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The map label for this gene is trmE

Identifier: 78778577

GI number: 78778577

Start: 181580

End: 182962

Strand: Reverse

Name: trmE

Synonym: PMT9312_0191

Alternate gene names: 78778577

Gene position: 182962-181580 (Counterclockwise)

Preceding gene: 78778579

Following gene: 78778576

Centisome position: 10.7

GC content: 27.48

Gene sequence:

>1383_bases
ATGGATTCGATCGTTACTACAGAAGATACCATAGCCGCAATTGCTTCAGCTATAAGTATAGGGAAAGGAGGAGTTGCGAT
AATAAGAGTATCAGGGAAAGACTCAATAAATTCTTGCAAAAAGATTGTTCAAACTAAATCTAAATATGCATGGGAATCAC
ATAGAGTTTTTCATGGTTTTATTCTGGAAAATAAACAAAATAAATTTATAGATGAGGTTTTAATTTTAGTAATGAAATCA
CCAAATAGCTTCACAGGAGAGGATGTTGTTGAACTTCATTGCCATGGAGGAATAATCCTAGTAAATAAAGTTCTACAGAG
ATTATTATCTAGTAATTCTAGAGTTAGACTTGCAAACCCAGGAGAATTTAGTCAAAGAGCTTTTCTTAATGGGAAAATAG
ACCTTACTCAAGCCGAGTCGATTAATCAATTAATTAATGCAAGCAATACCAGATCAGCAGAGCTAGCTTTTAGTGGGGTT
CAAGGGAAAATAAAGAAAAAAATTAATGATATTAAAAATGACCTTATAAATCAACTTTGCGAAATAGAAGCAAGAGTTGA
TTTTGAAGAAGACTTCACAGATTTTGATTACAACAAATATCTAAAAAACATTAAAAAAGTAAAAGAAAAAATAGAATTAC
TAATAGAAAATGCAAAAAGAAATTCATATATTCACAATGGAATATCCATTGCCCTTATAGGTAAAACGAATGTTGGTAAA
AGCTCTTTACTAAATTTGCTTGCAAAAAAAGAGAAGGCAATCGTAACTAATATTCCTGGAACAACTAGAGATGTTATTGA
AGTTAATTTAACTATTAATGATATTCCAATGAAAATAATTGATACTGCTGGCATAAGAGAAACTCATGAACAAATTGAAA
GTATTGGAATTAAAAAAAGTTTTAGGAAAATTAAAGAGTCAGATTTTATAATTTATATTTATAGTCTTGAAGAAGGATTT
AATAAAGAAGACAAAAAAATAATACAAGAAATCCCCAAAGAAAAATTAATTACTATTTTGGGCAATAAAAAAGATTTAAT
TGATTGCAAAAATATTAATTCAAATGAGTTAAAAAACACAATTCTTATGAGCATTAAGAATAATGATGGCGAAAGATTAT
TAATCGACACAATCATAAAAAAATGCGGATTAAAACAAGTAGAAAATATCAATATATTTTTAAACGAAAGACATCTAACA
AATTTGTCTTCTTGCCTATCTAATTTAAATGATACTGATGTAATAATTAAAAATAAATTGCCATTTGATTTGTTATCAAT
AGAACTGAGAGATGGAATTCAAAACTTATCTAAAATAACTGGTCAAGAATTAACAGAGGAACTTCTAGATAATATTTTTT
CTAAGTTTTGTATTGGTAAATAA

Upstream 100 bases:

>100_bases
TTTTCTTATAGGTAATATCTCTTTTAAATCTCATTCAGAAAGAAAATTTCAAAATTAAATTCTTGATAATCTTACTTAAT
TATCCATAACAAAGCTAGAG

Downstream 100 bases:

>100_bases
ATTTGAGTTAAATTACATAGTGATATATAGTTGATTCTCTAACTTCAGTTTAATTTTTATTAATTAATTATTTTTTAGTT
TAGTGGATTTAAATACTCCA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MDSIVTTEDTIAAIASAISIGKGGVAIIRVSGKDSINSCKKIVQTKSKYAWESHRVFHGFILENKQNKFIDEVLILVMKS
PNSFTGEDVVELHCHGGIILVNKVLQRLLSSNSRVRLANPGEFSQRAFLNGKIDLTQAESINQLINASNTRSAELAFSGV
QGKIKKKINDIKNDLINQLCEIEARVDFEEDFTDFDYNKYLKNIKKVKEKIELLIENAKRNSYIHNGISIALIGKTNVGK
SSLLNLLAKKEKAIVTNIPGTTRDVIEVNLTINDIPMKIIDTAGIRETHEQIESIGIKKSFRKIKESDFIIYIYSLEEGF
NKEDKKIIQEIPKEKLITILGNKKDLIDCKNINSNELKNTILMSIKNNDGERLLIDTIIKKCGLKQVENINIFLNERHLT
NLSSCLSNLNDTDVIIKNKLPFDLLSIELRDGIQNLSKITGQELTEELLDNIFSKFCIGK

Sequences:

>Translated_460_residues
MDSIVTTEDTIAAIASAISIGKGGVAIIRVSGKDSINSCKKIVQTKSKYAWESHRVFHGFILENKQNKFIDEVLILVMKS
PNSFTGEDVVELHCHGGIILVNKVLQRLLSSNSRVRLANPGEFSQRAFLNGKIDLTQAESINQLINASNTRSAELAFSGV
QGKIKKKINDIKNDLINQLCEIEARVDFEEDFTDFDYNKYLKNIKKVKEKIELLIENAKRNSYIHNGISIALIGKTNVGK
SSLLNLLAKKEKAIVTNIPGTTRDVIEVNLTINDIPMKIIDTAGIRETHEQIESIGIKKSFRKIKESDFIIYIYSLEEGF
NKEDKKIIQEIPKEKLITILGNKKDLIDCKNINSNELKNTILMSIKNNDGERLLIDTIIKKCGLKQVENINIFLNERHLT
NLSSCLSNLNDTDVIIKNKLPFDLLSIELRDGIQNLSKITGQELTEELLDNIFSKFCIGK
>Mature_460_residues
MDSIVTTEDTIAAIASAISIGKGGVAIIRVSGKDSINSCKKIVQTKSKYAWESHRVFHGFILENKQNKFIDEVLILVMKS
PNSFTGEDVVELHCHGGIILVNKVLQRLLSSNSRVRLANPGEFSQRAFLNGKIDLTQAESINQLINASNTRSAELAFSGV
QGKIKKKINDIKNDLINQLCEIEARVDFEEDFTDFDYNKYLKNIKKVKEKIELLIENAKRNSYIHNGISIALIGKTNVGK
SSLLNLLAKKEKAIVTNIPGTTRDVIEVNLTINDIPMKIIDTAGIRETHEQIESIGIKKSFRKIKESDFIIYIYSLEEGF
NKEDKKIIQEIPKEKLITILGNKKDLIDCKNINSNELKNTILMSIKNNDGERLLIDTIIKKCGLKQVENINIFLNERHLT
NLSSCLSNLNDTDVIIKNKLPFDLLSIELRDGIQNLSKITGQELTEELLDNIFSKFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=464, Percent_Identity=29.0948275862069, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI193082993, Length=454, Percent_Identity=30.1762114537445, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI193082988, Length=496, Percent_Identity=27.4193548387097, Blast_Score=196, Evalue=4e-50,
Organism=Escherichia coli, GI2367268, Length=461, Percent_Identity=33.4056399132321, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI87082120, Length=120, Percent_Identity=32.5, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=460, Percent_Identity=28.4782608695652, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323665, Length=510, Percent_Identity=30.9803921568627, Blast_Score=190, Evalue=3e-49,
Organism=Drosophila melanogaster, GI45550813, Length=474, Percent_Identity=29.746835443038, Blast_Score=196, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 51744; Mature: 51744

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSIVTTEDTIAAIASAISIGKGGVAIIRVSGKDSINSCKKIVQTKSKYAWESHRVFHGF
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCHHHCCEEEEE
ILENKQNKFIDEVLILVMKSPNSFTGEDVVELHCHGGIILVNKVLQRLLSSNSRVRLANP
EEECCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEHHHHHHHHHCCCCEEEECCC
GEFSQRAFLNGKIDLTQAESINQLINASNTRSAELAFSGVQGKIKKKINDIKNDLINQLC
CCCCHHHEECCCEEEHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
EIEARVDFEEDFTDFDYNKYLKNIKKVKEKIELLIENAKRNSYIHNGISIALIGKTNVGK
HHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEEECCCCCH
SSLLNLLAKKEKAIVTNIPGTTRDVIEVNLTINDIPMKIIDTAGIRETHEQIESIGIKKS
HHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEECCCHHHHCCCCCHHHHHHHHHHCHHHH
FRKIKESDFIIYIYSLEEGFNKEDKKIIQEIPKEKLITILGNKKDLIDCKNINSNELKNT
HHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCHHHEEEEECCCCCEEECCCCCHHHHCCE
ILMSIKNNDGERLLIDTIIKKCGLKQVENINIFLNERHLTNLSSCLSNLNDTDVIIKNKL
EEEEEECCCCCEEHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCCEEEECCC
PFDLLSIELRDGIQNLSKITGQELTEELLDNIFSKFCIGK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDSIVTTEDTIAAIASAISIGKGGVAIIRVSGKDSINSCKKIVQTKSKYAWESHRVFHGF
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCHHHCCEEEEE
ILENKQNKFIDEVLILVMKSPNSFTGEDVVELHCHGGIILVNKVLQRLLSSNSRVRLANP
EEECCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEHHHHHHHHHCCCCEEEECCC
GEFSQRAFLNGKIDLTQAESINQLINASNTRSAELAFSGVQGKIKKKINDIKNDLINQLC
CCCCHHHEECCCEEEHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
EIEARVDFEEDFTDFDYNKYLKNIKKVKEKIELLIENAKRNSYIHNGISIALIGKTNVGK
HHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEEECCCCCH
SSLLNLLAKKEKAIVTNIPGTTRDVIEVNLTINDIPMKIIDTAGIRETHEQIESIGIKKS
HHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEECCCHHHHCCCCCHHHHHHHHHHCHHHH
FRKIKESDFIIYIYSLEEGFNKEDKKIIQEIPKEKLITILGNKKDLIDCKNINSNELKNT
HHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCHHHEEEEECCCCCEEECCCCCHHHHCCE
ILMSIKNNDGERLLIDTIIKKCGLKQVENINIFLNERHLTNLSSCLSNLNDTDVIIKNKL
EEEEEECCCCCEEHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCCEEEECCC
PFDLLSIELRDGIQNLSKITGQELTEELLDNIFSKFCIGK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA