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The map label for this gene is holB [H]

Identifier: 78355224

GI number: 78355224

Start: 162987

End: 163877

Strand: Direct

Name: holB [H]

Synonym: Dde_0177

Alternate gene names: 78355224

Gene position: 162987-163877 (Clockwise)

Preceding gene: 78355223

Following gene: 78355225

Centisome position: 4.37

GC content: 64.76

Gene sequence:

>891_bases
GTGTCCGCTCAGGGTGAAACGCTCTGTGCGCCGCATGCCTCTGATATCATAGCCCCGGCCCTGTCGTCGCGTCACGACAG
GGTGCGGGGCTATCTTGAGCGGCTTGCTGGCTCTGTGCCGCAGGTGCTGCTGCTGGAAGGCGGCACCCCCCGCGAACGCG
AGGCCGTGGCGCTGTGGTGGGCCGCGCGTCTCAACTGCGCGGGGACCGGTGCGCCCTGCCTTTCCTGCGCTGTCTGCACC
CAGTTTGCCACGCGCAACAACCGCGATATGTTTTATCTGGACGGTGCGCAGGGTTCCATCAAGATTGATGATGTGCGCGC
CGTGCGTGCCGTGCTGGGCGAACCGCCCAGAGGCCGCTACCGTGTGGTTGTGCTGGCCGAGGCTCAGTCGCTGGGCATAG
AGGCCGCCAATGCCCTTCTTAAATCGCTGGAAGAACCCCGTCCCGGAACCAGTTTTGTTCTGCTGGCTCCGCAGCGTGAA
CGTCTGCTGCCAACTCTTGTTTCCCGCAGCTGGGTGCTGACTCTGGCCTGGCCGGACGGCGGCGCCTGCGACCCTGCCGT
GCAGGAATGGGCCGATGCCCTTGCCGGTTTTGTGTCAGGCGAAGAGGGCTGGTTTGCCCGTACCGGTGCCAAGGGCAGCA
TCGATCAGGGAACTGCGTTGCAGCTGGTGGCATTTCTGCAGCGCCAGCTTGCCCTGGCTCTCACCGGCCGCGGGGCGTCT
TCGTTTGCCCGCCTGCTTGTCTCGCGCCTTGATACCGCCGCCATGCGCCGTCTGGATGAAGCGCTGGCGGAGTGTCAGGA
CTCTCTCATTTACAATGTGAATCCCGCTCTTGTCCTTGACTGGCTGGCAACGCGCCTTTTCGCATGGGCGCGTACGTCCC
GCCGCGCTTGA

Upstream 100 bases:

>100_bases
CCGTAATTATATCTGCCGTATCGAAGAGCTGCTGGGCGTGCCTGTGTCCATGATTTCCGTGGGTCCCGAACGCGACCAGA
CGCTCAACAGGAAATAAGAC

Downstream 100 bases:

>100_bases
TTTTCGTTGCCTGCCGGACGTGCTGCCGCTATGGTTGAGGGTGTGCGCGGCCGGTTTTTCCGCGTTCTGCATATCCCTCA
AGGGTTTGGTATGATGAATG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSAQGETLCAPHASDIIAPALSSRHDRVRGYLERLAGSVPQVLLLEGGTPREREAVALWWAARLNCAGTGAPCLSCAVCT
QFATRNNRDMFYLDGAQGSIKIDDVRAVRAVLGEPPRGRYRVVVLAEAQSLGIEAANALLKSLEEPRPGTSFVLLAPQRE
RLLPTLVSRSWVLTLAWPDGGACDPAVQEWADALAGFVSGEEGWFARTGAKGSIDQGTALQLVAFLQRQLALALTGRGAS
SFARLLVSRLDTAAMRRLDEALAECQDSLIYNVNPALVLDWLATRLFAWARTSRRA

Sequences:

>Translated_296_residues
MSAQGETLCAPHASDIIAPALSSRHDRVRGYLERLAGSVPQVLLLEGGTPREREAVALWWAARLNCAGTGAPCLSCAVCT
QFATRNNRDMFYLDGAQGSIKIDDVRAVRAVLGEPPRGRYRVVVLAEAQSLGIEAANALLKSLEEPRPGTSFVLLAPQRE
RLLPTLVSRSWVLTLAWPDGGACDPAVQEWADALAGFVSGEEGWFARTGAKGSIDQGTALQLVAFLQRQLALALTGRGAS
SFARLLVSRLDTAAMRRLDEALAECQDSLIYNVNPALVLDWLATRLFAWARTSRRA
>Mature_295_residues
SAQGETLCAPHASDIIAPALSSRHDRVRGYLERLAGSVPQVLLLEGGTPREREAVALWWAARLNCAGTGAPCLSCAVCTQ
FATRNNRDMFYLDGAQGSIKIDDVRAVRAVLGEPPRGRYRVVVLAEAQSLGIEAANALLKSLEEPRPGTSFVLLAPQRER
LLPTLVSRSWVLTLAWPDGGACDPAVQEWADALAGFVSGEEGWFARTGAKGSIDQGTALQLVAFLQRQLALALTGRGASS
FARLLVSRLDTAAMRRLDEALAECQDSLIYNVNPALVLDWLATRLFAWARTSRRA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 31967; Mature: 31836

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQGETLCAPHASDIIAPALSSRHDRVRGYLERLAGSVPQVLLLEGGTPREREAVALWW
CCCCCCCEECCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHEEEEE
AARLNCAGTGAPCLSCAVCTQFATRNNRDMFYLDGAQGSIKIDDVRAVRAVLGEPPRGRY
EHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHHHHHHHCCCCCCCE
RVVVLAEAQSLGIEAANALLKSLEEPRPGTSFVLLAPQRERLLPTLVSRSWVLTLAWPDG
EEEEEECCHHHCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCC
GACDPAVQEWADALAGFVSGEEGWFARTGAKGSIDQGTALQLVAFLQRQLALALTGRGAS
CCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
SFARLLVSRLDTAAMRRLDEALAECQDSLIYNVNPALVLDWLATRLFAWARTSRRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAQGETLCAPHASDIIAPALSSRHDRVRGYLERLAGSVPQVLLLEGGTPREREAVALWW
CCCCCCEECCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHEEEEE
AARLNCAGTGAPCLSCAVCTQFATRNNRDMFYLDGAQGSIKIDDVRAVRAVLGEPPRGRY
EHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHHHHHHHCCCCCCCE
RVVVLAEAQSLGIEAANALLKSLEEPRPGTSFVLLAPQRERLLPTLVSRSWVLTLAWPDG
EEEEEECCHHHCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCC
GACDPAVQEWADALAGFVSGEEGWFARTGAKGSIDQGTALQLVAFLQRQLALALTGRGAS
CCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
SFARLLVSRLDTAAMRRLDEALAECQDSLIYNVNPALVLDWLATRLFAWARTSRRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]