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The map label for this gene is dsbA [H]

Identifier: 78222592

GI number: 78222592

Start: 1544746

End: 1545411

Strand: Direct

Name: dsbA [H]

Synonym: Gmet_1380

Alternate gene names: 78222592

Gene position: 1544746-1545411 (Clockwise)

Preceding gene: 78222585

Following gene: 78222593

Centisome position: 38.64

GC content: 56.61

Gene sequence:

>666_bases
ATGTCATTCAAATTTGTCAAAACAATCGCCAAAAGTCTCGCACTGGTAGCTCTGTTCGCCGGCAGCTGCTTCGCCTTCAC
CGAGGGGACCGATTACGTGAAGCTTGCGAAACCGATCCCGAACGCCCAGGGGACGCTGATCAAGGTGTTCAGCTACGACT
GCCCCTTCTGCTACAAGTACGACAAGAAGATCACGCCGAACCTGGTTCCCAAGCTGCCGAGCGATCTCAAGTTCCGCCCC
TTCCATCTGAAGACCAAGGGTAAATACGGCGTCCAGGGAAGCGAGCTCTTCGCCGTGCTGCTGCTCAAGGATCAGAAGGC
CGGCCTCTCCGACCGCGATCTGTATGGCCCCAAGTCACTCCTGAAGAAGGCCAAGATGGCCTACTACACCGCCTACCACG
ACAAGAAAGAGCGCTGGGATGCGGGCCCCGACGCCTACCTGAAGACCGGTCTCGACGCAGTCGGCATGAGCAAAGCAGAA
TTCGACAAGGCGAAGGCAGATCCCAAGGTCAAGGCGCTGCTGAAGGAGTGGGATGCCTCCTATGACGTGGCAAAGGTCCA
GGGCGTTCCCGGCTTCGTCGTCAACGGCAAGTACCTCATCATGACCAAGAGCATCACCTCCATAGACGGCATGCTGAAGC
TTATCAACGAGCTGAAGACCAAATAA

Upstream 100 bases:

>100_bases
CCAGGCCAGCAATCGGAGAAAGGAGGAGAACATCACACACCACACGTCATACGAGGCCAGGATTTGCAGAGCCAACTACA
CACTATCAAGGAGAAAGATC

Downstream 100 bases:

>100_bases
GGAGACGCGTCGTGAAACTATCAGAAATGTTCGGCAACTTCAGGTCGGACCCGGCAGGAACCATCAGCCAGTGGCAGGAC
AAGCGGTTTCTCTGGATCTT

Product: DSBA oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MSFKFVKTIAKSLALVALFAGSCFAFTEGTDYVKLAKPIPNAQGTLIKVFSYDCPFCYKYDKKITPNLVPKLPSDLKFRP
FHLKTKGKYGVQGSELFAVLLLKDQKAGLSDRDLYGPKSLLKKAKMAYYTAYHDKKERWDAGPDAYLKTGLDAVGMSKAE
FDKAKADPKVKALLKEWDASYDVAKVQGVPGFVVNGKYLIMTKSITSIDGMLKLINELKTK

Sequences:

>Translated_221_residues
MSFKFVKTIAKSLALVALFAGSCFAFTEGTDYVKLAKPIPNAQGTLIKVFSYDCPFCYKYDKKITPNLVPKLPSDLKFRP
FHLKTKGKYGVQGSELFAVLLLKDQKAGLSDRDLYGPKSLLKKAKMAYYTAYHDKKERWDAGPDAYLKTGLDAVGMSKAE
FDKAKADPKVKALLKEWDASYDVAKVQGVPGFVVNGKYLIMTKSITSIDGMLKLINELKTK
>Mature_220_residues
SFKFVKTIAKSLALVALFAGSCFAFTEGTDYVKLAKPIPNAQGTLIKVFSYDCPFCYKYDKKITPNLVPKLPSDLKFRPF
HLKTKGKYGVQGSELFAVLLLKDQKAGLSDRDLYGPKSLLKKAKMAYYTAYHDKKERWDAGPDAYLKTGLDAVGMSKAEF
DKAKADPKVKALLKEWDASYDVAKVQGVPGFVVNGKYLIMTKSITSIDGMLKLINELKTK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 24590; Mature: 24459

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFKFVKTIAKSLALVALFAGSCFAFTEGTDYVKLAKPIPNAQGTLIKVFSYDCPFCYKY
CCHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCCCCCEEEEEECCCCEEEEC
DKKITPNLVPKLPSDLKFRPFHLKTKGKYGVQGSELFAVLLLKDQKAGLSDRDLYGPKSL
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHEEEEEEEECCCCCCCCCCCCCHHHH
LKKAKMAYYTAYHDKKERWDAGPDAYLKTGLDAVGMSKAEFDKAKADPKVKALLKEWDAS
HHHHHHHHHHHHCCCHHHCCCCCCHHHHHCCCHHCCCHHHHHHHCCCHHHHHHHHHHCCC
YDVAKVQGVPGFVVNGKYLIMTKSITSIDGMLKLINELKTK
CCEEEECCCCCEEECCEEEEEECCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFKFVKTIAKSLALVALFAGSCFAFTEGTDYVKLAKPIPNAQGTLIKVFSYDCPFCYKY
CHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCCCCCEEEEEECCCCEEEEC
DKKITPNLVPKLPSDLKFRPFHLKTKGKYGVQGSELFAVLLLKDQKAGLSDRDLYGPKSL
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHEEEEEEEECCCCCCCCCCCCCHHHH
LKKAKMAYYTAYHDKKERWDAGPDAYLKTGLDAVGMSKAEFDKAKADPKVKALLKEWDAS
HHHHHHHHHHHHCCCHHHCCCCCCHHHHHCCCHHCCCHHHHHHHCCCHHHHHHHHHHCCC
YDVAKVQGVPGFVVNGKYLIMTKSITSIDGMLKLINELKTK
CCEEEECCCCCEEECCEEEEEECCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10600454 [H]