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The map label for this gene is trmH [H]

Identifier: 78778803

GI number: 78778803

Start: 396854

End: 397525

Strand: Reverse

Name: trmH [H]

Synonym: PMT9312_0418

Alternate gene names: 78778803

Gene position: 397525-396854 (Counterclockwise)

Preceding gene: 78778811

Following gene: 78778799

Centisome position: 23.26

GC content: 31.85

Gene sequence:

>672_bases
ATGTCAATTTTGCCAAGAAGATTTGAGCGAATCAAAAGTGTTTTAGATTGCAGAATGCAAAACTTGACTGTTTTAGTTGA
GGATGTCAATAAGCCGCATAATCTATCAGCTATATTAAGAACCTGTGATGCAGCTGGAGTTTTCGAAGCAAATTTTATTA
GCAAAACGAATGCCGTTAAGACTTTTAATAGTACTGCGCAAGGCAGTCAGAAATGGGTCAAATTAAATAATCATGAAAAC
ACTATTACTGCAATATCTGATTTAAAGAATAAGGGTTTTAAATTATATGGAACAACTCTCAATAGTGAATCCGTCGATTA
TAGAAATTTTGATTATTCTCAAAATACATGTTTTGTTTTAGGAGCAGAAAAATGGGGATTAAGTAAAGAAATTATATCTA
TGGTTGATCAATCAATTTTCATACCTATGAGAGGTATGGTTCAATCCTTGAATGTATCAGTTGCTGCCTCCATATTATTA
TTTGAAGCTATTCGTCAGAGAAAAAATAAAGGGAAATTGCCAACAAATGGCGAAGGTTTAAATATGGATGAATATCGAAA
GACCTTGTTTGAGTGGTGTTACCCAGAATTAGCTACTATTTATAAAAAATCAGCTAAAGAATATCCAACGTTGAATGATC
AAGGAAATTTTGATCCTATTAAAGAAAACTAA

Upstream 100 bases:

>100_bases
CATTTAGTTAATTCAAACTACTCAAATATTATAAACCAAGAGATTTTATAGGTAATCAATATTTTTTGAATATAATTTAA
AAAGTGCGAAAAAATTATAA

Downstream 100 bases:

>100_bases
ATTTATTCGGAATTTTGACTATCAAAAATCTCTTCAAGATCCTCCCCTATAAAAGAAAGGCCTAAAACTAAAAAAAACAT
TGCCAAACCTGGGAACAAAG

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSILPRRFERIKSVLDCRMQNLTVLVEDVNKPHNLSAILRTCDAAGVFEANFISKTNAVKTFNSTAQGSQKWVKLNNHEN
TITAISDLKNKGFKLYGTTLNSESVDYRNFDYSQNTCFVLGAEKWGLSKEIISMVDQSIFIPMRGMVQSLNVSVAASILL
FEAIRQRKNKGKLPTNGEGLNMDEYRKTLFEWCYPELATIYKKSAKEYPTLNDQGNFDPIKEN

Sequences:

>Translated_223_residues
MSILPRRFERIKSVLDCRMQNLTVLVEDVNKPHNLSAILRTCDAAGVFEANFISKTNAVKTFNSTAQGSQKWVKLNNHEN
TITAISDLKNKGFKLYGTTLNSESVDYRNFDYSQNTCFVLGAEKWGLSKEIISMVDQSIFIPMRGMVQSLNVSVAASILL
FEAIRQRKNKGKLPTNGEGLNMDEYRKTLFEWCYPELATIYKKSAKEYPTLNDQGNFDPIKEN
>Mature_222_residues
SILPRRFERIKSVLDCRMQNLTVLVEDVNKPHNLSAILRTCDAAGVFEANFISKTNAVKTFNSTAQGSQKWVKLNNHENT
ITAISDLKNKGFKLYGTTLNSESVDYRNFDYSQNTCFVLGAEKWGLSKEIISMVDQSIFIPMRGMVQSLNVSVAASILLF
EAIRQRKNKGKLPTNGEGLNMDEYRKTLFEWCYPELATIYKKSAKEYPTLNDQGNFDPIKEN

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI110825988, Length=155, Percent_Identity=25.8064516129032, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1790083, Length=214, Percent_Identity=42.5233644859813, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI1790623, Length=152, Percent_Identity=35.5263157894737, Blast_Score=81, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21355819, Length=153, Percent_Identity=28.1045751633987, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25338; Mature: 25207

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILPRRFERIKSVLDCRMQNLTVLVEDVNKPHNLSAILRTCDAAGVFEANFISKTNAVK
CCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHCCCHHHH
TFNSTAQGSQKWVKLNNHENTITAISDLKNKGFKLYGTTLNSESVDYRNFDYSQNTCFVL
HCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEE
GAEKWGLSKEIISMVDQSIFIPMRGMVQSLNVSVAASILLFEAIRQRKNKGKLPTNGEGL
ECCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NMDEYRKTLFEWCYPELATIYKKSAKEYPTLNDQGNFDPIKEN
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SILPRRFERIKSVLDCRMQNLTVLVEDVNKPHNLSAILRTCDAAGVFEANFISKTNAVK
CCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHCCCHHHH
TFNSTAQGSQKWVKLNNHENTITAISDLKNKGFKLYGTTLNSESVDYRNFDYSQNTCFVL
HCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEE
GAEKWGLSKEIISMVDQSIFIPMRGMVQSLNVSVAASILLFEAIRQRKNKGKLPTNGEGL
ECCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NMDEYRKTLFEWCYPELATIYKKSAKEYPTLNDQGNFDPIKEN
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]