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The map label for this gene is proB [H]

Identifier: 78779180

GI number: 78779180

Start: 741934

End: 743016

Strand: Reverse

Name: proB [H]

Synonym: PMT9312_0796

Alternate gene names: 78779180

Gene position: 743016-741934 (Counterclockwise)

Preceding gene: 78779181

Following gene: 78779179

Centisome position: 43.47

GC content: 29.82

Gene sequence:

>1083_bases
ATGAAAACTTGGGTTATAAAAATTGGTACTAGTATTTTAAGAGGGACAGAGGAAACATCTACTGAAGAAGTTATTGAAAC
CCTTTGTAGATCCTTTACAAGTTTTCTTTTAAAAGGAAACAAATTGATTTTAGTGACTAGTGGAGCCGTTGGATTAGGTT
GCCAAAAATTAAATATTAAAACTAGGCCAAATGATTTAAGTACCCTTCAAGCTACCGCTGCAGTAGGTCAAGTTAATTTA
ATGTCTTTGTACGATAAAATATTTAATAAATTAGGTCATAATATTGCTCAAATTTTAATCACGAAAGCTGACTTTAATTC
GCGAGAATCTTTCAATAACGCTTCTAAAACATTAAAAAAATTAATCGATTTGAATGTTATTCCAATAGTAAATGAAAATG
ATACCGTAGCAAATGAGGAGCTTAAATATGGAGATAATGATACCCTCTCTGCCTTAGTTGCCTTAGCTATTAATGCTAAT
AAGCTTATTTTATTAACCGACATTGAAAATCTATATTCAAAAGATCCACGTAAGAATAAAGATGCGCATCCTATTAAAGA
GGTTCATAATAGTGAATTAAAAGAAATTAAAAATAAAAACAATCAAAACTCAAATAATGAATGGGGAACAGGAGGAATTT
CTACAAAACTAATTTCTGCAGAAATAGCTACAAAAGGGGGAGTTGAAGTTCAATTAGTTGATGGAACGAATAAAAAAAAT
TTAATTGAGATTTTTAATGATAATAAAATTGGAACTTTATTTTATCCATTAGAAAAACCTATAGGAAACAAAAAAAGTTG
GCTTTCTCATGCAATTCACACAGTAGGAAAAATCACTTTAGATGATGGAGCTTTTTTTGCAATTAACAAAAAAGGTGCCT
CACTTTTAGCGGTTGGTGTTAAAAATGTAGAAGGAAACTTTACGGTTAATCAAGCTGTTAAAATCGTAAATACAGATAAT
AAAGAAGTTGCAAAAGGTTTAGTATCAATAAGTAGTGACAACTTAAGAAGTATCTTAAATAATAAAGACAATAACAACTC
CTCTATAATTGTCGTACACAGAGATGTTCTAGCTCTCTCTTAG

Upstream 100 bases:

>100_bases
ATATAAAAACAGTATTAGTTAAGCGATTAAAGAGAGATGGCTTGCCTGTTAAATTTAATTTAACTTTGACAATAGAAAAA
CTAATTTCTTTTTTTATAAA

Downstream 100 bases:

>100_bases
AAAAAAATTAAAACTCAAAAATGCTTTTAAGTGATTTAGTTGATCTAATTAAAAAAGGAAATTCAAATTTTATTAGTTCC
AATATTTTTGAAGATTTAAA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKTWVIKIGTSILRGTEETSTEEVIETLCRSFTSFLLKGNKLILVTSGAVGLGCQKLNIKTRPNDLSTLQATAAVGQVNL
MSLYDKIFNKLGHNIAQILITKADFNSRESFNNASKTLKKLIDLNVIPIVNENDTVANEELKYGDNDTLSALVALAINAN
KLILLTDIENLYSKDPRKNKDAHPIKEVHNSELKEIKNKNNQNSNNEWGTGGISTKLISAEIATKGGVEVQLVDGTNKKN
LIEIFNDNKIGTLFYPLEKPIGNKKSWLSHAIHTVGKITLDDGAFFAINKKGASLLAVGVKNVEGNFTVNQAVKIVNTDN
KEVAKGLVSISSDNLRSILNNKDNNNSSIIVVHRDVLALS

Sequences:

>Translated_360_residues
MKTWVIKIGTSILRGTEETSTEEVIETLCRSFTSFLLKGNKLILVTSGAVGLGCQKLNIKTRPNDLSTLQATAAVGQVNL
MSLYDKIFNKLGHNIAQILITKADFNSRESFNNASKTLKKLIDLNVIPIVNENDTVANEELKYGDNDTLSALVALAINAN
KLILLTDIENLYSKDPRKNKDAHPIKEVHNSELKEIKNKNNQNSNNEWGTGGISTKLISAEIATKGGVEVQLVDGTNKKN
LIEIFNDNKIGTLFYPLEKPIGNKKSWLSHAIHTVGKITLDDGAFFAINKKGASLLAVGVKNVEGNFTVNQAVKIVNTDN
KEVAKGLVSISSDNLRSILNNKDNNNSSIIVVHRDVLALS
>Mature_360_residues
MKTWVIKIGTSILRGTEETSTEEVIETLCRSFTSFLLKGNKLILVTSGAVGLGCQKLNIKTRPNDLSTLQATAAVGQVNL
MSLYDKIFNKLGHNIAQILITKADFNSRESFNNASKTLKKLIDLNVIPIVNENDTVANEELKYGDNDTLSALVALAINAN
KLILLTDIENLYSKDPRKNKDAHPIKEVHNSELKEIKNKNNQNSNNEWGTGGISTKLISAEIATKGGVEVQLVDGTNKKN
LIEIFNDNKIGTLFYPLEKPIGNKKSWLSHAIHTVGKITLDDGAFFAINKKGASLLAVGVKNVEGNFTVNQAVKIVNTDN
KEVAKGLVSISSDNLRSILNNKDNNNSSIIVVHRDVLALS

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=299, Percent_Identity=29.4314381270903, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI21361368, Length=301, Percent_Identity=29.2358803986711, Blast_Score=115, Evalue=9e-26,
Organism=Escherichia coli, GI1786437, Length=363, Percent_Identity=37.465564738292, Blast_Score=255, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17569967, Length=304, Percent_Identity=27.6315789473684, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17569969, Length=304, Percent_Identity=27.6315789473684, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6320506, Length=355, Percent_Identity=32.6760563380282, Blast_Score=184, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6321822, Length=349, Percent_Identity=34.3839541547278, Blast_Score=172, Evalue=6e-44,
Organism=Drosophila melanogaster, GI21357643, Length=298, Percent_Identity=32.8859060402685, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39331; Mature: 39331

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTWVIKIGTSILRGTEETSTEEVIETLCRSFTSFLLKGNKLILVTSGAVGLGCQKLNIK
CCEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEE
TRPNDLSTLQATAAVGQVNLMSLYDKIFNKLGHNIAQILITKADFNSRESFNNASKTLKK
ECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHEEHEEEECCCCCCHHHHHHHHHHHH
LIDLNVIPIVNENDTVANEELKYGDNDTLSALVALAINANKLILLTDIENLYSKDPRKNK
HHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHEECCCEEEEEECCHHHHCCCCCCCC
DAHPIKEVHNSELKEIKNKNNQNSNNEWGTGGISTKLISAEIATKGGVEVQLVDGTNKKN
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHCCCCCCEEEEECCCCCCC
LIEIFNDNKIGTLFYPLEKPIGNKKSWLSHAIHTVGKITLDDGAFFAINKKGASLLAVGV
EEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCEEEECCCEEEEEECCCCEEEEEEE
KNVEGNFTVNQAVKIVNTDNKEVAKGLVSISSDNLRSILNNKDNNNSSIIVVHRDVLALS
ECCCCCEEHHHEEEEEECCCHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEECCEEECC
>Mature Secondary Structure
MKTWVIKIGTSILRGTEETSTEEVIETLCRSFTSFLLKGNKLILVTSGAVGLGCQKLNIK
CCEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEE
TRPNDLSTLQATAAVGQVNLMSLYDKIFNKLGHNIAQILITKADFNSRESFNNASKTLKK
ECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHEEHEEEECCCCCCHHHHHHHHHHHH
LIDLNVIPIVNENDTVANEELKYGDNDTLSALVALAINANKLILLTDIENLYSKDPRKNK
HHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHEECCCEEEEEECCHHHHCCCCCCCC
DAHPIKEVHNSELKEIKNKNNQNSNNEWGTGGISTKLISAEIATKGGVEVQLVDGTNKKN
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHCCCCCCEEEEECCCCCCC
LIEIFNDNKIGTLFYPLEKPIGNKKSWLSHAIHTVGKITLDDGAFFAINKKGASLLAVGV
EEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCEEEECCCEEEEEECCCCEEEEEEE
KNVEGNFTVNQAVKIVNTDNKEVAKGLVSISSDNLRSILNNKDNNNSSIIVVHRDVLALS
ECCCCCEEHHHEEEEEECCCHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA