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The map label for this gene is hycF [C]

Identifier: 78224233

GI number: 78224233

Start: 3426420

End: 3427550

Strand: Reverse

Name: hycF [C]

Synonym: Gmet_3040

Alternate gene names: 78224233

Gene position: 3427550-3426420 (Counterclockwise)

Preceding gene: 78224234

Following gene: 78224231

Centisome position: 85.74

GC content: 66.58

Gene sequence:

>1131_bases
GTGAGGCACGCCGTCGCCATCGAGCGGGCCGGGGATTACCAGTCCGACGTGGTCAGGCCTGCTCTGGACCGCCTTCTCGA
ACCCTTTGGGGGGATGGCCTCCTTCGTCAAGCCTGGGGAGAGGGTTCTTCTCAAGCCGAACATGCTTGCTGCGAAGGAGC
CGGAGCGGGCGGTCACGACTCACCCGGAGCTGCTCCGGCAGGTGATCGGGCTCGTGCGGGAGGCGGGTGGGATTCCCCTC
GTGGGTGACTCCCCCGGCGTCGGCGGCATCCGGCGGGTGGCGGAGCGAAGCGGTATCCTTGCCGTGGTGGAGGAGACCGG
GGCCGAGTTCATCCCCTTTGACGAGACGGTGACGGTACGGGGCAAGGGGCTCTTTCGGGAGTTCCAGATCGCCCGCCCCT
ATCTGGAGGCGGACCGCCTCATCAACCTCCCCAAGCTCAAAACCCACGAGATGATGACCATGACCTGCGCCGTGAAGAAC
CTCTTCGGCGCGGTGGTGGGGGCCGCCAAGGCGGGTTGGCACCTGAAGGCGGGGGCCGACCGGGAGCTCTTTGCCCGGAT
GCTCCTGGAGATCTATCTCTTGCGCAGGCCCGACCTGACCATCGTTGACGGCATCCTCGCCATGGAGGGGGACGGACCGG
GAAGCGGCGACCCGCGCCTCTGCGGGCTCCTCCTCGCCGGGACGAACCCGGTGGCGGTGGATGTCATCGCCGCCGAACTG
GCCGGCATCCCGAAGAAGCTCCTTTGGGTGGAGCGGGCCGCCGAGAAGCTCGGCATCGAGGGCTGGGACCGGGCCACCAT
CGACACCCGCGGCGTACCCGTTGACGAAGCGAAGGTTTCCCCCTTCCGTCTTCCCCACATTTCCGATGTCCAGTTCGGCC
TTCCCCGCTTCCTCAAGAACCGGCTTCGCCACTACCTCACCTCGCGTCCCTGTGGCAATCCCGAGGCGTGCACCCTCTGC
GGCATCTGTCGCGATGCCTGCCCCCCCGGGGCCATCACCATCCAGGACGGGAAGCTGGCTTTCGACTACCACGCCTGCAT
CCGCTGCTTCTGCTGCCGGGAACTCTGTCCCGAGGGGGCACTCGGCGTGCGGGAAGGGGCGCTCCTGAAAATGATTAAGA
AATGTGTATAA

Upstream 100 bases:

>100_bases
TGATCGGCTTCCGCAACCGGATCGTCGTCATGCTCAACTGGATCTGGTACTACTGGTTCCACGAACGGCAGGTGCGGCTC
ATCACCGAGCGGGTGGAGGA

Downstream 100 bases:

>100_bases
AGATCACTACTGTCCGGTTAATAGTTGAATAAGTTCAGTTGGTTGCGCTCAGGATAATCAGCATTTCGCTTGAGAGCCTC
CGCAACCAGCGATGAAATGG

Product: hypothetical protein

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MRHAVAIERAGDYQSDVVRPALDRLLEPFGGMASFVKPGERVLLKPNMLAAKEPERAVTTHPELLRQVIGLVREAGGIPL
VGDSPGVGGIRRVAERSGILAVVEETGAEFIPFDETVTVRGKGLFREFQIARPYLEADRLINLPKLKTHEMMTMTCAVKN
LFGAVVGAAKAGWHLKAGADRELFARMLLEIYLLRRPDLTIVDGILAMEGDGPGSGDPRLCGLLLAGTNPVAVDVIAAEL
AGIPKKLLWVERAAEKLGIEGWDRATIDTRGVPVDEAKVSPFRLPHISDVQFGLPRFLKNRLRHYLTSRPCGNPEACTLC
GICRDACPPGAITIQDGKLAFDYHACIRCFCCRELCPEGALGVREGALLKMIKKCV

Sequences:

>Translated_376_residues
MRHAVAIERAGDYQSDVVRPALDRLLEPFGGMASFVKPGERVLLKPNMLAAKEPERAVTTHPELLRQVIGLVREAGGIPL
VGDSPGVGGIRRVAERSGILAVVEETGAEFIPFDETVTVRGKGLFREFQIARPYLEADRLINLPKLKTHEMMTMTCAVKN
LFGAVVGAAKAGWHLKAGADRELFARMLLEIYLLRRPDLTIVDGILAMEGDGPGSGDPRLCGLLLAGTNPVAVDVIAAEL
AGIPKKLLWVERAAEKLGIEGWDRATIDTRGVPVDEAKVSPFRLPHISDVQFGLPRFLKNRLRHYLTSRPCGNPEACTLC
GICRDACPPGAITIQDGKLAFDYHACIRCFCCRELCPEGALGVREGALLKMIKKCV
>Mature_376_residues
MRHAVAIERAGDYQSDVVRPALDRLLEPFGGMASFVKPGERVLLKPNMLAAKEPERAVTTHPELLRQVIGLVREAGGIPL
VGDSPGVGGIRRVAERSGILAVVEETGAEFIPFDETVTVRGKGLFREFQIARPYLEADRLINLPKLKTHEMMTMTCAVKN
LFGAVVGAAKAGWHLKAGADRELFARMLLEIYLLRRPDLTIVDGILAMEGDGPGSGDPRLCGLLLAGTNPVAVDVIAAEL
AGIPKKLLWVERAAEKLGIEGWDRATIDTRGVPVDEAKVSPFRLPHISDVQFGLPRFLKNRLRHYLTSRPCGNPEACTLC
GICRDACPPGAITIQDGKLAFDYHACIRCFCCRELCPEGALGVREGALLKMIKKCV

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: COG2006

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007160 [H]

Pfam domain/function: PF04015 DUF362 [H]

EC number: NA

Molecular weight: Translated: 40903; Mature: 40903

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00198 4FE4S_FERREDOXIN ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHAVAIERAGDYQSDVVRPALDRLLEPFGGMASFVKPGERVLLKPNMLAAKEPERAVTT
CCCCEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCCCCCCCHHHCC
HPELLRQVIGLVREAGGIPLVGDSPGVGGIRRVAERSGILAVVEETGAEFIPFDETVTVR
CHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEE
GKGLFREFQIARPYLEADRLINLPKLKTHEMMTMTCAVKNLFGAVVGAAKAGWHLKAGAD
CCCHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCC
RELFARMLLEIYLLRRPDLTIVDGILAMEGDGPGSGDPRLCGLLLAGTNPVAVDVIAAEL
HHHHHHHHHHHHHHCCCCCEEECCEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHH
AGIPKKLLWVERAAEKLGIEGWDRATIDTRGVPVDEAKVSPFRLPHISDVQFGLPRFLKN
HCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHCHHHHHHH
RLRHYLTSRPCGNPEACTLCGICRDACPPGAITIQDGKLAFDYHACIRCFCCRELCPEGA
HHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCEEEHHHHHHHHHHHHHHCCCCC
LGVREGALLKMIKKCV
CCCCCCHHHHHHHHHC
>Mature Secondary Structure
MRHAVAIERAGDYQSDVVRPALDRLLEPFGGMASFVKPGERVLLKPNMLAAKEPERAVTT
CCCCEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCCCCCCCHHHCC
HPELLRQVIGLVREAGGIPLVGDSPGVGGIRRVAERSGILAVVEETGAEFIPFDETVTVR
CHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEE
GKGLFREFQIARPYLEADRLINLPKLKTHEMMTMTCAVKNLFGAVVGAAKAGWHLKAGAD
CCCHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCC
RELFARMLLEIYLLRRPDLTIVDGILAMEGDGPGSGDPRLCGLLLAGTNPVAVDVIAAEL
HHHHHHHHHHHHHHCCCCCEEECCEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHH
AGIPKKLLWVERAAEKLGIEGWDRATIDTRGVPVDEAKVSPFRLPHISDVQFGLPRFLKN
HCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHCHHHHHHH
RLRHYLTSRPCGNPEACTLCGICRDACPPGAITIQDGKLAFDYHACIRCFCCRELCPEGA
HHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCEEEHHHHHHHHHHHHHHCCCCC
LGVREGALLKMIKKCV
CCCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]