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The map label for this gene is fixL [H]

Identifier: 78355286

GI number: 78355286

Start: 223266

End: 224963

Strand: Direct

Name: fixL [H]

Synonym: Dde_0239

Alternate gene names: 78355286

Gene position: 223266-224963 (Clockwise)

Preceding gene: 78355285

Following gene: 78355293

Centisome position: 5.99

GC content: 61.37

Gene sequence:

>1698_bases
GTGCAGGTGGTCATGAGCCAGCTACAAGATGAAATCATCCTTTTTGCCCCCGATGACGCACATGAGGCACCGCGGCAGTC
CGCCGGCGGTGCATGGGATATCCTTGTGGTGGATGACGACGAGGCTGTCCATGCGGTGACCCGCATGGTGCTGCAGCATC
TGGTGTTTGACGGGCGCAGCATACGGCTTGATCATGCCGGCAGTGCGGCCGGTGCGGAAGAGAAGCTCCGCCGGAACACC
TATGCTCTGGTTCTTCTTGATGTGGTGATGGAGACCGATAACGCCGGACTGGAGCTTGTGCGCTGCATCCGTGATCTTCC
GGCCCTTGCGGAGACCAGAATAGTGCTCTGCACCGGGCATCCGGGACAGGCGCCGGAAGAGCGCATCATGATGGATTACG
ACATAGATGATTACCGTGACAAAAGCGGACTGACGTCGCAGCGGCTCAAGACTGCCGTGTTTGCCGCTCTGCGCAGTCAT
CAGCGGCTGTGCGAGAACAGAGACCAGCGGCTGGAGCTGGCCCGCAGCCGCAACCGCCTGCAGGCCCTCATGGGGGCGCT
GCCTTCGGCCATTGTGGGGATAAGCGCGGGGGGGCACATCATCTTGTGGAATCCTGCGGCGGAACGGCTTTCCGGCCTGC
CCGAGGGCGAAGTGACCGGCCGTGACTTTGCGCAGCCGCTTGAGCAGCTGTCCGTAGATGCTGCTCTGCTGCACCGCGTC
ATGCAGGACGGCGAAACCTACACGCGCCTGCGCGAACGGGTGACTCTGCCGCAGGGCACAACCATCGTGCGGGACCTGCA
TATCCACCCCGTGCCGCAGGCAGGGGGTGCGGTTGTGCGGCTGGACGATGTTTCGGTGCGTGACCGTATGGAAAACCTGA
TGGTGCAGAGCGAAAAAATGCTCTCGCTGGGCAGCATGGCCGCAGGTATGGCGCATGAACTGAACAATCCACTGGGGGCA
GTGCTGCAGGGGGTGGATACCGTGCGCCGCAGACTGGACGGCGCTCTGCCCGCCAACGTGCGGGCCGCGCACGAAACGGG
TCTTACGCTGGCCGCTCTGGGACGTTACATGGAACAGCGCAGGATTCTGTATTTTTTGGACGGCATAGAACATGCCGGAC
GCCGTGCCGCCGTCATTGTGCGTGATATGCTGGATTTCAGCCGCCAGAGCGGTGAAAAACAGCCCGCAGACGTGCACCGG
CTTATTGCTGCCGCGCTGGAACTGGCCGAAAAAAGTTATGATCTGCACGACGCATGGGATTTCCGCAATGTGCAGGTGGA
GCGCTGTTTCGCGTCGTCGCTGCCGCAGATTCCCTGCGCTGCGCAGGAAATTGAACAGGTGCTGCTGAACCTGTTTCACA
ACGCCGCGCAGGCCATGACTCCCGCAGTCATGCAGAACAGAACCCCGCGGCTGTGCATCCGCACGGCTGCTCTGGAGACG
GGCGTGTGCATAGAAGTGGAAGACAACGGGCCGGGAATCCCCGAAGACATCTCAAAACGGGTGTTTGATCCGTTTTTTAC
CACCAAGCCGCCGGGCAAAGGTACCGGACTGGGCCTCTCAGTTTCTTTTCATATCGTGGTGGACCGCCACGGTGGAGAGT
TTCATATGATTTCTCCGGAATCGGGCGGAGCCTGCTTCCGCATACTGCTGCCGTACACAGCCGGCCGTGGCGCGGGGGCA
CAGCAGCAGGCCTGTTGA

Upstream 100 bases:

>100_bases
CACGCGGTTATAACAGTTATACTCGACGTTGACCCGCGCGGAGGATACTGTGGCAAGGTCTGTAATCTGAAACCGGCAGC
ACCGTTTCAGCGGGTGCGCC

Downstream 100 bases:

>100_bases
AGCCGCAGGCAGTCATGCGCCATGGGGAACTGCCATGACCATGGGGTCCACGGCAGCGTCAAATTCTTCTGCCGAAATAA
GTTCCAGTTCCAGACAGGCC

Product: PAS/PAC sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MQVVMSQLQDEIILFAPDDAHEAPRQSAGGAWDILVVDDDEAVHAVTRMVLQHLVFDGRSIRLDHAGSAAGAEEKLRRNT
YALVLLDVVMETDNAGLELVRCIRDLPALAETRIVLCTGHPGQAPEERIMMDYDIDDYRDKSGLTSQRLKTAVFAALRSH
QRLCENRDQRLELARSRNRLQALMGALPSAIVGISAGGHIILWNPAAERLSGLPEGEVTGRDFAQPLEQLSVDAALLHRV
MQDGETYTRLRERVTLPQGTTIVRDLHIHPVPQAGGAVVRLDDVSVRDRMENLMVQSEKMLSLGSMAAGMAHELNNPLGA
VLQGVDTVRRRLDGALPANVRAAHETGLTLAALGRYMEQRRILYFLDGIEHAGRRAAVIVRDMLDFSRQSGEKQPADVHR
LIAAALELAEKSYDLHDAWDFRNVQVERCFASSLPQIPCAAQEIEQVLLNLFHNAAQAMTPAVMQNRTPRLCIRTAALET
GVCIEVEDNGPGIPEDISKRVFDPFFTTKPPGKGTGLGLSVSFHIVVDRHGGEFHMISPESGGACFRILLPYTAGRGAGA
QQQAC

Sequences:

>Translated_565_residues
MQVVMSQLQDEIILFAPDDAHEAPRQSAGGAWDILVVDDDEAVHAVTRMVLQHLVFDGRSIRLDHAGSAAGAEEKLRRNT
YALVLLDVVMETDNAGLELVRCIRDLPALAETRIVLCTGHPGQAPEERIMMDYDIDDYRDKSGLTSQRLKTAVFAALRSH
QRLCENRDQRLELARSRNRLQALMGALPSAIVGISAGGHIILWNPAAERLSGLPEGEVTGRDFAQPLEQLSVDAALLHRV
MQDGETYTRLRERVTLPQGTTIVRDLHIHPVPQAGGAVVRLDDVSVRDRMENLMVQSEKMLSLGSMAAGMAHELNNPLGA
VLQGVDTVRRRLDGALPANVRAAHETGLTLAALGRYMEQRRILYFLDGIEHAGRRAAVIVRDMLDFSRQSGEKQPADVHR
LIAAALELAEKSYDLHDAWDFRNVQVERCFASSLPQIPCAAQEIEQVLLNLFHNAAQAMTPAVMQNRTPRLCIRTAALET
GVCIEVEDNGPGIPEDISKRVFDPFFTTKPPGKGTGLGLSVSFHIVVDRHGGEFHMISPESGGACFRILLPYTAGRGAGA
QQQAC
>Mature_565_residues
MQVVMSQLQDEIILFAPDDAHEAPRQSAGGAWDILVVDDDEAVHAVTRMVLQHLVFDGRSIRLDHAGSAAGAEEKLRRNT
YALVLLDVVMETDNAGLELVRCIRDLPALAETRIVLCTGHPGQAPEERIMMDYDIDDYRDKSGLTSQRLKTAVFAALRSH
QRLCENRDQRLELARSRNRLQALMGALPSAIVGISAGGHIILWNPAAERLSGLPEGEVTGRDFAQPLEQLSVDAALLHRV
MQDGETYTRLRERVTLPQGTTIVRDLHIHPVPQAGGAVVRLDDVSVRDRMENLMVQSEKMLSLGSMAAGMAHELNNPLGA
VLQGVDTVRRRLDGALPANVRAAHETGLTLAALGRYMEQRRILYFLDGIEHAGRRAAVIVRDMLDFSRQSGEKQPADVHR
LIAAALELAEKSYDLHDAWDFRNVQVERCFASSLPQIPCAAQEIEQVLLNLFHNAAQAMTPAVMQNRTPRLCIRTAALET
GVCIEVEDNGPGIPEDISKRVFDPFFTTKPPGKGTGLGLSVSFHIVVDRHGGEFHMISPESGGACFRILLPYTAGRGAGA
QQQAC

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=401, Percent_Identity=26.6832917705736, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1790436, Length=271, Percent_Identity=29.520295202952, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1790300, Length=374, Percent_Identity=25.668449197861, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI87082128, Length=253, Percent_Identity=23.3201581027668, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 62006; Mature: 62006

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50112 PAS ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVVMSQLQDEIILFAPDDAHEAPRQSAGGAWDILVVDDDEAVHAVTRMVLQHLVFDGRS
CHHHHHHCCCCEEEECCCCHHHCCHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCE
IRLDHAGSAAGAEEKLRRNTYALVLLDVVMETDNAGLELVRCIRDLPALAETRIVLCTGH
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCEEEEEECC
PGQAPEERIMMDYDIDDYRDKSGLTSQRLKTAVFAALRSHQRLCENRDQRLELARSRNRL
CCCCCHHCEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
QALMGALPSAIVGISAGGHIILWNPAAERLSGLPEGEVTGRDFAQPLEQLSVDAALLHRV
HHHHHHHHHHHEEECCCCEEEEECCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
MQDGETYTRLRERVTLPQGTTIVRDLHIHPVPQAGGAVVRLDDVSVRDRMENLMVQSEKM
HHCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
LSLGSMAAGMAHELNNPLGAVLQGVDTVRRRLDGALPANVRAAHETGLTLAALGRYMEQR
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHC
RILYFLDGIEHAGRRAAVIVRDMLDFSRQSGEKQPADVHRLIAAALELAEKSYDLHDAWD
CHHHEEHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
FRNVQVERCFASSLPQIPCAAQEIEQVLLNLFHNAAQAMTPAVMQNRTPRLCIRTAALET
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHEEEHHHHCC
GVCIEVEDNGPGIPEDISKRVFDPFFTTKPPGKGTGLGLSVSFHIVVDRHGGEFHMISPE
CCEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCEEEEECCC
SGGACFRILLPYTAGRGAGAQQQAC
CCCCEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MQVVMSQLQDEIILFAPDDAHEAPRQSAGGAWDILVVDDDEAVHAVTRMVLQHLVFDGRS
CHHHHHHCCCCEEEECCCCHHHCCHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCE
IRLDHAGSAAGAEEKLRRNTYALVLLDVVMETDNAGLELVRCIRDLPALAETRIVLCTGH
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCEEEEEECC
PGQAPEERIMMDYDIDDYRDKSGLTSQRLKTAVFAALRSHQRLCENRDQRLELARSRNRL
CCCCCHHCEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
QALMGALPSAIVGISAGGHIILWNPAAERLSGLPEGEVTGRDFAQPLEQLSVDAALLHRV
HHHHHHHHHHHEEECCCCEEEEECCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
MQDGETYTRLRERVTLPQGTTIVRDLHIHPVPQAGGAVVRLDDVSVRDRMENLMVQSEKM
HHCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
LSLGSMAAGMAHELNNPLGAVLQGVDTVRRRLDGALPANVRAAHETGLTLAALGRYMEQR
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHC
RILYFLDGIEHAGRRAAVIVRDMLDFSRQSGEKQPADVHRLIAAALELAEKSYDLHDAWD
CHHHEEHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
FRNVQVERCFASSLPQIPCAAQEIEQVLLNLFHNAAQAMTPAVMQNRTPRLCIRTAALET
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHEEEHHHHCC
GVCIEVEDNGPGIPEDISKRVFDPFFTTKPPGKGTGLGLSVSFHIVVDRHGGEFHMISPE
CCEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCEEEEECCC
SGGACFRILLPYTAGRGAGAQQQAC
CCCCEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]