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The map label for this gene is rfaF [H]

Identifier: 82702906

GI number: 82702906

Start: 2036280

End: 2037287

Strand: Reverse

Name: rfaF [H]

Synonym: Nmul_A1783

Alternate gene names: 82702906

Gene position: 2037287-2036280 (Counterclockwise)

Preceding gene: 82702909

Following gene: 82702905

Centisome position: 63.98

GC content: 55.95

Gene sequence:

>1008_bases
ATGGCTCAGATGGGTAATCTGCCAATCAAGAAGATCGCGATTTTCAGGGCCTTGAAGCTGGGGGATTTTCTGTTGTTCGT
CCCTGCGCTGCGGGCGATTAGGCGGGCTTTTCCGCAAGCCACCATCGATTATGTCGGGTTGCCCTGGAACAAGGCGCTCG
CCGCGCGTTACAATCATTATATCGACGAATTTATCGAATTTCCGGGTTTTCCCGGATTGCCCGAGCATCCCTTCAGGGCT
GAAGCAGTCACGGCTTTTCTGGACGGCATGCAGCGGCGACAATACGACCTTGCCCTGCAAATGCATGGCAAAGGTACAGT
ATCTAATCTTGTCGTCTCTCTTTTCGGGGCGGCTATTGCGGCCGGGTTTGCGAGCGAAGGCAACTCTCACTGGCCTAACC
GCGATTTCTTCATGCCATACCCCTCCAGGCAGCCTGAGTTGCTAAGGAATCTGGCGTTACTCGAGTTTCTCGGCATGGAG
CAGGCTGATCGCGCGGCGGACAGAACCATGGAATTTCCGTTATTGGACATGGACTGCCAGAAACTTCGGGAACTGCAAGA
ATATGGGACTATTCGTGATAAACCTTATGTCTGCCTGCATCCCGGCGCGATTTCCGCAACCCCCTGGCCTGCTGCTCATT
TCGCGGAGGTGGCGGACAGGTGTATTCGGCAGGGTTTGAAAGTGGTGTTGACGGGTACTGCGGAAGAGAAGCCGCTCACG
CAAGCGGTCGCCGGGAAAATGACGGGTACGGCGATTGATCTTGCCGGTAAAACCGCCATCGGGGCACTCGCCGCCCTTCT
GAAGGGCAGCCGGGCGGTGATCTCGAATGACACGGGAGTTGCGCATTTGGCGGTAGCGGTCGATGCCCCGAGTGTCACCG
TCTTTACCACGACCGATCCGCTGATTTGGGGTCCGTTGGATCAGGTTCATCATCGGGTTGTTTCGGGAAACGACGTGAAG
ACGCCGGAAATGGCAATACGGGCGCTGGAGGAATTAATTGGGCGTTAA

Upstream 100 bases:

>100_bases
AGGAGAATTGGTTATAAAAATAGCGGAACCCTGGCTTACATGCAATACTGTTGAGCGAAGAAAGTCTGACTGACTTGACT
CGTACCGGGAGCGCAAGCAT

Downstream 100 bases:

>100_bases
TATGAAAGTAAAGGAGAATCAAGGAAGAAAGGAGGAGAAATTGCGGGTTCTTACCTGGCATATTCATGGCAACTACCTTT
ACTATCTCACTCAGGCGCCG

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAQMGNLPIKKIAIFRALKLGDFLLFVPALRAIRRAFPQATIDYVGLPWNKALAARYNHYIDEFIEFPGFPGLPEHPFRA
EAVTAFLDGMQRRQYDLALQMHGKGTVSNLVVSLFGAAIAAGFASEGNSHWPNRDFFMPYPSRQPELLRNLALLEFLGME
QADRAADRTMEFPLLDMDCQKLRELQEYGTIRDKPYVCLHPGAISATPWPAAHFAEVADRCIRQGLKVVLTGTAEEKPLT
QAVAGKMTGTAIDLAGKTAIGALAALLKGSRAVISNDTGVAHLAVAVDAPSVTVFTTTDPLIWGPLDQVHHRVVSGNDVK
TPEMAIRALEELIGR

Sequences:

>Translated_335_residues
MAQMGNLPIKKIAIFRALKLGDFLLFVPALRAIRRAFPQATIDYVGLPWNKALAARYNHYIDEFIEFPGFPGLPEHPFRA
EAVTAFLDGMQRRQYDLALQMHGKGTVSNLVVSLFGAAIAAGFASEGNSHWPNRDFFMPYPSRQPELLRNLALLEFLGME
QADRAADRTMEFPLLDMDCQKLRELQEYGTIRDKPYVCLHPGAISATPWPAAHFAEVADRCIRQGLKVVLTGTAEEKPLT
QAVAGKMTGTAIDLAGKTAIGALAALLKGSRAVISNDTGVAHLAVAVDAPSVTVFTTTDPLIWGPLDQVHHRVVSGNDVK
TPEMAIRALEELIGR
>Mature_334_residues
AQMGNLPIKKIAIFRALKLGDFLLFVPALRAIRRAFPQATIDYVGLPWNKALAARYNHYIDEFIEFPGFPGLPEHPFRAE
AVTAFLDGMQRRQYDLALQMHGKGTVSNLVVSLFGAAIAAGFASEGNSHWPNRDFFMPYPSRQPELLRNLALLEFLGMEQ
ADRAADRTMEFPLLDMDCQKLRELQEYGTIRDKPYVCLHPGAISATPWPAAHFAEVADRCIRQGLKVVLTGTAEEKPLTQ
AVAGKMTGTAIDLAGKTAIGALAALLKGSRAVISNDTGVAHLAVAVDAPSVTVFTTTDPLIWGPLDQVHHRVVSGNDVKT
PEMAIRALEELIGR

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790062, Length=314, Percent_Identity=27.0700636942675, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790050, Length=344, Percent_Identity=23.2558139534884, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 36685; Mature: 36554

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQMGNLPIKKIAIFRALKLGDFLLFVPALRAIRRAFPQATIDYVGLPWNKALAARYNHY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCCHHHHHHHHHHHH
IDEFIEFPGFPGLPEHPFRAEAVTAFLDGMQRRQYDLALQMHGKGTVSNLVVSLFGAAIA
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHH
AGFASEGNSHWPNRDFFMPYPSRQPELLRNLALLEFLGMEQADRAADRTMEFPLLDMDCQ
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHH
KLRELQEYGTIRDKPYVCLHPGAISATPWPAAHFAEVADRCIRQGLKVVLTGTAEEKPLT
HHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
QAVAGKMTGTAIDLAGKTAIGALAALLKGSRAVISNDTGVAHLAVAVDAPSVTVFTTTDP
HHHHCCCCCCEEECCCHHHHHHHHHHHCCCCEEEECCCCEEEEEEEECCCCEEEEECCCC
LIWGPLDQVHHRVVSGNDVKTPEMAIRALEELIGR
CEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQMGNLPIKKIAIFRALKLGDFLLFVPALRAIRRAFPQATIDYVGLPWNKALAARYNHY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCCHHHHHHHHHHHH
IDEFIEFPGFPGLPEHPFRAEAVTAFLDGMQRRQYDLALQMHGKGTVSNLVVSLFGAAIA
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHH
AGFASEGNSHWPNRDFFMPYPSRQPELLRNLALLEFLGMEQADRAADRTMEFPLLDMDCQ
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHH
KLRELQEYGTIRDKPYVCLHPGAISATPWPAAHFAEVADRCIRQGLKVVLTGTAEEKPLT
HHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
QAVAGKMTGTAIDLAGKTAIGALAALLKGSRAVISNDTGVAHLAVAVDAPSVTVFTTTDP
HHHHCCCCCCEEECCCHHHHHHHHHHHCCCCEEEECCCCEEEEEEEECCCCEEEEECCCC
LIWGPLDQVHHRVVSGNDVKTPEMAIRALEELIGR
CEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Transferring other glycosyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]