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The map label for this gene is anfA [H]

Identifier: 78355867

GI number: 78355867

Start: 841231

End: 842778

Strand: Direct

Name: anfA [H]

Synonym: Dde_0820

Alternate gene names: 78355867

Gene position: 841231-842778 (Clockwise)

Preceding gene: 78355866

Following gene: 78355869

Centisome position: 22.55

GC content: 59.3

Gene sequence:

>1548_bases
ATGGCCAACGTCATGATTGTGGACAGGCAATCCGATTTTATACGGAATATGGAGCGGACGCTTCTTTCCGAAGGGCACAC
GTTGTCCATCGCCGGTTCGGTGACGGAGTGCCTTGACAGGTGCCGGTTCCGGCCCTGTGATCTTGTCATACTTGAAGACC
GTCTGGCCGCGGAATGTCATAACAGCGTCGCCTCGTTCCGGCGGCTGGCTCCCGCGCCGGAAGTTGTGGTGGTTACAGGG
CGCGGTGACCCGCTGCTGGGCGAAATAGCCCTGCTGGAAGGCGCGCTCAATTATCTTGAAAAGCAGAATGCCCCCGCAGC
AATCCGTTCTTTGCTCACGCGGCTGGGGCAGAACAGCATTCTTTCCGGAAACGGCGCAGGCACTGCGGCGCGTTTCGGCA
TCGTGGGCGAATCTGCCCGCATTATTTCATGTTACAATCTTATCATGCGCTCGCGCGCGTCGGATGTGAGCGTATTGCTG
GAAGGCGAGACAGGTACCGGCAAGGAGCTGTTCGCCAAGGCCATCCACAAACTGAGCCCCCGCGTTGACGGGCAGCTGGT
GACCGTGGACTGCGCTTCGCTGCCTGAAACGCTGGTTGAAAGTCTGCTTTTCGGGTATTCGCGCGGAGCGTTTACCGGTG
CCGACGGCAATCGGGTGGGGCTGGTGAAGCGGGCTGACGGCGGCACTCTTTTTCTGGATGAAGTGGGCGAACTGCCGCTG
AGCGTGCAGAAAAAGTTTTTGCGGGTGCTGCAGGAACGCAGGTTCCGTCCTGTGGGGGGCGGAAATGAAATTTCAAGCGA
TTTCAGACTGATTGCAGCGACAAACCGTAATCTTCAGGAAATGGTTGATCAGGGGCTTTTCCGCAGTGACCTTTTGTACA
GGCTGCGCACGCTGAAGATAACCTTGCCCCCGTTGCGTGACCGCGGCGAAGACGTTGTGCTTATCGCCGGGCATGTCATC
AAGGAACAGTGCCGCCGGGCCGGAATACCATGCAAACGTCTTTCCGGTGAATTTCTTGAAAAATTGAGGGGCTACCACTG
GCCTGGCAATGTGCGCGAACTGGTCAACATGGTCGAAAGCGCTTTTGCCATGGCGTGGGACGACGAGGTATTGCATACGG
AGCACCTGCCCCCGCAGCTCCATGCGCGGCTTGTGCGCTCCTATTACAGCAGCCGCGGGCTGGCGCAGGGTGACAACTCG
TGCAGTATGTCCCCGGCGGCGCAGGCCGTAACCGTACAGGTGCCAGTGCGTCCGGTGGCAGAGCCTGCCGTTTCCGCCGA
CAGACCCCCTGCAGACGTTTCCGGCTGTTCCGGCGGGCACGGCTGCAATGAAACTTCTTTGCAGCACAGCCCGGCATCAT
TCGAAGAGTTTGAGGCCATGGGGTCGCTGCGCGACGCGCGGCGTCTGGTCACAGAGCGTTTTGAACGCGATTACCTGCGG
GCGCTGAGAGCGGCCACAGGGCATGATGTAAAAAAAGCCTGTGCGGTGTCGGGGCTTTCGCGTGCCCGGCTGTATGAGCT
TTACCGAAAACACGGGATACTGGGGTGA

Upstream 100 bases:

>100_bases
GAATCAAGCCAACCCGCCTCAGGCGGGACGGAAAGCCACGGGTCTTTCAGACAGCCGGGTTGCCTCGGTTCATGCCGCTG
CCGTTTCTCAGGGGGAAACT

Downstream 100 bases:

>100_bases
ATGATGCGCCGCGCAGACCGCGCGGCGCTGACTGTGGCGTGTGCGATGTATTGCAGAGGCGGTCCGGCGACTGCCTTTTT
TTTGTCTTGCTGCCGGCAAC

Product: two component Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MANVMIVDRQSDFIRNMERTLLSEGHTLSIAGSVTECLDRCRFRPCDLVILEDRLAAECHNSVASFRRLAPAPEVVVVTG
RGDPLLGEIALLEGALNYLEKQNAPAAIRSLLTRLGQNSILSGNGAGTAARFGIVGESARIISCYNLIMRSRASDVSVLL
EGETGTGKELFAKAIHKLSPRVDGQLVTVDCASLPETLVESLLFGYSRGAFTGADGNRVGLVKRADGGTLFLDEVGELPL
SVQKKFLRVLQERRFRPVGGGNEISSDFRLIAATNRNLQEMVDQGLFRSDLLYRLRTLKITLPPLRDRGEDVVLIAGHVI
KEQCRRAGIPCKRLSGEFLEKLRGYHWPGNVRELVNMVESAFAMAWDDEVLHTEHLPPQLHARLVRSYYSSRGLAQGDNS
CSMSPAAQAVTVQVPVRPVAEPAVSADRPPADVSGCSGGHGCNETSLQHSPASFEEFEAMGSLRDARRLVTERFERDYLR
ALRAATGHDVKKACAVSGLSRARLYELYRKHGILG

Sequences:

>Translated_515_residues
MANVMIVDRQSDFIRNMERTLLSEGHTLSIAGSVTECLDRCRFRPCDLVILEDRLAAECHNSVASFRRLAPAPEVVVVTG
RGDPLLGEIALLEGALNYLEKQNAPAAIRSLLTRLGQNSILSGNGAGTAARFGIVGESARIISCYNLIMRSRASDVSVLL
EGETGTGKELFAKAIHKLSPRVDGQLVTVDCASLPETLVESLLFGYSRGAFTGADGNRVGLVKRADGGTLFLDEVGELPL
SVQKKFLRVLQERRFRPVGGGNEISSDFRLIAATNRNLQEMVDQGLFRSDLLYRLRTLKITLPPLRDRGEDVVLIAGHVI
KEQCRRAGIPCKRLSGEFLEKLRGYHWPGNVRELVNMVESAFAMAWDDEVLHTEHLPPQLHARLVRSYYSSRGLAQGDNS
CSMSPAAQAVTVQVPVRPVAEPAVSADRPPADVSGCSGGHGCNETSLQHSPASFEEFEAMGSLRDARRLVTERFERDYLR
ALRAATGHDVKKACAVSGLSRARLYELYRKHGILG
>Mature_514_residues
ANVMIVDRQSDFIRNMERTLLSEGHTLSIAGSVTECLDRCRFRPCDLVILEDRLAAECHNSVASFRRLAPAPEVVVVTGR
GDPLLGEIALLEGALNYLEKQNAPAAIRSLLTRLGQNSILSGNGAGTAARFGIVGESARIISCYNLIMRSRASDVSVLLE
GETGTGKELFAKAIHKLSPRVDGQLVTVDCASLPETLVESLLFGYSRGAFTGADGNRVGLVKRADGGTLFLDEVGELPLS
VQKKFLRVLQERRFRPVGGGNEISSDFRLIAATNRNLQEMVDQGLFRSDLLYRLRTLKITLPPLRDRGEDVVLIAGHVIK
EQCRRAGIPCKRLSGEFLEKLRGYHWPGNVRELVNMVESAFAMAWDDEVLHTEHLPPQLHARLVRSYYSSRGLAQGDNSC
SMSPAAQAVTVQVPVRPVAEPAVSADRPPADVSGCSGGHGCNETSLQHSPASFEEFEAMGSLRDARRLVTERFERDYLRA
LRAATGHDVKKACAVSGLSRARLYELYRKHGILG

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=367, Percent_Identity=38.9645776566758, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1788905, Length=364, Percent_Identity=36.5384615384615, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1788550, Length=227, Percent_Identity=49.7797356828194, Blast_Score=229, Evalue=5e-61,
Organism=Escherichia coli, GI87082117, Length=212, Percent_Identity=51.8867924528302, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1790299, Length=385, Percent_Identity=35.8441558441558, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1789233, Length=248, Percent_Identity=44.758064516129, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1789087, Length=326, Percent_Identity=42.638036809816, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI87082152, Length=230, Percent_Identity=50, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI1786524, Length=272, Percent_Identity=41.5441176470588, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI87081872, Length=231, Percent_Identity=42.8571428571429, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1787583, Length=326, Percent_Identity=34.3558282208589, Blast_Score=169, Evalue=6e-43,
Organism=Escherichia coli, GI1789828, Length=167, Percent_Identity=44.311377245509, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI87081858, Length=227, Percent_Identity=29.9559471365639, Blast_Score=99, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 56371; Mature: 56240

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVMIVDRQSDFIRNMERTLLSEGHTLSIAGSVTECLDRCRFRPCDLVILEDRLAAECH
CCCEEEEECCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCCEEEECHHHHHHHH
NSVASFRRLAPAPEVVVVTGRGDPLLGEIALLEGALNYLEKQNAPAAIRSLLTRLGQNSI
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCE
LSGNGAGTAARFGIVGESARIISCYNLIMRSRASDVSVLLEGETGTGKELFAKAIHKLSP
ECCCCCCCHHEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCC
RVDGQLVTVDCASLPETLVESLLFGYSRGAFTGADGNRVGLVKRADGGTLFLDEVGELPL
CCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEECCCCEEEHHHHCCCCH
SVQKKFLRVLQERRFRPVGGGNEISSDFRLIAATNRNLQEMVDQGLFRSDLLYRLRTLKI
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHHHEE
TLPPLRDRGEDVVLIAGHVIKEQCRRAGIPCKRLSGEFLEKLRGYHWPGNVRELVNMVES
ECCCCCCCCCCEEEECCHHHHHHHHHCCCCHHHCCHHHHHHHCCCCCCCCHHHHHHHHHH
AFAMAWDDEVLHTEHLPPQLHARLVRSYYSSRGLAQGDNSCSMSPAAQAVTVQVPVRPVA
HHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCC
EPAVSADRPPADVSGCSGGHGCNETSLQHSPASFEEFEAMGSLRDARRLVTERFERDYLR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRAATGHDVKKACAVSGLSRARLYELYRKHGILG
HHHHHCCCHHHHHHHHHCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ANVMIVDRQSDFIRNMERTLLSEGHTLSIAGSVTECLDRCRFRPCDLVILEDRLAAECH
CCEEEEECCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCCEEEECHHHHHHHH
NSVASFRRLAPAPEVVVVTGRGDPLLGEIALLEGALNYLEKQNAPAAIRSLLTRLGQNSI
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCE
LSGNGAGTAARFGIVGESARIISCYNLIMRSRASDVSVLLEGETGTGKELFAKAIHKLSP
ECCCCCCCHHEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCC
RVDGQLVTVDCASLPETLVESLLFGYSRGAFTGADGNRVGLVKRADGGTLFLDEVGELPL
CCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEECCCCEEEHHHHCCCCH
SVQKKFLRVLQERRFRPVGGGNEISSDFRLIAATNRNLQEMVDQGLFRSDLLYRLRTLKI
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHHHEE
TLPPLRDRGEDVVLIAGHVIKEQCRRAGIPCKRLSGEFLEKLRGYHWPGNVRELVNMVES
ECCCCCCCCCCEEEECCHHHHHHHHHCCCCHHHCCHHHHHHHCCCCCCCCHHHHHHHHHH
AFAMAWDDEVLHTEHLPPQLHARLVRSYYSSRGLAQGDNSCSMSPAAQAVTVQVPVRPVA
HHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCC
EPAVSADRPPADVSGCSGGHGCNETSLQHSPASFEEFEAMGSLRDARRLVTERFERDYLR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRAATGHDVKKACAVSGLSRARLYELYRKHGILG
HHHHHCCCHHHHHHHHHCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]