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The map label for this gene is ddl

Identifier: 78355413

GI number: 78355413

Start: 357058

End: 357984

Strand: Direct

Name: ddl

Synonym: Dde_0366

Alternate gene names: 78355413

Gene position: 357058-357984 (Clockwise)

Preceding gene: 78355412

Following gene: 78355414

Centisome position: 9.57

GC content: 63.0

Gene sequence:

>927_bases
ATGCGTATTCTTTTGATAGCCGGGGGCTGGTCTGAGGAACGGGACGTATCGCTTTCCGGTGCGCGGGGCATTCACGCCGC
GCTGGAGCGGCTGGGGCATCAGGTTACTCTTTTTGACCCCTGCCGGACGCTTGCGGGCCTTCTGGAGGCTGCACAGGCAC
ATGATTTCGCCTTTTTAAACCTGCACGGGCAACCGGGAGAAGACGGCCTTGTGCAGGCGCTGCTGGAAACGGCCGGCGTG
CCGTATCAGGGGTCCGGCCCTGCGGGGTCTTTTCTGGCGCTGAACAAGGCCGCGGCAAAGGAAGTGTTTGTCAGAAACGG
CCTGCCCACACCGGAATGGGTTTTTCTGCCGGCGCATCCGGGAGCGGACTGGGAGCCGCCTTTCGCTTTTCCGGCATTTA
TCAAGTCCAACAACGGCGGATCAAGCCTTGCTCTGCACCGCGTCAGCTGCCCGGGCGAGCTTGCCCGCGCGCTTGACGAG
CTTTTCACCCGCGGCGGCGAGGCCATTATAGAACCCGCCGTGGAAGGCGTGGAGGTGACTTGCGGTGTGCTGGGCGACGA
AGCCTTGCCGCCCATTCTCATCCGTCCGCTGGGCGCCGGATTTTTTGATTATGCCAGCAAATACACTCCGGGGCAGGCGG
AAGAACTGTGTCCCGCGCCGCTGCCCGGAGAAGTTACGGCCAAGGTGCGCGAATACGCGCTGCGGGCGCACCGCGCACTG
GGGCTGCGCGGTTACAGCCGTTCAGATTTTATTCTGACGCCTGCCGGAGCATTGTCCCTGCTGGAAGTGAACACGCTGCC
GGGCATGACGGCCACAAGCCTGCTGCCGCAGGAAGCGGCTGCCGTGGGCATTTCCTTTGACGGGTTGATCGGGCGGCTTA
TCGAGCTGGGACTGGCCGCCCACGGCAAACAACAGGAGAAGGCATGA

Upstream 100 bases:

>100_bases
TACGGCAGAACGGAAAAAAGCAGGCAGTACATAACCCTGTCGCTGGAACAGGCTGCAGATATTGAGCGCGCTCTTGCAGA
GCGACTGGAGATAGATTTCA

Downstream 100 bases:

>100_bases
CCCTGAGCGCAAGACACCGTCTGCTGCTTTCGCTGTATGGCGCGGCATGGCGGTTGTGCCGCCCCCTGCTGGCACGTCAC
CGCCGGCTGCGGCACGGTTT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MRILLIAGGWSEERDVSLSGARGIHAALERLGHQVTLFDPCRTLAGLLEAAQAHDFAFLNLHGQPGEDGLVQALLETAGV
PYQGSGPAGSFLALNKAAAKEVFVRNGLPTPEWVFLPAHPGADWEPPFAFPAFIKSNNGGSSLALHRVSCPGELARALDE
LFTRGGEAIIEPAVEGVEVTCGVLGDEALPPILIRPLGAGFFDYASKYTPGQAEELCPAPLPGEVTAKVREYALRAHRAL
GLRGYSRSDFILTPAGALSLLEVNTLPGMTATSLLPQEAAAVGISFDGLIGRLIELGLAAHGKQQEKA

Sequences:

>Translated_308_residues
MRILLIAGGWSEERDVSLSGARGIHAALERLGHQVTLFDPCRTLAGLLEAAQAHDFAFLNLHGQPGEDGLVQALLETAGV
PYQGSGPAGSFLALNKAAAKEVFVRNGLPTPEWVFLPAHPGADWEPPFAFPAFIKSNNGGSSLALHRVSCPGELARALDE
LFTRGGEAIIEPAVEGVEVTCGVLGDEALPPILIRPLGAGFFDYASKYTPGQAEELCPAPLPGEVTAKVREYALRAHRAL
GLRGYSRSDFILTPAGALSLLEVNTLPGMTATSLLPQEAAAVGISFDGLIGRLIELGLAAHGKQQEKA
>Mature_308_residues
MRILLIAGGWSEERDVSLSGARGIHAALERLGHQVTLFDPCRTLAGLLEAAQAHDFAFLNLHGQPGEDGLVQALLETAGV
PYQGSGPAGSFLALNKAAAKEVFVRNGLPTPEWVFLPAHPGADWEPPFAFPAFIKSNNGGSSLALHRVSCPGELARALDE
LFTRGGEAIIEPAVEGVEVTCGVLGDEALPPILIRPLGAGFFDYASKYTPGQAEELCPAPLPGEVTAKVREYALRAHRAL
GLRGYSRSDFILTPAGALSLLEVNTLPGMTATSLLPQEAAAVGISFDGLIGRLIELGLAAHGKQQEKA

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=306, Percent_Identity=35.2941176470588, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1786579, Length=355, Percent_Identity=29.0140845070423, Blast_Score=134, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_DESDG (Q316H9)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_386862.1
- ProteinModelPortal:   Q316H9
- SMR:   Q316H9
- STRING:   Q316H9
- GeneID:   3756337
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_0366
- NMPDR:   fig|207559.3.peg.1876
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   DEFIVEL
- ProtClustDB:   CLSK704556
- BioCyc:   DDES207559:DDE_0366-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 32382; Mature: 32382

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILLIAGGWSEERDVSLSGARGIHAALERLGHQVTLFDPCRTLAGLLEAAQAHDFAFLN
CEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCEEEEE
LHGQPGEDGLVQALLETAGVPYQGSGPAGSFLALNKAAAKEVFVRNGLPTPEWVFLPAHP
ECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCEEEEECCC
GADWEPPFAFPAFIKSNNGGSSLALHRVSCPGELARALDELFTRGGEAIIEPAVEGVEVT
CCCCCCCCCCHHEEECCCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHEEHHCCCCEEEE
CGVLGDEALPPILIRPLGAGFFDYASKYTPGQAEELCPAPLPGEVTAKVREYALRAHRAL
EEECCCCCCCCEEEEECCCCHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHH
GLRGYSRSDFILTPAGALSLLEVNTLPGMTATSLLPQEAAAVGISFDGLIGRLIELGLAA
CCCCCCCCCEEEECCCCEEEEEECCCCCCHHHHHCCHHHHEECCCHHHHHHHHHHHHHHH
HGKQQEKA
CCCCCCCC
>Mature Secondary Structure
MRILLIAGGWSEERDVSLSGARGIHAALERLGHQVTLFDPCRTLAGLLEAAQAHDFAFLN
CEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCEEEEE
LHGQPGEDGLVQALLETAGVPYQGSGPAGSFLALNKAAAKEVFVRNGLPTPEWVFLPAHP
ECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCEEEEECCC
GADWEPPFAFPAFIKSNNGGSSLALHRVSCPGELARALDELFTRGGEAIIEPAVEGVEVT
CCCCCCCCCCHHEEECCCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHEEHHCCCCEEEE
CGVLGDEALPPILIRPLGAGFFDYASKYTPGQAEELCPAPLPGEVTAKVREYALRAHRAL
EEECCCCCCCCEEEEECCCCHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHH
GLRGYSRSDFILTPAGALSLLEVNTLPGMTATSLLPQEAAAVGISFDGLIGRLIELGLAA
CCCCCCCCCEEEECCCCEEEEEECCCCCCHHHHHCCHHHHEECCCHHHHHHHHHHHHHHH
HGKQQEKA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA