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The map label for this gene is iciA [H]

Identifier: 78358513

GI number: 78358513

Start: 3435579

End: 3436529

Strand: Reverse

Name: iciA [H]

Synonym: Dde_3474

Alternate gene names: 78358513

Gene position: 3436529-3435579 (Counterclockwise)

Preceding gene: 78358515

Following gene: 78358511

Centisome position: 92.13

GC content: 64.56

Gene sequence:

>951_bases
ATGCTTGAATACCGGCTTATAGAGGCTCTGGCCATGGTGGTGCGCGAAGGCGGGTTTGAAAAAGCAGCCCGTGTGCTGCA
TGTGACCCAGTCGGCCGTGTCACAGCGTATCCGTCAGCTGGAAGCGGCAGCGGGTACGGCGCTGGTCACACGGGCGGCAC
CTGTTACACCTACCGAGGCAGGCCGCAGACTGCTGCGGCATTATCATCAGGTTTCCATGCTTGAGGCTGACCTTGAGCGC
GACATGGCCGCGCGCTTCAGCGCGGAGCTGCTGGGCGAAGGCGGTGCTGCGCACGGGCGGGCCCCCGCCTACCGGCAGCT
GAGTGTGGCCGTCAATGCCGACAGTCTGGCCACATGGTTTCTGGAGGCGGTGGATGCCGTAGCGGAACAGGAAAAGCTGC
TGCTTGATGTGCTGGTGGATGATCAGGACAGAACGCATGACCTGCTGCGTAACGGTGAAGTGGTGGGCTGTGTGAGCACG
CGGGCCACGCCGTTTCACGGGTGCAGCATGCAGCGGCTGGGCAGTATGGAGTACCTTGTGTGCGCTGCGCCGGATTTTGC
CGCGGAACACGGCCTGCGCGTGGGGGCGGACGGCCGTGCCGCCACTGCCGCCGATCTGCTGCGGGCGCCTGCCGTGCTGT
ACAACAGGGTGGACGGGGTGCATGACACCATGCTGCAGACCTTGTACGGCATTGCGCCGGACACCCCGTATCCGCGGCAC
TATGTGCCTTCTTCGGAGGCATTTGTAAAAATGGTGGCTGCGGGGTTCGGGTGGGGCTGCGTGCCGCATCTGCAGGCCGA
ACCGCTGCTTGCCGGCGGTGAACTGGTCGATCTGGCACCGGGCTGGGCATTGCCCGTGGCGCTGCACTGGCACTGGTGGG
ATCTGGGTACGGGGCTTTTCAAGCGCATGGCCGCGGTCCTTTCGGACCATGCGGCTCTTGTTCTGCGCTGA

Upstream 100 bases:

>100_bases
GGGTCCTCTGGTGTGGTTGCGTGCATGGGAGATATACCCCCGCGTTCAAGAAGTGAAATTACTAGTTTATATGCTGCATA
AGTTTCGCTAATGTCAGGTC

Downstream 100 bases:

>100_bases
GGCGTATGTCAGCTTTTTGCCAGCGCCTCGTCAAACCATTTGGGGCTGCATACGGGCTGCCATGCTGCCATGCGGTCCAG
CAGCGCTGCGGCGTTTTGTG

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MLEYRLIEALAMVVREGGFEKAARVLHVTQSAVSQRIRQLEAAAGTALVTRAAPVTPTEAGRRLLRHYHQVSMLEADLER
DMAARFSAELLGEGGAAHGRAPAYRQLSVAVNADSLATWFLEAVDAVAEQEKLLLDVLVDDQDRTHDLLRNGEVVGCVST
RATPFHGCSMQRLGSMEYLVCAAPDFAAEHGLRVGADGRAATAADLLRAPAVLYNRVDGVHDTMLQTLYGIAPDTPYPRH
YVPSSEAFVKMVAAGFGWGCVPHLQAEPLLAGGELVDLAPGWALPVALHWHWWDLGTGLFKRMAAVLSDHAALVLR

Sequences:

>Translated_316_residues
MLEYRLIEALAMVVREGGFEKAARVLHVTQSAVSQRIRQLEAAAGTALVTRAAPVTPTEAGRRLLRHYHQVSMLEADLER
DMAARFSAELLGEGGAAHGRAPAYRQLSVAVNADSLATWFLEAVDAVAEQEKLLLDVLVDDQDRTHDLLRNGEVVGCVST
RATPFHGCSMQRLGSMEYLVCAAPDFAAEHGLRVGADGRAATAADLLRAPAVLYNRVDGVHDTMLQTLYGIAPDTPYPRH
YVPSSEAFVKMVAAGFGWGCVPHLQAEPLLAGGELVDLAPGWALPVALHWHWWDLGTGLFKRMAAVLSDHAALVLR
>Mature_316_residues
MLEYRLIEALAMVVREGGFEKAARVLHVTQSAVSQRIRQLEAAAGTALVTRAAPVTPTEAGRRLLRHYHQVSMLEADLER
DMAARFSAELLGEGGAAHGRAPAYRQLSVAVNADSLATWFLEAVDAVAEQEKLLLDVLVDDQDRTHDLLRNGEVVGCVST
RATPFHGCSMQRLGSMEYLVCAAPDFAAEHGLRVGADGRAATAADLLRAPAVLYNRVDGVHDTMLQTLYGIAPDTPYPRH
YVPSSEAFVKMVAAGFGWGCVPHLQAEPLLAGGELVDLAPGWALPVALHWHWWDLGTGLFKRMAAVLSDHAALVLR

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=314, Percent_Identity=39.171974522293, Blast_Score=201, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34229; Mature: 34229

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEYRLIEALAMVVREGGFEKAARVLHVTQSAVSQRIRQLEAAAGTALVTRAAPVTPTEA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCHHHH
GRRLLRHYHQVSMLEADLERDMAARFSAELLGEGGAAHGRAPAYRQLSVAVNADSLATWF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHH
LEAVDAVAEQEKLLLDVLVDDQDRTHDLLRNGEVVGCVSTRATPFHGCSMQRLGSMEYLV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHCCCCEEE
CAAPDFAAEHGLRVGADGRAATAADLLRAPAVLYNRVDGVHDTMLQTLYGIAPDTPYPRH
EECCCHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
YVPSSEAFVKMVAAGFGWGCVPHLQAEPLLAGGELVDLAPGWALPVALHWHWWDLGTGLF
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEEHHHHHHHHH
KRMAAVLSDHAALVLR
HHHHHHHCCCCCEEEC
>Mature Secondary Structure
MLEYRLIEALAMVVREGGFEKAARVLHVTQSAVSQRIRQLEAAAGTALVTRAAPVTPTEA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCHHHH
GRRLLRHYHQVSMLEADLERDMAARFSAELLGEGGAAHGRAPAYRQLSVAVNADSLATWF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHH
LEAVDAVAEQEKLLLDVLVDDQDRTHDLLRNGEVVGCVSTRATPFHGCSMQRLGSMEYLV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHCCCCEEE
CAAPDFAAEHGLRVGADGRAATAADLLRAPAVLYNRVDGVHDTMLQTLYGIAPDTPYPRH
EECCCHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
YVPSSEAFVKMVAAGFGWGCVPHLQAEPLLAGGELVDLAPGWALPVALHWHWWDLGTGLF
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEEHHHHHHHHH
KRMAAVLSDHAALVLR
HHHHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]