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The map label for this gene is mrcB [H]

Identifier: 78778811

GI number: 78778811

Start: 402100

End: 403869

Strand: Reverse

Name: mrcB [H]

Synonym: PMT9312_0426

Alternate gene names: 78778811

Gene position: 403869-402100 (Counterclockwise)

Preceding gene: 78778812

Following gene: 78778803

Centisome position: 23.63

GC content: 27.18

Gene sequence:

>1770_bases
GTGCAAAAGATTAAATTCAAATCTTTTGTTTTAATAATTCCAATATTAATTTTTTCTGGAATATCTTTTTATTTTTTTAG
TCTAATATCTAGTACGTTAAAATTTGACAACTCTAAAAATCAAAGATCTAACGTAACCAAATATTCTTATGTAATATCAT
CTTCTGATAATAAGATACTAAGCAAATTAAGCCGCAAATTTGAAATAGATAATAGTTATCATAAAATTCCAATTTTCCTC
AAACATTCTTTTATATCTTCTGAGGATAAAAGATTTTATATCCATAATGGAATAGATTTAAAAAGTATTTCACGTGCCCT
TATTCAAAATATTAGAAGTGGTTATGTTAAAGAGGGAGGAAGTACGATTACACAACAAGTTGCTAGATTACTTTTTCTAA
ATAATGATTTAAGTTTTCAAAGAAAACTCAAAGAAATATTTATATCACTCATACTTGAATTTAGATATAACAAAAATCAA
ATTTTAAAATTATATTTAAATAATATTTATCTAGGATCAGGAGCATACGGGGTAGACGAGGCTGCCAAAGTTTATTTTGG
AAAATTCATAGAAGAATTGACATTATCAGAGATCGCATTAATTACAGGATTAGCTCCAGCTCCATCAATTTATTCACCTT
ATCAAAATTTAGAACTAGCTATTAAAAATAGAAATAAAGTTCTTGAATCAATGTATGTTGATGGATATATTTCTCTTGCA
AATAAGAATAAGGCTATTAAAGAAAAAATCAAATTAAATTATCAAACAGCTGATAATTTTTTAGATGATAAATTATTAAT
AAATTTTATTCTTGAGGAAACAGATAAAAAAATTGGAAGTAAAAATGATTATGAGTTTTTAAGAATTAAATCTTCTATCA
ACAAAGATTGGCAAGAAAAAGGTCAAAAAATATCAAGATATGCAGGGCCTAAAGAACTTGAATTTGCTCTGTTATCTATC
GAATCAAACACAGGACTAATCAGGACAATGATAACCAGCAAAAATCCATCAATTAATGAATACAATAGAGTGATTTCATC
TGTAAGACCTTTAGGTTCTACATTTAAAATAATCCCTTATGCTGCTGCATTAATAGAAGGAATAAAATTAAGCGATAAAT
TTGAAGACTTACCAATATGCTGGGAAAGTTATTGCCCAAAAAATTTTTCAGAAGACTATAGAGGTTCAATATCTTTGATT
GAATCATTTAAAAGTTCATCAAATATCGTTCCAATATCAATAACAAAAAAAATCGGCTTAAAAAATATTATTAATTTAGC
AAATTCATTTGGACTAGGTTACGAGCAAGAGTTTGAGGAATTCCCATCGTTAGCTATTGGCTCCTACGGAGATAATCTTT
TAAACATCACAAATGCATATTCTGCAATTAACAATAATGGGAAGATTCAAAGCCCTGAAATCATAGAAAAAATAGAATCA
TTTAAAAAACAACCTATTTGGGAAAACAAATCTATTTCCACGAAAATATTAGATTTAAAAATAAACAAGAAAATAAATAA
ACTTCTTGAAAAATCTGTAAAAGAAGGGACTTCAAAAGCAGCCTCTATAAAAGGAAAGAAAATTTATGGCAAAACAGGAA
CATCTGATGGAAATAAAGATCTCTGGTTTATTGGTTCCATTAATAACCTTACAACAGGGATCTGGATAGGTTACGACGAT
AACAAGGAATCTGAATTATCTAGTGGGAATGCGGCTTATTTATGGAAGAAGTTCATATCTGAAATTTATAAAATTCCAAT
TAAAAAATAA

Upstream 100 bases:

>100_bases
AAGTTTGATGTCAAATATCAAGCAAGTGCCCAACCGTTCCTTATAACTGGAATGTTAGTTACAGCTTTAGCGATAGGACA
TAGTTTTTTAGTTGCTTAAG

Downstream 100 bases:

>100_bases
ATTATATTTTTAAGATTTATAAGAAATTATTGCAGAATAAAATCCATCACCAGGATAATCCAAGCTAGGTAAAATTTGCT
TTTGGCCAACCAATTTTAAA

Product: putative penicillin binding protein

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MQKIKFKSFVLIIPILIFSGISFYFFSLISSTLKFDNSKNQRSNVTKYSYVISSSDNKILSKLSRKFEIDNSYHKIPIFL
KHSFISSEDKRFYIHNGIDLKSISRALIQNIRSGYVKEGGSTITQQVARLLFLNNDLSFQRKLKEIFISLILEFRYNKNQ
ILKLYLNNIYLGSGAYGVDEAAKVYFGKFIEELTLSEIALITGLAPAPSIYSPYQNLELAIKNRNKVLESMYVDGYISLA
NKNKAIKEKIKLNYQTADNFLDDKLLINFILEETDKKIGSKNDYEFLRIKSSINKDWQEKGQKISRYAGPKELEFALLSI
ESNTGLIRTMITSKNPSINEYNRVISSVRPLGSTFKIIPYAAALIEGIKLSDKFEDLPICWESYCPKNFSEDYRGSISLI
ESFKSSSNIVPISITKKIGLKNIINLANSFGLGYEQEFEEFPSLAIGSYGDNLLNITNAYSAINNNGKIQSPEIIEKIES
FKKQPIWENKSISTKILDLKINKKINKLLEKSVKEGTSKAASIKGKKIYGKTGTSDGNKDLWFIGSINNLTTGIWIGYDD
NKESELSSGNAAYLWKKFISEIYKIPIKK

Sequences:

>Translated_589_residues
MQKIKFKSFVLIIPILIFSGISFYFFSLISSTLKFDNSKNQRSNVTKYSYVISSSDNKILSKLSRKFEIDNSYHKIPIFL
KHSFISSEDKRFYIHNGIDLKSISRALIQNIRSGYVKEGGSTITQQVARLLFLNNDLSFQRKLKEIFISLILEFRYNKNQ
ILKLYLNNIYLGSGAYGVDEAAKVYFGKFIEELTLSEIALITGLAPAPSIYSPYQNLELAIKNRNKVLESMYVDGYISLA
NKNKAIKEKIKLNYQTADNFLDDKLLINFILEETDKKIGSKNDYEFLRIKSSINKDWQEKGQKISRYAGPKELEFALLSI
ESNTGLIRTMITSKNPSINEYNRVISSVRPLGSTFKIIPYAAALIEGIKLSDKFEDLPICWESYCPKNFSEDYRGSISLI
ESFKSSSNIVPISITKKIGLKNIINLANSFGLGYEQEFEEFPSLAIGSYGDNLLNITNAYSAINNNGKIQSPEIIEKIES
FKKQPIWENKSISTKILDLKINKKINKLLEKSVKEGTSKAASIKGKKIYGKTGTSDGNKDLWFIGSINNLTTGIWIGYDD
NKESELSSGNAAYLWKKFISEIYKIPIKK
>Mature_589_residues
MQKIKFKSFVLIIPILIFSGISFYFFSLISSTLKFDNSKNQRSNVTKYSYVISSSDNKILSKLSRKFEIDNSYHKIPIFL
KHSFISSEDKRFYIHNGIDLKSISRALIQNIRSGYVKEGGSTITQQVARLLFLNNDLSFQRKLKEIFISLILEFRYNKNQ
ILKLYLNNIYLGSGAYGVDEAAKVYFGKFIEELTLSEIALITGLAPAPSIYSPYQNLELAIKNRNKVLESMYVDGYISLA
NKNKAIKEKIKLNYQTADNFLDDKLLINFILEETDKKIGSKNDYEFLRIKSSINKDWQEKGQKISRYAGPKELEFALLSI
ESNTGLIRTMITSKNPSINEYNRVISSVRPLGSTFKIIPYAAALIEGIKLSDKFEDLPICWESYCPKNFSEDYRGSISLI
ESFKSSSNIVPISITKKIGLKNIINLANSFGLGYEQEFEEFPSLAIGSYGDNLLNITNAYSAINNNGKIQSPEIIEKIES
FKKQPIWENKSISTKILDLKINKKINKLLEKSVKEGTSKAASIKGKKIYGKTGTSDGNKDLWFIGSINNLTTGIWIGYDD
NKESELSSGNAAYLWKKFISEIYKIPIKK

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=543, Percent_Identity=24.8618784530387, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI87082258, Length=307, Percent_Identity=33.8762214983713, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1788867, Length=494, Percent_Identity=28.7449392712551, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1789601, Length=137, Percent_Identity=39.4160583941606, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 66947; Mature: 66947

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKIKFKSFVLIIPILIFSGISFYFFSLISSTLKFDNSKNQRSNVTKYSYVISSSDNKIL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCHHHH
SKLSRKFEIDNSYHKIPIFLKHSFISSEDKRFYIHNGIDLKSISRALIQNIRSGYVKEGG
HHHHHHCCCCCCCEEEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCC
STITQQVARLLFLNNDLSFQRKLKEIFISLILEFRYNKNQILKLYLNNIYLGSGAYGVDE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCCCCHH
AAKVYFGKFIEELTLSEIALITGLAPAPSIYSPYQNLELAIKNRNKVLESMYVDGYISLA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCEEEEC
NKNKAIKEKIKLNYQTADNFLDDKLLINFILEETDKKIGSKNDYEFLRIKSSINKDWQEK
CCCHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEHHHCCHHHHHH
GQKISRYAGPKELEFALLSIESNTGLIRTMITSKNPSINEYNRVISSVRPLGSTFKIIPY
HHHHHHHCCCCCEEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHH
AAALIEGIKLSDKFEDLPICWESYCPKNFSEDYRGSISLIESFKSSSNIVPISITKKIGL
HHHHHHCCCCCCCCCCCCCHHHHCCCCCCCHHHCCHHHHHHHHHCCCCEEEEEEHHHHHH
KNIINLANSFGLGYEQEFEEFPSLAIGSYGDNLLNITNAYSAINNNGKIQSPEIIEKIES
HHHHHHHHHCCCCCHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHH
FKKQPIWENKSISTKILDLKINKKINKLLEKSVKEGTSKAASIKGKKIYGKTGTSDGNKD
HHCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHCCCHHHCCCCCEEECCCCCCCCCCC
LWFIGSINNLTTGIWIGYDDNKESELSSGNAAYLWKKFISEIYKIPIKK
EEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQKIKFKSFVLIIPILIFSGISFYFFSLISSTLKFDNSKNQRSNVTKYSYVISSSDNKIL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCHHHH
SKLSRKFEIDNSYHKIPIFLKHSFISSEDKRFYIHNGIDLKSISRALIQNIRSGYVKEGG
HHHHHHCCCCCCCEEEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCC
STITQQVARLLFLNNDLSFQRKLKEIFISLILEFRYNKNQILKLYLNNIYLGSGAYGVDE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCCCCHH
AAKVYFGKFIEELTLSEIALITGLAPAPSIYSPYQNLELAIKNRNKVLESMYVDGYISLA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCEEEEC
NKNKAIKEKIKLNYQTADNFLDDKLLINFILEETDKKIGSKNDYEFLRIKSSINKDWQEK
CCCHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEHHHCCHHHHHH
GQKISRYAGPKELEFALLSIESNTGLIRTMITSKNPSINEYNRVISSVRPLGSTFKIIPY
HHHHHHHCCCCCEEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHH
AAALIEGIKLSDKFEDLPICWESYCPKNFSEDYRGSISLIESFKSSSNIVPISITKKIGL
HHHHHHCCCCCCCCCCCCCHHHHCCCCCCCHHHCCHHHHHHHHHCCCCEEEEEEHHHHHH
KNIINLANSFGLGYEQEFEEFPSLAIGSYGDNLLNITNAYSAINNNGKIQSPEIIEKIES
HHHHHHHHHCCCCCHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHH
FKKQPIWENKSISTKILDLKINKKINKLLEKSVKEGTSKAASIKGKKIYGKTGTSDGNKD
HHCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHCCCHHHCCCCCEEECCCCCCCCCCC
LWFIGSINNLTTGIWIGYDDNKESELSSGNAAYLWKKFISEIYKIPIKK
EEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]