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The map label for this gene is ilvE [H]

Identifier: 78355302

GI number: 78355302

Start: 237561

End: 238502

Strand: Direct

Name: ilvE [H]

Synonym: Dde_0255

Alternate gene names: 78355302

Gene position: 237561-238502 (Clockwise)

Preceding gene: 78355301

Following gene: 304570576

Centisome position: 6.37

GC content: 60.19

Gene sequence:

>942_bases
TTGATACCTGTTCTTGATACCGATGCGTGGGTGGAAAAGCTGCTTTCGACACGCCGCAGTGCCGAAGGCAAGATTCTTGC
GTTTTACGAACACCGCATGGAGGCCATCTGCCGTGACCCGCGTCTGCTGCTGATGCCGGCCGATGATCACCTTGCCCACC
GCGGCGACGGTGTTTTTGAAAGCATGAAGTTTGAAGGGCGCAGGCTGTACCAGCTGGATGCCCATCTGGAGCGCATGGAG
CGCTCGGCGCGGGGGATATACCTGACACCCCCATGCACGCAGAGCGTGCTGCGCGACATCGTGCTGCAGGTAGCCCGCGC
AGCGGACACGCCCGACGGCTGCATACGCATTCTGCTGGGACGTGGTCCCGGCGGGTTCGGCATCGACCCTGCCGAGTGCC
CTGTGCCCAGCCTGTACGTTGTGGCGTACAGCCTTACACCCAAGCCCGAGGAATGGTATGAAAAGGGCGTTACCGCGTTC
CGGACGTCCATTCCCGCCAAACAGGGCTATCTGGCCACCATCAAGAACGCCAACTATATTCCCAACGTGCTCATGAAGCT
GGAGGCCCGTTCCCGCGGCTGCGACATTCCCGTCAGTTTCGACGAGAATGATTTCATTGCCGAAGGCCCCACGGAAAACC
TCTGCCTTGTGGACGCCGACGGCACGCTGGTTGTTCCCGAATTCACCAACGCGCTTTCCGGCACCACCATCATGCGTGTC
ATTGATCTGGTGCGTGATGAAATGAATGTGGCATTCCGCAAGGTGCGCGAGGATGAACTGTACCGCGCCGCAGAAGTCAT
GGTGCTGGGCACCACGACGGAATGTGTGAGTGTTGTCTCCTTTGAGGGGCAGCCCGTGGGCGGGGGCAGACCCGGCCCTG
TGGCACGCCGTTTCCGCGAGCTCCTGCGCAGGGATCTTGCGGAGAACGGTATTTCTTTTTAA

Upstream 100 bases:

>100_bases
CGCACTTAATGCCGTACAGATTGCCGAAGAACTGGTGGAGCGTTCTCTGCTGGATATCGCCGATCCCGGAAAATTCCTGT
AATACAACGGAGCAGTTGCG

Downstream 100 bases:

>100_bases
CCGGAAGCCTGCAGAAGGCAAACGCCCTGCACCCGCAGAACATACCGGGGCCGCGCCGCAAGCCGGCGCGGCCCTGTTTT
CTCGGGGCGGGCAAGAACGC

Product: branched chain amino acid: 2-keto-4-methylthiobutyrate aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MIPVLDTDAWVEKLLSTRRSAEGKILAFYEHRMEAICRDPRLLLMPADDHLAHRGDGVFESMKFEGRRLYQLDAHLERME
RSARGIYLTPPCTQSVLRDIVLQVARAADTPDGCIRILLGRGPGGFGIDPAECPVPSLYVVAYSLTPKPEEWYEKGVTAF
RTSIPAKQGYLATIKNANYIPNVLMKLEARSRGCDIPVSFDENDFIAEGPTENLCLVDADGTLVVPEFTNALSGTTIMRV
IDLVRDEMNVAFRKVREDELYRAAEVMVLGTTTECVSVVSFEGQPVGGGRPGPVARRFRELLRRDLAENGISF

Sequences:

>Translated_313_residues
MIPVLDTDAWVEKLLSTRRSAEGKILAFYEHRMEAICRDPRLLLMPADDHLAHRGDGVFESMKFEGRRLYQLDAHLERME
RSARGIYLTPPCTQSVLRDIVLQVARAADTPDGCIRILLGRGPGGFGIDPAECPVPSLYVVAYSLTPKPEEWYEKGVTAF
RTSIPAKQGYLATIKNANYIPNVLMKLEARSRGCDIPVSFDENDFIAEGPTENLCLVDADGTLVVPEFTNALSGTTIMRV
IDLVRDEMNVAFRKVREDELYRAAEVMVLGTTTECVSVVSFEGQPVGGGRPGPVARRFRELLRRDLAENGISF
>Mature_313_residues
MIPVLDTDAWVEKLLSTRRSAEGKILAFYEHRMEAICRDPRLLLMPADDHLAHRGDGVFESMKFEGRRLYQLDAHLERME
RSARGIYLTPPCTQSVLRDIVLQVARAADTPDGCIRILLGRGPGGFGIDPAECPVPSLYVVAYSLTPKPEEWYEKGVTAF
RTSIPAKQGYLATIKNANYIPNVLMKLEARSRGCDIPVSFDENDFIAEGPTENLCLVDADGTLVVPEFTNALSGTTIMRV
IDLVRDEMNVAFRKVREDELYRAAEVMVLGTTTECVSVVSFEGQPVGGGRPGPVARRFRELLRRDLAENGISF

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=264, Percent_Identity=31.8181818181818, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34836; Mature: 34836

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVLDTDAWVEKLLSTRRSAEGKILAFYEHRMEAICRDPRLLLMPADDHLAHRGDGVFE
CCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCEEEEECCHHHHHCCCCHHH
SMKFEGRRLYQLDAHLERMERSARGIYLTPPCTQSVLRDIVLQVARAADTPDGCIRILLG
HHHCCCCEEEHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
RGPGGFGIDPAECPVPSLYVVAYSLTPKPEEWYEKGVTAFRTSIPAKQGYLATIKNANYI
CCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCHHHHHHCCCCCCCCEEEECCCCCH
PNVLMKLEARSRGCDIPVSFDENDFIAEGPTENLCLVDADGTLVVPEFTNALSGTTIMRV
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEECCCHHHHHCHHHHHHH
IDLVRDEMNVAFRKVREDELYRAAEVMVLGTTTECVSVVSFEGQPVGGGRPGPVARRFRE
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHEECCCCCCCCCCCHHHHHHHH
LLRRDLAENGISF
HHHHHHHHCCCCC
>Mature Secondary Structure
MIPVLDTDAWVEKLLSTRRSAEGKILAFYEHRMEAICRDPRLLLMPADDHLAHRGDGVFE
CCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCEEEEECCHHHHHCCCCHHH
SMKFEGRRLYQLDAHLERMERSARGIYLTPPCTQSVLRDIVLQVARAADTPDGCIRILLG
HHHCCCCEEEHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
RGPGGFGIDPAECPVPSLYVVAYSLTPKPEEWYEKGVTAFRTSIPAKQGYLATIKNANYI
CCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCHHHHHHCCCCCCCCEEEECCCCCH
PNVLMKLEARSRGCDIPVSFDENDFIAEGPTENLCLVDADGTLVVPEFTNALSGTTIMRV
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEECCCHHHHHCHHHHHHH
IDLVRDEMNVAFRKVREDELYRAAEVMVLGTTTECVSVVSFEGQPVGGGRPGPVARRFRE
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHEECCCCCCCCCCCHHHHHHHH
LLRRDLAENGISF
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]