Click here to switch to the map view.

The map label for this gene is yafV [C]

Identifier: 78779000

GI number: 78779000

Start: 580616

End: 581443

Strand: Direct

Name: yafV [C]

Synonym: PMT9312_0615

Alternate gene names: 78779000

Gene position: 580616-581443 (Clockwise)

Preceding gene: 78778999

Following gene: 78779001

Centisome position: 33.97

GC content: 38.89

Gene sequence:

>828_bases
TTGACTGATTTTTTGGTGGCTGCATTGCAAATTACAAGCACTTCTAATGTAGAAGCAAATTTTGTTGAAGCGGAGGAACA
GATTGAATTAGCAGCTCGAAGAGGTGCTGAGTTAATCGGATTGCCTGAGAATTTTGCTTTTTTAGGAGAAGATGACGAAA
AACTTAGATTAGCTCCTGAATTGTCAATGAAGTGTACAAACTTCCTAAAAACTATGTCACAGAGATATCAAGTTTTTCTA
TTGGGAGGAGGATATCCTGTTCCAGCTGGTGATGATAGACATACTTTAAATAGATCAGCACTCTTTGGAAGAGATGGACA
GGTTTTGGCAAAATATGACAAAATCCATTTGTTCGATGTTGATTTGCCAGACGGAAATTTATATAAGGAATCATCTACTA
TTTTATCTGGGGAGGAGTATCCACCTGTTGTAGATGTCCCAGGTTTATGCAAAATAGGGTTATCGATTTGTTACGACGTT
AGATTCCCTGAACTTTATAGATATTTGTCTTCTAATGGTGCAGAGCTAATTATGATTCCCGCAGCTTTTACAGCATTTAC
TGGAAAAGATCATTGGCAAATCCTATTACAAGCAAGAGCGATTGAGAATACAGCATATGTAGTTGCTCCAGCGCAAACTG
GGGTTCATTATGGAAGAAGGCAAAGTCATGGCCATGCAATGGTAATTGACCCATGGGGCACTGTTTTGTCTGATGCTGGG
AAAACTCAGGGGGCCGCAATAGCGCCTGCGGATAAAAAAAGAGTAAAGAAGATTAGGGAGCAGATGCCAAGCCTTAAACA
TAGAAAAAATAAATTGTTTTCAAACTAA

Upstream 100 bases:

>100_bases
TTGCTCAGAACTTGATTGCTTAGATATTGTTCCTGCTCAAGTTGAAAGAGGTGTGATTCGTGCCTCATGATTTACGAATT
GGTTTATTAGGAGTACTGTT

Downstream 100 bases:

>100_bases
TGATAAAGTTTTTAGATAATAAACTTTTTCGTTATATATCCGTTTTTTTATTTTTAAATTCTGCAATCCTCCCTTTGAAA
TCTTCAAGTGCTCTGGCAGC

Product: putative nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTDFLVAALQITSTSNVEANFVEAEEQIELAARRGAELIGLPENFAFLGEDDEKLRLAPELSMKCTNFLKTMSQRYQVFL
LGGGYPVPAGDDRHTLNRSALFGRDGQVLAKYDKIHLFDVDLPDGNLYKESSTILSGEEYPPVVDVPGLCKIGLSICYDV
RFPELYRYLSSNGAELIMIPAAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGVHYGRRQSHGHAMVIDPWGTVLSDAG
KTQGAAIAPADKKRVKKIREQMPSLKHRKNKLFSN

Sequences:

>Translated_275_residues
MTDFLVAALQITSTSNVEANFVEAEEQIELAARRGAELIGLPENFAFLGEDDEKLRLAPELSMKCTNFLKTMSQRYQVFL
LGGGYPVPAGDDRHTLNRSALFGRDGQVLAKYDKIHLFDVDLPDGNLYKESSTILSGEEYPPVVDVPGLCKIGLSICYDV
RFPELYRYLSSNGAELIMIPAAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGVHYGRRQSHGHAMVIDPWGTVLSDAG
KTQGAAIAPADKKRVKKIREQMPSLKHRKNKLFSN
>Mature_274_residues
TDFLVAALQITSTSNVEANFVEAEEQIELAARRGAELIGLPENFAFLGEDDEKLRLAPELSMKCTNFLKTMSQRYQVFLL
GGGYPVPAGDDRHTLNRSALFGRDGQVLAKYDKIHLFDVDLPDGNLYKESSTILSGEEYPPVVDVPGLCKIGLSICYDVR
FPELYRYLSSNGAELIMIPAAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGVHYGRRQSHGHAMVIDPWGTVLSDAGK
TQGAAIAPADKKRVKKIREQMPSLKHRKNKLFSN

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=275, Percent_Identity=39.2727272727273, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI297632350, Length=275, Percent_Identity=39.2727272727273, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI5031947, Length=275, Percent_Identity=39.2727272727273, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI9910460, Length=274, Percent_Identity=33.5766423357664, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI297632346, Length=193, Percent_Identity=37.3056994818653, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17556280, Length=272, Percent_Identity=39.7058823529412, Blast_Score=207, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6322335, Length=303, Percent_Identity=33.003300330033, Blast_Score=166, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6323383, Length=282, Percent_Identity=31.5602836879433, Blast_Score=146, Evalue=4e-36,
Organism=Drosophila melanogaster, GI17933642, Length=271, Percent_Identity=35.4243542435424, Blast_Score=164, Evalue=4e-41,
Organism=Drosophila melanogaster, GI21355835, Length=276, Percent_Identity=31.5217391304348, Blast_Score=147, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 30474; Mature: 30343

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFLVAALQITSTSNVEANFVEAEEQIELAARRGAELIGLPENFAFLGEDDEKLRLAPE
CCCCEEEEEEEECCCCCEEEEECHHHHHHHHHHCCCEEEECCCCCEEECCCCCEEEECCC
LSMKCTNFLKTMSQRYQVFLLGGGYPVPAGDDRHTLNRSALFGRDGQVLAKYDKIHLFDV
HHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCHHEECCCCCEEEEECEEEEEEE
DLPDGNLYKESSTILSGEEYPPVVDVPGLCKIGLSICYDVRFPELYRYLSSNGAELIMIP
ECCCCCCEECCCCEECCCCCCCEECCCCHHHHHHHHEEECCCHHHHHHHHCCCCEEEEEE
AAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGVHYGRRQSHGHAMVIDPWGTVLSDAG
CEEEECCCCCHHEEEEEHEEECCEEEEEEECHHCCCCCCCCCCCCEEEECCCHHHHHCCC
KTQGAAIAPADKKRVKKIREQMPSLKHRKNKLFSN
CCCCCEECCCHHHHHHHHHHHCCHHHHHHHHCCCC
>Mature Secondary Structure 
TDFLVAALQITSTSNVEANFVEAEEQIELAARRGAELIGLPENFAFLGEDDEKLRLAPE
CCCEEEEEEEECCCCCEEEEECHHHHHHHHHHCCCEEEECCCCCEEECCCCCEEEECCC
LSMKCTNFLKTMSQRYQVFLLGGGYPVPAGDDRHTLNRSALFGRDGQVLAKYDKIHLFDV
HHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCHHEECCCCCEEEEECEEEEEEE
DLPDGNLYKESSTILSGEEYPPVVDVPGLCKIGLSICYDVRFPELYRYLSSNGAELIMIP
ECCCCCCEECCCCEECCCCCCCEECCCCHHHHHHHHEEECCCHHHHHHHHCCCCEEEEEE
AAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGVHYGRRQSHGHAMVIDPWGTVLSDAG
CEEEECCCCCHHEEEEEHEEECCEEEEEEECHHCCCCCCCCCCCCEEEECCCHHHHHCCC
KTQGAAIAPADKKRVKKIREQMPSLKHRKNKLFSN
CCCCCEECCCHHHHHHHHHHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]