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The map label for this gene is cca [H]

Identifier: 78778529

GI number: 78778529

Start: 138356

End: 139576

Strand: Direct

Name: cca [H]

Synonym: PMT9312_0143

Alternate gene names: 78778529

Gene position: 138356-139576 (Clockwise)

Preceding gene: 78778526

Following gene: 78778530

Centisome position: 8.09

GC content: 26.54

Gene sequence:

>1221_bases
ATGAAGGGTAATAACGTTCTTACTGATCATACACTAATCATTGATGAATTAGAAAGAAGTATAAAATTTTATAATTGGCA
TTTTATCTTATCTTTTCTACCTAAAGGATCATTTTTAGTTGGTGGTTACATAAGGGATATTATTTTGGGAAGATTAACTG
AAGATGTAGATGTTGATATTGTGGTACCCCTAAATGCTATTGAAATTGGGAAAAAAATTTCAGACAATTTCGAGTCTAAA
TTTATAATTTTAGATAAGAAAAGAGAAGTGGTAAGGGTTATTCTTGATCATATTTCAATTGATATTGCTAATCAGATTTC
CTCCACGATCGAAGGAGATTTGAGTAGTAGGGACTTCTCGATTAATTCAATTGCTTTTTTATTTGATAATAAATGTTTGT
TTGATCCATTTAATGGTCTAAAAGATCTAGAATCTTCCTTGCTGAGAACTCATTCAGAAAAAAATTTAATGAATGATCCT
TTACGAATATTAAGATGTTTTCGATTTGTTTCTGAATTTAATTTTAAAATTGATCAAGATTTAATAACTTATATAAAAAA
AAATAAAGGGAAATTATATTTTGTTGCCAAAGAAAGGATTAATTATGAAATTCAGAAGATAGTAACTGGGGCAAATGCTT
TAGAGGCGATAATTTTAGTAAAAAATTTTAATATTTTTGGATCGGATAATTTATACAAAGATTCATTTTTTTTGGATTTA
CAGAATATTAATTATGAAGAACTTAATCAGTATGAAAAGAAGAATTTTTTACCATTTTTTTTTATTACCCAAATTTTAGA
TGAAGTATCTTTAAGTAAATATAAATTTAGTAGAGCTGAAATTGTAAGAACAAAGTTATTGCGAAAATGGCACGTTTTTT
TAAAGAAAAAAAATATCACTCAGTTAAATGAATTAGATAGATTTGCATTACATCAAGAATTAGAAATGTTTCTTCCATCT
TTTATTTTTTATTTACCTCAAAAATTACGTTTCGATTGGCTCAATAGGTGGCGGGACAAGGATGATAGATTATTTCATCC
TTCAAATTTAATTAATGGTGATGTAATCAAAAAAAATTTAAAAATAAAGGATGGCCCTCTGTTAGGCAAGCTTTTACAAT
ATCTTTCAAAGGAGCTTGCATATAAAAGATTGAATAATTTTGATGAAGCTATTTATAAAGCAAAGCAATGGATTGAACAA
AATGCGCCAAAATGTGATTAA

Upstream 100 bases:

>100_bases
CAAATATTATCAACACCATGCTTATTTTCAACGTCATGATATGTGATACATCTATCAACCCATTTACAGTTGATGCAAAT
GCACATAATTAATAAATAAA

Downstream 100 bases:

>100_bases
ATACACATAGCTTAGTTTTGTTTAGAAGTTCAGACTTCAAAAATCATTTTTAAAAATTTATGAGTATTCGAATTTACATT
GGCAATTTACCACAAGGATT

Product: poly A polymerase family protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MKGNNVLTDHTLIIDELERSIKFYNWHFILSFLPKGSFLVGGYIRDIILGRLTEDVDVDIVVPLNAIEIGKKISDNFESK
FIILDKKREVVRVILDHISIDIANQISSTIEGDLSSRDFSINSIAFLFDNKCLFDPFNGLKDLESSLLRTHSEKNLMNDP
LRILRCFRFVSEFNFKIDQDLITYIKKNKGKLYFVAKERINYEIQKIVTGANALEAIILVKNFNIFGSDNLYKDSFFLDL
QNINYEELNQYEKKNFLPFFFITQILDEVSLSKYKFSRAEIVRTKLLRKWHVFLKKKNITQLNELDRFALHQELEMFLPS
FIFYLPQKLRFDWLNRWRDKDDRLFHPSNLINGDVIKKNLKIKDGPLLGKLLQYLSKELAYKRLNNFDEAIYKAKQWIEQ
NAPKCD

Sequences:

>Translated_406_residues
MKGNNVLTDHTLIIDELERSIKFYNWHFILSFLPKGSFLVGGYIRDIILGRLTEDVDVDIVVPLNAIEIGKKISDNFESK
FIILDKKREVVRVILDHISIDIANQISSTIEGDLSSRDFSINSIAFLFDNKCLFDPFNGLKDLESSLLRTHSEKNLMNDP
LRILRCFRFVSEFNFKIDQDLITYIKKNKGKLYFVAKERINYEIQKIVTGANALEAIILVKNFNIFGSDNLYKDSFFLDL
QNINYEELNQYEKKNFLPFFFITQILDEVSLSKYKFSRAEIVRTKLLRKWHVFLKKKNITQLNELDRFALHQELEMFLPS
FIFYLPQKLRFDWLNRWRDKDDRLFHPSNLINGDVIKKNLKIKDGPLLGKLLQYLSKELAYKRLNNFDEAIYKAKQWIEQ
NAPKCD
>Mature_406_residues
MKGNNVLTDHTLIIDELERSIKFYNWHFILSFLPKGSFLVGGYIRDIILGRLTEDVDVDIVVPLNAIEIGKKISDNFESK
FIILDKKREVVRVILDHISIDIANQISSTIEGDLSSRDFSINSIAFLFDNKCLFDPFNGLKDLESSLLRTHSEKNLMNDP
LRILRCFRFVSEFNFKIDQDLITYIKKNKGKLYFVAKERINYEIQKIVTGANALEAIILVKNFNIFGSDNLYKDSFFLDL
QNINYEELNQYEKKNFLPFFFITQILDEVSLSKYKFSRAEIVRTKLLRKWHVFLKKKNITQLNELDRFALHQELEMFLPS
FIFYLPQKLRFDWLNRWRDKDDRLFHPSNLINGDVIKKNLKIKDGPLLGKLLQYLSKELAYKRLNNFDEAIYKAKQWIEQ
NAPKCD

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 47941; Mature: 47941

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGNNVLTDHTLIIDELERSIKFYNWHFILSFLPKGSFLVGGYIRDIILGRLTEDVDVDI
CCCCCEECCHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEHHHHHHHHHHHCCCCCCEEE
VVPLNAIEIGKKISDNFESKFIILDKKREVVRVILDHISIDIANQISSTIEGDLSSRDFS
EEECCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
INSIAFLFDNKCLFDPFNGLKDLESSLLRTHSEKNLMNDPLRILRCFRFVSEFNFKIDQD
CCEEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHH
LITYIKKNKGKLYFVAKERINYEIQKIVTGANALEAIILVKNFNIFGSDNLYKDSFFLDL
HHHHHHCCCCCEEEEEHHHHCHHHHHHHHCCHHHHEEEEEEECCEECCCCCCCCEEEEEE
QNINYEELNQYEKKNFLPFFFITQILDEVSLSKYKFSRAEIVRTKLLRKWHVFLKKKNIT
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
QLNELDRFALHQELEMFLPSFIFYLPQKLRFDWLNRWRDKDDRLFHPSNLINGDVIKKNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHCCC
KIKDGPLLGKLLQYLSKELAYKRLNNFDEAIYKAKQWIEQNAPKCD
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKGNNVLTDHTLIIDELERSIKFYNWHFILSFLPKGSFLVGGYIRDIILGRLTEDVDVDI
CCCCCEECCHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEHHHHHHHHHHHCCCCCCEEE
VVPLNAIEIGKKISDNFESKFIILDKKREVVRVILDHISIDIANQISSTIEGDLSSRDFS
EEECCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
INSIAFLFDNKCLFDPFNGLKDLESSLLRTHSEKNLMNDPLRILRCFRFVSEFNFKIDQD
CCEEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHH
LITYIKKNKGKLYFVAKERINYEIQKIVTGANALEAIILVKNFNIFGSDNLYKDSFFLDL
HHHHHHCCCCCEEEEEHHHHCHHHHHHHHCCHHHHEEEEEEECCEECCCCCCCCEEEEEE
QNINYEELNQYEKKNFLPFFFITQILDEVSLSKYKFSRAEIVRTKLLRKWHVFLKKKNIT
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
QLNELDRFALHQELEMFLPSFIFYLPQKLRFDWLNRWRDKDDRLFHPSNLINGDVIKKNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHCCC
KIKDGPLLGKLLQYLSKELAYKRLNNFDEAIYKAKQWIEQNAPKCD
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA