Click here to switch to the map view.

The map label for this gene is purA [H]

Identifier: 78778892

GI number: 78778892

Start: 473988

End: 475298

Strand: Reverse

Name: purA [H]

Synonym: PMT9312_0507

Alternate gene names: 78778892

Gene position: 475298-473988 (Counterclockwise)

Preceding gene: 78778893

Following gene: 78778891

Centisome position: 27.81

GC content: 36.77

Gene sequence:

>1311_bases
TTGGCTAATGTTGTTGTAATCGGGGCCCAATGGGGTGACGAAGGAAAAGGTAAAATAACAGATTTACTTAGTCGTTCGGC
TGATGTTGTCGTTCGTTACCAAGGAGGTGTAAATGCTGGTCACACTATAGTTGTAGATGATAAAGTCTTAAAATTACATT
TAATTCCTTCAGGGATACTCTATAAAAATAAAACTTGTTTAATTGGATCAGGAACAGTTGTAGATCCGAAAATCTTGCTG
AAAGAAATTGATATGTTAATTGATAATGAAATTGACATCTCAGGATTAAAACTTTCATCAACATCACATGTAACAATGCC
TTACCACCGAATATTAGATGAAGCTATGGAAGCTGATAGAGGCTCAAATAAAATAGGGACGACAGGTCGTGGAATTGGCC
CTACATATGCTGATAAGTCGCAAAGGAATGGTATTAGAGTAAGAGATTTACTTAATAAGGAAAGATTAAGAGATGTTATC
GAAATTCCATTAAGAGAAAAAAACGGTCTATTAGAAAAAATCTATGGTATTAAACCACTCAAAACAGAAGACATTGTTGA
AGAATATCTTGACTATGGTGAAAGATTATCAAAACATGTAGTCGACTGTACTAGAACTATACATTCAGCCTCAAAAAATA
AGAAGAATATTCTATTTGAAGGTGCTCAGGGGACTCTGCTTGACTTAGACCATGGGACATATCCTTTTGTTACCTCATCA
AACCCTATATCAGGAGGTGCTTGCATTGGAGCTGGAGTTGGCCCTACGTTAATCGATAGAGTTATAGGAGTCGCAAAAGC
ATACACCACAAGAGTAGGTGAGGGCCCATTCCCAACGGAATTACAGGGAAGTATCAATGATCAACTCTGTGATAGAGGTA
GTGAATTTGGAACCACTACTGGTAGAAGGAGGAGGTGTGGGTGGTTTGATGGAGTTATTGGTAAATATGCTGTATCTGTA
AATGGTCTTGATTGTTTAGCAGTTACGAAACTAGATGTACTAGATGAATTAGATGAGATTCAAGTTTGCATTGCATATGA
TCTAGATGGAGAGCGAATAGACTACTTCCCTACAAATTCAGATGACTTAAAAAAATGCAAGCCAATCTTCAAGAAATTAA
AAGGTTGGCAATGTTCAACTGCAAATTGTAGAAATCTATCTGATCTCCCTCAAAATGCCATGAATTACCTCAGATTTTTA
GCTGAATTAATGGAAGTTCCAATTGCTATTGTCTCATTGGGGGCGAATAGAGATCAAACTATAGTAATTGAAGACCCAAT
ACATGGACCTAAAAGAGCTCTTCTAAGATAA

Upstream 100 bases:

>100_bases
GGTTCTAAGAGAAAGTTGATACAATTCATTTACTTTTTAAAAGTAAACCAAGATTTTTGAATATATTAAATATTCGCACA
AAAATAATGCTCTTCTAAAA

Downstream 100 bases:

>100_bases
TTTACTAAAAAATTAATGAGGACATCTTTTAGACATTTTGAACAAAATAAAATAGTTGACCTTGTTGGTTTGGGCAACGC
AATAGTAGATATTATTGTCA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGILYKNKTCLIGSGTVVDPKILL
KEIDMLIDNEIDISGLKLSSTSHVTMPYHRILDEAMEADRGSNKIGTTGRGIGPTYADKSQRNGIRVRDLLNKERLRDVI
EIPLREKNGLLEKIYGIKPLKTEDIVEEYLDYGERLSKHVVDCTRTIHSASKNKKNILFEGAQGTLLDLDHGTYPFVTSS
NPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELQGSINDQLCDRGSEFGTTTGRRRRCGWFDGVIGKYAVSV
NGLDCLAVTKLDVLDELDEIQVCIAYDLDGERIDYFPTNSDDLKKCKPIFKKLKGWQCSTANCRNLSDLPQNAMNYLRFL
AELMEVPIAIVSLGANRDQTIVIEDPIHGPKRALLR

Sequences:

>Translated_436_residues
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGILYKNKTCLIGSGTVVDPKILL
KEIDMLIDNEIDISGLKLSSTSHVTMPYHRILDEAMEADRGSNKIGTTGRGIGPTYADKSQRNGIRVRDLLNKERLRDVI
EIPLREKNGLLEKIYGIKPLKTEDIVEEYLDYGERLSKHVVDCTRTIHSASKNKKNILFEGAQGTLLDLDHGTYPFVTSS
NPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELQGSINDQLCDRGSEFGTTTGRRRRCGWFDGVIGKYAVSV
NGLDCLAVTKLDVLDELDEIQVCIAYDLDGERIDYFPTNSDDLKKCKPIFKKLKGWQCSTANCRNLSDLPQNAMNYLRFL
AELMEVPIAIVSLGANRDQTIVIEDPIHGPKRALLR
>Mature_435_residues
ANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGILYKNKTCLIGSGTVVDPKILLK
EIDMLIDNEIDISGLKLSSTSHVTMPYHRILDEAMEADRGSNKIGTTGRGIGPTYADKSQRNGIRVRDLLNKERLRDVIE
IPLREKNGLLEKIYGIKPLKTEDIVEEYLDYGERLSKHVVDCTRTIHSASKNKKNILFEGAQGTLLDLDHGTYPFVTSSN
PISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELQGSINDQLCDRGSEFGTTTGRRRRCGWFDGVIGKYAVSVN
GLDCLAVTKLDVLDELDEIQVCIAYDLDGERIDYFPTNSDDLKKCKPIFKKLKGWQCSTANCRNLSDLPQNAMNYLRFLA
ELMEVPIAIVSLGANRDQTIVIEDPIHGPKRALLR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=425, Percent_Identity=42.3529411764706, Blast_Score=333, Evalue=1e-91,
Organism=Homo sapiens, GI22748717, Length=431, Percent_Identity=44.0835266821346, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI40316944, Length=402, Percent_Identity=42.2885572139304, Blast_Score=285, Evalue=4e-77,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=47.3067915690867, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=39.5348837209302, Blast_Score=305, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=39.5348837209302, Blast_Score=305, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6324109, Length=436, Percent_Identity=42.6605504587156, Blast_Score=326, Evalue=5e-90,
Organism=Drosophila melanogaster, GI21356033, Length=430, Percent_Identity=42.093023255814, Blast_Score=337, Evalue=7e-93,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47896; Mature: 47764

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGIL
CCCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCCEE
YKNKTCLIGSGTVVDPKILLKEIDMLIDNEIDISGLKLSSTSHVTMPYHRILDEAMEADR
ECCCEEEEECCCEECHHHHHHHHHHHHCCCCCCCCEEECCCCCEECHHHHHHHHHHHHCC
GSNKIGTTGRGIGPTYADKSQRNGIRVRDLLNKERLRDVIEIPLREKNGLLEKIYGIKPL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHCCCCC
KTEDIVEEYLDYGERLSKHVVDCTRTIHSASKNKKNILFEGAQGTLLDLDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCEEECC
NPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELQGSINDQLCDRGSEFGTTT
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCC
GRRRRCGWFDGVIGKYAVSVNGLDCLAVTKLDVLDELDEIQVCIAYDLDGERIDYFPTNS
CCCCCCCHHHHHHHHEEEEECCCEEEEEHHHHHHHHHCCEEEEEEEECCCCEEEECCCCC
DDLKKCKPIFKKLKGWQCSTANCRNLSDLPQNAMNYLRFLAELMEVPIAIVSLGANRDQT
HHHHHHHHHHHHHCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCE
IVIEDPIHGPKRALLR
EEEECCCCCCHHHHCC
>Mature Secondary Structure 
ANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGIL
CCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCCEE
YKNKTCLIGSGTVVDPKILLKEIDMLIDNEIDISGLKLSSTSHVTMPYHRILDEAMEADR
ECCCEEEEECCCEECHHHHHHHHHHHHCCCCCCCCEEECCCCCEECHHHHHHHHHHHHCC
GSNKIGTTGRGIGPTYADKSQRNGIRVRDLLNKERLRDVIEIPLREKNGLLEKIYGIKPL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHCCCCC
KTEDIVEEYLDYGERLSKHVVDCTRTIHSASKNKKNILFEGAQGTLLDLDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCEEECC
NPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELQGSINDQLCDRGSEFGTTT
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCC
GRRRRCGWFDGVIGKYAVSVNGLDCLAVTKLDVLDELDEIQVCIAYDLDGERIDYFPTNS
CCCCCCCHHHHHHHHEEEEECCCEEEEEHHHHHHHHHCCEEEEEEEECCCCEEEECCCCC
DDLKKCKPIFKKLKGWQCSTANCRNLSDLPQNAMNYLRFLAELMEVPIAIVSLGANRDQT
HHHHHHHHHHHHHCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCE
IVIEDPIHGPKRALLR
EEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA