAAATCATGCCTTTTGTTCAGGACCGATCCACGACAAACATCATA
TCCAGGATCAAAAGCATGGACAAACAGGTTGCCTAG

Upstream 100 bases:

>100_bases
GCACACTGCTGATACTGGCAGTTTTCCCGATTGCTCCCTGGAATTTGCTTTGACCCCGTATAGCTTGCAGGGCGACATAT
TCCAGGTAAAAGGAGGCAGC

Downstream 100 bases:

>100_bases
GCCGGAAAATCAAAATGTTTGCCGGAGCCTGGAAAAATTACAGGAACATATTATGCGTTCGCCCGGACAACATGGGCGAT
GTGCTGATGAGCCAGCCGGC

Product: bifunctional ADP-heptose synthase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MNSSYLATIDRFSGLNVLVIGDAMLDVYLDGAANRICREAPVPIVDIRDVKAVPGGAANTAVNLAQLGATVHYLSVVGCD
HEAELLKESLVSHGLDISLVICDPARRTLTKQRVAAGTQLLVRFDAGTTERVCNKHEQQMIDTLNEKFHEMDAVVVSDYG
YGILTDPVIDALRALQKKKENTLVIDAKSLDKYSRVGATVAKPNYQELVTLLAITEPAPVGQRSEQIRLYGQKLLKKTGT
KYVAATIDVEGALLFQHGKEPYRTFSKPVENTRAAGAGDTYAGALTLALASGSSIEVAGEIAKSAAMVILQKSHTATCTQ
NELRHYLGSSSKYVADWQALDNRLDAFRKEGKRIVFTNGCFDLLHSGHVSYLEQARALGDVLVLGLNSDASIKRLKGSNR
PINNLYERIRILSGLESVTLMTSFEEDTPVNLLEVIQPSLYVKGGDYTIETLPEAPVVRAYGGKVEIMPFVQDRSTTNII
SRIKSMDKQVA

Sequences:

>Translated_491_residues
MNSSYLATIDRFSGLNVLVIGDAMLDVYLDGAANRICREAPVPIVDIRDVKAVPGGAANTAVNLAQLGATVHYLSVVGCD
HEAELLKESLVSHGLDISLVICDPARRTLTKQRVAAGTQLLVRFDAGTTERVCNKHEQQMIDTLNEKFHEMDAVVVSDYG
YGILTDPVIDALRALQKKKENTLVIDAKSLDKYSRVGATVAKPNYQELVTLLAITEPAPVGQRSEQIRLYGQKLLKKTGT
KYVAATIDVEGALLFQHGKEPYRTFSKPVENTRAAGAGDTYAGALTLALASGSSIEVAGEIAKSAAMVILQKSHTATCTQ
NELRHYLGSSSKYVADWQALDNRLDAFRKEGKRIVFTNGCFDLLHSGHVSYLEQARALGDVLVLGLNSDASIKRLKGSNR
PINNLYERIRILSGLESVTLMTSFEEDTPVNLLEVIQPSLYVKGGDYTIETLPEAPVVRAYGGKVEIMPFVQDRSTTNII
SRIKSMDKQVA
>Mature_491_residues
MNSSYLATIDRFSGLNVLVIGDAMLDVYLDGAANRICREAPVPIVDIRDVKAVPGGAANTAVNLAQLGATVHYLSVVGCD
HEAELLKESLVSHGLDISLVICDPARRTLTKQRVAAGTQLLVRFDAGTTERVCNKHEQQMIDTLNEKFHEMDAVVVSDYG
YGILTDPVIDALRALQKKKENTLVIDAKSLDKYSRVGATVAKPNYQELVTLLAITEPAPVGQRSEQIRLYGQKLLKKTGT
KYVAATIDVEGALLFQHGKEPYRTFSKPVENTRAAGAGDTYAGALTLALASGSSIEVAGEIAKSAAMVILQKSHTATCTQ
NELRHYLGSSSKYVADWQALDNRLDAFRKEGKRIVFTNGCFDLLHSGHVSYLEQARALGDVLVLGLNSDASIKRLKGSNR
PINNLYERIRILSGLESVTLMTSFEEDTPVNLLEVIQPSLYVKGGDYTIETLPEAPVVRAYGGKVEIMPFVQDRSTTNII
SRIKSMDKQVA

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=488, Percent_Identity=37.9098360655738, Blast_Score=281, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI193203447, Length=61, Percent_Identity=42.6229508196721, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17509873, Length=61, Percent_Identity=42.6229508196721, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921220, Length=59, Percent_Identity=50.8474576271186, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45550975, Length=59, Percent_Identity=50.8474576271186, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45550973, Length=59, Percent_Identity=50.8474576271186, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45550974, Length=59, Percent_Identity=50.8474576271186, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 53512; Mature: 53512

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSYLATIDRFSGLNVLVIGDAMLDVYLDGAANRICREAPVPIVDIRDVKAVPGGAANT
CCCCEEEEHHHCCCCEEEEEECCEEEHEECCHHHHHHHHCCCCEEECCCCCCCCCCCCHH
AVNLAQLGATVHYLSVVGCDHEAELLKESLVSHGLDISLVICDPARRTLTKQRVAAGTQL
HHHHHHHHHHHEEHHEECCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCEE
LVRFDAGTTERVCNKHEQQMIDTLNEKFHEMDAVVVSDYGYGILTDPVIDALRALQKKKE
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHCCC
NTLVIDAKSLDKYSRVGATVAKPNYQELVTLLAITEPAPVGQRSEQIRLYGQKLLKKTGT
CEEEEEHHHHHHHHHCCCEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
KYVAATIDVEGALLFQHGKEPYRTFSKPVENTRAAGAGDTYAGALTLALASGSSIEVAGE
EEEEEEEECCCEEEECCCCCHHHHHHCCHHHCCCCCCCCCHHHEEEEEEECCCCEEHHHH
IAKSAAMVILQKSHTATCTQNELRHYLGSSSKYVADWQALDNRLDAFRKEGKRIVFTNGC
HHHHHEEEEEECCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCEEEEECCH
FDLLHSGHVSYLEQARALGDVLVLGLNSDASIKRLKGSNRPINNLYERIRILSGLESVTL
HHHHHCCCHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCEEE
MTSFEEDTPVNLLEVIQPSLYVKGGDYTIETLPEAPVVRAYGGKVEIMPFVQDRSTTNII
EECCCCCCCCHHHHHCCCCEEEECCCEEEEECCCCCEEEECCCEEEEEEECCCCCHHHHH
SRIKSMDKQVA
HHHHHHHHCCC
>Mature Secondary Structure
MNSSYLATIDRFSGLNVLVIGDAMLDVYLDGAANRICREAPVPIVDIRDVKAVPGGAANT
CCCCEEEEHHHCCCCEEEEEECCEEEHEECCHHHHHHHHCCCCEEECCCCCCCCCCCCHH
AVNLAQLGATVHYLSVVGCDHEAELLKESLVSHGLDISLVICDPARRTLTKQRVAAGTQL
HHHHHHHHHHHEEHHEECCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCEE
LVRFDAGTTERVCNKHEQQMIDTLNEKFHEMDAVVVSDYGYGILTDPVIDALRALQKKKE
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHCCC
NTLVIDAKSLDKYSRVGATVAKPNYQELVTLLAITEPAPVGQRSEQIRLYGQKLLKKTGT
CEEEEEHHHHHHHHHCCCEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
KYVAATIDVEGALLFQHGKEPYRTFSKPVENTRAAGAGDTYAGALTLALASGSSIEVAGE
EEEEEEEECCCEEEECCCCCHHHHHHCCHHHCCCCCCCCCHHHEEEEEEECCCCEEHHHH
IAKSAAMVILQKSHTATCTQNELRHYLGSSSKYVADWQALDNRLDAFRKEGKRIVFTNGC
HHHHHEEEEEECCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCEEEEECCH
FDLLHSGHVSYLEQARALGDVLVLGLNSDASIKRLKGSNRPINNLYERIRILSGLESVTL
HHHHHCCCHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCEEE
MTSFEEDTPVNLLEVIQPSLYVKGGDYTIETLPEAPVVRAYGGKVEIMPFVQDRSTTNII
EECCCCCCCCHHHHHCCCCEEEECCCEEEEECCCCCEEEECCCEEEEEEECCCCCHHHHH
SRIKSMDKQVA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA