Click here to switch to the map view.

The map label for this gene is pstS [H]

Identifier: 78779106

GI number: 78779106

Start: 672671

End: 673666

Strand: Direct

Name: pstS [H]

Synonym: PMT9312_0722

Alternate gene names: 78779106

Gene position: 672671-673666 (Clockwise)

Preceding gene: 78779102

Following gene: 78779110

Centisome position: 39.36

GC content: 38.45

Gene sequence:

>996_bases
ATGACATCCATGAACATAGCTAAGAAAGCTTTGGTTTTCACTTCTGCAGTAGCCATTGCTGCTGGTACAAGTGTTCCTGG
CACAAGTGTTTCTGCTAAAACAAGATTAAGTGGTGCTGGAGCATCATTTCCTGCCAAAATTTACACTCGTTGGTTCAAAG
ATTTAGCTACTTCAGGGGGGCCAAGAGTAAACTATCAAGCTGTTGGTTCAGGCTCTGGAAGAAAAGCTTTTATTGATCAG
ACAGTAAACTTTGGTGCTTCTGATGATCCTATGAAGGATAAAGATATAGCAAAGGTAACAAGAGGATTAGTACAGATTCC
TATGGTTGGAGGAACTATTGCATTTGGTTATAACTATGATTGTGATCTGAAACTTACTCAAGAGCAAGCAGTAAGAGTTG
CTATGGGTATGGTTAAAAACTGGAAAGAATTAGGCTGTAAATCAGGTAAGTTAACTTGGGCACATCGTTCTGACGGATCA
GGTACAACTAAAGCCTTTACAAACTCTATGGAAGCGTTTTCAAAAACTTGGACACTAGGAACAGGTAAATCAGTTAAGTG
GCCGGCAGGTGTTGGTGCAAAAGGTAATTCTGGTGTAGCTGGAGTTATACAAAACACTCCTGGCGCAATCGGTTATGTTA
ACCAGTCTTATATAAAAGGTAATATTAAAGCTGCTGCTCTTCAGAATCTTTCTGGAGAATATGTAAAGCCTACAGTTGAG
GCAGGAGCAAAAGCTCTTAATGGCATTAATTTAGATGAAAATCTTGCGGGTAAAAATCCTAATCCAACTGCAAAAGGAGC
ATATCCTATAGCTTCATTAACATGGATACTTGCTTATGAAGAGGGTAATGGTAGAAACACTAAAGCTATCAAACAAGCCT
TTAATACATTGTTAAGTGATGAGTATCAAGATAAAGCTCCATCACTAGGATTTGTTCCTTTGAAAGGTGATATTCTTGAG
AAATCAAGAGCTGCTGTAAAAAGAATCGGTAAATAA

Upstream 100 bases:

>100_bases
AAGAGAAGGTTAAGAACTAATTAACCAAAGGATAAAATTTTAATTAATGAGTATTTGTGTATAAATTCGGTGCTATATAT
ATGTAGTCTTTAATTTAATC

Downstream 100 bases:

>100_bases
ACCGTAAGACAATATTTAAAAGGAGGAATTTATTTCTTCCTTTTTTTATGCAAACAAATATTTTGATAAACCTAAAATTA
AAGAAAAAAGAATAAGTATA

Product: periplasmic phosphate binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTSMNIAKKALVFTSAVAIAAGTSVPGTSVSAKTRLSGAGASFPAKIYTRWFKDLATSGGPRVNYQAVGSGSGRKAFIDQ
TVNFGASDDPMKDKDIAKVTRGLVQIPMVGGTIAFGYNYDCDLKLTQEQAVRVAMGMVKNWKELGCKSGKLTWAHRSDGS
GTTKAFTNSMEAFSKTWTLGTGKSVKWPAGVGAKGNSGVAGVIQNTPGAIGYVNQSYIKGNIKAAALQNLSGEYVKPTVE
AGAKALNGINLDENLAGKNPNPTAKGAYPIASLTWILAYEEGNGRNTKAIKQAFNTLLSDEYQDKAPSLGFVPLKGDILE
KSRAAVKRIGK

Sequences:

>Translated_331_residues
MTSMNIAKKALVFTSAVAIAAGTSVPGTSVSAKTRLSGAGASFPAKIYTRWFKDLATSGGPRVNYQAVGSGSGRKAFIDQ
TVNFGASDDPMKDKDIAKVTRGLVQIPMVGGTIAFGYNYDCDLKLTQEQAVRVAMGMVKNWKELGCKSGKLTWAHRSDGS
GTTKAFTNSMEAFSKTWTLGTGKSVKWPAGVGAKGNSGVAGVIQNTPGAIGYVNQSYIKGNIKAAALQNLSGEYVKPTVE
AGAKALNGINLDENLAGKNPNPTAKGAYPIASLTWILAYEEGNGRNTKAIKQAFNTLLSDEYQDKAPSLGFVPLKGDILE
KSRAAVKRIGK
>Mature_330_residues
TSMNIAKKALVFTSAVAIAAGTSVPGTSVSAKTRLSGAGASFPAKIYTRWFKDLATSGGPRVNYQAVGSGSGRKAFIDQT
VNFGASDDPMKDKDIAKVTRGLVQIPMVGGTIAFGYNYDCDLKLTQEQAVRVAMGMVKNWKELGCKSGKLTWAHRSDGSG
TTKAFTNSMEAFSKTWTLGTGKSVKWPAGVGAKGNSGVAGVIQNTPGAIGYVNQSYIKGNIKAAALQNLSGEYVKPTVEA
GAKALNGINLDENLAGKNPNPTAKGAYPIASLTWILAYEEGNGRNTKAIKQAFNTLLSDEYQDKAPSLGFVPLKGDILEK
SRAAVKRIGK

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=325, Percent_Identity=34.4615384615385, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 34800; Mature: 34669

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSMNIAKKALVFTSAVAIAAGTSVPGTSVSAKTRLSGAGASFPAKIYTRWFKDLATSGG
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEECCCCCCCCHHHHHHHHHHHHCCCC
PRVNYQAVGSGSGRKAFIDQTVNFGASDDPMKDKDIAKVTRGLVQIPMVGGTIAFGYNYD
CEEEEEEECCCCCCEEEEHHHHCCCCCCCCCCCHHHHHHHHHHEEECEECCEEEECCCCC
CDLKLTQEQAVRVAMGMVKNWKELGCKSGKLTWAHRSDGSGTTKAFTNSMEAFSKTWTLG
CCEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHEEECC
TGKSVKWPAGVGAKGNSGVAGVIQNTPGAIGYVNQSYIKGNIKAAALQNLSGEYVKPTVE
CCCCEECCCCCCCCCCCCEEEEEECCCCCEEECCHHHHCCCCHHHHHHCCCCCCCCCHHH
AGAKALNGINLDENLAGKNPNPTAKGAYPIASLTWILAYEEGNGRNTKAIKQAFNTLLSD
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCC
EYQDKAPSLGFVPLKGDILEKSRAAVKRIGK
HHHHCCCCCCEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSMNIAKKALVFTSAVAIAAGTSVPGTSVSAKTRLSGAGASFPAKIYTRWFKDLATSGG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEECCCCCCCCHHHHHHHHHHHHCCCC
PRVNYQAVGSGSGRKAFIDQTVNFGASDDPMKDKDIAKVTRGLVQIPMVGGTIAFGYNYD
CEEEEEEECCCCCCEEEEHHHHCCCCCCCCCCCHHHHHHHHHHEEECEECCEEEECCCCC
CDLKLTQEQAVRVAMGMVKNWKELGCKSGKLTWAHRSDGSGTTKAFTNSMEAFSKTWTLG
CCEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHEEECC
TGKSVKWPAGVGAKGNSGVAGVIQNTPGAIGYVNQSYIKGNIKAAALQNLSGEYVKPTVE
CCCCEECCCCCCCCCCCCEEEEEECCCCCEEECCHHHHCCCCHHHHHHCCCCCCCCCHHH
AGAKALNGINLDENLAGKNPNPTAKGAYPIASLTWILAYEEGNGRNTKAIKQAFNTLLSD
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCC
EYQDKAPSLGFVPLKGDILEKSRAAVKRIGK
HHHHCCCCCCEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6321434; 6365894; 7686882; 9278503; 9298646; 2215649; 9228942; 9671506 [H]