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The map label for this gene is pcrA [H]

Identifier: 78358610

GI number: 78358610

Start: 3527207

End: 3529366

Strand: Reverse

Name: pcrA [H]

Synonym: Dde_3571

Alternate gene names: 78358610

Gene position: 3529366-3527207 (Counterclockwise)

Preceding gene: 78358611

Following gene: 78358609

Centisome position: 94.62

GC content: 58.75

Gene sequence:

>2160_bases
ATGATTGATTATAAAAAAGAACTGAACGCGGCTCAGTACGAAGCCGCCACCACCCTCGAAGGCCCCATGCTGGTCATAGC
CGGAGCAGGCAGCGGTAAAACCCGCACAGTGGTGTACCGGCTGGCAAACATGGTGCAGCAGGGCATTGCCGCGTCCAATA
TTCTTCTGCTTACGTTCACCCGCAAGGCCGCACAGGAAATGCAGCACAGAGCGGCCCAGCTGCTGGGCCAGTCCGCGGGC
ATGGTGGGCGGAGTGCAGGGAGGTACGTTCCATGCCTTTGCCTATTCCATTTTGCGCATGCACAAACCGCAGGGGTACGA
AGGCGATCTGTCCGTCATGGATTCCGCCGATGCCGCCGGTGCGCTGAAACACTGCAAGGATACGCTGGGACTGGGCAAAG
GGGACCGGAGTTTTCCCAAAACGCAGACCGTCATGGGGCTGCTGTCCAAAGCGCGCAACAAGGAACTGACGGTGGAGGAT
GTCCTGCGGCGCGAAGCCTTTCACCTGGCATCCTACGCGGGCGATCTTACGCGCATCAGCGACACCTACAGGGCGTTCAA
GCGCAGACATGCGCTTATGGATTATGATGACCTGCTGTTTGAGCTGGAAAGGCTGCTGCAGTCCGATGCCGACCTGCTGG
CGTACTACCGCGACCGGTTCAGATATATCATGGTGGACGAATTTCAGGACACCAACCTGGTACAGGCGCGGTTGGTGCGT
TTGCTGGGCAGCGGCCACAACAACGTGATGGCTGTGGGCGACGACGCGCAGTCCATCTACGCGTTCCGCGGTGCCAATGT
GCAGAATATTCTTGATTTTCCTGACATGTTTCCCGGAACGCGTATCATCAAGCTGGAAGAGAATTACCGTTCGGTACAGC
CTGTGCTTGATCTGACCAACGCCATTCTGGACGATGCGCCGCAGGCGTTCCGCAAAAAGCTGTTCACCCGGCGCGAAGGA
GGTCCTACCCCTCATGTGCTGCGCCCGCTTTCAGACCTGACTCAGGCGTCCATGGTCACATCAAAAATAACCGAGCTGCT
GCGTACATACCCACCGGATGAAATCGCCGTGCTGTTCCGCGCCGGATACCAGTCCTACCATGTGGAGATGCAGCTGGGAA
AGCTGGGCGTCAAGTTCAAGAAGTACGGCGGGGTTCGTTATTCTGACGCCGCTCATGTCAAGGACGTTCTTTCCTACATA
CGGCTGGTGGTCAACCCGCTGGATCTGCCGGCGTTTCAGCGGATGGCTTCGCTGGTAAAGGGCATAGGGGCAAAAACCTG
CCTGAAGCTGTACGATGCGGCCCAGAAAGCGGACAGAAGTGTTTTGAAAAAGGCCTGTGCACGGTTTCCCGACCTGCAGC
ACGATCTGGACATGCTGGATGCACTGCGGGCAAAGCCGCTTACTCCGGCCGGTCTGACGCAGGCCGTTATTGAGCATTAC
CAGCCGCGGCTCGAAGCCAACTATCCTGACGACTACCCCCGCAGACAGCACGGGCTGGAACAGCTTGTCCAGATTGCGGC
ATCGTACAACGATGTGGATCTGATGCTTTCGGACCTGTGTCTGGAGGACCCCGCAACGGACAAAGGTGAAGACATCAGCG
CGGTCACGTTGTCCACCGTGCATTCCGCCAAGGGGCTGGAATGGTCGGCGGTGCTGCTCATTGATCTGGTGGAAGACCGT
TTTCCCTCGCGCCATGCCCTGAACAAGGCAGAGGACTTTGAGGAAGAGCGGCGGCTTATGTATGTGGCGTGCACGCGGGC
CCGTGATTATCTGGCGCTGTTTGTGCCTGCCACCATCTACGACCGCAGCGGAGGCGGCAATACACCGGCCAATCCCAGCC
CCTTTGTGCGTGACCTGCCCGCCCATCTTTATGAAGACTGGCGCGAAAACTTTACCGGCACGCTTACCCGCAGGCAGGAT
GCCCGTCCGGTTACTGTTTCTGCCGCGCGCACGTCCGGCCCTGCGTCCGGCGGTGGCCGGCAGGCGGAACAGGGCGGCGG
CGCGCTGGGCAACGGCGGCAGACCGGCTTCGGGGTATTGCACGCACAAGATTTTCGGCAGGGGCAAAATTATTCAGCATG
TGCCGCCCGACAAGTACCGCGTCAATTTTCCGGGATTCGGTCTCAAGGTCATTATGGCCCAGTATCTTACTCTGGAATGA

Upstream 100 bases:

>100_bases
GTGCTGGAGCATTTCGGTCTGGCCCCTGTCGCACAATCAGGCCGCCCCGCGGCGACGGGGCTTATCCACCCGCCCGGCAA
CAAGTAACGATACCATATAT

Downstream 100 bases:

>100_bases
TAACGGCCCGCTGCCGCGGCCGCCGCAAAATTCACCGGAACCCACACGGATGCGCATATGAGCACCATGACTTCCGAAGA
TGCGTTTCTTGCCTGTATTG

Product: ATP-dependent DNA helicase UvrD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 719; Mature: 719

Protein sequence:

>719_residues
MIDYKKELNAAQYEAATTLEGPMLVIAGAGSGKTRTVVYRLANMVQQGIAASNILLLTFTRKAAQEMQHRAAQLLGQSAG
MVGGVQGGTFHAFAYSILRMHKPQGYEGDLSVMDSADAAGALKHCKDTLGLGKGDRSFPKTQTVMGLLSKARNKELTVED
VLRREAFHLASYAGDLTRISDTYRAFKRRHALMDYDDLLFELERLLQSDADLLAYYRDRFRYIMVDEFQDTNLVQARLVR
LLGSGHNNVMAVGDDAQSIYAFRGANVQNILDFPDMFPGTRIIKLEENYRSVQPVLDLTNAILDDAPQAFRKKLFTRREG
GPTPHVLRPLSDLTQASMVTSKITELLRTYPPDEIAVLFRAGYQSYHVEMQLGKLGVKFKKYGGVRYSDAAHVKDVLSYI
RLVVNPLDLPAFQRMASLVKGIGAKTCLKLYDAAQKADRSVLKKACARFPDLQHDLDMLDALRAKPLTPAGLTQAVIEHY
QPRLEANYPDDYPRRQHGLEQLVQIAASYNDVDLMLSDLCLEDPATDKGEDISAVTLSTVHSAKGLEWSAVLLIDLVEDR
FPSRHALNKAEDFEEERRLMYVACTRARDYLALFVPATIYDRSGGGNTPANPSPFVRDLPAHLYEDWRENFTGTLTRRQD
ARPVTVSAARTSGPASGGGRQAEQGGGALGNGGRPASGYCTHKIFGRGKIIQHVPPDKYRVNFPGFGLKVIMAQYLTLE

Sequences:

>Translated_719_residues
MIDYKKELNAAQYEAATTLEGPMLVIAGAGSGKTRTVVYRLANMVQQGIAASNILLLTFTRKAAQEMQHRAAQLLGQSAG
MVGGVQGGTFHAFAYSILRMHKPQGYEGDLSVMDSADAAGALKHCKDTLGLGKGDRSFPKTQTVMGLLSKARNKELTVED
VLRREAFHLASYAGDLTRISDTYRAFKRRHALMDYDDLLFELERLLQSDADLLAYYRDRFRYIMVDEFQDTNLVQARLVR
LLGSGHNNVMAVGDDAQSIYAFRGANVQNILDFPDMFPGTRIIKLEENYRSVQPVLDLTNAILDDAPQAFRKKLFTRREG
GPTPHVLRPLSDLTQASMVTSKITELLRTYPPDEIAVLFRAGYQSYHVEMQLGKLGVKFKKYGGVRYSDAAHVKDVLSYI
RLVVNPLDLPAFQRMASLVKGIGAKTCLKLYDAAQKADRSVLKKACARFPDLQHDLDMLDALRAKPLTPAGLTQAVIEHY
QPRLEANYPDDYPRRQHGLEQLVQIAASYNDVDLMLSDLCLEDPATDKGEDISAVTLSTVHSAKGLEWSAVLLIDLVEDR
FPSRHALNKAEDFEEERRLMYVACTRARDYLALFVPATIYDRSGGGNTPANPSPFVRDLPAHLYEDWRENFTGTLTRRQD
ARPVTVSAARTSGPASGGGRQAEQGGGALGNGGRPASGYCTHKIFGRGKIIQHVPPDKYRVNFPGFGLKVIMAQYLTLE
>Mature_719_residues
MIDYKKELNAAQYEAATTLEGPMLVIAGAGSGKTRTVVYRLANMVQQGIAASNILLLTFTRKAAQEMQHRAAQLLGQSAG
MVGGVQGGTFHAFAYSILRMHKPQGYEGDLSVMDSADAAGALKHCKDTLGLGKGDRSFPKTQTVMGLLSKARNKELTVED
VLRREAFHLASYAGDLTRISDTYRAFKRRHALMDYDDLLFELERLLQSDADLLAYYRDRFRYIMVDEFQDTNLVQARLVR
LLGSGHNNVMAVGDDAQSIYAFRGANVQNILDFPDMFPGTRIIKLEENYRSVQPVLDLTNAILDDAPQAFRKKLFTRREG
GPTPHVLRPLSDLTQASMVTSKITELLRTYPPDEIAVLFRAGYQSYHVEMQLGKLGVKFKKYGGVRYSDAAHVKDVLSYI
RLVVNPLDLPAFQRMASLVKGIGAKTCLKLYDAAQKADRSVLKKACARFPDLQHDLDMLDALRAKPLTPAGLTQAVIEHY
QPRLEANYPDDYPRRQHGLEQLVQIAASYNDVDLMLSDLCLEDPATDKGEDISAVTLSTVHSAKGLEWSAVLLIDLVEDR
FPSRHALNKAEDFEEERRLMYVACTRARDYLALFVPATIYDRSGGGNTPANPSPFVRDLPAHLYEDWRENFTGTLTRRQD
ARPVTVSAARTSGPASGGGRQAEQGGGALGNGGRPASGYCTHKIFGRGKIIQHVPPDKYRVNFPGFGLKVIMAQYLTLE

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=757, Percent_Identity=33.0250990752972, Blast_Score=306, Evalue=3e-84,
Organism=Escherichia coli, GI48994965, Length=661, Percent_Identity=29.6520423600605, Blast_Score=232, Evalue=7e-62,
Organism=Escherichia coli, GI1789183, Length=142, Percent_Identity=32.3943661971831, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322369, Length=443, Percent_Identity=25.9593679458239, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324477, Length=398, Percent_Identity=23.3668341708543, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 79777; Mature: 79777

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDYKKELNAAQYEAATTLEGPMLVIAGAGSGKTRTVVYRLANMVQQGIAASNILLLTFT
CCCCHHHCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEC
RKAAQEMQHRAAQLLGQSAGMVGGVQGGTFHAFAYSILRMHKPQGYEGDLSVMDSADAAG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHH
ALKHCKDTLGLGKGDRSFPKTQTVMGLLSKARNKELTVEDVLRREAFHLASYAGDLTRIS
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHH
DTYRAFKRRHALMDYDDLLFELERLLQSDADLLAYYRDRFRYIMVDEFQDTNLVQARLVR
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCHHHHHHHHH
LLGSGHNNVMAVGDDAQSIYAFRGANVQNILDFPDMFPGTRIIKLEENYRSVQPVLDLTN
HHCCCCCCEEEECCCHHHHHHCCCCCHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHH
AILDDAPQAFRKKLFTRREGGPTPHVLRPLSDLTQASMVTSKITELLRTYPPDEIAVLFR
HHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AGYQSYHVEMQLGKLGVKFKKYGGVRYSDAAHVKDVLSYIRLVVNPLDLPAFQRMASLVK
HCCCCEEEEEEECHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GIGAKTCLKLYDAAQKADRSVLKKACARFPDLQHDLDMLDALRAKPLTPAGLTQAVIEHY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHC
QPRLEANYPDDYPRRQHGLEQLVQIAASYNDVDLMLSDLCLEDPATDKGEDISAVTLSTV
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEHHHH
HSAKGLEWSAVLLIDLVEDRFPSRHALNKAEDFEEERRLMYVACTRARDYLALFVPATIY
HHCCCCCHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHEEEEEECCCCCEEEEEECHHEE
DRSGGGNTPANPSPFVRDLPAHLYEDWRENFTGTLTRRQDARPVTVSAARTSGPASGGGR
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCCCCCCCCCC
QAEQGGGALGNGGRPASGYCTHKIFGRGKIIQHVPPDKYRVNFPGFGLKVIMAQYLTLE
CCHHCCCCCCCCCCCCCCCCHHHHHCCCCEEECCCCCCEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIDYKKELNAAQYEAATTLEGPMLVIAGAGSGKTRTVVYRLANMVQQGIAASNILLLTFT
CCCCHHHCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEC
RKAAQEMQHRAAQLLGQSAGMVGGVQGGTFHAFAYSILRMHKPQGYEGDLSVMDSADAAG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHH
ALKHCKDTLGLGKGDRSFPKTQTVMGLLSKARNKELTVEDVLRREAFHLASYAGDLTRIS
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHH
DTYRAFKRRHALMDYDDLLFELERLLQSDADLLAYYRDRFRYIMVDEFQDTNLVQARLVR
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCHHHHHHHHH
LLGSGHNNVMAVGDDAQSIYAFRGANVQNILDFPDMFPGTRIIKLEENYRSVQPVLDLTN
HHCCCCCCEEEECCCHHHHHHCCCCCHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHH
AILDDAPQAFRKKLFTRREGGPTPHVLRPLSDLTQASMVTSKITELLRTYPPDEIAVLFR
HHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AGYQSYHVEMQLGKLGVKFKKYGGVRYSDAAHVKDVLSYIRLVVNPLDLPAFQRMASLVK
HCCCCEEEEEEECHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GIGAKTCLKLYDAAQKADRSVLKKACARFPDLQHDLDMLDALRAKPLTPAGLTQAVIEHY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHC
QPRLEANYPDDYPRRQHGLEQLVQIAASYNDVDLMLSDLCLEDPATDKGEDISAVTLSTV
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEHHHH
HSAKGLEWSAVLLIDLVEDRFPSRHALNKAEDFEEERRLMYVACTRARDYLALFVPATIY
HHCCCCCHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHEEEEEECCCCCEEEEEECHHEE
DRSGGGNTPANPSPFVRDLPAHLYEDWRENFTGTLTRRQDARPVTVSAARTSGPASGGGR
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCCCCCCCCCC
QAEQGGGALGNGGRPASGYCTHKIFGRGKIIQHVPPDKYRVNFPGFGLKVIMAQYLTLE
CCHHCCCCCCCCCCCCCCCCHHHHHCCCCEEECCCCCCEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA