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The map label for this gene is macA [H]

Identifier: 78224231

GI number: 78224231

Start: 3423571

End: 3424782

Strand: Reverse

Name: macA [H]

Synonym: Gmet_3038

Alternate gene names: 78224231

Gene position: 3424782-3423571 (Counterclockwise)

Preceding gene: 78224233

Following gene: 78224230

Centisome position: 85.67

GC content: 63.37

Gene sequence:

>1212_bases
ATGAAGAAATTCCTCATCCCCGCCGCTGCCGTCCTCGTGGTGGCCGGCGTTGCCGCGTACTTTACCCTGAACAAAAAGCC
GGAAACTGTGTTCAAGACCGCCAAGATCGAGCGGGGGGATATTGTCTCCACTGTCTCCGCCACCGGCAACCTTGCCGCCG
TGGTGACGGTACAGGTGGGTACCCAGGTTTCCGGCACCATCCAGAAGCTCTTTGTGGACTACAACTCGCCGGTGAAAAAG
GGGCAGGTCATCGCCCAGATCGATCCTTCCCTGTTTTCGGCCCAGGTGGAGCAGACCCGGGGAAACTACCTGAACGCCCA
GGCCAACCTCCAGAGGGTGAGGGCTGATCTGGCAGATGCCCGGCGCAACCAGGAGCGGAACCGCCAGCTCCTCAAGGACG
GCGTCGTCTCCCAGAGCGATTTTGACGCCGCCGACAACCGCTACCAGATGGCCCAGGCCACCGTGAAAGCAGCCGAGGGA
AGCGTGGCCCAGACCCGCGGCGCCTTCAGCCAGTCGGAGACCAACCTCCGCTATGCCACCATCCGTTCGCCCGTGGACGG
CATCGTCGTCTCCCGCAACGTGGACGTGGGGCAGACCGTGGCCGCCTCGTTCCAGACCCCGACCCTCTTCACCATCGCCC
AGGACCTGACCCGCATGGAGATCGACACCAGCGTGGACGAGGCCGACATCAGCCGGGTGCAGGTGGGGCAGCCGGTCAGC
TTCACCGTGGACTCCTATCCCGAGAACCGCTTCACCGGCACCGTCCGCCAGGTGCGCAACGCACCGGTGGTCACCCAGAA
CGTCGTGACCTACGTGGTGGTGATCGACGTGGACAACAAGGACATGAAGCTCAAGCCGGGGATGACCGCCAATGTCAGCA
TCGAGACCGCCCGCAAGGACAATGTCCTGAAGCTCCCTTCCGCTGCGCTCCGGTTCCGGCCCAAGAAGGGGGGTGATGAG
AAGGCGGCGAAGGGACAGCAGGCTGGCCAGGAGCAGCGGAGGAAAAAGCGGGAAGGTGGGCAGCAGGTCTACATCCTGGG
GGCGGAGAACAAGCCGGTTCCGGTGCCGGTTAAGACCGGCATCGGCAACGATGGGCAGGTGGAACTGGTTTCCGGGAACC
TCAAGGAAAACGACCAGGTGATCATCGAACAGGTTACCCCGCAGCAGAAAAAAGCGGGCGGCATGGGCGGATCCATGGGG
CCGAGGTTCTAG

Upstream 100 bases:

>100_bases
GACAGGGGGCTTTTTTGTGTCAACCGGTTGAGGTTTGCCTCGTTTTCATCTATACACAAAGCATAATTCCGATAAAAGAT
CATCCTCGCCGAGGTCCCCC

Downstream 100 bases:

>100_bases
ATGGGCGAGGTGGTCAAAATAACCGACGTCACCAAGGTCTATACCATGGGAGACCAGCGGGTGGAGGCCCTGAAGGGGAT
ATCCTTCACGGTCTCCCAAG

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKKFLIPAAAVLVVAGVAAYFTLNKKPETVFKTAKIERGDIVSTVSATGNLAAVVTVQVGTQVSGTIQKLFVDYNSPVKK
GQVIAQIDPSLFSAQVEQTRGNYLNAQANLQRVRADLADARRNQERNRQLLKDGVVSQSDFDAADNRYQMAQATVKAAEG
SVAQTRGAFSQSETNLRYATIRSPVDGIVVSRNVDVGQTVAASFQTPTLFTIAQDLTRMEIDTSVDEADISRVQVGQPVS
FTVDSYPENRFTGTVRQVRNAPVVTQNVVTYVVVIDVDNKDMKLKPGMTANVSIETARKDNVLKLPSAALRFRPKKGGDE
KAAKGQQAGQEQRRKKREGGQQVYILGAENKPVPVPVKTGIGNDGQVELVSGNLKENDQVIIEQVTPQQKKAGGMGGSMG
PRF

Sequences:

>Translated_403_residues
MKKFLIPAAAVLVVAGVAAYFTLNKKPETVFKTAKIERGDIVSTVSATGNLAAVVTVQVGTQVSGTIQKLFVDYNSPVKK
GQVIAQIDPSLFSAQVEQTRGNYLNAQANLQRVRADLADARRNQERNRQLLKDGVVSQSDFDAADNRYQMAQATVKAAEG
SVAQTRGAFSQSETNLRYATIRSPVDGIVVSRNVDVGQTVAASFQTPTLFTIAQDLTRMEIDTSVDEADISRVQVGQPVS
FTVDSYPENRFTGTVRQVRNAPVVTQNVVTYVVVIDVDNKDMKLKPGMTANVSIETARKDNVLKLPSAALRFRPKKGGDE
KAAKGQQAGQEQRRKKREGGQQVYILGAENKPVPVPVKTGIGNDGQVELVSGNLKENDQVIIEQVTPQQKKAGGMGGSMG
PRF
>Mature_403_residues
MKKFLIPAAAVLVVAGVAAYFTLNKKPETVFKTAKIERGDIVSTVSATGNLAAVVTVQVGTQVSGTIQKLFVDYNSPVKK
GQVIAQIDPSLFSAQVEQTRGNYLNAQANLQRVRADLADARRNQERNRQLLKDGVVSQSDFDAADNRYQMAQATVKAAEG
SVAQTRGAFSQSETNLRYATIRSPVDGIVVSRNVDVGQTVAASFQTPTLFTIAQDLTRMEIDTSVDEADISRVQVGQPVS
FTVDSYPENRFTGTVRQVRNAPVVTQNVVTYVVVIDVDNKDMKLKPGMTANVSIETARKDNVLKLPSAALRFRPKKGGDE
KAAKGQQAGQEQRRKKREGGQQVYILGAENKPVPVPVKTGIGNDGQVELVSGNLKENDQVIIEQVTPQQKKAGGMGGSMG
PRF

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=386, Percent_Identity=29.7927461139896, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI87082036, Length=349, Percent_Identity=27.2206303724928, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789929, Length=258, Percent_Identity=27.1317829457364, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789041, Length=297, Percent_Identity=26.9360269360269, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43580; Mature: 43580

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFLIPAAAVLVVAGVAAYFTLNKKPETVFKTAKIERGDIVSTVSATGNLAAVVTVQVG
CCCCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEEC
TQVSGTIQKLFVDYNSPVKKGQVIAQIDPSLFSAQVEQTRGNYLNAQANLQRVRADLADA
CCCHHHHHHHEECCCCCCCCCEEEEEECHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHH
RRNQERNRQLLKDGVVSQSDFDAADNRYQMAQATVKAAEGSVAQTRGAFSQSETNLRYAT
HHCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEE
IRSPVDGIVVSRNVDVGQTVAASFQTPTLFTIAQDLTRMEIDTSVDEADISRVQVGQPVS
ECCCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHEECCCCCCHHHHHHEECCCCEE
FTVDSYPENRFTGTVRQVRNAPVVTQNVVTYVVVIDVDNKDMKLKPGMTANVSIETARKD
EEECCCCCCCHHHHHHHHCCCCCEEECEEEEEEEEEECCCCEEECCCCEEEEEEEECCCC
NVLKLPSAALRFRPKKGGDEKAAKGQQAGQEQRRKKREGGQQVYILGAENKPVPVPVKTG
CEEECCCHHHEECCCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECC
IGNDGQVELVSGNLKENDQVIIEQVTPQQKKAGGMGGSMGPRF
CCCCCEEEEEECCCCCCCEEEEEECCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKKFLIPAAAVLVVAGVAAYFTLNKKPETVFKTAKIERGDIVSTVSATGNLAAVVTVQVG
CCCCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEEC
TQVSGTIQKLFVDYNSPVKKGQVIAQIDPSLFSAQVEQTRGNYLNAQANLQRVRADLADA
CCCHHHHHHHEECCCCCCCCCEEEEEECHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHH
RRNQERNRQLLKDGVVSQSDFDAADNRYQMAQATVKAAEGSVAQTRGAFSQSETNLRYAT
HHCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEE
IRSPVDGIVVSRNVDVGQTVAASFQTPTLFTIAQDLTRMEIDTSVDEADISRVQVGQPVS
ECCCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHEECCCCCCHHHHHHEECCCCEE
FTVDSYPENRFTGTVRQVRNAPVVTQNVVTYVVVIDVDNKDMKLKPGMTANVSIETARKD
EEECCCCCCCHHHHHHHHCCCCCEEECEEEEEEEEEECCCCEEECCCCEEEEEEEECCCC
NVLKLPSAALRFRPKKGGDEKAAKGQQAGQEQRRKKREGGQQVYILGAENKPVPVPVKTG
CEEECCCHHHEECCCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECC
IGNDGQVELVSGNLKENDQVIIEQVTPQQKKAGGMGGSMGPRF
CCCCCEEEEEECCCCCCCEEEEEECCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]