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The map label for this gene is trxB [C]

Identifier: 78355512

GI number: 78355512

Start: 475154

End: 476071

Strand: Direct

Name: trxB [C]

Synonym: Dde_0465

Alternate gene names: 78355512

Gene position: 475154-476071 (Clockwise)

Preceding gene: 78355511

Following gene: 78355513

Centisome position: 12.74

GC content: 65.25

Gene sequence:

>918_bases
ATGGAAACCAGACAGGTTGATGTGCTTATACTGGGCGGCGGGGTGGCCGGCATGACCGCCGCCGTTTACGCCGCCCGCGC
GGGGCTGCGTGTGACGCTGCTGGAGGAGAATGCCTGCGGCGGGCTGGTAAACTGGACGCGTGTTGTCGAAAATATGCCGT
CCTACATCAGCATACCCGGCATGGAACTGGCCGACCGCATGGCGGAACAGGTGGCCGCGCTGGGGGTGGATGTGGAAGAG
GCCGCCTGCATTGAAGAAATATCACTCGCGGCCGGAGACAAAACTGTCGTGGCCGACGATGTGCGGTATGAGGCCCGTGC
CGTCATTGCCGCCACGGGGCGCAGGCCGGTGCTGCTGGACTGTGCCGGCGACTGCTGTCAGGTGCATTACTGTGCCATAT
GCGACGGCAGCGCGTACAAGGGCCGGCACGTGCTGGTGGTGGGCGGCGGCAACAGCGGGTTTGACGAGGCTCTGGCCCTG
CTGGACACGGGTGTGGCACGGGTGACTCTGATCGAGGCGCAGGACAGGTTTTTTGCCGCGGCTGCGGCGCAGCGTGCGCT
GGCCGCCCGTGAGGGTGCAGTCATGTATACCTCCACGCGGGTGACGCAGCTGCACGGGACGGATGCTCTTGAGTCCGTCA
CGCTGACCCGTGCAGACGGTTCGGAACAGCAGCTGCCGTGCGACGGAATCTTTGTGTTCATGGGGCAGGAGCCAAGCACG
CAGCTTTTCCGCGGGCAGCTGGAACTGGACGCACAAGGCTACATAGTCACTGACGGCCTGATGGCCACATCGGTGCCCGG
CGTGTTCGCGGCGGGTGACGTGCGGCCCAAGCGTTACCGGCAGATAACCACGGCCCTGTCTGACGGCACCGTGGCCGCGC
TGGAGGCGGAAAGGTATATCCGCCGTACGGCGGGCTGA

Upstream 100 bases:

>100_bases
CGGGCCGTGTATACCCGCACCGGTCTTATGAATCCTAAGGAACTGACAACACTGTATCATTCAGTATAGCGCCGCGGCGC
GGGGAGTACGGCTGAGCACC

Downstream 100 bases:

>100_bases
TGCCGGTTTTTTACTGCGGTCGCCGGGCAGGGGCATGATGCGCGGCGGCTCCGGATTTTCGCGTCCTTTCCGGCTGACGC
TGTATGACCCAGACCCGCGC

Product: thioredoxin reductase

Products: thioredoxin disulfide; NADPH; H+

Alternate protein names: ORF A [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
METRQVDVLILGGGVAGMTAAVYAARAGLRVTLLEENACGGLVNWTRVVENMPSYISIPGMELADRMAEQVAALGVDVEE
AACIEEISLAAGDKTVVADDVRYEARAVIAATGRRPVLLDCAGDCCQVHYCAICDGSAYKGRHVLVVGGGNSGFDEALAL
LDTGVARVTLIEAQDRFFAAAAAQRALAAREGAVMYTSTRVTQLHGTDALESVTLTRADGSEQQLPCDGIFVFMGQEPST
QLFRGQLELDAQGYIVTDGLMATSVPGVFAAGDVRPKRYRQITTALSDGTVAALEAERYIRRTAG

Sequences:

>Translated_305_residues
METRQVDVLILGGGVAGMTAAVYAARAGLRVTLLEENACGGLVNWTRVVENMPSYISIPGMELADRMAEQVAALGVDVEE
AACIEEISLAAGDKTVVADDVRYEARAVIAATGRRPVLLDCAGDCCQVHYCAICDGSAYKGRHVLVVGGGNSGFDEALAL
LDTGVARVTLIEAQDRFFAAAAAQRALAAREGAVMYTSTRVTQLHGTDALESVTLTRADGSEQQLPCDGIFVFMGQEPST
QLFRGQLELDAQGYIVTDGLMATSVPGVFAAGDVRPKRYRQITTALSDGTVAALEAERYIRRTAG
>Mature_305_residues
METRQVDVLILGGGVAGMTAAVYAARAGLRVTLLEENACGGLVNWTRVVENMPSYISIPGMELADRMAEQVAALGVDVEE
AACIEEISLAAGDKTVVADDVRYEARAVIAATGRRPVLLDCAGDCCQVHYCAICDGSAYKGRHVLVVGGGNSGFDEALAL
LDTGVARVTLIEAQDRFFAAAAAQRALAAREGAVMYTSTRVTQLHGTDALESVTLTRADGSEQQLPCDGIFVFMGQEPST
QLFRGQLELDAQGYIVTDGLMATSVPGVFAAGDVRPKRYRQITTALSDGTVAALEAERYIRRTAG

Specific function: Unknown

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=284, Percent_Identity=28.5211267605634, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI226437568, Length=284, Percent_Identity=28.5211267605634, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI21389617, Length=284, Percent_Identity=28.5211267605634, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1787114, Length=314, Percent_Identity=35.3503184713376, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI87081763, Length=311, Percent_Identity=34.4051446945338, Blast_Score=121, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6321898, Length=311, Percent_Identity=32.7974276527331, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320560, Length=311, Percent_Identity=32.1543408360129, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9

Molecular weight: Translated: 32214; Mature: 32214

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00573 PYRIDINE_REDOX_2 ; PS00141 ASP_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METRQVDVLILGGGVAGMTAAVYAARAGLRVTLLEENACGGLVNWTRVVENMPSYISIPG
CCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCEEECCC
MELADRMAEQVAALGVDVEEAACIEEISLAAGDKTVVADDVRYEARAVIAATGRRPVLLD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEEECCCCCEEEE
CAGDCCQVHYCAICDGSAYKGRHVLVVGGGNSGFDEALALLDTGVARVTLIEAQDRFFAA
CCCCCEEEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCHHHHH
AAAQRALAAREGAVMYTSTRVTQLHGTDALESVTLTRADGSEQQLPCDGIFVFMGQEPST
HHHHHHHHHHCCEEEEEEEEEEEECCCCCHHEEEEEECCCCCCCCCCCEEEEEECCCCCH
QLFRGQLELDAQGYIVTDGLMATSVPGVFAAGDVRPKRYRQITTALSDGTVAALEAERYI
HHEECEEEECCCCEEEECCHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEHHHHHH
RRTAG
HHCCC
>Mature Secondary Structure
METRQVDVLILGGGVAGMTAAVYAARAGLRVTLLEENACGGLVNWTRVVENMPSYISIPG
CCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCEEECCC
MELADRMAEQVAALGVDVEEAACIEEISLAAGDKTVVADDVRYEARAVIAATGRRPVLLD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEEECCCCCEEEE
CAGDCCQVHYCAICDGSAYKGRHVLVVGGGNSGFDEALALLDTGVARVTLIEAQDRFFAA
CCCCCEEEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCHHHHH
AAAQRALAAREGAVMYTSTRVTQLHGTDALESVTLTRADGSEQQLPCDGIFVFMGQEPST
HHHHHHHHHHCCEEEEEEEEEEEECCCCCHHEEEEEECCCCCCCCCCCEEEEEECCCCCH
QLFRGQLELDAQGYIVTDGLMATSVPGVFAAGDVRPKRYRQITTALSDGTVAALEAERYI
HHEECEEEECCCCEEEECCHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEHHHHHH
RRTAG
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.008 {NADPH}} 0.125 {thioredoxin} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: thioredoxin; NADP+

Specific reaction: thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1637309 [H]