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The map label for this gene is rimO [H]

Identifier: 78778491

GI number: 78778491

Start: 107562

End: 108977

Strand: Direct

Name: rimO [H]

Synonym: PMT9312_0106

Alternate gene names: 78778491

Gene position: 107562-108977 (Clockwise)

Preceding gene: 78778489

Following gene: 78778496

Centisome position: 6.29

GC content: 31.85

Gene sequence:

>1416_bases
ATGAGAGTTATAAACGGAATGCCGTTTGAATTTTTTAATTTTTTAAAGTTTGTGAAACAAAATAATCTAAATGTTAAAGA
AAAAAAATTCTCTAAAGTTGCTTTTAGTCATGTTGGTTGTGAGAAAAATCTAGTCGATACTCAACATATGCAAGGCTTAT
TAGATAAAGATGGTTATGAAGTTGAAAGTAATATAAACGATGCAAATATTGTTGTTGTCAATACTTGTAGTTTTATTGAA
ACAGCTAGAGAAGAATCAATTAGGAAAATTCTAGAATACACAAATCAAGGAAAAGAAGTAATTGTTGCAGGCTGTATGGC
TCAGCATTTTAAAGAAGAACTTCTAAAAGAAATACCCGAAATAAAAGGTTTGGTTGGAACTGGAGATTATCAAAAGATAG
CCAAGGTTTTAGATAGAGTAGAAAAAGGGGAAATCGTTAATGAAGTTTCAAAAATACCTGAATTTATTGCAGATGAGGAA
ATACCTCGTTTTGTAGATAAAAACAAATTCGTTGCTTATCTTCGTATTGCTGAAGGCTGCAACTATAATTGCGCTTTTTG
TATTATTCCTAAGTTGAGAGGTCCTCAAAGAAGTAGGACAATAGAATCTATAGTTTCAGAAGCCAAAAGTCTTGCAAAGC
AGGGTATTAAAGAAATCATATTAATTAGTCAAATTACAACTAATTACGGAAAAGATATTTATGGAAAACCATCATTAGCC
AAACTTTTGAATGAGCTTTCTAAAGTTCCAATTCCTTGGATAAGGATACATTATGCGTATCCAACGGGTTTAACTGATGA
AGTTATTAGAGCTTTCAAAGATTCAAAGAATATAGTGCCTTACTTTGATTTGCCACTTCAACATAGTCATCCAGATGTGT
TGAAGAGTATGAACAGACCTTGGCAGGCTTCTTTGAATGAATCAATTTTGGAGAAAATTAGAGAAGAAATTCCATCTGCT
GTATTAAGAACTAGTCTCATTGTTGGTTTCCCAGGAGAAAAAAAAGAACATTTTGAACATCTTCTCGAATTTTTGGATAG
GCACAAATTTGATCATGTGGGAGTGTTTATTTTTTCTCCTGAGGAAGGAACTGCAGCTTTTGATTTACCAAATAAAGTAT
CCCCAGAGGTTGCAGCGGCAAGAAAAGATAACGTTATTTCAGTGCAGCAAAATATCTCTAAAGATAAAAATCAGTCATAT
GTTGGCTCAAAAATGAAGATTTTGGTGGAAAAAATATCAGATAATAACGAATTAATAGGTCGGTCCTACAATTTCGCGCC
TGAAATTGACGGAAATGTGATTTTATCTATTAGTGCTAATAATTATTTGAGAAATTATATTGGTAAATTTGTTGAAGCAA
ATATTTCTTTTGCGGATGAATATGATTTGTATGGAGAGACTATTAAAATTTTGTAG

Upstream 100 bases:

>100_bases
GGCCATTTTAGATCCTTTTTATTTCTACTTTTTAAAGTCTTAAGCGCCATAAAAAATGAAAGTTTGTTATTTACATGATC
TGTCCTAATATTATCTTGGC

Downstream 100 bases:

>100_bases
TTTTTTTAAATTAATTTAACTGCTCTTAAGAGCTTATCTAATGCGAAAGCAGCTCCAAAACCAAAACCAATAAATGCAAA
ATAGAAAATGATGTTCATTA

Product: hypothetical protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MRVINGMPFEFFNFLKFVKQNNLNVKEKKFSKVAFSHVGCEKNLVDTQHMQGLLDKDGYEVESNINDANIVVVNTCSFIE
TAREESIRKILEYTNQGKEVIVAGCMAQHFKEELLKEIPEIKGLVGTGDYQKIAKVLDRVEKGEIVNEVSKIPEFIADEE
IPRFVDKNKFVAYLRIAEGCNYNCAFCIIPKLRGPQRSRTIESIVSEAKSLAKQGIKEIILISQITTNYGKDIYGKPSLA
KLLNELSKVPIPWIRIHYAYPTGLTDEVIRAFKDSKNIVPYFDLPLQHSHPDVLKSMNRPWQASLNESILEKIREEIPSA
VLRTSLIVGFPGEKKEHFEHLLEFLDRHKFDHVGVFIFSPEEGTAAFDLPNKVSPEVAAARKDNVISVQQNISKDKNQSY
VGSKMKILVEKISDNNELIGRSYNFAPEIDGNVILSISANNYLRNYIGKFVEANISFADEYDLYGETIKIL

Sequences:

>Translated_471_residues
MRVINGMPFEFFNFLKFVKQNNLNVKEKKFSKVAFSHVGCEKNLVDTQHMQGLLDKDGYEVESNINDANIVVVNTCSFIE
TAREESIRKILEYTNQGKEVIVAGCMAQHFKEELLKEIPEIKGLVGTGDYQKIAKVLDRVEKGEIVNEVSKIPEFIADEE
IPRFVDKNKFVAYLRIAEGCNYNCAFCIIPKLRGPQRSRTIESIVSEAKSLAKQGIKEIILISQITTNYGKDIYGKPSLA
KLLNELSKVPIPWIRIHYAYPTGLTDEVIRAFKDSKNIVPYFDLPLQHSHPDVLKSMNRPWQASLNESILEKIREEIPSA
VLRTSLIVGFPGEKKEHFEHLLEFLDRHKFDHVGVFIFSPEEGTAAFDLPNKVSPEVAAARKDNVISVQQNISKDKNQSY
VGSKMKILVEKISDNNELIGRSYNFAPEIDGNVILSISANNYLRNYIGKFVEANISFADEYDLYGETIKIL
>Mature_471_residues
MRVINGMPFEFFNFLKFVKQNNLNVKEKKFSKVAFSHVGCEKNLVDTQHMQGLLDKDGYEVESNINDANIVVVNTCSFIE
TAREESIRKILEYTNQGKEVIVAGCMAQHFKEELLKEIPEIKGLVGTGDYQKIAKVLDRVEKGEIVNEVSKIPEFIADEE
IPRFVDKNKFVAYLRIAEGCNYNCAFCIIPKLRGPQRSRTIESIVSEAKSLAKQGIKEIILISQITTNYGKDIYGKPSLA
KLLNELSKVPIPWIRIHYAYPTGLTDEVIRAFKDSKNIVPYFDLPLQHSHPDVLKSMNRPWQASLNESILEKIREEIPSA
VLRTSLIVGFPGEKKEHFEHLLEFLDRHKFDHVGVFIFSPEEGTAAFDLPNKVSPEVAAARKDNVISVQQNISKDKNQSY
VGSKMKILVEKISDNNELIGRSYNFAPEIDGNVILSISANNYLRNYIGKFVEANISFADEYDLYGETIKIL

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=442, Percent_Identity=29.6380090497738, Blast_Score=175, Evalue=9e-44,
Organism=Homo sapiens, GI28872782, Length=441, Percent_Identity=29.7052154195011, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI93277076, Length=389, Percent_Identity=24.6786632390745, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1787057, Length=449, Percent_Identity=39.1982182628062, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI1786882, Length=454, Percent_Identity=27.7533039647577, Blast_Score=166, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=470, Percent_Identity=25.1063829787234, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI71996771, Length=332, Percent_Identity=25.6024096385542, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356207, Length=484, Percent_Identity=25.6198347107438, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=24.6696035242291, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 53502; Mature: 53502

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVINGMPFEFFNFLKFVKQNNLNVKEKKFSKVAFSHVGCEKNLVDTQHMQGLLDKDGYE
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEE
VESNINDANIVVVNTCSFIETAREESIRKILEYTNQGKEVIVAGCMAQHFKEELLKEIPE
EECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHH
IKGLVGTGDYQKIAKVLDRVEKGEIVNEVSKIPEFIADEEIPRFVDKNKFVAYLRIAEGC
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEEECCC
NYNCAFCIIPKLRGPQRSRTIESIVSEAKSLAKQGIKEIILISQITTNYGKDIYGKPSLA
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
KLLNELSKVPIPWIRIHYAYPTGLTDEVIRAFKDSKNIVPYFDLPLQHSHPDVLKSMNRP
HHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHCCCC
WQASLNESILEKIREEIPSAVLRTSLIVGFPGEKKEHFEHLLEFLDRHKFDHVGVFIFSP
CHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECC
EEGTAAFDLPNKVSPEVAAARKDNVISVQQNISKDKNQSYVGSKMKILVEKISDNNELIG
CCCCEEECCCCCCCCHHHHHCCCCEEEHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEC
RSYNFAPEIDGNVILSISANNYLRNYIGKFVEANISFADEYDLYGETIKIL
CCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MRVINGMPFEFFNFLKFVKQNNLNVKEKKFSKVAFSHVGCEKNLVDTQHMQGLLDKDGYE
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEE
VESNINDANIVVVNTCSFIETAREESIRKILEYTNQGKEVIVAGCMAQHFKEELLKEIPE
EECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHH
IKGLVGTGDYQKIAKVLDRVEKGEIVNEVSKIPEFIADEEIPRFVDKNKFVAYLRIAEGC
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEEECCC
NYNCAFCIIPKLRGPQRSRTIESIVSEAKSLAKQGIKEIILISQITTNYGKDIYGKPSLA
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
KLLNELSKVPIPWIRIHYAYPTGLTDEVIRAFKDSKNIVPYFDLPLQHSHPDVLKSMNRP
HHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHCCCC
WQASLNESILEKIREEIPSAVLRTSLIVGFPGEKKEHFEHLLEFLDRHKFDHVGVFIFSP
CHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECC
EEGTAAFDLPNKVSPEVAAARKDNVISVQQNISKDKNQSYVGSKMKILVEKISDNNELIG
CCCCEEECCCCCCCCHHHHHCCCCEEEHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEC
RSYNFAPEIDGNVILSISANNYLRNYIGKFVEANISFADEYDLYGETIKIL
CCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA