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The map label for this gene is insB [H]

Identifier: 82544827

GI number: 82544827

Start: 2375506

End: 2375682

Strand: Reverse

Name: insB [H]

Synonym: SBO_2384

Alternate gene names: 82544827

Gene position: 2375682-2375506 (Counterclockwise)

Preceding gene: 161984895

Following gene: 82544824

Centisome position: 52.56

GC content: 45.2

Gene sequence:

>177_bases
GTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCT
GGCAAGGCTGGGACGGAAGTCACTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAGGTCATCGGGCATTATCTGAACA
TAAAACACTATCAGTAA

Upstream 100 bases:

>100_bases
GCAGAGGCGTTCGAACATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCA
GCGGGCTTGTAATGGGTTAA

Downstream 100 bases:

>100_bases
GTTGGAGTCATTACCCAAACCACGCTGAAGCACTCCTGGCGTTTTCACTACAATATTGGGTGAGACTATCACCATTAATA
CGAATTCCTCTTTGAATGTG

Product: IS1 ORF2

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 58; Mature: 58

Protein sequence:

>58_residues
MITDVWKYRGKSTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Sequences:

>Translated_58_residues
MITDVWKYRGKSTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
>Mature_58_residues
MITDVWKYRGKSTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=46, Percent_Identity=100, Blast_Score=95, Evalue=8e-22,
Organism=Escherichia coli, GI1788203, Length=46, Percent_Identity=100, Blast_Score=95, Evalue=8e-22,
Organism=Escherichia coli, GI1786203, Length=46, Percent_Identity=100, Blast_Score=95, Evalue=8e-22,
Organism=Escherichia coli, GI1787223, Length=46, Percent_Identity=97.8260869565217, Blast_Score=93, Evalue=3e-21,
Organism=Escherichia coli, GI1786470, Length=46, Percent_Identity=97.8260869565217, Blast_Score=92, Evalue=5e-21,
Organism=Escherichia coli, GI1786459, Length=46, Percent_Identity=97.8260869565217, Blast_Score=92, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 7001; Mature: 7001

Theoretical pI: Translated: 11.11; Mature: 11.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITDVWKYRGKSTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
CCCCCCHHCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MITDVWKYRGKSTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
CCCCCCHHCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]