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The map label for this gene is grpE

Identifier: 78778401

GI number: 78778401

Start: 18338

End: 19057

Strand: Direct

Name: grpE

Synonym: PMT9312_0016

Alternate gene names: 78778401

Gene position: 18338-19057 (Clockwise)

Preceding gene: 78778400

Following gene: 78778402

Centisome position: 1.07

GC content: 32.22

Gene sequence:

>720_bases
ATGATTGAAAATCAATCAGACAATATTGATATTAAAGAAAATGATGTTTTGAATCAGGATAATGCCCCTGAGGATAATTC
ATCTGCTGCAGAAAAAACAATCGAAAATGATGAATTATCTCCACAAAAAACAGAAGAAATAAATACCGAAGAATTAAAAA
ATACTATTTCCAATAATGATGCAAGGTTAGAACAGTTAGAAAAAGAGCACGAAACATTAAAAAATCAATATGTAAGAATT
TCAGCAGATTTTGATAATTTTAGAAAAAGGCAGTCTAGAGATCAAGACGATTTAAAAGTCCAACTTGTTTCTAAAACTTT
AACTGCAATACTCCCTATTGTTGATAATTTTGAGAGAGCAAGACAGCAACTTAAACCAGAAAGTGAAGAAGCTCAAGCCC
TTCATAGAAGTTATCAAGGATTGTATAAACAATTGGTAGAAGTTTTAAAACAACAAGGAGTTTCGCCGATGAGAGTTGTT
GGTCAACAATTTGATCCAAATTTACATGAAGCCGTATTAAGAGAGCCTAGTGAAGAGTTTAAAGAAGATTTGATTGTAGA
AGAATTGCAGCGAGGATATCATTTAGAGGGAAAGGTTTTGAGACATGCTTTGGTTAAAGTTTCTATGGGACATGGTCAAC
AAAATTCACAAGAGGAAGTAGAAAAGGATACAGTTGAAGAGGATATTGATTCAGAGGAAAATACTTCTGAAGATGTATAA

Upstream 100 bases:

>100_bases
AGCATTAAGGTTTGTTCCTGAGTAAAAATTTGTGCGGCCTTCCGCAACTTGTTTTGTGCCTCACTTTATTAGAAAATAGT
TTTATTAAATTTTTAGAAAA

Downstream 100 bases:

>100_bases
ATTCCTAATTTTTACTTGAGCATTATCTAATGGCTGATTTTTACCAAATACTTGGAGTTTCTCGAGATGCTGATGCGGAT
ACCTTAAAAAGGGCTTATAG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MIENQSDNIDIKENDVLNQDNAPEDNSSAAEKTIENDELSPQKTEEINTEELKNTISNNDARLEQLEKEHETLKNQYVRI
SADFDNFRKRQSRDQDDLKVQLVSKTLTAILPIVDNFERARQQLKPESEEAQALHRSYQGLYKQLVEVLKQQGVSPMRVV
GQQFDPNLHEAVLREPSEEFKEDLIVEELQRGYHLEGKVLRHALVKVSMGHGQQNSQEEVEKDTVEEDIDSEENTSEDV

Sequences:

>Translated_239_residues
MIENQSDNIDIKENDVLNQDNAPEDNSSAAEKTIENDELSPQKTEEINTEELKNTISNNDARLEQLEKEHETLKNQYVRI
SADFDNFRKRQSRDQDDLKVQLVSKTLTAILPIVDNFERARQQLKPESEEAQALHRSYQGLYKQLVEVLKQQGVSPMRVV
GQQFDPNLHEAVLREPSEEFKEDLIVEELQRGYHLEGKVLRHALVKVSMGHGQQNSQEEVEKDTVEEDIDSEENTSEDV
>Mature_239_residues
MIENQSDNIDIKENDVLNQDNAPEDNSSAAEKTIENDELSPQKTEEINTEELKNTISNNDARLEQLEKEHETLKNQYVRI
SADFDNFRKRQSRDQDDLKVQLVSKTLTAILPIVDNFERARQQLKPESEEAQALHRSYQGLYKQLVEVLKQQGVSPMRVV
GQQFDPNLHEAVLREPSEEFKEDLIVEELQRGYHLEGKVLRHALVKVSMGHGQQNSQEEVEKDTVEEDIDSEENTSEDV

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=135, Percent_Identity=23.7037037037037, Blast_Score=63, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24653432, Length=140, Percent_Identity=30.7142857142857, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_PROM9 (Q31DG8)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_396513.1
- ProteinModelPortal:   Q31DG8
- SMR:   Q31DG8
- STRING:   Q31DG8
- GeneID:   3764797
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_0016
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NDARLEQ
- ProtClustDB:   CLSK921706
- BioCyc:   PMAR74546:PMT9312_0016-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 27568; Mature: 27568

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIENQSDNIDIKENDVLNQDNAPEDNSSAAEKTIENDELSPQKTEEINTEELKNTISNND
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHCCCHHHHHHHHCCCH
ARLEQLEKEHETLKNQYVRISADFDNFRKRQSRDQDDLKVQLVSKTLTAILPIVDNFERA
HHHHHHHHHHHHHHHHHEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RQQLKPESEEAQALHRSYQGLYKQLVEVLKQQGVSPMRVVGQQFDPNLHEAVLREPSEEF
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCHHHH
KEDLIVEELQRGYHLEGKVLRHALVKVSMGHGQQNSQEEVEKDTVEEDIDSEENTSEDV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIENQSDNIDIKENDVLNQDNAPEDNSSAAEKTIENDELSPQKTEEINTEELKNTISNND
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHCCCHHHHHHHHCCCH
ARLEQLEKEHETLKNQYVRISADFDNFRKRQSRDQDDLKVQLVSKTLTAILPIVDNFERA
HHHHHHHHHHHHHHHHHEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RQQLKPESEEAQALHRSYQGLYKQLVEVLKQQGVSPMRVVGQQFDPNLHEAVLREPSEEF
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCHHHH
KEDLIVEELQRGYHLEGKVLRHALVKVSMGHGQQNSQEEVEKDTVEEDIDSEENTSEDV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA