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The map label for this gene is purA

Identifier: 78355223

GI number: 78355223

Start: 161700

End: 162983

Strand: Direct

Name: purA

Synonym: Dde_0176

Alternate gene names: 78355223

Gene position: 161700-162983 (Clockwise)

Preceding gene: 304570602

Following gene: 78355224

Centisome position: 4.33

GC content: 59.03

Gene sequence:

>1284_bases
ATGTCAAACGTGGTAATCATGGGAGCACAGTGGGGCGACGAAGGAAAAGGTAAGATCGTCGACCTGCTTTCCCGGGAAAT
AGACGTCATCGTCCGCTTCCAGGGCGGAAACAACGCGGGCCACACCGTTATTGTGGGTGACAAGTCCTACATACTGCACC
TTATTCCCTCCGGCATTCTGCACGAAGGCAAAACGTGCCTTATCGGCAACGGAGTGGTGCTGGACCCCGTGGTGTTCTGG
AAGGAAGTGGAAGCCCTTGCAGAGCGTGGTATCGACGTGGGCCCCCAGAGGCTCATGATCAGCCGCAAGGCCCATGTGAT
CATGCCTTATCACAAGGCGCTTGATGTCGCCCGTGAGGACTTTAAAAACAAAGAAAGCAGGATCGGCACCACAGGCCGTG
GCATTGGTCCCTGCTACGAAGATAAAATGTCGCGCATCGGCGTGCGTGCCGGCGATCTGGCCGACCCCGCACTGCTGCGC
TCGAAGATAGAAGCCGCTCTGGTGGAAAAGAACGCCCTGTTCACGGCATTGTACGGGCGCGAGGCCATGTCCGTGGATGC
GGTGTTCGACGAAGTGATGGCCGTGGGCGGCCGTCTGGTGCCGTATCTGACCGATGTGTCTTCGGTTATCGAAGATGCAT
GGGCAAATGATTCCGGCGTCATGTTCGAGGGCGCGCAGGGAACGCATCTGGATATCGACCACGGTACATATCCTTTTGTG
ACCTCTTCCAACACCGTGGCCGGCAACGCCGCCGCGGGCAGCGGTATTCCCGCTTCGCGTCTGGACCGTGTGGTGGCCAT
AGTAAAAGCGTACACCACCCGTGTGGGCGCAGGCCCCTTCCCCACCGAGCTTGATGACGAAGCCGGCAACCACATGCAGA
GCGTGGGGCACGAATTCGGCGCCACCACCGGCCGCAAGCGTCGCTGCGGGTGGCTGGACTGCGCCCTGCTGCGCGAATCG
GTGCGCCTGAACGGTCCCACCGACATAGCTCTGACCAAGCTGGATGTCATGAGCGGGCTGAAGGAACTGAAAATCTGTGT
GGCTTACGAATACAACGGCCAGCGTCTGCAGCATCCGCCGCAGGAACAGAACGGCCTTGCCCATGTGACACCGGTATATG
AAACCATGCCCGGATGGGATGACGACATAACCGCAGCCAAAACATGGGAAGACCTGCCCGAGGCCGCCCGTAATTATATC
TGCCGTATCGAAGAGCTGCTGGGCGTGCCTGTGTCCATGATTTCCGTGGGTCCCGAACGCGACCAGACGCTCAACAGGAA
ATAA

Upstream 100 bases:

>100_bases
ACTTTTCTATTGGCGGCATGTAGGTAATGATCCCTTATGCCGTGCGCCGAACTCCTGTTTTGCGTCGGAAAGGCGACCTG
CATGTCACAAGGAGATGGAA

Downstream 100 bases:

>100_bases
GACGTGTCCGCTCAGGGTGAAACGCTCTGTGCGCCGCATGCCTCTGATATCATAGCCCCGGCCCTGTCGTCGCGTCACGA
CAGGGTGCGGGGCTATCTTG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSNVVIMGAQWGDEGKGKIVDLLSREIDVIVRFQGGNNAGHTVIVGDKSYILHLIPSGILHEGKTCLIGNGVVLDPVVFW
KEVEALAERGIDVGPQRLMISRKAHVIMPYHKALDVAREDFKNKESRIGTTGRGIGPCYEDKMSRIGVRAGDLADPALLR
SKIEAALVEKNALFTALYGREAMSVDAVFDEVMAVGGRLVPYLTDVSSVIEDAWANDSGVMFEGAQGTHLDIDHGTYPFV
TSSNTVAGNAAAGSGIPASRLDRVVAIVKAYTTRVGAGPFPTELDDEAGNHMQSVGHEFGATTGRKRRCGWLDCALLRES
VRLNGPTDIALTKLDVMSGLKELKICVAYEYNGQRLQHPPQEQNGLAHVTPVYETMPGWDDDITAAKTWEDLPEAARNYI
CRIEELLGVPVSMISVGPERDQTLNRK

Sequences:

>Translated_427_residues
MSNVVIMGAQWGDEGKGKIVDLLSREIDVIVRFQGGNNAGHTVIVGDKSYILHLIPSGILHEGKTCLIGNGVVLDPVVFW
KEVEALAERGIDVGPQRLMISRKAHVIMPYHKALDVAREDFKNKESRIGTTGRGIGPCYEDKMSRIGVRAGDLADPALLR
SKIEAALVEKNALFTALYGREAMSVDAVFDEVMAVGGRLVPYLTDVSSVIEDAWANDSGVMFEGAQGTHLDIDHGTYPFV
TSSNTVAGNAAAGSGIPASRLDRVVAIVKAYTTRVGAGPFPTELDDEAGNHMQSVGHEFGATTGRKRRCGWLDCALLRES
VRLNGPTDIALTKLDVMSGLKELKICVAYEYNGQRLQHPPQEQNGLAHVTPVYETMPGWDDDITAAKTWEDLPEAARNYI
CRIEELLGVPVSMISVGPERDQTLNRK
>Mature_426_residues
SNVVIMGAQWGDEGKGKIVDLLSREIDVIVRFQGGNNAGHTVIVGDKSYILHLIPSGILHEGKTCLIGNGVVLDPVVFWK
EVEALAERGIDVGPQRLMISRKAHVIMPYHKALDVAREDFKNKESRIGTTGRGIGPCYEDKMSRIGVRAGDLADPALLRS
KIEAALVEKNALFTALYGREAMSVDAVFDEVMAVGGRLVPYLTDVSSVIEDAWANDSGVMFEGAQGTHLDIDHGTYPFVT
SSNTVAGNAAAGSGIPASRLDRVVAIVKAYTTRVGAGPFPTELDDEAGNHMQSVGHEFGATTGRKRRCGWLDCALLRESV
RLNGPTDIALTKLDVMSGLKELKICVAYEYNGQRLQHPPQEQNGLAHVTPVYETMPGWDDDITAAKTWEDLPEAARNYIC
RIEELLGVPVSMISVGPERDQTLNRK

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=425, Percent_Identity=43.5294117647059, Blast_Score=348, Evalue=7e-96,
Organism=Homo sapiens, GI34577063, Length=426, Percent_Identity=41.5492957746479, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=41.6666666666667, Blast_Score=303, Evalue=3e-82,
Organism=Escherichia coli, GI1790620, Length=424, Percent_Identity=51.8867924528302, Blast_Score=431, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI25146553, Length=434, Percent_Identity=42.6267281105991, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI25146555, Length=434, Percent_Identity=42.6267281105991, Blast_Score=335, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6324109, Length=434, Percent_Identity=41.9354838709677, Blast_Score=352, Evalue=7e-98,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=40.661938534279, Blast_Score=330, Evalue=2e-90,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_DESDG (Q317B9)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_386672.1
- ProteinModelPortal:   Q317B9
- SMR:   Q317B9
- STRING:   Q317B9
- GeneID:   3755347
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_0176
- NMPDR:   fig|207559.3.peg.832
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BioCyc:   DDES207559:DDE_0176-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46376; Mature: 46245

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 131-131 BINDING 145-145 BINDING 226-226 BINDING 241-241 BINDING 305-305 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVVIMGAQWGDEGKGKIVDLLSREIDVIVRFQGGNNAGHTVIVGDKSYILHLIPSGIL
CCCEEEEECCCCCCCCCHHHHHHHCCEEEEEEEECCCCCCCEEEECCCCEEEEECCCHHH
HEGKTCLIGNGVVLDPVVFWKEVEALAERGIDVGPQRLMISRKAHVIMPYHKALDVARED
HCCCEEEEECCEEECHHHHHHHHHHHHHCCCCCCCHHHEEECCCEEEECHHHHHHHHHHH
FKNKESRIGTTGRGIGPCYEDKMSRIGVRAGDLADPALLRSKIEAALVEKNALFTALYGR
HCCHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCC
EAMSVDAVFDEVMAVGGRLVPYLTDVSSVIEDAWANDSGVMFEGAQGTHLDIDHGTYPFV
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCEE
TSSNTVAGNAAAGSGIPASRLDRVVAIVKAYTTRVGAGPFPTELDDEAGNHMQSVGHEFG
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHC
ATTGRKRRCGWLDCALLRESVRLNGPTDIALTKLDVMSGLKELKICVAYEYNGQRLQHPP
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHCHHHEEEEEEEEECCCCCCCCC
QEQNGLAHVTPVYETMPGWDDDITAAKTWEDLPEAARNYICRIEELLGVPVSMISVGPER
CHHCCCEEECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
DQTLNRK
CCCCCCC
>Mature Secondary Structure 
SNVVIMGAQWGDEGKGKIVDLLSREIDVIVRFQGGNNAGHTVIVGDKSYILHLIPSGIL
CCEEEEECCCCCCCCCHHHHHHHCCEEEEEEEECCCCCCCEEEECCCCEEEEECCCHHH
HEGKTCLIGNGVVLDPVVFWKEVEALAERGIDVGPQRLMISRKAHVIMPYHKALDVARED
HCCCEEEEECCEEECHHHHHHHHHHHHHCCCCCCCHHHEEECCCEEEECHHHHHHHHHHH
FKNKESRIGTTGRGIGPCYEDKMSRIGVRAGDLADPALLRSKIEAALVEKNALFTALYGR
HCCHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCC
EAMSVDAVFDEVMAVGGRLVPYLTDVSSVIEDAWANDSGVMFEGAQGTHLDIDHGTYPFV
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCEE
TSSNTVAGNAAAGSGIPASRLDRVVAIVKAYTTRVGAGPFPTELDDEAGNHMQSVGHEFG
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHC
ATTGRKRRCGWLDCALLRESVRLNGPTDIALTKLDVMSGLKELKICVAYEYNGQRLQHPP
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHCHHHEEEEEEEEECCCCCCCCC
QEQNGLAHVTPVYETMPGWDDDITAAKTWEDLPEAARNYICRIEELLGVPVSMISVGPER
CHHCCCEEECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
DQTLNRK
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA