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The map label for this gene is lytC [H]

Identifier: 78779001

GI number: 78779001

Start: 581443

End: 582528

Strand: Direct

Name: lytC [H]

Synonym: PMT9312_0616

Alternate gene names: 78779001

Gene position: 581443-582528 (Clockwise)

Preceding gene: 78779000

Following gene: 78779002

Centisome position: 34.02

GC content: 32.32

Gene sequence:

>1086_bases
ATGATAAAGTTTTTAGATAATAAACTTTTTCGTTATATATCCGTTTTTTTATTTTTAAATTCTGCAATCCTCCCTTTGAA
ATCTTCAAGTGCTCTGGCAGCATGGGCGATCAAAACTAATGGTGTTTTAGAATTAAGAACTAAATCAAATACAAATTTAA
AAGCATACTTTCAGAAGTCGAACCAAATATATGGCGATAGATTCTGGGTTGATTTCCCCGGAGAATTAAAAAATCCCAGA
ATAATAAAAGGTAATGGTCCAATAAAAGAAATTAGATTAGGTAAACCAAATAATGGTAAAACAAGACTAGTAATTGAATT
TAAGGAAGAAACTTATTTGAAACCTTTGACTTGGCGAATGGTTGGATTAGATCAAAATAGGTGGTCAATTAAGCTATTTA
ACCCAAAAAATTCATTTAAAAAGATTGGTGAAGGTTTAGTTATTAAGAGAACAGGAAATATTCAAGCAAATCAAAACTCT
ATTTATAATAAGAAAAGTAAGTATGATTACTTTAAATTACCAAAAGTAAAACAAAATAAATTTTCGGTTGTTATCGATCC
AGGGCATGGAGGGCCTGATCCAGGGGCAATTGGTATTGGCGGTATTAGGGAAACAGATGTTGTACTAGAGGTCTCCAAAA
TAGTTGAAAATTTACTTTCTGAGAAAGGTGTCAAAGTAGTTTTAACTAGAAAAAATGAAGTTGATTTGGGTTTATCTCCA
AGAGTTTCCTTCGCTAACAATGCTGATGCAGATATCTTTGTAAGTATTCATGCAAATGCCTCAAGAGGTAAAAGAAAGGA
TATTAATGGGTTGGAAACCTTTTACTTTAGAGGTTGGAGAGGTAGATTACTCGCCAGAAAGATTCAAAAACAAATTCTAA
GAGTTTCCCCTGGGAGTCCTGATCGAGGAGTTAAACAAGGTAGATTTTACGTAATTAAAAATACTAGAATGCCTGCAGTT
CTTGTAGAAATTGGGTTTTTAACAGGAAGATTAGATGCAAGAAGATTAGAAAAAAAAACACATCGTAAAAGATTAGCTTA
TGCAATTGCAAAAGGCATCCTTGAATATCTAGATAAAGTAGGGTGA

Upstream 100 bases:

>100_bases
AGGGGGCCGCAATAGCGCCTGCGGATAAAAAAAGAGTAAAGAAGATTAGGGAGCAGATGCCAAGCCTTAAACATAGAAAA
AATAAATTGTTTTCAAACTA

Downstream 100 bases:

>100_bases
AACTTAAAATAGGTATATTTGATAGTGGAATAGGTGGATTTACTATTCTCAATTCTTTACTAAAAACACGTAATGATGTT
GAAGTTTTTTATTTGGCGGA

Product: cell wall hydrolase/autolysin

Products: NA

Alternate protein names: Cell wall hydrolase; Cell wall-associated polypeptide CWBP49; CWBP49; Major autolysin [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MIKFLDNKLFRYISVFLFLNSAILPLKSSSALAAWAIKTNGVLELRTKSNTNLKAYFQKSNQIYGDRFWVDFPGELKNPR
IIKGNGPIKEIRLGKPNNGKTRLVIEFKEETYLKPLTWRMVGLDQNRWSIKLFNPKNSFKKIGEGLVIKRTGNIQANQNS
IYNKKSKYDYFKLPKVKQNKFSVVIDPGHGGPDPGAIGIGGIRETDVVLEVSKIVENLLSEKGVKVVLTRKNEVDLGLSP
RVSFANNADADIFVSIHANASRGKRKDINGLETFYFRGWRGRLLARKIQKQILRVSPGSPDRGVKQGRFYVIKNTRMPAV
LVEIGFLTGRLDARRLEKKTHRKRLAYAIAKGILEYLDKVG

Sequences:

>Translated_361_residues
MIKFLDNKLFRYISVFLFLNSAILPLKSSSALAAWAIKTNGVLELRTKSNTNLKAYFQKSNQIYGDRFWVDFPGELKNPR
IIKGNGPIKEIRLGKPNNGKTRLVIEFKEETYLKPLTWRMVGLDQNRWSIKLFNPKNSFKKIGEGLVIKRTGNIQANQNS
IYNKKSKYDYFKLPKVKQNKFSVVIDPGHGGPDPGAIGIGGIRETDVVLEVSKIVENLLSEKGVKVVLTRKNEVDLGLSP
RVSFANNADADIFVSIHANASRGKRKDINGLETFYFRGWRGRLLARKIQKQILRVSPGSPDRGVKQGRFYVIKNTRMPAV
LVEIGFLTGRLDARRLEKKTHRKRLAYAIAKGILEYLDKVG
>Mature_361_residues
MIKFLDNKLFRYISVFLFLNSAILPLKSSSALAAWAIKTNGVLELRTKSNTNLKAYFQKSNQIYGDRFWVDFPGELKNPR
IIKGNGPIKEIRLGKPNNGKTRLVIEFKEETYLKPLTWRMVGLDQNRWSIKLFNPKNSFKKIGEGLVIKRTGNIQANQNS
IYNKKSKYDYFKLPKVKQNKFSVVIDPGHGGPDPGAIGIGGIRETDVVLEVSKIVENLLSEKGVKVVLTRKNEVDLGLSP
RVSFANNADADIFVSIHANASRGKRKDINGLETFYFRGWRGRLLARKIQKQILRVSPGSPDRGVKQGRFYVIKNTRMPAV
LVEIGFLTGRLDARRLEKKTHRKRLAYAIAKGILEYLDKVG

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. Has a high affinity for teich

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=220, Percent_Identity=28.1818181818182, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788776, Length=88, Percent_Identity=45.4545454545455, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790611, Length=227, Percent_Identity=26.8722466960352, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007253
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF04122 CW_binding_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 40934; Mature: 40934

Theoretical pI: Translated: 11.01; Mature: 11.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKFLDNKLFRYISVFLFLNSAILPLKSSSALAAWAIKTNGVLELRTKSNTNLKAYFQKS
CCEEHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCEEEEEECCCCCEEEEEECC
NQIYGDRFWVDFPGELKNPRIIKGNGPIKEIRLGKPNNGKTRLVIEFKEETYLKPLTWRM
CCEECCEEEEECCCCCCCCEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCCCCEEEEE
VGLDQNRWSIKLFNPKNSFKKIGEGLVIKRTGNIQANQNSIYNKKSKYDYFKLPKVKQNK
EEECCCCEEEEEECCCHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCCEEECCCCCCCC
FSVVIDPGHGGPDPGAIGIGGIRETDVVLEVSKIVENLLSEKGVKVVLTRKNEVDLGLSP
EEEEEECCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
RVSFANNADADIFVSIHANASRGKRKDINGLETFYFRGWRGRLLARKIQKQILRVSPGSP
CEECCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHEECCCCCC
DRGVKQGRFYVIKNTRMPAVLVEIGFLTGRLDARRLEKKTHRKRLAYAIAKGILEYLDKV
CCCCCCCEEEEEECCCCCEEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
G
C
>Mature Secondary Structure
MIKFLDNKLFRYISVFLFLNSAILPLKSSSALAAWAIKTNGVLELRTKSNTNLKAYFQKS
CCEEHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCEEEEEECCCCCEEEEEECC
NQIYGDRFWVDFPGELKNPRIIKGNGPIKEIRLGKPNNGKTRLVIEFKEETYLKPLTWRM
CCEECCEEEEECCCCCCCCEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCCCCEEEEE
VGLDQNRWSIKLFNPKNSFKKIGEGLVIKRTGNIQANQNSIYNKKSKYDYFKLPKVKQNK
EEECCCCEEEEEECCCHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCCEEECCCCCCCC
FSVVIDPGHGGPDPGAIGIGGIRETDVVLEVSKIVENLLSEKGVKVVLTRKNEVDLGLSP
EEEEEECCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
RVSFANNADADIFVSIHANASRGKRKDINGLETFYFRGWRGRLLARKIQKQILRVSPGSP
CEECCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHEECCCCCC
DRGVKQGRFYVIKNTRMPAVLVEIGFLTGRLDARRLEKKTHRKRLAYAIAKGILEYLDKV
CCCCCCCEEEEEECCCCCEEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1357079; 1682302; 9384377 [H]