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The map label for this gene is mcrA [H]

Identifier: 82702726

GI number: 82702726

Start: 1833075

End: 1833755

Strand: Reverse

Name: mcrA [H]

Synonym: Nmul_A1599

Alternate gene names: 82702726

Gene position: 1833755-1833075 (Counterclockwise)

Preceding gene: 82702728

Following gene: 82702725

Centisome position: 57.59

GC content: 53.16

Gene sequence:

>681_bases
ATGGATGACGTTTCCTGGAGCGATGAAGAACTGAAGGCTTCCATTGAAGCTTACGTTGAGATGCAGCGCAATGAGCGTAC
GGGCCAGCTTATTGTAAAGAAGCAGTATTACAAAAAGCTGGTGGAAATGTTCGGCCGCAGCGAACGGGTTTTTGAATCTC
TCATGCAGAACATTTCCTATGTCCTTTCGCTCATGGGACGCGGATGGATGAGCGGACTGAAGCCGGCAAGAGATATCGGG
CCTCTTGTTGCTGCGCGGATCGAACAATTGCTGGAGCAGATCAGTGGACAAAAGGCGGTTCCAGTAGCCGCTTTTGAGAT
TGCTGTGCGCGAGGCAGCGGAACAGAAAGACCTTCCCAAGCCCAGCGGTAATCCCCGCCCTAAACGCCGACGCATATCTG
TTGCGCAATTAGAGCGTGATCCCAACGTGAAGGCATGGGTGTTGCAACAGGCAGCAGGAACTTGCGAATCATGCGAAAAA
CCCGCTCCATTTCAAGGAGCGGATGGATCACCTTATCTCGAATTGCATTACGTTCAGGGCCTGGCTGATGGCGGAGCCGA
TGCTGTCTCCAATGCAGTGGCGCTGTGTCCCAATTGTCATCGCGAGATACATTATGGCGCCAATGCTCAGGCTGTGGAAG
CGTGGCTGTATGACACGGTCCAGAGACTGGAGCGGGATTAG

Upstream 100 bases:

>100_bases
AGCTTCTGTGCGGTGACGCACTTATTGCAGAGGTCGAATTTGCACAGGTGTGAGCGCCGAGTCATAATCCTGACTGTGGC
TCGTTAATTGGCAGAAAAGC

Downstream 100 bases:

>100_bases
ATAAGGTAAAGGTCGGGTCGAAATCATGAATCCGGGAAAGAGCTGATGCTCTGGCAGGCAATGACCGCAGCGCGGGATCT
CGGGCGCCTGCACGAGATTG

Product: HNH nuclease

Products: NA

Alternate protein names: EcoKMcrA [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MDDVSWSDEELKASIEAYVEMQRNERTGQLIVKKQYYKKLVEMFGRSERVFESLMQNISYVLSLMGRGWMSGLKPARDIG
PLVAARIEQLLEQISGQKAVPVAAFEIAVREAAEQKDLPKPSGNPRPKRRRISVAQLERDPNVKAWVLQQAAGTCESCEK
PAPFQGADGSPYLELHYVQGLADGGADAVSNAVALCPNCHREIHYGANAQAVEAWLYDTVQRLERD

Sequences:

>Translated_226_residues
MDDVSWSDEELKASIEAYVEMQRNERTGQLIVKKQYYKKLVEMFGRSERVFESLMQNISYVLSLMGRGWMSGLKPARDIG
PLVAARIEQLLEQISGQKAVPVAAFEIAVREAAEQKDLPKPSGNPRPKRRRISVAQLERDPNVKAWVLQQAAGTCESCEK
PAPFQGADGSPYLELHYVQGLADGGADAVSNAVALCPNCHREIHYGANAQAVEAWLYDTVQRLERD
>Mature_226_residues
MDDVSWSDEELKASIEAYVEMQRNERTGQLIVKKQYYKKLVEMFGRSERVFESLMQNISYVLSLMGRGWMSGLKPARDIG
PLVAARIEQLLEQISGQKAVPVAAFEIAVREAAEQKDLPKPSGNPRPKRRRISVAQLERDPNVKAWVLQQAAGTCESCEK
PAPFQGADGSPYLELHYVQGLADGGADAVSNAVALCPNCHREIHYGANAQAVEAWLYDTVQRLERD

Specific function: Restriction of 5-methyl and 5-hydroxymethylcytosines at the specific DNA sequence C(me)CGG [H]

COG id: COG1403

COG function: function code V; Restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787406, Length=115, Percent_Identity=49.5652173913044, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002711
- InterPro:   IPR003615 [H]

Pfam domain/function: PF01844 HNH [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 25219; Mature: 25219

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDVSWSDEELKASIEAYVEMQRNERTGQLIVKKQYYKKLVEMFGRSERVFESLMQNISY
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VLSLMGRGWMSGLKPARDIGPLVAARIEQLLEQISGQKAVPVAAFEIAVREAAEQKDLPK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
PSGNPRPKRRRISVAQLERDPNVKAWVLQQAAGTCESCEKPAPFQGADGSPYLELHYVQG
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCEEEEHHHHH
LADGGADAVSNAVALCPNCHREIHYGANAQAVEAWLYDTVQRLERD
HCCCCHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDDVSWSDEELKASIEAYVEMQRNERTGQLIVKKQYYKKLVEMFGRSERVFESLMQNISY
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VLSLMGRGWMSGLKPARDIGPLVAARIEQLLEQISGQKAVPVAAFEIAVREAAEQKDLPK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
PSGNPRPKRRRISVAQLERDPNVKAWVLQQAAGTCESCEKPAPFQGADGSPYLELHYVQG
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCEEEEHHHHH
LADGGADAVSNAVALCPNCHREIHYGANAQAVEAWLYDTVQRLERD
HCCCCHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1938927; 8905232; 9278503 [H]