Click here to switch to the map view.

The map label for this gene is queD [H]

Identifier: 78778495

GI number: 78778495

Start: 111407

End: 112333

Strand: Reverse

Name: queD [H]

Synonym: PMT9312_0110

Alternate gene names: 78778495

Gene position: 112333-111407 (Counterclockwise)

Preceding gene: 78778497

Following gene: 78778494

Centisome position: 6.57

GC content: 34.2

Gene sequence:

>927_bases
ATGACTTCTACACAATCCAAACCATTACATGGAAAAGGACGTGAATGCGTCATAACTCGGCGTGCCTGCTTTAGTTCTAG
TCACCGTTATTGGCTTCCTGAAAAAAGCCCAGAAGAAAATTTATCTCTTTTTGGAAAGTGCAGTATTGCACCAGGTCATG
GTCATAATTATGAACTTATTGTTTCAATGGGTGGAGAACTAGACTCTGATGGAATGGTACTTAATCTCTCTGATGTAAAA
CACTCTATTAAAGATAAGGTTACTGGACAATTAGATTTTCGTTTTTTAAATGATGTCTGGCCTGAATTTAATGTTAATAA
TCAAGAGGGTTTACTTCCCACAACTGAAGCATTAGTAAAGGTCATTTGGAGTCGTCTAAAGGATGATTTACCTCTTACAA
GTCTAAGGCTTTATGAAAACCCAAATTTATGGGCAGATTATTTTGGAAAAAACATGGAAGCATTTTTAACAGTACAAACT
CATTTTGCAGCCGCTCATAGACTTGCAAAAGAAGAGATATCATTTGATGAAAATAAAAAAATCTATGGGAAATGTGCCAG
AGTTAATGGACATGGTCACAACTATCTTGTCGATATAACTGTAAAAGGAGACATTGATAAAAGAACAGGAATGGTTTGCG
ACTTATCTGCCCTCCAAGAGATAATTAATGATTTGGTTGTTGAACAACTAGATCATACTTTTCTCAATAAAGATATCGAA
TTTTTCCATAGTTGTGTTCCAACTGCTGAAAATATAGCTTTATATATTTCTGACATTCTTAAAAAACCAATACATAACCT
TGGTGCAACATTACACAAAATAAGACTCCAAGAGAGCCCAAATAATGCTGCAGAAATTTATGTTGATCAAAAGTTAACCA
ATTCATTGCAGTTGAAATTTGAAAATAGTTTAGTCACACAAACTTGA

Upstream 100 bases:

>100_bases
CTTCTACCCTTGATAACCTTAACTGTTTTTTCGATAATGGATTGTTCCATAAAGACAAAAGCGTATTTAAAACCTTAAGA
TAGTATTATCGCAGACAGGT

Downstream 100 bases:

>100_bases
GTATCCCAAGAGTAATAATTGTTATAGCATCTAGTCTTGATGGAAGAATTGCATTTCCTGAAGGTGGAGAATCGCATCTT
GGAAGCGAAGAAGATAAAAA

Product: 6-pyruvoyl tetrahydropterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: CPH4 synthase; Archaeosine biosynthesis protein queD [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTSTQSKPLHGKGRECVITRRACFSSSHRYWLPEKSPEENLSLFGKCSIAPGHGHNYELIVSMGGELDSDGMVLNLSDVK
HSIKDKVTGQLDFRFLNDVWPEFNVNNQEGLLPTTEALVKVIWSRLKDDLPLTSLRLYENPNLWADYFGKNMEAFLTVQT
HFAAAHRLAKEEISFDENKKIYGKCARVNGHGHNYLVDITVKGDIDKRTGMVCDLSALQEIINDLVVEQLDHTFLNKDIE
FFHSCVPTAENIALYISDILKKPIHNLGATLHKIRLQESPNNAAEIYVDQKLTNSLQLKFENSLVTQT

Sequences:

>Translated_308_residues
MTSTQSKPLHGKGRECVITRRACFSSSHRYWLPEKSPEENLSLFGKCSIAPGHGHNYELIVSMGGELDSDGMVLNLSDVK
HSIKDKVTGQLDFRFLNDVWPEFNVNNQEGLLPTTEALVKVIWSRLKDDLPLTSLRLYENPNLWADYFGKNMEAFLTVQT
HFAAAHRLAKEEISFDENKKIYGKCARVNGHGHNYLVDITVKGDIDKRTGMVCDLSALQEIINDLVVEQLDHTFLNKDIE
FFHSCVPTAENIALYISDILKKPIHNLGATLHKIRLQESPNNAAEIYVDQKLTNSLQLKFENSLVTQT
>Mature_307_residues
TSTQSKPLHGKGRECVITRRACFSSSHRYWLPEKSPEENLSLFGKCSIAPGHGHNYELIVSMGGELDSDGMVLNLSDVKH
SIKDKVTGQLDFRFLNDVWPEFNVNNQEGLLPTTEALVKVIWSRLKDDLPLTSLRLYENPNLWADYFGKNMEAFLTVQTH
FAAAHRLAKEEISFDENKKIYGKCARVNGHGHNYLVDITVKGDIDKRTGMVCDLSALQEIINDLVVEQLDHTFLNKDIEF
FHSCVPTAENIALYISDILKKPIHNLGATLHKIRLQESPNNAAEIYVDQKLTNSLQLKFENSLVTQT

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde [H]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily [H]

Homologues:

Organism=Homo sapiens, GI4506331, Length=136, Percent_Identity=40.4411764705882, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI115532226, Length=121, Percent_Identity=43.801652892562, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI115532224, Length=121, Percent_Identity=43.801652892562, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19550074, Length=133, Percent_Identity=44.3609022556391, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24585378, Length=133, Percent_Identity=44.3609022556391, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007115 [H]

Pfam domain/function: PF01242 PTPS [H]

EC number: 4.2.3.12

Molecular weight: Translated: 34870; Mature: 34739

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTQSKPLHGKGRECVITRRACFSSSHRYWLPEKSPEENLSLFGKCSIAPGHGHNYELI
CCCCCCCCCCCCCCEEEEEEHHHHCCCCCEECCCCCCCCCCEEEEEEEECCCCCCCEEEE
VSMGGELDSDGMVLNLSDVKHSIKDKVTGQLDFRFLNDVWPEFNVNNQEGLLPTTEALVK
EEECCCCCCCCEEEEHHHHHHHHHHHCCCEEHEEEHHHCCCCCCCCCCCCCCCHHHHHHH
VIWSRLKDDLPLTSLRLYENPNLWADYFGKNMEAFLTVQTHFAAAHRLAKEEISFDENKK
HHHHHHHCCCCCEEEEEECCCCCHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCE
IYGKCARVNGHGHNYLVDITVKGDIDKRTGMVCDLSALQEIINDLVVEQLDHTFLNKDIE
EEEEEEEECCCCCEEEEEEEEECCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHH
FFHSCVPTAENIALYISDILKKPIHNLGATLHKIRLQESPNNAAEIYVDQKLTNSLQLKF
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEEEECCCCCCEEEEE
ENSLVTQT
CCCCCCCC
>Mature Secondary Structure 
TSTQSKPLHGKGRECVITRRACFSSSHRYWLPEKSPEENLSLFGKCSIAPGHGHNYELI
CCCCCCCCCCCCCEEEEEEHHHHCCCCCEECCCCCCCCCCEEEEEEEECCCCCCCEEEE
VSMGGELDSDGMVLNLSDVKHSIKDKVTGQLDFRFLNDVWPEFNVNNQEGLLPTTEALVK
EEECCCCCCCCEEEEHHHHHHHHHHHCCCEEHEEEHHHCCCCCCCCCCCCCCCHHHHHHH
VIWSRLKDDLPLTSLRLYENPNLWADYFGKNMEAFLTVQTHFAAAHRLAKEEISFDENKK
HHHHHHHCCCCCEEEEEECCCCCHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCE
IYGKCARVNGHGHNYLVDITVKGDIDKRTGMVCDLSALQEIINDLVVEQLDHTFLNKDIE
EEEEEEEECCCCCEEEEEEEEECCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHH
FFHSCVPTAENIALYISDILKKPIHNLGATLHKIRLQESPNNAAEIYVDQKLTNSLQLKF
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEEEECCCCCCEEEEE
ENSLVTQT
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]