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The map label for this gene is adhE [H]

Identifier: 78358323

GI number: 78358323

Start: 3274261

End: 3275766

Strand: Reverse

Name: adhE [H]

Synonym: Dde_3283

Alternate gene names: 78358323

Gene position: 3275766-3274261 (Counterclockwise)

Preceding gene: 78358324

Following gene: 78358322

Centisome position: 87.82

GC content: 60.23

Gene sequence:

>1506_bases
ATGATTGTCGATAACGATCTGATTTCCATACAGGAAGCCAGAATTCTGGCAGAAAACGCAGGGCGCGCACAGCGGGTTCT
GGCCACGTTTTCTCAGGAACAGCTGGACGAGATCGTCGAAGCCGTAGCCGAAGCCGTGGGGGCGCAGGCGCAAGCGCTTG
CGGACATGGCGTACGAAGAAACCGACTACGGCAACCCGCATGACAAGGCAGTGAAAAACCGCTTTGTATGCACGCAGGTG
CGCGAGCAGCTGCGCGGCATGCGGTGTGTCGGCGTCATCGGACGGGATGAGCACGCACGCACCATGGACATTGGTGTACC
CGTAGGCGTCATTGCCGCGTTGTGTCCGGCGACCAGCCCCGTGTCCACAACCATCTGCAAGACGCTCATAGCCGTCAAAT
CAGGCAACGCCGTAGTTTTTTCCCCTCATCCGCGCGCTGCCAGAAGCATAGCCGCCGCGCTTGATATCATGATTGATGCC
GCGCGGGCTCACGGTCTGCCGGAAGGATGTCTTTCATACATGTCCACGGTAACCAAAAGCGGTACCGCCGAACTGATGAA
CCATCCTGCCACATCGCTGGTCATGCTTTCGGGCGTGCCCGGCATGATAGGGCTTGCGCGCTGCAGCGGCAAACCCGTGA
TTTACGGCGGCACCGGCAACGGGCCTGCATTTGTCGAACGCTCGGCCGACATCCGCCGCGCCGTGGCCGACATCATGCTC
AGCAAGACGTTCGACAACGGTATCGCGCCGTCCGCGGAACAGTCCATCGTGGTCGATTCCTGCATCGAGCGGGAAGTCAG
ACATGCCCTGCAGGAGTGCGGCGCATACTTCATGACGGACGAGGAGTCCCTGCGGCTGGCGGAGCTTCTTTTCTGTGCCG
ACGGACGCCACAAAGCCGGCATGGTTGGTGTGTCTGCCCCGGTTCTGGCGCGCAGGGCAGGCATCAGCGTGCCCGACAAC
GTGCGGCTGCTGGTGGCAGAACGTAAATATGTTTCCGAAGCAGACCCGTATTCAAGGGAACTGCTTTCCCCCGTCCTTGC
CTATTACGTGGAGGACGACTGGATGCACGCCTGTGAAAAGTGCATCGAGCTGCTGCTCCACGAACGCAACGCTCATACTC
TCGTGATCCATTCCAGTAACGAAGAGGTGATTTTGCAGTTTGCCCTGAAAAAACCTGTGGGCCGTCTGCTTGTAAACACT
CCCGCCACATTCGGCGGCATGGGTGTGACCACCAATCTCTTCCCGTCCATGACGCTGGGCAGCGGGTCCGCAGGGCGCGG
GATCACCGCCGACAACGTGTCGCCGATGAATCTCATCTACATACGCAAGGTCGGTTACGGCGTGCGGCAGTACACCGGCG
CACAGGACGCCGCGGGATACGGTTCCCGCGTTTGCGCCGCGCAGGGCGTCATGGCCCCTCATGACAGCGATTCCAGAAAA
ATGCAGGCGTTGCACCGGATCCTGATGGAAGCGATCGGGGTCATGGACGGGGCAGCACACAGATGA

Upstream 100 bases:

>100_bases
GAATCTGCTGCCCTAGTGGTGACGGACAACGGGCAGCAGTGCGCACGGGGCGTTCTGAATGGTCGCCGCAAGGACACAGG
AAACGGCAAAGGGGTAGATG

Downstream 100 bases:

>100_bases
AGGAACTTATCCGTTAAAGGGAGGATGCAAAGTGGATCTCCAGGACTTTTCACATAAGCTCGCGGAAGCGACAAAGAACC
TTACTCCGGCAGAACGTGCT

Product: acetaldehyde dehydrogenase-like

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MIVDNDLISIQEARILAENAGRAQRVLATFSQEQLDEIVEAVAEAVGAQAQALADMAYEETDYGNPHDKAVKNRFVCTQV
REQLRGMRCVGVIGRDEHARTMDIGVPVGVIAALCPATSPVSTTICKTLIAVKSGNAVVFSPHPRAARSIAAALDIMIDA
ARAHGLPEGCLSYMSTVTKSGTAELMNHPATSLVMLSGVPGMIGLARCSGKPVIYGGTGNGPAFVERSADIRRAVADIML
SKTFDNGIAPSAEQSIVVDSCIEREVRHALQECGAYFMTDEESLRLAELLFCADGRHKAGMVGVSAPVLARRAGISVPDN
VRLLVAERKYVSEADPYSRELLSPVLAYYVEDDWMHACEKCIELLLHERNAHTLVIHSSNEEVILQFALKKPVGRLLVNT
PATFGGMGVTTNLFPSMTLGSGSAGRGITADNVSPMNLIYIRKVGYGVRQYTGAQDAAGYGSRVCAAQGVMAPHDSDSRK
MQALHRILMEAIGVMDGAAHR

Sequences:

>Translated_501_residues
MIVDNDLISIQEARILAENAGRAQRVLATFSQEQLDEIVEAVAEAVGAQAQALADMAYEETDYGNPHDKAVKNRFVCTQV
REQLRGMRCVGVIGRDEHARTMDIGVPVGVIAALCPATSPVSTTICKTLIAVKSGNAVVFSPHPRAARSIAAALDIMIDA
ARAHGLPEGCLSYMSTVTKSGTAELMNHPATSLVMLSGVPGMIGLARCSGKPVIYGGTGNGPAFVERSADIRRAVADIML
SKTFDNGIAPSAEQSIVVDSCIEREVRHALQECGAYFMTDEESLRLAELLFCADGRHKAGMVGVSAPVLARRAGISVPDN
VRLLVAERKYVSEADPYSRELLSPVLAYYVEDDWMHACEKCIELLLHERNAHTLVIHSSNEEVILQFALKKPVGRLLVNT
PATFGGMGVTTNLFPSMTLGSGSAGRGITADNVSPMNLIYIRKVGYGVRQYTGAQDAAGYGSRVCAAQGVMAPHDSDSRK
MQALHRILMEAIGVMDGAAHR
>Mature_501_residues
MIVDNDLISIQEARILAENAGRAQRVLATFSQEQLDEIVEAVAEAVGAQAQALADMAYEETDYGNPHDKAVKNRFVCTQV
REQLRGMRCVGVIGRDEHARTMDIGVPVGVIAALCPATSPVSTTICKTLIAVKSGNAVVFSPHPRAARSIAAALDIMIDA
ARAHGLPEGCLSYMSTVTKSGTAELMNHPATSLVMLSGVPGMIGLARCSGKPVIYGGTGNGPAFVERSADIRRAVADIML
SKTFDNGIAPSAEQSIVVDSCIEREVRHALQECGAYFMTDEESLRLAELLFCADGRHKAGMVGVSAPVLARRAGISVPDN
VRLLVAERKYVSEADPYSRELLSPVLAYYVEDDWMHACEKCIELLLHERNAHTLVIHSSNEEVILQFALKKPVGRLLVNT
PATFGGMGVTTNLFPSMTLGSGSAGRGITADNVSPMNLIYIRKVGYGVRQYTGAQDAAGYGSRVCAAQGVMAPHDSDSRK
MQALHRILMEAIGVMDGAAHR

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787493, Length=443, Percent_Identity=37.020316027088, Blast_Score=278, Evalue=7e-76,
Organism=Escherichia coli, GI1788797, Length=379, Percent_Identity=28.4960422163588, Blast_Score=118, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 53620; Mature: 53620

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVDNDLISIQEARILAENAGRAQRVLATFSQEQLDEIVEAVAEAVGAQAQALADMAYEE
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHC
TDYGNPHDKAVKNRFVCTQVREQLRGMRCVGVIGRDEHARTMDIGVPVGVIAALCPATSP
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCHHHHHHHCCCCCC
VSTTICKTLIAVKSGNAVVFSPHPRAARSIAAALDIMIDAARAHGLPEGCLSYMSTVTKS
HHHHHHHHHHHEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
GTAELMNHPATSLVMLSGVPGMIGLARCSGKPVIYGGTGNGPAFVERSADIRRAVADIML
CHHHHHCCCCHHEEEECCCCCHHHHHCCCCCCEEECCCCCCCHHEECHHHHHHHHHHHHH
SKTFDNGIAPSAEQSIVVDSCIEREVRHALQECGAYFMTDEESLRLAELLFCADGRHKAG
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCCCC
MVGVSAPVLARRAGISVPDNVRLLVAERKYVSEADPYSRELLSPVLAYYVEDDWMHACEK
EEECCHHHHHHHCCCCCCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHH
CIELLLHERNAHTLVIHSSNEEVILQFALKKPVGRLLVNTPATFGGMGVTTNLFPSMTLG
HHHHHHHCCCCCEEEEECCCCCEEEHHHHHCCHHHHEECCCCCCCCCCCCCCCCCCCCCC
SGSAGRGITADNVSPMNLIYIRKVGYGVRQYTGAQDAAGYGSRVCAAQGVMAPHDSDSRK
CCCCCCCCCCCCCCCCEEEEEEECCCCHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCHHH
MQALHRILMEAIGVMDGAAHR
HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIVDNDLISIQEARILAENAGRAQRVLATFSQEQLDEIVEAVAEAVGAQAQALADMAYEE
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHC
TDYGNPHDKAVKNRFVCTQVREQLRGMRCVGVIGRDEHARTMDIGVPVGVIAALCPATSP
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCHHHHHHHCCCCCC
VSTTICKTLIAVKSGNAVVFSPHPRAARSIAAALDIMIDAARAHGLPEGCLSYMSTVTKS
HHHHHHHHHHHEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
GTAELMNHPATSLVMLSGVPGMIGLARCSGKPVIYGGTGNGPAFVERSADIRRAVADIML
CHHHHHCCCCHHEEEECCCCCHHHHHCCCCCCEEECCCCCCCHHEECHHHHHHHHHHHHH
SKTFDNGIAPSAEQSIVVDSCIEREVRHALQECGAYFMTDEESLRLAELLFCADGRHKAG
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCCCC
MVGVSAPVLARRAGISVPDNVRLLVAERKYVSEADPYSRELLSPVLAYYVEDDWMHACEK
EEECCHHHHHHHCCCCCCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHH
CIELLLHERNAHTLVIHSSNEEVILQFALKKPVGRLLVNTPATFGGMGVTTNLFPSMTLG
HHHHHHHCCCCCEEEEECCCCCEEEHHHHHCCHHHHEECCCCCCCCCCCCCCCCCCCCCC
SGSAGRGITADNVSPMNLIYIRKVGYGVRQYTGAQDAAGYGSRVCAAQGVMAPHDSDSRK
CCCCCCCCCCCCCCCCEEEEEEECCCCHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCHHH
MQALHRILMEAIGVMDGAAHR
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]