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The map label for this gene is mdeA [H]

Identifier: 78779027

GI number: 78779027

Start: 605612

End: 606940

Strand: Direct

Name: mdeA [H]

Synonym: PMT9312_0642

Alternate gene names: 78779027

Gene position: 605612-606940 (Clockwise)

Preceding gene: 78779026

Following gene: 78779028

Centisome position: 35.43

GC content: 38.9

Gene sequence:

>1329_bases
TTGAGCAACCAAAAGTTCGAGACTCTTCAGTTACATGCAGGTCAAGTGCCTGATCCAACCACAAATTCTAGAGCAGTACC
CATTTATCAAACTAGTTCCTATGTCTTTGATAATGCCGAGCATGGTGCGAATCTTTTTGGATTAAAAGAATTTGGAAATA
TTTACACTCGACTTATGAACCCCACCACAGATGTCTTCGAAAAAAGGATGGCTGCTTTGGAGGGAGGTATGGCAGCACTT
GCAACATCCTCAGGTCAGGCCGCTCAATTCTTGGCAATCGTGAACTGCATGACAGCAGGGGATAATTTTGTCTCTACTTC
TTTTCTATATGGTGGGACCTATAATCAATTTAAAGTACAATTTCCAAGATTAGGAATAGAAGTTAAATTTGCTGATGGCG
ATAGTATCGATAGTTTTAGAAATAAAATTGATGATAAAACCAAGGCAATATATGTCGAATCAATGGGAAATCCTAGATTC
AATATCCCAGATTTTGAGGGACTGTCTGCTTTGGCGAAGGAAAATGGAATTCCTTTAATAGTGGATAATACCCTTGGTGC
TGGTGGTGCTTTAATAAGACCAATTGATTTTGGAGCCGATGTTGTTGTGGAAAGTGCAACAAAATGGATCGGTGGACATG
GCACAAGTATCGGAGGGGTTATTGTTGATGCAGGAACCTTTGATTGGGGAAATGGTAAATTCCCACTAATGAGTGAGCCA
AGCGCTGCTTATCATGGGCTTGTTCATTGGGATGCTTTTGGTTTCGGTAGTGATATCTGCAAATCTTTGGGAGTACCTGA
TAATAGAAATATAGCTTTTGCGTTAAGAGCAAGACTTGAATGCCTGAGAGACTGGGGATCAGCTCAAAGTCCTTTTAATT
CGTTCTTGTTATTGCAGGGTTTGGAAACTCTAAGTTTAAGAATAGAAAGACAAACTTCTAATGCTCTTGAATTAGCAAAA
TGGTTGGATTCTAATTCTAATGTAAGTAGTGTTAATTACCCTGGCCTAGAATCTGATCCGTATTACTCAAGTGCCAAAAA
ATATACTACTGGAAGGGGAATGGGTTGCATGCTTATGTTTTCTCTTAATGGGGGTTATGAAAATGCAGTAAAATTTATTG
ATTCCTTAAAATTAGCGAGCCACCTTGCTAACGTGGGTGATTCAAAAACATTAGTAATTCATCCGGCTTCAACAACTCAT
CAGCAATTATCTGAAGAAGAACAATTATCTGCAGGTGTTACTCCTACGATGGTAAGAGTTTCTGTAGGAATTGAGCATAT
TGATGATATCAAAGCAGATTTCGAACAAGCACTTTCACAAATCACATAG

Upstream 100 bases:

>100_bases
ATTACCCTTCATTAAAAAAGGATTTATAAATATTTATCTTTTTTTTGTTTTTTTTTTGGACTTTTTTAAGTTAAATAGAT
AATAGGATTATTTAAAAATT

Downstream 100 bases:

>100_bases
GAAAGGAGATTTATTGGCTTTAATAATTCCTAGTAACTATCACAAGATTAGTGATGTTGAGAAAAATCATATATCTTGGA
TCGAACCAGAATTGGCAAAA

Product: putative O-Acetyl homoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSNQKFETLQLHAGQVPDPTTNSRAVPIYQTSSYVFDNAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRMAALEGGMAAL
ATSSGQAAQFLAIVNCMTAGDNFVSTSFLYGGTYNQFKVQFPRLGIEVKFADGDSIDSFRNKIDDKTKAIYVESMGNPRF
NIPDFEGLSALAKENGIPLIVDNTLGAGGALIRPIDFGADVVVESATKWIGGHGTSIGGVIVDAGTFDWGNGKFPLMSEP
SAAYHGLVHWDAFGFGSDICKSLGVPDNRNIAFALRARLECLRDWGSAQSPFNSFLLLQGLETLSLRIERQTSNALELAK
WLDSNSNVSSVNYPGLESDPYYSSAKKYTTGRGMGCMLMFSLNGGYENAVKFIDSLKLASHLANVGDSKTLVIHPASTTH
QQLSEEEQLSAGVTPTMVRVSVGIEHIDDIKADFEQALSQIT

Sequences:

>Translated_442_residues
MSNQKFETLQLHAGQVPDPTTNSRAVPIYQTSSYVFDNAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRMAALEGGMAAL
ATSSGQAAQFLAIVNCMTAGDNFVSTSFLYGGTYNQFKVQFPRLGIEVKFADGDSIDSFRNKIDDKTKAIYVESMGNPRF
NIPDFEGLSALAKENGIPLIVDNTLGAGGALIRPIDFGADVVVESATKWIGGHGTSIGGVIVDAGTFDWGNGKFPLMSEP
SAAYHGLVHWDAFGFGSDICKSLGVPDNRNIAFALRARLECLRDWGSAQSPFNSFLLLQGLETLSLRIERQTSNALELAK
WLDSNSNVSSVNYPGLESDPYYSSAKKYTTGRGMGCMLMFSLNGGYENAVKFIDSLKLASHLANVGDSKTLVIHPASTTH
QQLSEEEQLSAGVTPTMVRVSVGIEHIDDIKADFEQALSQIT
>Mature_441_residues
SNQKFETLQLHAGQVPDPTTNSRAVPIYQTSSYVFDNAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRMAALEGGMAALA
TSSGQAAQFLAIVNCMTAGDNFVSTSFLYGGTYNQFKVQFPRLGIEVKFADGDSIDSFRNKIDDKTKAIYVESMGNPRFN
IPDFEGLSALAKENGIPLIVDNTLGAGGALIRPIDFGADVVVESATKWIGGHGTSIGGVIVDAGTFDWGNGKFPLMSEPS
AAYHGLVHWDAFGFGSDICKSLGVPDNRNIAFALRARLECLRDWGSAQSPFNSFLLLQGLETLSLRIERQTSNALELAKW
LDSNSNVSSVNYPGLESDPYYSSAKKYTTGRGMGCMLMFSLNGGYENAVKFIDSLKLASHLANVGDSKTLVIHPASTTHQ
QLSEEEQLSAGVTPTMVRVSVGIEHIDDIKADFEQALSQIT

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=439, Percent_Identity=30.0683371298405, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI299473759, Length=439, Percent_Identity=27.1070615034169, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI25453487, Length=438, Percent_Identity=27.1689497716895, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1790375, Length=433, Percent_Identity=28.8683602771363, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1789383, Length=446, Percent_Identity=21.7488789237668, Blast_Score=102, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71980708, Length=455, Percent_Identity=29.6703296703297, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=438, Percent_Identity=32.1917808219178, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17560056, Length=437, Percent_Identity=30.6636155606407, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323333, Length=451, Percent_Identity=53.4368070953437, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6319307, Length=444, Percent_Identity=31.0810810810811, Blast_Score=176, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6321254, Length=463, Percent_Identity=25.2699784017279, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321170, Length=332, Percent_Identity=25, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19922554, Length=438, Percent_Identity=29.2237442922374, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47854; Mature: 47723

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQKFETLQLHAGQVPDPTTNSRAVPIYQTSSYVFDNAEHGANLFGLKEFGNIYTRLMN
CCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCEEECCCCCCCCEEEHHHHHHHHHHHCC
PTTDVFEKRMAALEGGMAALATSSGQAAQFLAIVNCMTAGDNFVSTSFLYGGTYNQFKVQ
CHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEE
FPRLGIEVKFADGDSIDSFRNKIDDKTKAIYVESMGNPRFNIPDFEGLSALAKENGIPLI
ECCCCEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEE
VDNTLGAGGALIRPIDFGADVVVESATKWIGGHGTSIGGVIVDAGTFDWGNGKFPLMSEP
EECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCEEECCCCCCCCCCCC
SAAYHGLVHWDAFGFGSDICKSLGVPDNRNIAFALRARLECLRDWGSAQSPFNSFLLLQG
CHHHCCEEEEECCCCHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHC
LETLSLRIERQTSNALELAKWLDSNSNVSSVNYPGLESDPYYSSAKKYTTGRGMGCMLMF
CCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
SLNGGYENAVKFIDSLKLASHLANVGDSKTLVIHPASTTHQQLSEEEQLSAGVTPTMVRV
EECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCHHHHHHCCCCCEEEEE
SVGIEHIDDIKADFEQALSQIT
EECHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNQKFETLQLHAGQVPDPTTNSRAVPIYQTSSYVFDNAEHGANLFGLKEFGNIYTRLMN
CCCCEEEEEEECCCCCCCCCCCEEEEEEECCCEEECCCCCCCCEEEHHHHHHHHHHHCC
PTTDVFEKRMAALEGGMAALATSSGQAAQFLAIVNCMTAGDNFVSTSFLYGGTYNQFKVQ
CHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEE
FPRLGIEVKFADGDSIDSFRNKIDDKTKAIYVESMGNPRFNIPDFEGLSALAKENGIPLI
ECCCCEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEE
VDNTLGAGGALIRPIDFGADVVVESATKWIGGHGTSIGGVIVDAGTFDWGNGKFPLMSEP
EECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCEEECCCCCCCCCCCC
SAAYHGLVHWDAFGFGSDICKSLGVPDNRNIAFALRARLECLRDWGSAQSPFNSFLLLQG
CHHHCCEEEEECCCCHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHC
LETLSLRIERQTSNALELAKWLDSNSNVSSVNYPGLESDPYYSSAKKYTTGRGMGCMLMF
CCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
SLNGGYENAVKFIDSLKLASHLANVGDSKTLVIHPASTTHQQLSEEEQLSAGVTPTMVRV
EECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCHHHHHHCCCCCEEEEE
SVGIEHIDDIKADFEQALSQIT
EECHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]