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The map label for this gene is waaA [H]

Identifier: 78355414

GI number: 78355414

Start: 357981

End: 359258

Strand: Direct

Name: waaA [H]

Synonym: Dde_0367

Alternate gene names: 78355414

Gene position: 357981-359258 (Clockwise)

Preceding gene: 78355413

Following gene: 78355416

Centisome position: 9.6

GC content: 65.41

Gene sequence:

>1278_bases
ATGACCCTGAGCGCAAGACACCGTCTGCTGCTTTCGCTGTATGGCGCGGCATGGCGGTTGTGCCGCCCCCTGCTGGCACG
TCACCGCCGGCTGCGGCACGGTTTTGACGAGCGGCTGGCACCCCGCGGGCGCACCCCGCAGGCAGATCTGTGGATCCAGT
CGGCATCGGTCGGTGAATCGTATCTTGCGTGGCAGCTGCTGCGCCATCTGCCGCAGGGCAGTGCAGGCAGCGTGCTGCTG
ACCACATGCACGGTGCAGGGGCGCGGTATTCTTGATTCTGCCGCGGCCTGGTGTGCGGAGCACCGGCCGGACATGCGTGT
GGAAGTGCGCTTTTTTCCTTTAGACGAACCCGCCGTGATGCGCCGCGCGCTGCAGCTTACGGGCGCCCATGCCGTGGTGC
TGCTTGAAACGGAGCTGTGGCCTGCGCTGATGGCGGCCTGTGCGCAGGCCGGTGTACCCTATGTTGTTGTCAACGGACGC
ATGACGCCGGGAAGTCTTGCGGGCTATCTCAATTTTGACGGATTTCTGCGCGCGGTGCCTCCGGCTGCCGTGCTGGCCAT
GTCGGAGCACGATGCGGCCCGTTATGCGGTACTGTTCGGCCACGGGCGGGTGCGCGTGATGCCCAACATCAAGTTCGACA
GAGTTCCGCAGGGCGCAGCGCAGGGACCCAACCCGCTGCTGGGTACGGTGTTCAGGCCGGGAACATCAGTTGCCGTGCTG
GCTTCTGTGCGCCGCGAGGAAGAAGAGGATGCGGAATATCTGCTGCGGGCGGTGGCCGGAGAACGTCCGCGCACCTGCAT
CGCGCTGGTGCCGCGCCATCTGGAGCGGGTGGCCCGCTGGCGCATCCGGCTTGACGAGGCGGGGATGCCGTGGGTGCTGC
GTTCTGCCGTGCGTGAACCCGTGGCGCCCGGTACGGTGGTTCTGTGGGATGCTTTCGGCGAACTGGACCATGTGTACGCA
CTGGCCCGCGGTGTCTTTGTGGGCGGCAGTCTGCGCCCGCTGGGCGGACAGAATTTTCTTGAACCGCTGGCACACGGCAT
CGTGCCTGTTATCGGGCCGCACTGGAGTAATTTTTACTGGGTGGGCGACGATATAATGGAGCAGGGGCTTGTGCAGCGTA
CCGGCGGGCCGGAGGAGGCAGCGCAGGAACTGCTGCGTCTGCTCAGACGTCCGGCCCCCAAGACAAGAGTGGCGGCACGG
TTTGCCGGATATGTTGAAAAGCGCCGCGGCGGTGCCCGCATGGCCGCTGAAACCGTGGCGGAAATTATCCGCAGCTGA

Upstream 100 bases:

>100_bases
TGCCGCAGGAAGCGGCTGCCGTGGGCATTTCCTTTGACGGGTTGATCGGGCGGCTTATCGAGCTGGGACTGGCCGCCCAC
GGCAAACAACAGGAGAAGGC

Downstream 100 bases:

>100_bases
CCTGCATTTTCGGTTTACGCACAGCTTCAGGCTGCCGCCGGTGTTATCTCCGGTTGGCAGCTTTTTTAGTGTCTGCGGGG
TGCCGCAGCCGCGCGCATGG

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTLSARHRLLLSLYGAAWRLCRPLLARHRRLRHGFDERLAPRGRTPQADLWIQSASVGESYLAWQLLRHLPQGSAGSVLL
TTCTVQGRGILDSAAAWCAEHRPDMRVEVRFFPLDEPAVMRRALQLTGAHAVVLLETELWPALMAACAQAGVPYVVVNGR
MTPGSLAGYLNFDGFLRAVPPAAVLAMSEHDAARYAVLFGHGRVRVMPNIKFDRVPQGAAQGPNPLLGTVFRPGTSVAVL
ASVRREEEEDAEYLLRAVAGERPRTCIALVPRHLERVARWRIRLDEAGMPWVLRSAVREPVAPGTVVLWDAFGELDHVYA
LARGVFVGGSLRPLGGQNFLEPLAHGIVPVIGPHWSNFYWVGDDIMEQGLVQRTGGPEEAAQELLRLLRRPAPKTRVAAR
FAGYVEKRRGGARMAAETVAEIIRS

Sequences:

>Translated_425_residues
MTLSARHRLLLSLYGAAWRLCRPLLARHRRLRHGFDERLAPRGRTPQADLWIQSASVGESYLAWQLLRHLPQGSAGSVLL
TTCTVQGRGILDSAAAWCAEHRPDMRVEVRFFPLDEPAVMRRALQLTGAHAVVLLETELWPALMAACAQAGVPYVVVNGR
MTPGSLAGYLNFDGFLRAVPPAAVLAMSEHDAARYAVLFGHGRVRVMPNIKFDRVPQGAAQGPNPLLGTVFRPGTSVAVL
ASVRREEEEDAEYLLRAVAGERPRTCIALVPRHLERVARWRIRLDEAGMPWVLRSAVREPVAPGTVVLWDAFGELDHVYA
LARGVFVGGSLRPLGGQNFLEPLAHGIVPVIGPHWSNFYWVGDDIMEQGLVQRTGGPEEAAQELLRLLRRPAPKTRVAAR
FAGYVEKRRGGARMAAETVAEIIRS
>Mature_424_residues
TLSARHRLLLSLYGAAWRLCRPLLARHRRLRHGFDERLAPRGRTPQADLWIQSASVGESYLAWQLLRHLPQGSAGSVLLT
TCTVQGRGILDSAAAWCAEHRPDMRVEVRFFPLDEPAVMRRALQLTGAHAVVLLETELWPALMAACAQAGVPYVVVNGRM
TPGSLAGYLNFDGFLRAVPPAAVLAMSEHDAARYAVLFGHGRVRVMPNIKFDRVPQGAAQGPNPLLGTVFRPGTSVAVLA
SVRREEEEDAEYLLRAVAGERPRTCIALVPRHLERVARWRIRLDEAGMPWVLRSAVREPVAPGTVVLWDAFGELDHVYAL
ARGVFVGGSLRPLGGQNFLEPLAHGIVPVIGPHWSNFYWVGDDIMEQGLVQRTGGPEEAAQELLRLLRRPAPKTRVAARF
AGYVEKRRGGARMAAETVAEIIRS

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=334, Percent_Identity=32.9341317365269, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 46835; Mature: 46704

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSARHRLLLSLYGAAWRLCRPLLARHRRLRHGFDERLAPRGRTPQADLWIQSASVGES
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHEEEECCCCCHH
YLAWQLLRHLPQGSAGSVLLTTCTVQGRGILDSAAAWCAEHRPDMRVEVRFFPLDEPAVM
HHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEECCCHHHH
RRALQLTGAHAVVLLETELWPALMAACAQAGVPYVVVNGRMTPGSLAGYLNFDGFLRAVP
HHHHHHCCCEEEEEEECHHHHHHHHHHHHCCCCEEEEECCCCCCHHEEEECCCCHHHHCC
PAAVLAMSEHDAARYAVLFGHGRVRVMPNIKFDRVPQGAAQGPNPLLGTVFRPGTSVAVL
HHHEEEECCCCCCEEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCEECEECCCCCHHHH
ASVRREEEEDAEYLLRAVAGERPRTCIALVPRHLERVARWRIRLDEAGMPWVLRSAVREP
HHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCC
VAPGTVVLWDAFGELDHVYALARGVFVGGSLRPLGGQNFLEPLAHGIVPVIGPHWSNFYW
CCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEECCCCCCCEEE
VGDDIMEQGLVQRTGGPEEAAQELLRLLRRPAPKTRVAARFAGYVEKRRGGARMAAETVA
ECHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
EIIRS
HHHCC
>Mature Secondary Structure 
TLSARHRLLLSLYGAAWRLCRPLLARHRRLRHGFDERLAPRGRTPQADLWIQSASVGES
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHEEEECCCCCHH
YLAWQLLRHLPQGSAGSVLLTTCTVQGRGILDSAAAWCAEHRPDMRVEVRFFPLDEPAVM
HHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEECCCHHHH
RRALQLTGAHAVVLLETELWPALMAACAQAGVPYVVVNGRMTPGSLAGYLNFDGFLRAVP
HHHHHHCCCEEEEEEECHHHHHHHHHHHHCCCCEEEEECCCCCCHHEEEECCCCHHHHCC
PAAVLAMSEHDAARYAVLFGHGRVRVMPNIKFDRVPQGAAQGPNPLLGTVFRPGTSVAVL
HHHEEEECCCCCCEEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCEECEECCCCCHHHH
ASVRREEEEDAEYLLRAVAGERPRTCIALVPRHLERVARWRIRLDEAGMPWVLRSAVREP
HHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCC
VAPGTVVLWDAFGELDHVYALARGVFVGGSLRPLGGQNFLEPLAHGIVPVIGPHWSNFYW
CCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEECCCCCCCEEE
VGDDIMEQGLVQRTGGPEEAAQELLRLLRRPAPKTRVAARFAGYVEKRRGGARMAAETVA
ECHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
EIIRS
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]