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The map label for this gene is sphS [H]

Identifier: 78779102

GI number: 78779102

Start: 667004

End: 668164

Strand: Direct

Name: sphS [H]

Synonym: PMT9312_0718

Alternate gene names: 78779102

Gene position: 667004-668164 (Clockwise)

Preceding gene: 78779101

Following gene: 78779106

Centisome position: 39.02

GC content: 26.87

Gene sequence:

>1161_bases
ATGAAAATGAAAACACTACAAGAAGTATTATTTTTTTTACATCAGAAGTGGAAAATAAAAGTCAAGCATTTTTCTCAATC
AAAAGAAAAAAAATTTGAAGTTGATAAAAATAAGTATAAAAAAACTATTGATTTCCCATTTGATAAAATTAAACCTCAAG
AAGTGTTGTCTTGGTTAGATTATTCATCGCAAGGGTGGATAATCTTATCATCTGATCTAACAATAAAATTTATCAATCAG
AAAGCTTTATCTCTTATTAGGGTTATCAAATATAAAGATGTTATTGGAAAAGCAATTAATGATATTAATGAGCTTGAAGT
ACTTAGTAATAAAATTCTATATTTAAGAAAAAAAGATTTCCCATTCAACCTTGATTTCATAATTGCGGGAGTACCCATTT
CGGTAAATATTGTTAGAGGAAGGAAAAAGAATTATTTAATATTATTGGAAAGTAAATTATCAATTGAATCGATAAAAAAA
CGACAAAATCAATTAATTAATGATGTGTCTCATGAACTGAAAACTCCTCTTACATCACTTATCTTAATTGGGGAAAGACT
TGAATCAGTAGTATCAAAAAAGGATAGGTATCTTGTTAAAAGACTTAAGAAAGAGTCAAAAAGATTAAGAAAAATGGCCG
AAGAGACTTTAGAGCTTTCTAAGCTAGAAAGTAACGAAGATTTTAATAAAAACAAAAAAATATCTATTTCAGATTTGATT
ATGGAATCTTGGCAAACACTAAAACCGCTTGCAGAAAAAAAGGATATAAAAATAAATTTATTTTCTCCAACAAAATATTA
TATATCTGGGGATATTGAAAATTTAAAAAGAGCATTTATAAATATTTTAGATAACGCTATTCGTTATTCCCCAACAAAAG
AGGGCATACAAATTGAAATTTTTAAGAGAGCTAGCTCTGTTGTTATAAGAGTTAGAGATAAAGGTATTGGATTAGAAGAA
AATGAATCAAATGACATTTTTTCTCGTTTTTATCGTGGGGATCCATCAAGGACTAAATTTAAGAAAAGTGGAAGTGGTTT
AGGTCTTTCAATTACAAAAAAAATAATTAATAACAATAAAGGTTTTATAAAGGCATTTAATCATAAAGATGGTGGAGCGA
TTATTGAAACTATCTTTCCGTGTCTTAATACAGATTTATAG

Upstream 100 bases:

>100_bases
CTGTAGATGTTCATGTTAGGTGGCTCAGAGAGAAATTAGAAGAAGATCCCTCAGCTCCAAAGTTTCTTAAAACTGTAAGA
GGTTTTGGATATAAGTTTGG

Downstream 100 bases:

>100_bases
GGAAAATTTAAAAAATATTAGGACATGATTTTTCTGCAATAAATTTTTTTAAATTCAAAACCCCTTCTCTTGTAAAAAGA
ATGCTTCCTTCTTTGGAGTC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MKMKTLQEVLFFLHQKWKIKVKHFSQSKEKKFEVDKNKYKKTIDFPFDKIKPQEVLSWLDYSSQGWIILSSDLTIKFINQ
KALSLIRVIKYKDVIGKAINDINELEVLSNKILYLRKKDFPFNLDFIIAGVPISVNIVRGRKKNYLILLESKLSIESIKK
RQNQLINDVSHELKTPLTSLILIGERLESVVSKKDRYLVKRLKKESKRLRKMAEETLELSKLESNEDFNKNKKISISDLI
MESWQTLKPLAEKKDIKINLFSPTKYYISGDIENLKRAFINILDNAIRYSPTKEGIQIEIFKRASSVVIRVRDKGIGLEE
NESNDIFSRFYRGDPSRTKFKKSGSGLGLSITKKIINNNKGFIKAFNHKDGGAIIETIFPCLNTDL

Sequences:

>Translated_386_residues
MKMKTLQEVLFFLHQKWKIKVKHFSQSKEKKFEVDKNKYKKTIDFPFDKIKPQEVLSWLDYSSQGWIILSSDLTIKFINQ
KALSLIRVIKYKDVIGKAINDINELEVLSNKILYLRKKDFPFNLDFIIAGVPISVNIVRGRKKNYLILLESKLSIESIKK
RQNQLINDVSHELKTPLTSLILIGERLESVVSKKDRYLVKRLKKESKRLRKMAEETLELSKLESNEDFNKNKKISISDLI
MESWQTLKPLAEKKDIKINLFSPTKYYISGDIENLKRAFINILDNAIRYSPTKEGIQIEIFKRASSVVIRVRDKGIGLEE
NESNDIFSRFYRGDPSRTKFKKSGSGLGLSITKKIINNNKGFIKAFNHKDGGAIIETIFPCLNTDL
>Mature_386_residues
MKMKTLQEVLFFLHQKWKIKVKHFSQSKEKKFEVDKNKYKKTIDFPFDKIKPQEVLSWLDYSSQGWIILSSDLTIKFINQ
KALSLIRVIKYKDVIGKAINDINELEVLSNKILYLRKKDFPFNLDFIIAGVPISVNIVRGRKKNYLILLESKLSIESIKK
RQNQLINDVSHELKTPLTSLILIGERLESVVSKKDRYLVKRLKKESKRLRKMAEETLELSKLESNEDFNKNKKISISDLI
MESWQTLKPLAEKKDIKINLFSPTKYYISGDIENLKRAFINILDNAIRYSPTKEGIQIEIFKRASSVVIRVRDKGIGLEE
NESNDIFSRFYRGDPSRTKFKKSGSGLGLSITKKIINNNKGFIKAFNHKDGGAIIETIFPCLNTDL

Specific function: Member of the two-component regulatory system sphR/sphS. Sensory kinase. Is involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to phos

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=227, Percent_Identity=31.7180616740088, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1790346, Length=223, Percent_Identity=32.7354260089686, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786912, Length=227, Percent_Identity=27.7533039647577, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788393, Length=233, Percent_Identity=27.0386266094421, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786600, Length=253, Percent_Identity=24.1106719367589, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788713, Length=228, Percent_Identity=25.8771929824561, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789808, Length=220, Percent_Identity=26.3636363636364, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1789149, Length=220, Percent_Identity=26.3636363636364, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789403, Length=220, Percent_Identity=28.1818181818182, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693157, Length=231, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787894, Length=232, Percent_Identity=23.7068965517241, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI48994928, Length=229, Percent_Identity=21.8340611353712, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788279, Length=208, Percent_Identity=28.8461538461538, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790551, Length=199, Percent_Identity=27.6381909547739, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788549, Length=226, Percent_Identity=26.5486725663717, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790436, Length=222, Percent_Identity=22.972972972973, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790861, Length=219, Percent_Identity=27.3972602739726, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44826; Mature: 44826

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS00018 EF_HAND_1 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKTLQEVLFFLHQKWKIKVKHFSQSKEKKFEVDKNKYKKTIDFPFDKIKPQEVLSWLD
CCHHHHHHHHHHHHCCCEEEEEHHCCCHHHHHCCCHHHHHEECCCCHHCCCHHHHHHHHC
YSSQGWIILSSDLTIKFINQKALSLIRVIKYKDVIGKAINDINELEVLSNKILYLRKKDF
CCCCCCEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
PFNLDFIIAGVPISVNIVRGRKKNYLILLESKLSIESIKKRQNQLINDVSHELKTPLTSL
CCCCEEEEECCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILIGERLESVVSKKDRYLVKRLKKESKRLRKMAEETLELSKLESNEDFNKNKKISISDLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHH
MESWQTLKPLAEKKDIKINLFSPTKYYISGDIENLKRAFINILDNAIRYSPTKEGIQIEI
HHHHHHHHHHHCCCCCEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
FKRASSVVIRVRDKGIGLEENESNDIFSRFYRGDPSRTKFKKSGSGLGLSITKKIINNNK
EECCCEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHCCCC
GFIKAFNHKDGGAIIETIFPCLNTDL
CEEEEECCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKMKTLQEVLFFLHQKWKIKVKHFSQSKEKKFEVDKNKYKKTIDFPFDKIKPQEVLSWLD
CCHHHHHHHHHHHHCCCEEEEEHHCCCHHHHHCCCHHHHHEECCCCHHCCCHHHHHHHHC
YSSQGWIILSSDLTIKFINQKALSLIRVIKYKDVIGKAINDINELEVLSNKILYLRKKDF
CCCCCCEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
PFNLDFIIAGVPISVNIVRGRKKNYLILLESKLSIESIKKRQNQLINDVSHELKTPLTSL
CCCCEEEEECCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILIGERLESVVSKKDRYLVKRLKKESKRLRKMAEETLELSKLESNEDFNKNKKISISDLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHH
MESWQTLKPLAEKKDIKINLFSPTKYYISGDIENLKRAFINILDNAIRYSPTKEGIQIEI
HHHHHHHHHHHCCCCCEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
FKRASSVVIRVRDKGIGLEENESNDIFSRFYRGDPSRTKFKKSGSGLGLSITKKIINNNK
EECCCEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHCCCC
GFIKAFNHKDGGAIIETIFPCLNTDL
CEEEEECCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497200 [H]